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EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi Sekar Ayu Chairunnisa; Sri Nuryati; Alimuddin Alimuddin; Sri Murtini; Ayi Santika; Dwi Hany Yanti
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (54.329 KB) | DOI: 10.15578/iaj.11.1.2016.31-39

Abstract

Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and  positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.
EKSPRESI PROTEIN COAT DAN mRNA VIRAL NERVOUS NECROSIS YANG DIKENDALIKAN OLEH PROMOTER β-AKTIN IKAN MEDAKA DAN KERATIN IKAN FLOUNDER JEPANG Wiwien Mukti Andriyani; Sri Murtini; Alimuddin Alimuddin; I.W. Teguh Wibawan
Jurnal Riset Akuakultur Vol 9, No 1 (2014): (April 2014)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.608 KB) | DOI: 10.15578/jra.9.1.2014.79-86

Abstract

Kemampuan promoter dalam mengatur ekspresi gen penyandi protein imunogenik sangat menentukan efikasi suatu vaksin DNA. Penelitian ini bertujuan untuk mengukur tingkat ekspresi protein dan mRNA RNA2 penyandi coat protein (CP) virus viral nervous necrosis (VNN) yang dikendalikan oleh dua promoter berbeda, yaitu promoter β-aktin ikan medaka (mBA), dan keratin ikan flounder Jepang (JfKer). Uji ekspresi CP dilakukan menggunakan embrio ikan lele dumbo (Clarias sp.) sebagai model, sedangkan analisis mRNA dilakukan menggunakan ikan kerapu tikus. Konstruksi vektor ekspresi pmBA-CP dan pJKer-CP dengan konsentrasi 50 ng/μL KCl 1 M disuntikkan ke embrio ikan lele dumbo fase 1-2 sel. Sebanyak 30 embrio ikan lele dumbo diambil pada jam ke-6, 8, 10, 12, 14, dan 16 pascainjeksi untuk analisis protein. Hasil SDS-PAGE menunjukkan adanya protein berukuran sekitar 42 kDa, dan analisis western blot menggunakan antibodi (Ab) poliklonal anti-VNN membuktikan bahwa protein tersebut adalah CP. Keberhasilan deteksi protein spesifik menggunakan Ab anti-VNN tersebut menunjukkan bahwa embrio ikan lele dapat digunakan untuk menguji potensi produksi protein imunogenik yang dikendalikan oleh promoter berbeda. Pengujian ini juga menunjukkan bahwa, aktivitas promoter mBA lebih tinggi daripada promoter JfKer, sehingga uji ekspresi mRNA dilakukan menggunakan konstruksi pmBA-CP. Benih ikan kerapu tikus (panjang badan sekitar 5 cm) diinjeksi dengan pmBA-CP secara intramuskular dengan dosis 12,5 μg/ekor. Total RNA diekstraksi dari daging pada waktu 6, 12, dan 24 jam pascainjeksi. Hasil RT-PCR menunjukkan adanya ekspresi mRNA CP pada 24 jam pascainjeksi. Hal tersebut menunjukkan bahwa promotor mBA aktif mengendalikan ekspresi CP pada ikan kerapu tikus, dan pmBA-CP berpotensi digunakan sebagai vaksin DNA untuk menginduksi kekebalan ikan kerapu terhadap infeksi VNN.
Profil hematologi anjing lokal di wilayah endemik rabies Agus Wijaya; Ronald Tarigan; Koekoeh Santoso; Yusuf Ridwan; Etih Sudarnika; Abdul Zahid Ilyas; Denny Widaya Lukman; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Sri Murtini; Edi Sukmawinata; Annisa Madyanti Geminastiti Parampasi
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.224 KB) | DOI: 10.29244/avl.2.1.1-2

