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Journal : Jurnal Veteriner

Profil Elektrolit Serum Pedet Sapi yang Diinfeksi Escherichia coli K-99 dan Diberi Mikrokapsul Imunoglobulin-G Anti E. coli Arief Purwo Mihardi; Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.791 KB) | DOI: 10.19087/jveteriner.2019.20.2.158

Abstract

Escherichia coli (E. coli) K-99 infection cause acute diarrhea in calves which may reduce electrolyte, such as sodium and potassium. The experiment was conducted to study the sodium and potassium profiles in calves infected by E. coli and received immunoglobulin G anti E. coli K99 microcapsules. Sixteen calves, 4 – 6 days years old, were grouped into four groups, consists of four calves, i.e. negative control/NC group (the calves were not infected by E. coli K-99 and no treatments); positive control/PC group (the calves were infected by E. coli K-99 and no treatments); treatment 1/P1 group (the calves were infected by E. coli K-99 and received colostrum suspension of IgG anti E. coli K-99); and treatment 2/P2 group (the calves were infected by E. coli K-99 and received microcapsules of IgG anti E. coli K-99). Escherichia coli K-99 were infected to all calves except the NC group orally with the dosage of 5 × 1010 cfu/ml. Suspension and microcapsules of IgG anti E. coli K-99 for each calf were given orally with dosage of 1.76 gram IgG anti E. coli K-99 per day. Serum were collected from jugular vein at 0, 24, 48, 72, and 168 hours after E. coli K99 infection. The sodium and potassium concentrastion were analyzed using a spectrophotometer. Results of the experiment indicated that the concentration of sodium and potassium were not significantly different among groups. Sodium concentration of P2 group was relatively higher than PC or P1 and showed increased concentration 168 hours after infection. Potassium concentration of P2 were higher than the PC group, but lower than NC and P1 group. In conclusion, the administration of IgG anti E. coli K-99 microcapsules was not significantly change the sodium and potassium concentration, but may maintain the stability of sodium level in calves infected by E. coli K-99.
Produksi IgY Antivirus Avian Influenza H5N1 dan Prospek Pemanfaatannya dalam Pengebalan Pasif I Wayan Teguh Wibawan; Sri Murtini; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.262 KB)

Abstract

Immunoglobulin Y (IgY) in yolk has been shown in several studies to prevent both bacterial and viralinfections. This research was conducted to find evidence that IgY specific against avian influenza virus(AIV) of H5N1 subtype can be produced in a large quantity in egg yolk. Laying hens were vaccinated withAI killed-vaccine (IPB-Shigeta). The IgY was purified using affinity chromatograpy technique, and anti-H5activity was measured using a standard haemaglutination inhibition (HI) and agar gel immunodifusion.The concentration of IgY was calculated, and the protein pattern was detected using polyacrilamid gel(AGID) electrophoresis (PAGE). Anti H5 antibody as high as 27 – 29 HI units was detected and produce aspecific line of precipitation in AGID. The concentration of IgY was 7.89 mg/ml. Purified specific IgY consistof 6 main protein bands with molecular weights ranging from 35 to 225 kD. These proteins were sensitiveto heat treatment (75oC for 30 minutes), to acid condition (pH2) as well as the pepsin and trypsin. Theseresults indicated the possibility of using specific IgY for passive immunisation to prevent AIV infection oras immunotherapeutic applications for AI treatment in humans.
Prevalensi dan Faktor Risiko Infeksi Parasit Saluran Pencernaan pada Kerbau Lumpur di Kabupaten Brebes, Jawa Tengah Nanis Nurhidayah; Fadjar Satrija; Elok Budi Retnani; Dewi Apri Astuti; Sri Murtini
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.598 KB) | DOI: 10.19087/jveteriner.2019.20.4.572

