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Journal : Bioscientist : Jurnal Ilmiah Biologi

Eksplorasi Bakteri-bakteri Pemfermentasi dalam Beberapa Produk Tempe di Kota Padang Nurmiati, Nurmiati; Periadnadi, Periadnadi; Jadespi, Sherly; Apriyelita, Annisa
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11094

Abstract

Tempeh is one of Indonesia's traditional foods produced through the microbial fermentation process of Rhizopus. It has become a favorite among the community, typically cooked before consumption. The fermentative bacteria in tempeh are suspected to act as probiotics, potentially making tempeh a functional food. Thus far, there have been no reports on the presence of fermentative bacteria in soybean tempeh in Padang City. Therefore, this study aimed to analyze the presence of fermentative bacteria and enzymatic activities in various tempeh products in Padang City. The research focused on identifying the presence and proportion of fermentative bacteria in soybean tempeh in Padang City. The study utilized a survey method, and the data obtained were analyzed descriptively. The results indicated the total presence of bacteria in soybean tempeh samples (11.2 - 15.3 x 10^6 CFU/g), fermentative bacteria (3.30 - 6.10 x 10^6 CFU/g), acetic acid bacteria (0.90 - 1.50 x 10^5 CFU/g), proteolytic bacteria (2.10 - 3.70 x 10^6 CFU/g), cellulolytic bacteria (34.60 - 6.50 x 10^6 CFU/g), and amylolytic bacteria (2.20 - 4.90 x 10^6 CFU/g).
Potensi Antimikroba dan Antioksidan Beberapa Ekstrak Daun Benalu (Scurrula ferruginea (Roxb. ex Jack) Danser dari Archidendron sp. terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans Apriyelita, Annisa; Periadnadi, Periadnadi; Nurmiati, Nurmiati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11108

Abstract

Scurrula ferruginea (Roxb. ex Jack) Danser is known for its role as an antimicrobial and antioxidant agent due to its content of phenolic compounds, alkaloids, and terpenoids. S. ferruginea has been widely used in traditional medicine for skin infections, diarrhea, hypertension, and digestive tract diseases. This study aims to determine the antimicrobial and antioxidant potential of various treatments of S. ferruginea mistletoe extracts. The experimental method used in this research is the nested pattern experiment. The extract treatments applied include fresh extract, boiled dry extract, boiled fresh extract, brewed dry extract, and brewed fresh extract. Antimicrobial potential was assessed using disc diffusion and dilution methods. Antioxidant activity (IC50) was determined using the DPPH (1,1-Diphenyl-2-Picryl-Hydrazine) method, and Total Phenolic Content (TPC) was also determined. The results indicate that the highest antimicrobial activity against Escherichia coli and Staphylococcus aureus was found in the strong category of fresh extract treatment, whereas there was no significant effect on Candida albicans growth. The fresh extract treatment exhibited the highest antimicrobial activity with a total phenolic content of 20.77321 mgGAE/mL. The Minimum Inhibitory Concentration (MIC) values for the fresh extract against S. aureus and E. coli were 6.25%, and the Minimum Bactericidal Concentration (MBC) values were 12.5% against both bacteria. The antioxidant value of the fresh extract was 101.26 μg/mL, categorized as moderate. Based on the conducted research, it can be concluded that S. ferruginea leaf extract can inhibit the activities of S. aureus and E. coli, while showing no effect on Candida albicans growth.
Eksplorasi Mikroflora Alami Produk Fermentasi Tradisional Cangkuak Berbasis Daging di Kecamatan Kuantan Mudik Kabupaten Kuantan Singingi Putri, Adela; Nurmiati, Nurmiati; Periadnadi, Periadnadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11216

Abstract

Cangkuak is a fermented food made from meat with betung bamboo shoots or kluwak, salt and rice. The fermentation process is carried out without adding microbial cultures (spontaneous) for one to three weeks in a tightly closed container. cangkuak's manufacturing technique, ingredient formulation and constituent ingredients are unique, resulting in a final product with different characteristics. Research on the presence of microbes in fermented food products (fish, milk, vegetables) has been widely conducted, but information on exploring the presence of natural microflora and the in vitro potential of fermenting bacteria in cangkuak has never been reported. This research was conducted using a survey method and the resulting data were presented descriptively. It was found that the natural microflora found in the three samples of cangkuak in Kuantan Mudik was dominated by bacteria, with the highest proportion of bacterial groups in the three cangkuak samples being fermentative bacteria (2.0 - 6.30x105 cfu/g), cellulolytic bacteria (3.30 - 5.90x105 cfu/g), amylolytic bacteria (1.70 - 4.40x105 cfu/g), proteolytic bacteria (0.12 - 7.90x105 cfu/g), lipolytic bacteria (0.08 - 0.12x105 cfu/g). Six potential fermentative isolates were obtained as evidenced by the calculation of index values, namely DK-I1 (1.59), DR-I1 (1.36), DR-I2 (2.49), DR-I3 (1.19), DKR-I1 (2.41), and DKR-I2 (2.96).
Eksplorasi Mikroflora Alami Cairan Nepenthes mirabilis (Lour) Druce di Kawasan Hutan Penelitian dan Pendidikan Biologi Universitas Andalas Titami, Hania; Periadnadi, Periadnadi; Nurmiati, Nurmiati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11277

