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All Journal HAYATI Journal of Biosciences Jurnal Rekayasa Proses VALENSI Geo Media: Majalah Ilmiah dan Informasi Kegeografian Jurnal Ilmu Dasar bionature Competitor : Jurnal Pendidikan Kepelatihan Olahraga Jurnal Sosialisasi: Jurnal Hasil Pemikiran, Penelitian dan Pengembangan Keilmuan Sosiologi Pendidikan E-Journal Widya Ekonomika Jurnal Kultur Demokrasi The Journal of Pure and Applied Chemistry Research Nursing News : Jurnal Ilmiah Keperawatan Jurnal Kimia Riset JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Pro Food Jurnal Kesehatan Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry Jurnal Pertanian Agros Jurnal Rekayasa Proses Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) JURNAL PENDIDIKAN TAMBUSAI Ensiklopedia of Journal Fullerene Journal of Chemistry COLLASE (Creative of Learning Students Elementary Education) PrimaryEdu - Journal of Primary Education JE (Journal of Empowerment) Jurnal Karinov Babul Ilmi Jurnal Ilmiah Multi Science Kesehatan Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Bisman (Bisnis dan Manajemen): The Journal of Business and Management Jurnal Ners Jurnal PORKES Indonesian Journal for Social Responsibility Jurnal Abdidas Jurnal Penelitian Pendidikan, Psikologi Dan Kesehatan (J-P3K) Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat International Journal of Digital Entrepreneurship and Business (IDEB) Journal of Community Development Jurnal Genre (Bahasa, Sastra, dan Pembelajarannya) ALACRITY : Journal Of Education Bulletin of Community Engagement BIOMA : Jurnal Biologi dan Pembelajarannya Journal of Current Health Sciences Journal of Educational Sciences Journal of Learning Improvement and Lesson Study Indonesian Sugar Research Journal Jurnal Ilmiah Ilmu Terapan Universitas Jambi Jurnal Profesi Pendidikan (JPP) Factory Jurnal Industri, Manajemen dan Rekayasa Sistem Industri Jurnal Pengabdian Masyarakat Biologi dan Sains West Science Business and Management MEUSEURAYA : JURNAL PENGABDIAN MASYARAKAT BAHASTRA AlBanna: Jurnal Pendidikan Islam Anak Usia Dini IJSS LIMNOTEK Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Jurnal Ilmu Hayat Bina Bangsa International Journal of Business and Management Jurnal Rekayasa Proses Society International Journal of Social Science and Community Service Al-Iqtishad: Jurnal Ekonomi Syariah Jurnal Lantera Ilmiah Kesehatan Jurnal Matematika Ilmiah Universitas Muhammadiyah Kuningan Abdurrauf Journal of Education and Islamic Studies Frontier Advances in Applied Science and Engineering Jurnal Kesehatan Masyarakat
Evi Susanti
Universitas Negeri Malang
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Kajian Potensi Limbah Pertanian Sebagai Sumber Karbon Pada Produksi Avicelase dan CMCase dari Bacillus circulans Amrullah Hamdan Rahmadani; Evi Susanti
Jurnal Kimia Valensi Jurnal Valensi Volume 3, No.2, November 2013
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.162 KB) | DOI: 10.15408/jkv.v3i2.503

Abstract

AbstrakAvicelase dan CMCase mewakili aktivitas eksoselulase dan endoselulase, termasuk dua dari tiga jenis selulase dalam sistem selulase yang dihasilkan oleh Bacillus circulans. Ekspresi keduanya sangat dipengaruhi oleh jumlah dan jenis sumber karbon dalam media produksi. Penelitian ini bertujuan mengetahui jenis dan konsentrasi limbah pertanian terbaik untuk menghasilkan avicelase dan CMCase dari B. circulans serta kemampuan sistem selulase tersebut untuk mendegradasi substrat lignoselulosa. Limbah pertanian yang diteliti berupa ampas tebu, bonggol jagung dan sekam. Limbah pertanian tersebut digunakan sebagai satu-satunya sumber karbon pada media produksi. Avicel digunakan sebagai sumber karbon pembanding. Aktivitas avicelase dan