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The Effect of Turmeric Decoctum to the Angiogenic Molecules Expression on Chicken Embryo Zahariah, Sultanah; Winarsih, Sri; Baktiyani, Siti Candra Windu; Rahardjo, Bambang; Kalsum, Umi
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.10

Abstract

Turmeric (Curcuma longa) is widely used as herbal medicine, not an exception by pregnant women. Turmeric consumption by expectant mothers requires standard dose, because of its antiangiogenic effect could be harmful on placentation process and embryonic development. This experiment was undertaken to determine the effect of different concentrations of turmeric decoctum to the expression of Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) and Angiopoietin 1 (Ang-1) on the 48-hours-old chicken embryo. In this study, turmeric was extracted using decoction method to mimic the common method as adopted by people. The turmeric decoctum were freeze dried into a powder form and was used in preparing the stock solution for 200 ppm (P1), 300 ppm (P2), and 400 ppm (P3) as experimental treatments. The control group (P0) received 2% DMSO without turmeric decoctum. These were administered on the yolk sack of 16 hours incubation of fertile chicken egg by number of 200 µL. After 48 hours incubation, the expression of VEGFR-2 and Ang-1 on the chicken embryo were counted by ImageJ software. The results revealed that there is no significant effect of turmeric decoctum to the expression of VEGFR-2 and Ang-1. This suggested that turmeric decoctum was safe up to 400 ppm on chicken embryo.
Group A β-hemolytic Streptococcus Detection Using Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG) Alluza, Hamid Hunaif Dhofi; Mayasari, Ema Dianita; Prawiro, Sumarno Reto; Winarsih, Sri
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.02

Abstract

Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p < 0.05). The result showed that there was a difference in humoral immune response (IgG) between the groups albeit the difference was not significant (p > 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria. 
p53/Surviving Ratio as a Parameter for Chemotherapy Induction Response in Children with Acute Myeloid Leukemia Lenggana, Rinaldi; Nugroho, Susanto; Winarsih, Sri
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.02

Abstract

Acute myeloid leukemia (AML) is a malignancy that is often found in children. Many studies into the failure of apoptosis function, or programmed cell death, is one of the most important regulatory mechanisms of cellular hemostasis which is closely linked to the development of cancer, are important. Also, regulation of the apoptotic (p53) and anti-apoptotic (surviving) proteins influence treatment outcome. One role of p53 is to monitor cellular stress necessary to induce apoptosis. Surviving (BIRC5) is a group of proteins in the apoptosis inhibitor which works by inhibiting caspase-3. The role of surviving is considered very important in oncogenesis proliferation and cell growth regulation. Chemotherapy in childhood AML can inhibit cell growth and induce slowing as well as stopping the cell cycle. Thus, the aim of this study was to compare p53 and surviving before and after receiving induction chemotherapy in children with AML and also to determine the p53/surviving ratio. Peripheral blood mononuclear cells were collected from AML children before treatment and three months after starting their induction therapy. p53 and surviving were measured by flowcytometry using monoclonal antibodies. Data were analyzed by t-test for comparison between groups and Spearman’s test to find out the correlation between variables with a significant value of p < 0.05. A total of 8 children were evaluated. The intensity of p53 expression was not significantly increased after induction phase chemotherapy (p = 0.224), but surviving expression and the ratio of p53/surviving were significantly increased in the treatment group compared with the levels prior to chemotherapy (p = 0.002, p = 0.034), and there was a strong negative correlation between p53 and surviving after chemotherapy (r = −0.63, p = 0.049).
p53/Surviving Ratio as a Parameter for Chemotherapy Induction Response in Children with Acute Myeloid Leukemia Rinaldi Lenggana; Susanto Nugroho; Sri Winarsih
Journal of Tropical Life Science Vol. 6 No. 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.02