Abstract

Indonesia is a country that is rich in flora and fauna diversity, one of the unique flora in Indonesia is Nepenthes. This bag serves to trap insects or other small animals, the Biology Education and Research Forest (HPPB) is located in the Andalas Limau Manis University campus area which is classified as lowland tropical rain forest which is the habitat of Nepenthes, in the Biology Education and Research Forest (HPPB) this type of pitcher plant, namely Nepenthes mirabilis. Nepenthes mirabilis is a plant that is able to digest insects trapped in pockets at the ends of its leaf tendrils, so it is classified as a carnivorous plant. Microorganisms play an important role in breaking down organic materials into inorganic ones available to plants. Types of bacteria and bacterial populations greatly determine the decomposition process of animals that have been trapped in the bag as a source of nutrients for Nepenthes plants. This study aims to explore the natural microflora of Nepenthes mirabilis fluids, determine the proportional presence of potential bacteria, and determine the in vitro potential of chitinolytic bacteria from Nepenthes mirabilis fluids. This study used a survey method which was analyzed descriptively. The results showed that Nepenthes mirabilis fluid contained the presence of Fermentative, Amylolytic, Proteolytic, Chitinolytic and Lipolitical bacteria. Nepenthes mirabilis liquid in open bags contained chitinolytic bacteria, the highest presence of chitinolytic bacteria was found in MBB samples (20,104 cfu/ml) and MBA samples (18,104 cfu/ml). .28), fermentative (2.33 and 2.00), amylolytic (1.42 and 1.28), proteolytic (2.00 and 2.00), lipolytic (1.75 and 1.28).
Co-Authors Abd. Kadir Abdul Hamid Abubakar, Achmad Adiaksa, Baso Witman Agus Arman, Agus Ahmad Afiif, Ahmad Ahmad Fadli AHMAD JUNAEDI Akbar Akbar Ali, St. Aminah Aminuddin Prahatama Putra Amir Amir Andriani, Reni Anik Aspirin Anissa, Fausiyah Antasionasti, Irma Anthoni Agustien Aprianti, Ulfa Apriyelita, Annisa Arie Anggraini Arnianti Arnianti Asikin, Siti Badria` Asmayani, Asmayani Asmayani, Kaswari Asmin, Risna Yunita Asriana Asriana, Asriana Astin Nur Hanifah Audina, Zikkra Awaliah, Ainun ayu Kalista Bachtiar, Irmah Halimah Baharuddin Baharuddin Bambang Kusbandrijo Barung, Rachmat Cayati, Cayati Chandra Pribadi Dahelmi Dahelmi Deswati Deswati Devin, Devin Didi Susanto, Didi Dila Fitria Dirham, Izza Fadhlinah Djunaedi Djunaedi Djurumudi J, Khairunnisa Noervaqriani Dwiani, Afe Dwisa Putra, Wahyu Efrizal Efrizal eka rahmawati Ekaningtiyas, Maya Erliza Hambali Ernawati Ernawati Faig Aizy, Fayadh Farahdiba, Syarifah Farild, Miftha Fariz Pari, Rizfi Fauzia Bakhtiar Fauziah, Ayu Bella Febrianti, Magfirah Febrianti, Mifta Fery, Fery Fettriwanti, Fettriwanti Fina Diana Firman Firman Firmansyah Firmansyah FITRAH AMALIA Fitri Deswita Fitri, Nurul G, Hasriani Gafur, Gafur Gayatri, Wulan Gazali, Zulkarnain Gustina Indriati Hadijah Hadijah Hakim, Akhmad Nur Halizah Halizah Hamansah Hamansah, Hamansah Handayani, Eka Sri Handayani, Rani Harfa, Nuraliyah Azizah Harianto, Samiin Hadi Hartati, Winda