CMCase ditentukan berdasar jumlah gula pereduksi yang dihasilkan dengan menggunakan metoda Somogy-Nelson. Hasil penelitian menunjukkan bahwa semua limbah pertanian yang diteliti berpotensi sebagai sumber karbon yang lebih baik dibandingkan dengan avicel untuk produksi avicelase dan CMCase dari B. circulans. Limbah pertanian yang terbaik adalah ampas tebu. Penggunaan ampas tebu pada konsentrasi 0,5 % menghasilkan aviselase dan CMCase sebesar 309,39 dan 405,48 U/mL. Sistem selulase yang dihasilkan mampu mendegradasi ampas tebu, bonggol jagung dan sekam.Kata kunci : avicelase, CMCase, limbah pertanian, sumber kabon, Bacillus circulansAbstractAvicelase and CMCase activity represent exocellulase and endocellulase, including two of the three types of cellulase in the cellulase system produced by Bacillus circulans. Expression both of them strongly influenced by the amount and type of carbon source in the production medium. The aims of this research were to determine the type and concentration of the best agricultural waste to generate avicelase and CMCase from B. circulans and their ability to degrade lignocellulosic substrates. Studied agricultural wastes are bagasse, corn stalks and husks. Agricultural waste is used as the sole carbon source in media production. Avicel is used as a comparable carbon source. Avicel and CMCase activity are determined based on the amount of the reducing sugars is generated by using the method of Somogy - Nelson. The results showed that all the studied agricultural waste was a potential carbon source better than ethical for avicelase and CMCase production from B. circulans. Agricultural waste bagasse was the best one. The use of 0.5 % bagasse produce CMCase and aviselase at 309.39 and 405.48 U / mL respectively. The resulting system was able to degrade bagasse , corn stalks and husks.Keywords : avicelase , CMCase , agricultural waste , carbon sources , Bacillus circulans
Ligninase Profiling and Optimization of Laccase Production from Indigenous Wood Rot Fungus (WRF) KLUM2 in Kirk Medium-Alkali Lignin Kayu Jati (MK-ALKJ) Siti Mutmainah; Evi Susanti
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 7, No. 1, May 2021
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v7i1.20895

Abstract

The production of ligninase by wood rot fungus (WRF) is determined by carbon source and growth condition. The goal of this study is to determine the ligninase profile produced by WRF KLUM2 in Kirk Medium using teak wood alkaline lignin as a carbon source known as Kirk Medium-Alkali lignin Kayu Jati (MK-ALKJ), optimization of dominant ligninase production in the MK-ALKJ compared to the one that is produced in the Kirk’s medium with glucose as a carbon source (MK-Glucose). This research was conducted in an experimental laboratory consisting of: (1) spore suspension preparation, (2) ligninase profiling at various growth times, (3) ligninase profiling at various temperature variations, (4) optimization of laccase production including pH and the amount of nitrogen source. Growth was identified based on the specific activity of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase. The results showed that relatively the three types of ligninase, namely LiP, MnP, and laccase, were produced in the same amount by the wood rotting fungus isolates KLUM2 in MK-ALJK. All three were produced with the highest yield of respectively 55.65; 52.48; 57.64 U/mg. Laccase as the dominant ligninase can be optimized to reach 83.52 U/mg by inoculating 2.107 spore cells in MK-ALKJ in 37 °C, pH = 3.5, and a nitrogen source of 20mM (NH4)2SO4 for 6 days. Therefore, it can be concluded that the ligninase activity of indigenous WRF KLUM2 in MK-ALJK medium is higher than in the MK-Glucose.