Abstract

Acute myeloid leukemia (AML) is a malignancy that is often found in children. Many studies into the failure of apoptosis function, or programmed cell death, is one of the most important regulatory mechanisms of cellular hemostasis which is closely linked to the development of cancer, are important. Also, regulation of the apoptotic (p53) and anti-apoptotic (surviving) proteins influence treatment outcome. One role of p53 is to monitor cellular stress necessary to induce apoptosis. Surviving (BIRC5) is a group of proteins in the apoptosis inhibitor which works by inhibiting caspase-3. The role of surviving is considered very important in oncogenesis proliferation and cell growth regulation. Chemotherapy in childhood AML can inhibit cell growth and induce slowing as well as stopping the cell cycle. Thus, the aim of this study was to compare p53 and surviving before and after receiving induction chemotherapy in children with AML and also to determine the p53/surviving ratio. Peripheral blood mononuclear cells were collected from AML children before treatment and three months after starting their induction therapy. p53 and surviving were measured by flowcytometry using monoclonal antibodies. Data were analyzed by t-test for comparison between groups and Spearman’s test to find out the correlation between variables with a significant value of p < 0.05. A total of 8 children were evaluated. The intensity of p53 expression was not significantly increased after induction phase chemotherapy (p = 0.224), but surviving expression and the ratio of p53/surviving were significantly increased in the treatment group compared with the levels prior to chemotherapy (p = 0.002, p = 0.034), and there was a strong negative correlation between p53 and surviving after chemotherapy (r = −0.63, p = 0.049).
Group A β-hemolytic Streptococcus Detection Using Anti-Outer Membrane Protein (OMP) Immunoglobulin G (IgG) Hamid Hunaif Dhofi Alluza; Ema Dianita Mayasari; Sumarno Reto Prawiro; Sri Winarsih
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.02

Abstract

Streptococcal pharyngitis sequel such as Rheumatic Fever (RF) or Rheumatic Heart Disease (RHD) is an autoimmune response mediated by T cells and IgG. Since it is an autoimmune process, the result of bacterial culture as the gold standard of diagnosis often shows negative results. IgG against the 33 kDa OMP is considered as an important mediator in the process of these autoimmune diseases, so its presence in blood serum can be used as a diagnostic tool. The purpose of this study is to prove that 33 kDa OMP is one of the immunogenic parts of the Streptococcus Group A β-hemolytic, so it is expected that IgG anti-33 kDa OMP can recognize and respond the bacteria and to support the probability of the Streptococcus Group A β-hemolytic infection. This study was a laboratory experimental study with a control group design. Animal used was RattusNovergicus immunized with whole cell bacteria or 33 kDa OMP mixed with Complete Freund’s Adjuvant or Incomplete Freund’s Adjuvant. Polyclonal IgG was obtained by drawing blood serum from the animals after immunization with Streptococcus Group A β-hemolytic for 4 weeks (A; n = 5) and 8 weeks (B; n = 5) or immunization with OMP 33 kDa for 4 weeks (C; n = 5) and 8 weeks (D; n = 5) and also negative control group (E; n = 5). Immunological tests were done using Dot Blot assay, ELISA, and immunocytochemical examination. The data obtained was then evaluated with statistical tests Kruskal-Wallis, Mann-Whitney and Repeated ANOVA (p < 0.05). The result showed that there was a difference in humoral immune response (IgG) between the groups albeit the difference was not significant (p > 0.05). Dot Blot and immunocytochemical tests indicated that IgG anti-33 kDa OMP were able to recognize and respond the Streptococcus Group A β-hemolytic antigen. This study concluded that 33 kDa OMP was the immunogenic part of the bacteria and that IgG anti-33 kDa OMP could recognize and respond the Streptococcus Group A β-hemolytic bacteria. 
The Effect of Turmeric Decoctum to the Angiogenic Molecules Expression on Chicken Embryo Sultanah Zahariah; Sri Winarsih; Siti Candra Windu Baktiyani; Bambang Rahardjo; Umi Kalsum
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.10

Abstract

Turmeric (Curcuma longa) is widely used as herbal medicine, not an exception by pregnant women. Turmeric consumption by expectant mothers requires standard dose, because of its antiangiogenic effect could be harmful on placentation process and embryonic development. This experiment was undertaken to determine the effect of different concentrations of turmeric decoctum to the expression of Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) and Angiopoietin 1 (Ang-1) on the 48-hours-old chicken embryo. In this study, turmeric was extracted using decoction method to mimic the common method as adopted by people. The turmeric decoctum were freeze dried into a powder form and was used in preparing the stock solution for 200 ppm (P1), 300 ppm (P2), and 400 ppm (P3) as experimental treatments. The control group (P0) received 2% DMSO without turmeric decoctum. These were administered on the yolk sack of 16 hours incubation of fertile chicken egg by number of 200 µL. After 48 hours incubation, the expression of VEGFR-2 and Ang-1 on the chicken embryo were counted by ImageJ software. The results revealed that there is no significant effect of turmeric decoctum to the expression of VEGFR-2 and Ang-1. This suggested that turmeric decoctum was safe up to 400 ppm on chicken embryo.
The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary Riny Natalina; Tatit Nurseta; Sri Winarsih
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.09