Abstract

Kerbau lumpur merupakan ternak multiguna yang memiliki kedekatan dengan kehidupan masyarakat di kawasan lumbung ternak khususnya di Kabupaten Brebes, Jawa Tengah. Infeksi parasit saluran pencernaan pada kerbau lumpur bedampak negatif terhadap kesehatan ternak sekaligus menyebabkan kerugian ekonomi. Data epidemiologi infeksi saluran pencernaan pada kerbau lumpur di Kabupaten Brebes belum tersedia. Penelitian ini bertujuan untuk mengukur prevalensi, derajat infeksi serta analisis faktor risiko infeksi parasit saluran pencernaan pada kerbau lumpur di Kabupaten Brebes. Sebanyak 240 sampel tinja diperoleh dari kerbau di empat kecamatan, yaitu; Salem, Bantarkawung, Tonjong dan Brebes. Hasil pemeriksaan mikroskopik dengan teknik Mc Master menunjukkan bahwa 27.5% kerbau terinfeksi oleh parasit saluran pencernaan. Infeksi disebabkan oleh Toxocara vitulorum (1.67%; telur tiap gram tinja [TTGT] 174.4), cacing Strongyle (Cooperia, Nematodirrus, Trichostrongylus) (4.17%; TTGT 140.18), Trichuris spp. (3.33%; TTGT 64.84), Moniezia expansa (9.17%; TTGT 122.47) dan Eimeria spp (21.25%; TTGT 162.37). Infeksi lebih tinggi tercatat pada kelompok jantan, umur muda (>12–30 bulan), kerbau di Kecamatan Salem dan yang dipelihara pada daerah bertipe iklim D4. Variabel umur merupakan satu-satunya faktor risiko infeksi yang ditunjukkan dengan ­P-value 0.03. Nilai odd ratio kelompok muda dan pedet adalah 3.424 dan 2.529, artinya, kelompok muda dan pedet memiliki risiko 3.424 dan 2.529 lebih tinggi dibandingkan kerbau dewasa (referensi). Dari penelitian ini dapat disimpulkan, baik prevalensi maupun derajat infeksi parasit saluran pencernaan di Kabupaten Brebes terjadi dalam taraf yang rendah dan dipengaruhi oleh faktor umur ternak.
Dinamika Total Protein Serum Tikus Putih (Rattus novergicus) yang Diberi Mikrokapsul Imunoglobulin-G Anti-H5N1 (DYNAMICS OF TOTAL PROTEIN SERUM OF RATTUS NOVERGICUS GIVEN MICROCAPSULES OF IMMUNOGLOBULIN G ANTI H5N1) Kathirina Beatrik Riwu Wolo; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.729 KB) | DOI: 10.19087/jveteriner.2019.20.4.504