Exploration The Candidates of Xenobiotic Degrading Indigenous Bacteria from Probolinggo City Landfill by Using Next Generation Sequencing (NGS) Qodriyah, Nur Romadhona Lailatul; Sanjaya, Eli Hendrik; Wahab, Roswanira Abdul; Susanti, Evi
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 9, No. 2, November 2023
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v9i2.34316

Abstract

Soil bacteria from tropical environments play a significant role in resolving various environmental issues, including biodegradation. Exploratory research on biodiversity is crucial to develop and harness the potential of different types of soil bacteria that are highly abundant. The bacterial diversity in landfills is typically high due to the decomposition of organic and inorganic waste, creating a favorable medium for the growth and development of soil bacteria. This study aims to assess the candidates of xenobiotic degrading indigenous bacteria from the Probolinggo City landfill using Next Generation Sequencing (NGS) method. The research stages include: 1) sampling, 2) isolation of genomic DNA from samples using the ZymoBIOMICS DNA MiniPrep Kit from Zymo Research, 3) amplification of isolated DNA with primers 16S 27F – 1429R, 4) sequencing the results of DNA amplification with NGS, 5) downstream analysis of the results using software Pavian Krona Tools, and 6) narrative analysis review to identify the candidates of xenobiotic degrading indigenous bacteria. The results show that soil samples from the Probolinggo City landfill exhibited a high diversity of bacterial communities. Based on NGS analysis, 2400 bacterial species were identified, comprising 56 genera, 17 orders, 4 classes, and 4 phyla, with respective abundances of Proteobacteria (70%), Firmicutes (15%), Planctomycetes (2%), and Cyanobacteria (0,3%). Based on the narrative analysis review, several bacteria in the Probolinggo City landfill exhibited potential as: 1) polypropylene-degrading bacteria, including Bacillus cereus, B. licheniformis and B. thuringiensis. 2) styrofoam degrading bacteria, namely Bacillus amyloliquefaciens, Bacillus cereus, Bacillus firmus and Pseudomonas aeruginosa. 3) total ammonia nitrogen (TAN) reducing bacteria, including Bacillus megaterium. 4) pesticide degrading bacteria Profenofos and Chlorantraniliprole, including Bacillus stearothermophilus. and 5) tannic acid degrading bacteria, including Pantoea dispersa. These results indicate that the Probolinggo City landfill is a good habitat for various xenobiotic-degrading bacteria, then the isolation of specific bacteria can be designed using an appropriate selective medium.
Decolorization of Reactive Black 5 by Myceliophthora thermophila KLUM1 Susanti, Evi; Jati, Rizky Febriani; Apriani, Dwi; Suharti, Suharti; Alvionita, Mieke
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.45573

Abstract

This research aimed to ascertain the impact of alkaline lignin of teak sawdust on the synthesis of ligninase and decolorization of Reactive Black 5 (RB5) by M. thermophilia KLUM1, also to elucidate the mechanism of mycoremediation. This research was conducted within the cultivation of the spore suspension in a co-culture setting. The RB5 dye percentage decolorization and ligninase activity were produced by varying the addition of alkaline lignin. The results show that the addition of 0.5% alkaline lignin accelerates the production of ligninase in the Kirk medium. However, adding more than 0.5% alkaline lignin does not affect the highest amount of ligninase produced. The increase in RB5 dye decolorization by M. thermophila KLUM1 is proportional to the increase in alkaline lignin in the medium. The percentage of wood-rot fungi decolorization in Kirk medium with and without lignin with the addition of 0.5%, 1%, and 2% lignin on the 10th day was 26.29%, 29.51%, 34.74%, and 40.43%, respectively. The decolorization process of RB5 by M. thermophilia KLUM1 in Kirk medium with alkaline lignin occurs through several mechanisms, i.e., enzymatic degradation, adsorption by the growing mycelia, and alkaline lignin present in the medium.