Abstract

Provinsi Jawa Barat merupakan satu dari 24 provinsi di Indonesia yang dilaporkan belum bebas Rabies. Usaha pembebasan wilayah Jawa Barat dari penyakit Rabies sangat strategis mengingat posisi Jawa Barat berada di antara daerah bebas Rabies. Jampang Tengah menjadi salah satu kecamatan di Sukabumi yang pada tahun 2016 dilaporkan telah terjadi 13 kasus gigitan Rabies. Penelitian ini menggunakan 81 sampel darah anjing lokal yang dikoleksi dari enam desa di kecamatan Jampang Tengah dan diperiksa dengan menggunakan alat Rayto® hematology analyzer. Parameter profil hematologi dikelompokkan berdasarkan perbedaan umur dan jenis kelamin anjing. Hasil pemeriksaan hematologi secara umum berdasarkan umur menunjukkan nilai rataan yang lebih besar pada anjing lokal umur ≥1 tahun dan konsentrasi leukosit menunjukkan nilai statistik yang signifikan berbeda (p<0.05). Berdasarkan jenis kelamin, nilai rataan parameter hematologi secara umum lebih tinggi pada anjing lokal betina dan adanya perbedaan signifikan (p<0.05) pada konsentrasi leukosit dan granulosit.
Keberhasilan Hiperimunisasi Pada Sapi Bunting: Kajian Terhadap Ketepatan Waktu Vaksinasi Safitria Wulandari; Anita Esfandiari; Sri Murtini; Retno Wulansari
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.565 KB) | DOI: 10.19087/jveteriner.2022.23.1.88

Abstract

Penelitian ini bertujuan untuk melihat pengaruh umur kebuntingan induk sapi saat vaksin pertama kali diberikan terhadap keberhasilan hiperimunisasi. Sebanyak 12 ekor induk sapi bunting jenis sapi Friesian Holstein, dibagi menjadi kelompok kontrol dan vaksin, dengan masing-masing kelompok terdiri dari umur kebuntingan enam bulan (n=3) dan tujuh bulan (n=3). Induk sapi diinjeksi vaksin Avian influenza (AI) H5N1 komersial (killed vaccine) sub-kutan, sebanyak 5 dosis/ekor (106 EID50/dosis). Vaksinasi dilakukan dua kali dengan interval antar vaksinasi selama dua minggu. Sebelum vaksinasi, induk sapi diberi imunomodulator 0,1 mg/kg bb per oral selama tiga hari berturut-turut kemudian dilakukan priming dengan menyuntikkan antigen AI H5N1 inaktif tanpa adjuvant (intravena) tiga hari berturut-turut (10x28 HAU/ekor). Sampel darah dikoleksi melalui vena coccygea sebelum vaksinasi pertama, dua minggu pascavaksinasi I dan dua minggu pascavaksinasi II untuk dianalisis terhadap parameter leukosit (jumlah total leukosit dan limfosit), kadar total protein, globulin dan rasio albumin/ globulin (A/G), serta titer IgG anti AI H5N1 dalam darah dan kolostrum. Hasil pengamatan menunjukkan tidak terdapat perbedaan yang signifikan pada jumlah total leukosit dan limfosit, kadar total protein dan globulin, rasio A/G, serta titer IgG anti AI H5N1 dalam darah dan kolostrum, baik antar kelompok maupun antar waktu pengamatan (p>0.05). Namun demikian, pada induk sapi kelompok vaksin umur kebuntingan enam bulan menunjukkan parameter leukosit, kadar total protein dan globulin serta titer IgG anti AI H5N1 dalam darah dan kolostrum lebih tinggi dibandingkan kelompok induk sapi umur kebuntingan tujuh bulan. Umur kebuntingan pada saat vaksinasi pertama kali dilakukan tidak memengaruhi keberhasilan hiperimunisasi pada induk sapi bunting trimester akhir.
Effication of Viral Nervous Necrosis DNA as Vaccines for Cromileptes altivelis Wiwien Mukti Andriyani; Sri Murtini; Alimuddin Alimuddin
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 3 No. 2 (2020): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v3i2.13891