Abstract

Immunoglobulin G (IgG) from colostrum of hyperimmune colostrum obtained from vaccinated cattle with H5N1 vaccine is potential to be developed into passive immunotherapy product for “bird flu” infection. The administration of passive therapy can be done orally, but the environmental conditions of the digestive tract can cause IgG damage, so the microencapsulation technique was used in this study to protect IgG. This study aimed to evaluate the absobrption of H5N1 IgG anti-Avian Influenza which was microencapsulated using rats (Rattus novergicus) as animals model. This study was conducted using 108 adult male white rats (Rattus norvegicus), Wistar strain, approx. 250 grams of body weight. Rats were divided into three treatment groups (N = 36), i.e. control group (K) were given aqua distilled water, suspension group (S) were given anti-H5N1 IgG suspension, and microcapsules group (M) were given microcapsules equivalent to suspension with the titer of 25. Necropsy was carried out at four observation points (2, 4, 6 and 8 hours) to collected blood through the heart and collected in vacutainer without anticoagulants to obtain serum. The blood serum then analyzed for total protein and globulin and albumin/globulin ratio (A/ G). The results showed that the level of total protein and globulin group M was higher and significantly different (P> 0.05) compared to K and S at the end point of observation. The A/G ratio in group K showed a significant increase (P> 0.05) at the end point of observation while in group M there was a marked decrease (P> 0.05) at the time point of 2 hours to 4 hours and then constant until the end of observation. These results showed that administration of anti-H5N1 IgG microcapsules can increase total levels of protein and globulin in the blood.
Respons Kekebalan Ayam IPB D1 yang Memiliki Gen TLR4 terhadap Infeksi Bakteri Salmonella enteritidis (IMMUNE RESPONSE OF IPB D1 CHICKENS WITH TLR4 GENES AGAINST SALMONELLA ENTERITIDIS BACTERIAL INFECTION) Fitria Susanti; Sri Murtini; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Faculty of Animal Science, IPB University has developed new trait of chicken named IPB D1 traits. The chicken is a crossbreed chicken between male F1 PS (Pelung x Sentul) with F1 female (Kampung x parent stock Cobb). The IPB D1 chickens aims to produce the local commercial meat type, which adapted to traditional or semi intensive management (back yard). Salmonellosis is one of the bacterial disease that commonly infected poultry, therefore a study was conducted to determine the immune response of IPB D1 chickens to these disease using genetic markers, namely TLR4 gene (Toll-Like Receptor 4). TLR4 gene is one of the genes that control chicken resistance to Gram negative bacteria infection, through non-specific immune responses, one of Gram negative bacteria is Salmonella enteritidis. The aim of this study was to determine of TLR4 gene and their role in immunity of IPB D1 chickens against S. enteritidis infection. As much as 11 chickens were use in this study. This research was carried out through several stages of i.e determination of the TLR4 gene by PCR and sequencing and observing its immune response through the number of leukocyte, leukocyte differentiation and other hematology profile. Humoral immunity response against S. enteritidis were observe by clearance test before and after challenged with S. enteritidis. The results of this study indicate that IPB D1 chickens which have TLR4 genes (genotype GG and AG) were resistance against S. enteritidis based on the leukocyte, hematology profile and humoral immunity.
Perbandingan Deteksi Titer Antibodi Pascavaksinasi Rabies Berbasis Kolorimetri Menggunakan ELISA Reader dan Kamera Telepon Genggam Koekoeh Santoso; Ulfatin Khoiriyah Herowati; Dordia Anindita Rotinsulu; Sri Murtini; Muhammad Yusuf Ridwan; Denny Widya Hikman; Abdul Zahid; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Agus Wijaya; Ridi Arif; Ronald Tarigan; Edi Sukmawinata
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.315 KB) | DOI: 10.19087/jveteriner.2021.22.1.79

Abstract

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.
Respons Kekebalan Tubuh Ayam IPB D1 terhadap Infeksi Virus Penyakit Tetelo (Newcastle Disease) (IMMUNE RESPONSE OF IPB D1 CHICKEN AGAINST NEWCASTLE DISEASE VIRUS ) Retno Setyaningsih; Sri Murtini; Okti Nadia Poetri; Cece Sumantri
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.58 KB)

Abstract

Newcastle disease (ND) or Tetelo is an endemic poultry disease in Indonesia, and caused significant economic losses. Instead of diseases control programme has been carried out, ND outbreaks still occurs among poultry flock. IPB D1 chicken is a type of cross-breeding chickens between pelung-sentul crossbred chicken male and kampung-broiler crossbred chicken female, this chicken is genetically selected based on on body resistance to disease. The aim of our research is to study immune response of IPB D1 against ND infection. Fourty IPB D1 chickens were used in this study, and divided into three groups : vaccinated group, unvaccinated group, control group. Vaccinated group received two time ND vaccination at age 10 weeks and booster at age 12 weeks. Unvaccinated and control group were remain not vaccinated. At age 13 weeks, vaccinated and unvaccinated group were challenge with ND virus (107 EID50/ml). Immune response were determined based on antibody titer which is measured by haemagglutination inhibition (HI) test and the profile of white blood cell of the chicken. Our result showed that both vaccinated and unvaccinated group has ND antibody titer e” log 24 at challenge time, and survived after challenge. White blood cell profile among all groups showed that lymphocites has the higher number than other leukocyte type. It can be concluded that IPB D1 chickens showed a good response against ND virus.
LYMNAEA SPP. SNAIL AS INTERMEDIATE HOST OF TREMATODE IN PRAFI DISTRICT, MANOKWARI, PAPUA BARAT Noviyanti Noviyanti; Angelina Novita Tethool; Purwaningsih Purwaningsih; Fadjar Satrija; Sri Murtini
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.555 KB)