Abstract

Pellucide zone 3 (ZP3) involves in fertilization mechanism. Moreover, antibody of ZP3 can develop to inhibit egg and sperm interaction. This study aims to determine the effect of hZP3 (mab-hZP3) monoclonal antibody on the expression of Bcl-2 and Bax in follicle granulosa cells of the mice ovary. Female mice BALB/c were divided into 12 groups which consisted of control and experimental treatment group. Each group was added with 30% of total mice as error sample (1 mice). Each groups were treated differently: 50 µl adjuvant Al(OH)3 in 50 µl Tris HCl, 20 µg Mab-hZP3, 40 µg Mab- hZP3, and 60 µg Mab-hZP3. Each group was dissected at day 10, 15 and 20. Measurement of Bcl-2 and Bax was performed with immunohistochemistry. Data was analyzed by Two-Way ANOVA. There was no significant effect of Mab-hZP3 administration in various doses on Bcl-2 (p=0.0825), and Bax (p=0.836). There was no significant effect of administration of Mab-hZP3 in time (p=0.807), neither on Bcl-2 expression (p=0.088) and Bax (p=0.227). The lowest Bcl-2 level was found in dose of 60 µg in day 15. There was no significant effect of Mab-hZP3 in various doses and time (p=0.691), neither to Bcl-2 and Bax. Such results obtained due to the specificity of a monoclonal antibody that recognizes specific antigen. Mab-hZP3 is proposed as immunocontraception for women causing no disturbance of folliculogenesis.
Design, Construction and Expression of Spike Highly Conserved Region (HCR) SARS-CoV-2 and Cholera Toxin Subunit B Fusion Protein in Lactococcus lactis NZ3900: Construction of recombinant plasmid in Lactococcus lactis Kesuma, Suryanata; Adianingsih, Oktavia Rahayu; Winarsih, Sri; Widodo, Nashi; Yurina, Valentina
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/2xf1qp96