Hasan, Phika Ainnadya Hayati, Sri Ayatina Hendra, Gabriel Septian Hendrianto Hendrianto, Hendrianto Hente, Asri Hery Fajeriadi Hojang, Basri M Husnul Madihah, Husnul Hutajulu, Petrus Oktavianus Ibrahim, Muhammad Akmal Ika Aryani Ilham Ilham Intan Pramita, Intan IRA NURIYA SANTI Irawati Irawati Irma Yanti Irmawati Irmawati Irmayani Irmayani Irwan Rahman, Irwan IRWANDA, IRWANDA Isdaryanti, Isdaryanti Ismail Ismail Ismail, Wahyuni Iwan Setiawan Jadespi, Sherly Jafar, Akhmad Jannah, Siti Wardatul Jeranah, Jeranah Jirana, Jirana Jufri, Fitriana Julianri Sari Lebang, Julianri Sari Jumriani, Jumriani Jusniaty, Jusniaty Kamaruddin, Muh. Ihsan Kamran, Muhammad Karim, Mutemainna Khairunnisa Khairunnisa Kunaifi Kunaifi Kuswara, R. Didi L, IDRUS Leli, Leli Lia Masefa, Lia Listari, Nening Lubis, Bastian Lume, Lume M Said Abdullah Mades Fifendy Mahmudah, Yulia Nasibatul Marhaeni, Marhaeni Marwah, Syarrafah Masrah Masrah Maya Ekaningtias Mayasari, Rini MENAUNG, FANDY Mesra Damayanti Metatia Monica Mildaratu, Mildaratu Mildawati Mildawati Mira Nila Kusuma Dewi Mudinillah, Adam Muh. Alfi Syahrin Muhammad Aqil Muhammad Arsyad Muhammad Danial Muhammad Rizaldi Trias Jaya Putra Nurdin Muhammad Sofyan Muhammad Zaini Munir, Abd. Murbayani Murbayani, Murbayani Murniati . Nadiya, Zulfa Nasir, Yusrianto Nelson Nelson Nenny Indrawati, Nenny Nilah Ratnasari, Nilah Nirwana, Nirwana Nitasyawal, Mulyani Novieta, I. D. Nur Asiah Siregar Nur Faida, Nur Nur Wahyunianti Dahri Nurhidayah Nurhidayah Nurmina Nurmina, Nurmina Nursaeni Nursaeni, Nursaeni Nurwahyuni Parhani, Aan Pariyati, Pariyati Patimang, Andi Periadnadi Periadnadi Petir Papilo Pitriyani Pitriyani Pratiwi, Aliah Puspitaloka Mahadewi, Erlina Putra, Wahyu Dwisa Putri, Adela Putri, Andi Mega Januarti Qudratullah, Fyzria Rahmaddiansyah, Refa Rahmah, Andi Rahman, Sari Rahayu Rahman, Suburi Rahman, Sufirman Rahmawati, Agustini Liviana Dwi Rasbawati, Rasbawati raudatul jannah Rico, Rico Ridwan, Ridwan Rijal, Abdul Rahman Khaerul Riswanti, Riswanti Risya Saputri, Risya Rita Eka Izzaty, Rita Eka Rosdeviana, Rosdeviana Rostini Rusnam Rusnam Rusti, Sukarsi Rusydi, Bahrul Ulum Sabriana, Riska Sahli, Muh Sainah, Sainah Iin Samudin, Mohammad Tofan Sanjaya sanjaya Sari, Diah Kharisma Sari, Erma Puspita Sari, Wulan Komala Sembiring, Rinawati Septiani, Indri Sholehah, Dina Via Siampa, Jainer Pasca Siti Mariam Sjahruddin, Amran Solfiyeni Solfiyeni Srifitayani, Nur Rahma Suhartini, Eka Suhendra Suhendra Sulaeman Miru Sulistiani Sulistiani, Sulistiani Suloi, Andi Fitra Sumaryanti Sumaryanti Suparianto, Rishi Supiati Supiati Surtikanti Surtikanti Susanti Susanti Suwirmen, Suwirmen Syafrina, Vellin Putri Syamsiara Nur Syamsuddin, Naidin Titami, Hania Tohari, Sabit TONDATUAN, ALYCIA GRACE Treasa, Adisty Dwi tri wahyuni Tri Yulianti, Tri Ula, Zumrotul Ulfiani Rahman Waddi Fatimah Wahyudi Wahyudi Wahyuningsih Wahyuningsih Wayan Kantun Widya Anggrayesti Wijayanti, Lumastari Ajeng Wulandari, Andi Sri Rezky Yana Ameliana Yaqutunnafis, Lale Yaumi, Ema Nuzulul Yuliati, Ni Made Yunita Kristina Yunus, Ahyuni Zakaria, Indra Junadi Zelvia Misdar Zuhri Syam Zulkarnain, A.