Abstract

Paramphistomum spp. and Fasciola spp. are the most common trematodes that infect cattle throughout the world including in Manokwari, West Papua. During their life cycle, freshwater snails act as the intermediate host for the larval. This study aims to provide information about the presence of cercariae –the larval form of trematode- within the body of Lymnaea spp. snail. The study was conducted in August-September 2019 in Prafi District, Manokwari, West Papua. Lymnaea spp. were collected from 15 permanent and seasonal drainage around a water dam, rice field irrigation, and palm oil plantations. Snails are collected by hand using a quadrant or using the "man per minute" method. Cercaria within the snail's body is examined using the "crushing method" under a light microscope. Snails that have cercaria trematodes are categorized as infected snails. The percentage of snails infected with trematodes in August and September were 15.92% (53 out of 333 snails) and 38.05% (78 out of 205 snails), respectively. Cercariae that were identified were longifurcate-pharyngeate cercariae (Strigea cercaria), Cercariaeum cercaria, and Virgulate cercaria. The adult phase of these cercaria are generally exist in the intestine of mammals, amphibians, birds and fish. Further research is needed to provide more accurate information about the epidemiology and morphology of trematodes in freshwater snails.
Keberhasilan Hiperimunisasi Pada Sapi Bunting: Kajian Terhadap Ketepatan Waktu Vaksinasi Safitria Wulandari; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.565 KB) | DOI: 10.19087/jveteriner.2022.23.1.88

Abstract

Penelitian ini bertujuan untuk melihat pengaruh umur kebuntingan induk sapi saat vaksin pertama kali diberikan terhadap keberhasilan hiperimunisasi. Sebanyak 12 ekor induk sapi bunting jenis sapi Friesian Holstein, dibagi menjadi kelompok kontrol dan vaksin, dengan masing-masing kelompok terdiri dari umur kebuntingan enam bulan (n=3) dan tujuh bulan (n=3). Induk sapi diinjeksi vaksin Avian influenza (AI) H5N1 komersial (killed vaccine) sub-kutan, sebanyak 5 dosis/ekor (106 EID50/dosis). Vaksinasi dilakukan dua kali dengan interval antar vaksinasi selama dua minggu. Sebelum vaksinasi, induk sapi diberi imunomodulator 0,1 mg/kg bb per oral selama tiga hari berturut-turut kemudian dilakukan priming dengan menyuntikkan antigen AI H5N1 inaktif tanpa adjuvant (intravena) tiga hari berturut-turut (10x28 HAU/ekor). Sampel darah dikoleksi melalui vena coccygea sebelum vaksinasi pertama, dua minggu pascavaksinasi I dan dua minggu pascavaksinasi II untuk dianalisis terhadap parameter leukosit (jumlah total leukosit dan limfosit), kadar total protein, globulin dan rasio albumin/ globulin (A/G), serta titer IgG anti AI H5N1 dalam darah dan kolostrum. Hasil pengamatan menunjukkan tidak terdapat perbedaan yang signifikan pada jumlah total leukosit dan limfosit, kadar total protein dan globulin, rasio A/G, serta titer IgG anti AI H5N1 dalam darah dan kolostrum, baik antar kelompok maupun antar waktu pengamatan (p>0.05). Namun demikian, pada induk sapi kelompok vaksin umur kebuntingan enam bulan menunjukkan parameter leukosit, kadar total protein dan globulin serta titer IgG anti AI H5N1 dalam darah dan kolostrum lebih tinggi dibandingkan kelompok induk sapi umur kebuntingan tujuh bulan. Umur kebuntingan pada saat vaksinasi pertama kali dilakukan tidak memengaruhi keberhasilan hiperimunisasi pada induk sapi bunting trimester akhir.
The Kejadian Pertama Rabbit Haemorrhagic Disease Berdasarkan Studi Seroprevalensi di Provinsi Jawa Barat, Indonesia Retno Setyaningsih; I Wayan Teguh Wibawan; Surachmi Setiyaningsih; Ekowati Handharyani; Sri Murtini; Ahmad Biharidin
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.409