Abstract

Prevention of SARS-CoV-2 transmission has primarily been achieved through vaccination, which is generally administered via injection and may cause discomfort. No commercially available SARS-CoV-2 vaccines can be administered via the mucosal route. However, recent advancements have demonstrated that vaccination with Lactococcus lactis enables vaccine delivery through the mucosa. A promising target for SARS-CoV-2 vaccine materials is the highly conserved region (HCR) of the SARS-CoV-2 spike (SARS-CoV-2 HCR Spike). Vaccine efficacy is enhanced by adding Cholera Toxin Subunit B (CTB) as an adjuvant. HCR and CTB proteins were recombinantly fused using a synthetic gene with optimized codons. This study aimed to construct a fusion protein of the SARS-CoV-2 spike protein and CTB in L. lactis strain NZ3900. The construction and expression of fusion proteins were analyzed using sequencing and protein electrophoresis. Codon optimization resulted in a Codon Adaptation Index value of 0.93 and a GC content of 27.06%. The cloning results revealed the formation of L. lactis colonies expressing the Fusion protein of the SARS-CoV-2 HCR Spike and CTB, which formed yellow colonies on the selection Elicker medium. PCR and sequencing confirmed the presence of the hcr-ctb gene, with a length of 981 bp and 100% sequence similarity. The Fusion protein of the SARS-CoV-2 HCR Spike and CTB was successfully expressed with a molecular weight of >35 kDa. In conclusion, we successfully constructed a Fusion protein of the SARS-CoV-2 HCR Spike and CTB in L. lactis NZ3900 as a potential vaccine candidate for oral administration to prevent SARS-CoV-2 infection.
Co-Authors -, Kusdianti A, Aulanniam Abd. Haris Abd. Rasyid Syamsuri Ade Zakiya Tasman Munaf Adisty Dwi Treasa Ahmad Alim Bachri Ajeng Maharani Putri Akbariyanto, Fahrizal Akhid Yulianto Alfatah, Abu Hasan Alfian Wika Cahyono Alluza, Hamid Hunaif Dhofi Almira, Rehan Amelia, Putri Nurhidayati Amir, Nayla Nabilla Tahta Avwina Anastasia Shinta Wardhani Andy Soegianto Anggawirya, Arin Mantara Anggita Maharani ANISYAH ACHMAD Ariel, Dio Giovanni Arif Widagdo Arsy Arundina Aulanni'am, Aulanni'am Aulia, Aida Salsabila Ayangsari Cahyaningrum Ayu Nirmala Sari Ayuk Lawuningtyas Hariadini Azzahra, Ika Prima Bambang Rahardjo Burhan, M. Cahyaningtyas, Tita Dwi Cahyono, Alfian Wika Chatarina Umbul Wahyuni Daely, Brian Fo’era-era Danik Agustin Purwantiningrum, Danik Agustin Derisna, Anak Agung Dewi Indiastari DEWI SARTIKA Dhany Efita Sari Diningrat, Diky Setya Dio Giovanni Ariel Doris Noviani Dwi Yuni Nur Hidayati Elfi Anis Saati Ema Dianita Mayasari Ema P. Yunita Ema Pristi Yunita Ema Pristi Yunita, Ema Pristi Erwan, Nabila Erina Eviana Norahmawati Eviana Norahmawati Fachrul Islam, Ruckmana Febriani, Amelia Dwi Fitria Febriliani Putri Fitria Jannatul Laili Florensy Sauhenda, Angla Frenty Hadiningsih, Eka Frista, Ardaleni Hamid Hunaif Dhofi Alluza Hamida, Maretha Hanif Alamudin Manshur Hanur, Binti Su’aidah Harahap, Novita Sari Hariyani, Tintin Harjoni Harjoni Hasrun, Andi Hastuti, Nur Aini Retno Heidir, Hilwa Hidayah, Eva Nur Huwae, Thomas Erwin C.J I Nengah Kundera Iin Tri Marlinawati Ika Ratnaningrum Insania, Chika Janah, Uzlifatul Kalsumy, Umi Kana Mardhiyyah Karfin Kartika, Bintang Karyono Mintaroem Kesuma, Suryanata Khasanah, Putri Yatun Kinasih, Anggraini Kurotul Aeni Kuswanto Kuswanto Lanlan Muhria Lenggana, Rinaldi Lia Sawitri Loeki Enggar Fitri Louise Panggabean, Helena Maemunah Maemunah Majid, Muhammad Abdul Manuhutu, Natalia Mardhiyyah, Kana Marini . Marni Bawawa Marnina Marnina Maulota, Sally Inggrid Mayasari, Ema Dianita Melati Puspita Sari Muchammad Subali Noto Mukaromah, Lisa Aminatul Mukhamad Nooryanto Mukhlisah, Rafikah Munayarokh Munayarokh, Munayarokh Murdiono, Jatmiko Murtiningsih Murtiningsih Musa, Pabali Nabila Erina Erwan Nabilah, Aviana Zuhrotun Narahawarin, Margaretha F Narahawarin, Margaretha F. Nashi Widodo Nikmawati, Nuril Nisa R. Deasury Noorhamdani Novanda, Shabilla Caesar Nugraha, Rivo Yudhinata Brian Nur komalasari Nurhakim Nurhakim Nursodik, Tabah Nurul Istiqomah Oktavia Rahayu Adianingsih Palastri, Aisha Rifda Pande Made Dwijayasa Permana, David Galuh Prawiro, Sumarno Reto Prihannensia, Maydia Purwantiningrum, Danik Agustin Purwati, Ajeng Budi Putri Ardela, Mayasari Putri, Ajeng Maharani Putri, Fitria Febriliani Rahman, Chientya Annisa Rahmani, Nuraida Fara Ratnasari, Aprilya Seruni Retty Ratnawati Ribkha Itha Idhayanti Rinaldi Lenggana Riny Natalina Ririn Handayani Rista, Nadia Rivo Yudhinata Brian Nugraha Rosalia Sanarto Santoso Sani, Indra Santoso, Putu Adi Saputra, Hendriek Farhan Saputra, Ridwan Cahya Saputra, Wahyu Joko Sarnita Septiana, Celsy Citra Setyono Yudo Tyasmoro Shafira, Hanna Sijabat, Survey Siti Candra Windu Baktiyani Siti Candra Windu Baktiyani Siti Chunaeni Siti Nurhidayah Sri Andarini Sri Poeranto, Sri Sri Sukasih, Sri Sulistiyani Sulistiyani Sultanah Zahariah Sumarno Reto Prawiro SURYANTI Susanto Nugroho Susanto Nugroho Sutrisno Sutrisno Tamara Gusti Ebtavanny Tatit Nurseta Tatit Nurseta Tatit Nurseta Tatong Harijanto, Tatong Thomas Erwin Christian Junus Huwae Tita Hariyanti Tri Ardyati Tri Yudani Mardining Raras Triana, Salma Ayuning VALENTINA YURINA Wachid, Moch. Wahyuniar Wardhani, Anastasia Shinta Wibowo, Fiska Almayda Widayanti, Isyania Widya, Reta Anggraeni Wirayuda, Rangga Wiyasa, I Wayan Arsana Yogi Sugito Yohanes Kristantyo Yudhistira Ardana, Yudhistira Yudi Arimba Wani Yulistiani Yulistiani, Yulistiani Zahariah, Sultanah