Abstract

Viral nervous necrosis (VNN) is a disease that often infects groupers. It has caused mass death in more than 34 species of marine fish. DNA vaccination might become a solution againts the infection. The construction of the pmBA-CP DNA vaccine consisting of the beta-actin promoter of medaka fish (Oryzias latipes) and capsid protein (CP) encoding VNN RNA2 has been made in previous studies. This study aimed to test the efficacy of the pmBA-CP DNA vaccine for VNN. The experiment consisted of two stages, namely (1) detection of anti-VNN antibody induction in vaccinated fish using ELISA, and (2) challenge test for fish vaccinated with the VNN virus. Grouper (body length 8 cm to 10 cm) were divided into two groups with a density of 5 fish 60 L–1. The fish in the first group were vaccinated with pmBA-CP intramuscularly at a dose of 12.5 µg per fish, while the second group of fish were not vaccinated. Antibody titer testing was carried out before treatment, and 1 d, 7 d, 14 d, 21 d, 28 d, and 35 d after vaccination. The challenge test was carried out on the 60th day after vaccination. The results showed that the S / P ratio in the vaccinated fish serum was higher than unvaccinated fish at 21 d to 35 d post-vaccination. DNA vaccination was able to induce anti-VNN antibodies of grouper. The results of the challenge test for vaccinated fish using VNN virus titer 103.5 FID50/0.2 mL showed 60% of survival rate. Thus, the pmBA-CP DNA vaccine could be useful for increasing grouper immunity, and support production of grouper.
Polymorphisms of the Thy1 (Thymocyte Differentiation Antigen 1) Gene in Candidate IPB D2 Chicken Line Nensy Tri Putri; Sri Murtini; Niken Ulupi; Isyana Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.3029

Abstract

The Thy1 gene is a member of the immunoglobulin superfamily, which is found in many different animals. This gene encodes a Thy1 protein (CD90) which measures 25–37 kDa. Although the function of the Thy1 gene is unknown, it is thought to play a crucial role in the immunological response, particularly in the activation of T cells. The goal of this study was to find a variation in the Thy1 gene in IPB D2  chickens that might be utilized as candidates for immunoresistance genetic markers. A total of 123 samples from IPB D2 chickens consisting of 38 samples from IPB D2 G0 (basic generation) chickens and 85 samples from IPB D2 G2 (second generation) chickens were used in this study. PCR methods and direct-DNA sequencing were used in this study. Data analysis in this study consisted of genotype frequency, allele frequency, observed heterozigosity, expected heterozygosity, and Hardy-Weinberg equilibrium. The results of the study  discovered two SNPs on the intron (g.2139 CT and g.2542 AG). Four haplotypes were created by combining two SNPs. The g.2139 CTand g.2542 AG were polymorphic, but not in the Hardy-Weinberg equilibrium.
Mammalian Contribution to Transmission of Schistosoma japonicum Infection in West Lore, Poso, Central Sulawesi, Indonesia Novericko Ginger Budiono; Fadjar Satrija; Yusuf Ridwan; Ekowati Handharyani; Sri Murtini; Opyn Mananta
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 3 (2022): September 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i3.3026