Abstract

Rabbit Haemmorhagic Disease (RHD) is one of viral diseases in rabbits that is still exotic in Indonesia. The RHD disease is caused by the Rabbit Haemorrhagic Disease Virus (RHDV) which is a calicivirus of the genus Lagovirus, Caliciviridae family. The high genetic variation of the RHDV and the rapid spread have the potential to disrupt rabbit farm (rabbittery) production and trade activities, especially rabbit exports, which require clarity on the status and situation of RHD disease in rabbits in Indonesia. A case of suspected RHD in Indonesia was first detected in the Philippines where rabbits exported from Indonesia were detected to be seropositive to RHD which resulted in rabbit export activities to the country was stopped. There is no data on the presence of RHD disease in rabbits in Indonesia, so the seroprevalence study is useful to provide preliminary information on the presence of this disease in Indonesia. This study was conducted on 163 rabbits samples raised in the Lembang area, Bandung, West Java, which is known as the largest rabbit farming center in Indonesia. Rabbit samples were taken at rabbit farms spread across seven villages namely Lembang, Pagerwangi, Cikahuripan, Cikole, Sukajaya, Gudangkahuripan and Jambudipa. The rabbits serum samples obtained were tested using the Indirect Enzym Linked Immunosorbant Assay (ELISA) method to determine the titer of antibodies against RHD. Based on the results of the analysis, it is known that 120 out of 163 rabbit serum samples showed positive antibody titers against RHD. The presence of antibody titers in rabbits on such farms can be preliminary information to be able to carry out further studies.
Co-Authors -, Mujiatun Abas, Farhat Abdul Kohar Abdul Zahid Adi Supryatno Adiningsih, Melani Wahyu Aep Saefullah Afiff , Usamah Agik Suprayogi Agung, Rizki Nugraha Agus Sutedjo Agus Sutejo Agus Suwondo Agus Wijaya Agustin, Riya Ahmad Biharidin Ahmad Furqon Ahmad Yusuf Aida Kurniawati Aisy, Izdihar Alen Ardiansyah Ali Ridho Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Almegi, Almegi Alzab, Amrie Muhammad Amrie Muhammad Andreas Erwin Prasetya Andrijanto Hauferson Angi Angelina N. Tethool Anggraini, Selviana Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Ansiska, Paisal Ardilasunu Wicaksono Arif Purwo Mihardi Asah Hilaliah Asep Surya Lesmana Ayi Santika Ayu Nurhania Ayundyayasti, Prima Bambang Hariyanto Bambang Sigit Widodo BAMBANG SIGIT WIDODO Bayu Febram Budi Prajogo Budiono, Novericko Ginger Cece Sumantri Christina, Budhi Adhiani Darma Jayanti, Viona Niscaya Dwi Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dewi, Arum Kusnila Diana, Fidia Nur Wahyu Dock, Chanthoeurn Dwi Hany Yanti Dwi Retno Wulandari Edi Sukmawinata Edi Sukmawinata Edward, Syafri Eka Maryam Eka Murtiasri EKO BUDIYANTO Eko Sugeng Pribadi Ekowati Handharyani Elok Budi Retnani Eprilianto, Deby Febrian Etih Sudarnika Fadirubun, Fahmi Fahrudin Fadjar Satrija Fadjar Satrija Fahmi Fahrudi Fadirubun Fahrudin Fadirubun, Fahmi Fajar Bayu Priyambodo Fathin, Khusnun Fitri Norhabiba, Fitri Fitria Susanti Fitria Susanti Fitriani, Hervina Gayatrie, Christina Retno Hasanudin, Mohammad Heirina, Anna HELMI -, HELMI Hendra Candra Hendriana, Okta Hilmy, Anggit Athoillah