Co-Authors Abyan Farras Putra ADE HIKMAT Adelina Hasyim agrita, tri wera Agung Winarno Agus Waluyo Ahmad Jamalong, Ahmad Ahmad, M. Ridwan Said Aiman Ibrahim Alifah, Naila Nur Alvionita, Mieke Amir, Widya Pasca Amrullah Hamdan Rahmadani Andi Aco Agus, Andi Aco Andri Prasetiyo Annasa Sabatia Arifin, Ardian Arifin, Fawaid Syamsul Aris Aby Chisna Ariyanto, Irvan Artasasta, Muhammad Ade Artikawati, Mada Shahar  Aryani, Desy Assaajidah, Alima Aulanni'am Aulanni'am Ayu Nurdiyan Bunda Halang Chrismadha, Tjandra Cici Apriza , Yanti Citra Utami Debby Ratno Kustanto, Debby Delia Wahyu Pangesti Delia Wahyu Pangesti Dewi Puspitasari Dhani, Muhammad Dian Oktaviyani Dian Sari DIMYATI, AKHMAD Dita, Rahma Djamarah, Syaiful Bahri Dwi Apriani Dwi Listyorini Dyati Galuh Pratita Eka Prihatinningtyas Elfira Husna, Elfira Elhah Nailul Khasna Eli Hendrik Sanjaya Erian Fatria Esti Mumpuni, Esti Esti Nanda Viatari Etik M. Achadian Etty Soesilowati Fadia Zen Fadila Abna Swari Fakhren Nukha Zalfa Fauzi Ashra Fawaid Syamsul Arifin Fikri Munif Nashrullah Fredia Heppy Fredia Heppy, Fredia Gandi, Putri Indra Ginting, Dafid Hadianti, Yeni Hagmy Nurma Yolandasari Hanifa, Syafira Haq, Ilham Abdul Harisnal Hartati Eko Wardani Hayati, Iznirrahma Hellen Febriyanti Herdiana, Dian Heri Pratikto Hermi Yanzi Hidayati Hidayati Hidayati, Arifah Hidayati, Nanda Hikmah Tri Prihatini Hilal Mulyadi I Gusti Ayu Agung Pradnya Paramita Ike Astiyandani Ikhsan Ikhsan Ikhsan Ikhsan Ikram, Muhammad Haykal Ilham Dwi Susilo Widiatmoko Indah Zahra Indra Kurniawan Saputra Indra Setiawan Indra Setiawan Intan Chairun Nisa Iqbal Achmad Sabilla Irwami, Astri Ayu Ismanto, - Ismet Isthi Wahyuning Tyas Jati, Rizky Febriani Jovan Hilmansyah Rantepadang Juniar, Risky Tri Junios Junios Ketang Wiyono Khairuddin Hasan Krisnanda, Isma Riyan Kukuh Nirmala Kurniawan, Riky Lidia, Siska Lubis, Zurlina Luthfiyah, Ummu M IRFAN, M Madziatul Churiyah Mahbub Romdlon Muhammad Mahesya Surya Saputra Ramadhan Mahmudah, Ika Zulkafika Mailisa, Riski Manuardi, Ardian Renata Mardiana Lelitawati Marjuni Marjuni Marzuki , Marzuki Mawijaya, Agus Mayangsari, Nadia Erlina Melisa Surya Andini Mellia Fransiska, Mellia Mochammad Imron Awalludin Monika Rahayu Mufti Hatur Rahmah Muh Ade Artasasta Muh. Rizal Kurniawan Yunus Muhammad Ali Murtadho Muhammad Mujtaba Habibi Muhammad Naufal Sakha Nararya Muhammad Reza Pahlevi Muhammad Rivai Muhammad Suhaemi Syawal Muhammad Zahran Edlian Syach Muharam, Nyi Raden Siti Vissy Puspa Dewi Muhimmatul Aliyah Muit, Nasrul Mulatsari, Esti