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All Journal International Journal of Evaluation and Research in Education (IJERE) HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Media Penelitian dan Pengembangan Kesehatan Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) KEUNIS Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Pendidikan : Riset dan Konseptual ELSE (Elementary School Education Journal) : Jurnal Pendidikan dan Pembelajaran Sekolah Dasar Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Dinasti International Journal of Education Management and Social Science Jurnal Prakarsa Paedagogia Indonesian Journal of Tropical Aquatic JURNAL GEOGRAFI Geografi dan Pengajarannya ASANKA: Journal of Social Science And Education International Journal of Social Learning (IJSL) MAHESA : Malahayati Health Student Journal Abdi Masya Dharmas Education Journal (DE_Journal) Mejuajua El-Jughrafiyah : Jurnal Geografi dan Terapannya Golden Ratio of Mapping Idea and Literature Format Batara Wisnu : Indonesian Journal of Community Services Makara Journal of Science JURNAL PENGABDIAN PENDIDIKAN MASYARAKAT (JPPM) Medani : Jurnal Pengabdian Masyarakat Innovative: Journal Of Social Science Research Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Mutiara : Jurnal Ilmiah Multidisiplin Indonesia Jurnal Akuntansi, Keuangan, Perpajakan dan Tata Kelola Perusahaan International Journal of Geography, Social, and Multicultural Education (IJGSME) Jurnal Agripet Jurnal Riset Keperawatan dan Kesehatan Proceeding of the International Conference on Global Education and Learning
sri murtini .
Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Bogor, Jawa Barat, Indonesia 16680
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Journal : Makara Journal of Science

 1 
Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri
Makara Journal of Science Vol. 17, No. 1
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The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mxgene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.
SEROSURVEILLANCE OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 WITH HAEMAGGLUTINATION-INHIBITION ON WILD AQUATIC BIRDS IN PULAU DUA SERANG NATURAL RESERVES, BANTEN PROVINCE Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri; Noor, Yus Rusila
Makara Journal of Science Vol. 15, No. 2
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Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.
Chicken Immune Responses to Vaccination by the Avian Influenza Subtype H5N1 against Avian Influenza Ramlah, Ramlah; Murtini, Sri; Soejoedono, Retno Damayanti; Natih, Ketut Karuni Nyanakumari; Ningrum, Siti Gusti
Makara Journal of Science Vol. 27, No. 3
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Abstract

Avian Influenza (AI) is one of the strategic animal diseases still considered a priority for control by the Indonesian government. This study aimed to examine the immune response of chickens to various types of H5N1 subtype AI vaccines in Indonesia and to determine the correlation of factors influencing the post-vaccination AI antibody response. Serum samples were tested using the hemagglutination inhibition (HI) test with the standard AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) and strain A/Chicken/Semarang/04141225-07/2014 (Clade 2.3.2). Antibody titer was calculated using the Geometric Mean Titer (GMT). The correlation analyses were performed to assess the correlation of antibody titer against each of the following factors: age of chicken at the time of sampling, the interval between sampling time and the previous vaccination, and the number of vaccinations performed. The results showed that the average antibody titer value against the AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) was highest in samples from the South Sumatra Province, namely, Palembang City, which was 26.42 HIU. The results showed a significant correlation (p-value <0,05) between antibody titer and the interval between sampling time and the previous vaccination, the number of vaccination performed and age of chicken at the time of sampling. Therefore, results showed that the immunity developed from vaccination using the AI vaccine seed subtype H5N1 could induce immunity with a protective value of ≥16.