I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Ikhlasul, Galih Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Izdihar Aisy Jihan Rahmawati Joko Yunianto Prihatin Joned C. Saksana Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Ketut Prasetyo Khairunnisa, Poppy koekoeh santoso Kusaeri Kusaeri La Ode Wahidin Latifa, Rima Leli Anggreini Leni Maylina Lesmana, Asep Surya Lestari Sitohang, Lidya Lidya Lestari Sitohang Lismawati, Rina Listari, Tria Loso, Sugito Luci Cyrilla M.B.M Malole Made Agus Nurjana Makhmudiyah, Nurul Malonda Maksud Mansur, Ahmad Wafa Mardiana, Isa Mardinawati, Mardinawati Marlefzena Marlefzena Marliyati Marliyati, Marliyati Masrurah Masrurah Mawarseta, Sahra Melly Pratiwi Setyawati Melly Pratiwi Setyawati Miranti, Aliyah Muhammad Asrori Muhammad Fikri Al Habib Muhammad Noor Ardiansah Muharifah, Isnaini Mujiatun, Mujiatun Murtono Murtono Muzayanah Muzayanah Nanis Nurhidayah Natih, Ketut Karuni Nyanakumari Neksidin Neksidin, Neksidin Nensy Tri Putri Niken Ulupi Norma, Getrina Putri Noviriliensi Hartika Novita, Yeni Noviyanti Noviyanti Nugraha, Arifin Budiman Nugraha, Kenda Adhitya Nugrahaeni, Mutiara Nugraheni, Dyah Hapsari Eka Nugroho Hari Purnomo Nurhayati Nurhayati Nurly Faridah Oktandini Sugesti Putri Okti Nadia Poetri Opyn Mananta Pambudi, Slamet Pamungkas, Joko Perdan Prasetya, Sukma Perdana Prasetya , Sukma Permana, Rangga Pradika, Jaka Pranat, Gabriel Victor Yudha Prastiyono, Hendri Prima Ayundyayasti Purwaningsih Purwaningsih Putera, Alifi Restu Putra, Idrisi Raliya Putra, Raditya Pradana R Murwarni R. Susanti Raditya, Mochammad Nova Rafiq, Faizal Raharjani, Rani Rahmah, Ririn Ra'ifatur Ramadani Pardian Ramadini, Novia Ramlah Ramlah Rani Raharjanti Ratnawati, Diana Ravinesh Rohit Prasad Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Setyaningsih Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Riki Siswandi Rindawati Rindawati, Rindawati Risqika Akla Velayati Rita Mutia Riyansah, Anton Ronald Tarigan Rosyadi, Humam Rotinsulu, Dordia Anindita Rudi Afnan Rudiansyah RULLY ARDIANSYAH Ruvi, Muhammad Sabitah, Sabrina Azharia Sadida, Afiat Safitria Wulandari Salima, Rianti Samarang Samarang Santa Maria Lumbantoruan, Santa Maria Sarana Sarana Sari, Hanny Puspita Sari, Luis Purnama Satrija, Esdinawan Carakantara Saukhan, Ghiandra Naufal Syazily Sekar Ayu Chairunnisa Setiawan, Noor Rohman setya ningrum, mawar Silistyorini, Cahyo Ismawati Siregar, Fuad Siskamto Siti Gusti Ningrum, Siti Gusti Sitti Chadijah Sri Darwati Sri Estuningsih Sri Nuryati Sri Utaminingsih Sriviyantiningrum, Nurul Sujono Sujono, Sujono Sukarmin Sukarmin Sukma Perdana Prasetya Sulistinah Supriyanto Supriyanto Surachmi Setiyaningsih Suriadi Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Indri Nur Krisna TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tohiroh, Tohiroh Tria Lestari Triyono Triyono Ulfah Hidayati Ulfatin Khoiriyah Herowati Umi Faridah Upik Kesumawati Hadi Vajry, Indri Vianisa, Putri Virgiawan, Dhimas Bagus Wahono Esthi Prasetyaningtyas Wahyuni, Maria Eni Wirabumi, Putu Wiwien Mukti Andriyani Wiwien Mukti Andriyani WIWIK SRI UTAMI Wiwik Sri Utami Yananta, Ramadhan Andri Yani Sofiani YANUAR, ADY FITRA Yudhaningsih, Resi Yunitri, Ninik Yus Rusila Noor Yus Rusila Noor Yusuf Ridwan Zubair Arza