Musayyada Nadia Erlina Mayangsari Nadiya Eka Suryani Nafisah, Ifa Nafisyah, Eva Naila Nur Alifah Najla Aulia Arief Nani Farida Nararya, Muhammad Naufal Sakha Nasrul, Muit Nia Lutfiana Lutfiana Ningrum, Mardani Eka Nofirda, Fitri Ayu Norazlinaliza Salim Norman Yoshi Haryono Nova Herawati Novita Oktavianti Parera Nur Rahmawati Nur' Aisha Nurdin Nurdin Nurfaidah, Nadia Nurhasanah, Yeni Nurhaslita Sari NURUL KHOTIMAH Oktaviyani, Dian Pingkan Sihasaleh Pratiwi Lestari Pretty Ferdiyanti Prihatini, Hikmah Tri Puji Hastuti, Yuni Puput Safitri Wijaya Putri Meilani Qodriyah, Nur Romadhona Lailatul Ragil Catur Adi W. Rahayu, Riska Agni Rahma, Fika Aulia Rahman, Sari Rahayu Rahmulyana, Anjar Ramadanti, Indah Putri Ramadhan Firdaus, Anugrah Ratna Juwita Rebecca Hellen Wikarjanto Retno Wulandari Retno Wulandari Rezi Fahleva Rhahmadini, Yahdi Thia Ridho, Tri Ibnu Rina Anindita Rina Rifqie Mariana Ristanti Widoretno, Mey Rizqi Layli Khusufi Rohilian Wisald Roiyul Mufidah Ronny Mugara, Ronny Rosini, Iin Rulfia Rulfia Desi Maria, Rulfia Ryuzen, Fahrizal Sabda Wahab Safirah Ramadhaniati Salsabila Kasta Hygiea Iswara Salsabila, Salsabila Sanjaya, Riona Saputra, Andika Dimas Saputra, Hijrah Sari, Nurhaslita Sarif Hidayat Sartika, Nila Satya, Awalina septia, qori armiza Setiyadi, Ruli Shinta Wulandari, Shinta Shinta Yuliana Sholikan Sholikan Simanjuntak, Josua Donrio Sindy Oktari Putri Sinta Yuliana Siti Khoirunnisa Siti Khoirunnisa, Siti Siti Mutmainah Siti Zulaiha Soasa, Cindy Sovia, Adlin Dewi Subroto, Desty Endrawati Sugiarti SUGIYANTO Suharjono Suharjono Suharti Suharti Sumari Sumari Sumayyah Sumayyah, Sumayyah Sumi Lestari, Fajar Sunarmi Suryandini, Hedmy Keisyakhanti Suwandi, Febrian Rahmat Suwanto, Witri Syafi'i, Imam Syafira Maharani Syahrani, Annisa Syarif , Maulana Tanto Hariyanto Taqwa, Iqbal Ananda Tita Putri Milasari Titis Wulandari Tri Ardyati Tri Astuti Trisna Nugraha Tuti Oktriani Utami, Mutiara Viona, Aliah Vira Yunidar Virginia Kawer, Moudy Wahab, Roswanira Abdul Waldi Fajar Ramadhan Wel Welni Wel Welni, Wel Wicaksono, Dyandra Maharani Putri Widiyati, Dian Wijayanti, Chandra Wini Tarmini Wira Eka Putra Wiwaha, Arjuna Wiwik WIWIK Wizia, Lady Yade Kurnia Sari Yanuar Rohmat Aji Yayuk Mulyati Yeltra Armi, Yeltra Yessy Triana Dewi Yoko Masnarivan, Yoko Yon Ade Lose Yona Desni Sagita, Yona Desni Yovi Pranata Yudi Abdul Majid Yuhendri Putra Yuli Agustina, Yuli YULIANA, SHINTA Zahroh, Vanni Fatimah Zalfa, Fakhren Nukha Zarin Nafasari Zenn, Arina Putri Zidan Aqilah Rasyid Zoni, Henri