Co-Authors -, Mujiatun Abas, Farhat Abdul Kohar Abdul Zahid Adi Supryatno Adiningsih, Melani Wahyu Aep Saefullah Afiff , Usamah Agik Suprayogi Agung, Rizki Nugraha Agus Sutedjo Agus Sutejo Agus Suwondo Agus Wijaya Agustin, Riya Ahmad Biharidin Ahmad Furqon Ahmad Yusuf Aida Kurniawati Aisy, Izdihar Alen Ardiansyah Ali Ridho Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Almegi, Almegi Alzab, Amrie Muhammad Amrie Muhammad Andrijanto Hauferson Angi Angelina N. Tethool Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Arif Purwo Mihardi Asah Hilaliah Asep Surya Lesmana Ayi Santika Ayu Nurhania Ayundyayasti, Prima Bambang Hariyanto Bambang Sigit Widodo BAMBANG SIGIT WIDODO Bayu Febram Budi Prajogo Budiono, Novericko Ginger Cece Sumantri Christina, Budhi Adhiani Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dewi, Arum Kusnila Dock, Chanthoeurn Dwi Hany Yanti Dwi Retno Wulandari Edi Sukmawinata Edi Sukmawinata Edward, Syafri Eka Murtiasri Eko Budiyanto EKO BUDIYANTO Eko Sugeng Pribadi Ekowati Handharyani Elok Budi Retnani Etih Sudarnika Fadirubun, Fahmi Fahrudin Fadjar Satrija Fadjar Satrija Fahmi Fahrudi Fadirubun Fahrudin Fadirubun, Fahmi Fathin, Khusnun Fitria Susanti Fitria Susanti Fitriani, Hervina Gayatrie, Christina Retno Hasanudin, Mohammad Heirina, Anna HELMI -, HELMI Hendra Candra Hendriana, Okta Hilmy, Anggit Athoillah I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Ikhlasul, Galih Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Izdihar Aisy Jihan Rahmawati Joko Yunianto Prihatin Joned C. Saksana Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Ketut Prasetyo koekoeh santoso Kusaeri Kusaeri Latifa, Rima Leli Anggreini Leni Maylina Lesmana, Asep Surya Lestari Sitohang, Lidya Lestari, Alif Putra Lidya Lestari Sitohang Lismawati, Rina Listari, Tria Luci Cyrilla M.B.M Malole Made Agus Nurjana Makhmudiyah, Nurul Malonda Maksud Mansur, Ahmad Wafa Mardinawati, Mardinawati Marlefzena Marlefzena Marliyati Marliyati, Marliyati Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Asrori Muhammad Fikri Al Habib Muhammad Noor Ardiansah Muharifah, Isnaini Mujiatun, Mujiatun Murtono Murtono Nanis Nurhidayah Natih, Ketut Karuni Nyanakumari Neksidin Neksidin, Neksidin Nensy Tri Putri Niken Ulupi Norma, Getrina Putri Noviriliensi Hartika Novita, Yeni Noviyanti Noviyanti Nugraha, Arifin Budiman Nugraha, Kenda Adhitya Nugrahaeni, Mutiara Nugraheni, Dyah Hapsari Eka Nugroho Hari Purnomo Nurhayati Nurhayati Nurly Faridah Oktandini Sugesti Putri Okti Nadia Poetri Opyn Mananta Pambudi, Slamet Pamungkas, Joko Perdan Prasetya, Sukma Permana, Rangga Pradika, Jaka Prastiyono, Hendri Prima Ayundyayasti Purwaningsih Purwaningsih Putera, Alifi Restu Putra, Idrisi Raliya Putra, Raditya Pradana R Murwarni R. Susanti Raditya, Mochammad Nova Rafiq, Faizal Raharjani, Rani Rahmah, Ririn Ra'ifatur Ramadani Pardian Ramlah Ramlah Rani Raharjanti Ratnawati, Diana Ravinesh Rohit Prasad Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Setyaningsih Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Riki Siswandi Rindawati, Rindawati Risqika Akla Velayati Rita Mutia Ronald Tarigan Rosyadi, Humam Rotinsulu, Dordia Anindita Rudi Afnan Rudiansyah Rudiansyah, Rudiansyah RULLY ARDIANSYAH Ruvi, Muhammad Sabitah, Sabrina Azharia Sadida, Afiat Safitria Wulandari Salima, Rianti Samarang Samarang Sarana Sarana Satrija, Esdinawan Carakantara Saukhan, Ghiandra Naufal Syazily Sekar Ayu Chairunnisa Setiawan, Noor Rohman Silistyorini, Cahyo Ismawati Siregar, Fuad Siskamto Siti Gusti Ningrum, Siti Gusti Sitti Chadijah Sofiani, Yani Sri Darwati Sri Estuningsih Sri Nuryati Sri Utaminingsih Sriviyantiningrum, Nurul Sukma Perdana Prasetya Sulistinah Surachmi Setiyaningsih Suriadi Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Indri Nur Krisna TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tohiroh, Tohiroh Tria Lestari Triyono Triyono Ulfah Hidayati Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Vianisa, Putri Virgiawan, Dhimas Bagus Wahono Esthi Prasetyaningtyas Wirabumi, Putu Wiwien Mukti Andriyani Wiwien Mukti Andriyani Wiwik Sri Utami WIWIK SRI UTAMI YANUAR, ADY FITRA Yudhaningsih, Resi Yunitri, Ninik Yus Rusila Noor Yus Rusila Noor Yusuf Ridwan Zubair Arza