Abstract

Studies on the role of domestic animals in the transmission of schistosomiasis japonica in the West Lore Sub-district, Poso District, are still limited despite its importance as zoonosis. This study used a cross-sectional design to determine schistosomiasis prevalence in domestic mammals and identify the relative contribution of each mammalian species’ schistosomiasis transmission in the West Lore Sub-District. Fecal samples were obtained from 209 animals (seven buffaloes, 70 dogs, 44 cattle, 86 pigs, and two horses). The Danish Bilharziasis Laboratory technique was used to detect both the occurrence of S. japonicum egg in feces and the intensity of schistosomiasis infection. The examination of 1852 human fecal samples using the Kato-Katz method was carried out by the Laboratory of Schistosomiasis Lengkeka. The measurement of environmental pollution with S. japonicum eggs and the relative contribution of each species in the transmission was performed. The highest prevalence of S. japonicum infection in animals was in horses (100%; 2/2), cattle (54.55%; 24/44), and pigs (51.16%; 44/86). The prevalence in buffaloes and dogs was 28.57% (2/7) and 32.86% (23/70). Cattle (69.74%) were the main contributors to S. japonicum eggs contamination in the environment, followed by pigs (21.95%) and buffaloes (4.71%). This study reported a high prevalence of schistosomiasis in animals (45.46%), while low human schistosomiasis prevalence (0.59%).
The Kejadian Pertama Rabbit Haemorrhagic Disease Berdasarkan Studi Seroprevalensi di Provinsi Jawa Barat, Indonesia Retno Setyaningsih; I Wayan Teguh Wibawan; Surachmi Setiyaningsih; Ekowati Handharyani; Sri Murtini; Ahmad Biharidin
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.409

Abstract

Rabbit Haemmorhagic Disease (RHD) is one of viral diseases in rabbits that is still exotic in Indonesia. The RHD disease is caused by the Rabbit Haemorrhagic Disease Virus (RHDV) which is a calicivirus of the genus Lagovirus, Caliciviridae family. The high genetic variation of the RHDV and the rapid spread have the potential to disrupt rabbit farm (rabbittery) production and trade activities, especially rabbit exports, which require clarity on the status and situation of RHD disease in rabbits in Indonesia. A case of suspected RHD in Indonesia was first detected in the Philippines where rabbits exported from Indonesia were detected to be seropositive to RHD which resulted in rabbit export activities to the country was stopped. There is no data on the presence of RHD disease in rabbits in Indonesia, so the seroprevalence study is useful to provide preliminary information on the presence of this disease in Indonesia. This study was conducted on 163 rabbits samples raised in the Lembang area, Bandung, West Java, which is known as the largest rabbit farming center in Indonesia. Rabbit samples were taken at rabbit farms spread across seven villages namely Lembang, Pagerwangi, Cikahuripan, Cikole, Sukajaya, Gudangkahuripan and Jambudipa. The rabbits serum samples obtained were tested using the Indirect Enzym Linked Immunosorbant Assay (ELISA) method to determine the titer of antibodies against RHD. Based on the results of the analysis, it is known that 120 out of 163 rabbit serum samples showed positive antibody titers against RHD. The presence of antibody titers in rabbits on such farms can be preliminary information to be able to carry out further studies.
Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mxgene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.
SEROSURVEILLANCE OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 WITH HAEMAGGLUTINATION-INHIBITION ON WILD AQUATIC BIRDS IN PULAU DUA SERANG NATURAL RESERVES, BANTEN PROVINCE Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri; Noor, Yus Rusila
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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Abstract

Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.
Co-Authors -, Mujiatun Abas, Farhat Abdul Kohar Abdul Zahid Adi Supryatno Adiningsih, Melani Wahyu Aep Saefullah Afiff , Usamah Agik Suprayogi Agung, Rizki Nugraha Agus Sutedjo Agus Sutejo Agus Suwondo Agus Wijaya Agustin, Riya Ahmad Biharidin Ahmad Furqon Ahmad Yusuf Aida Kurniawati Aisy, Izdihar Alen Ardiansyah Ali Ridho Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Almegi, Almegi Alzab, Amrie Muhammad Amrie Muhammad Andreas Erwin Prasetya Andrijanto Hauferson Angi Angelina N. Tethool Anggraini, Selviana Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Arif Purwo Mihardi Asah Hilaliah Asep Surya Lesmana Ayi Santika Ayu Nurhania Ayundyayasti, Prima Bambang Hariyanto BAMBANG SIGIT WIDODO Bambang Sigit Widodo Bayu Febram Budi Prajogo Budiono, Novericko Ginger Cece Sumantri Christina, Budhi Adhiani Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dewi, Arum Kusnila Dock, Chanthoeurn Dwi Hany Yanti Dwi Retno Wulandari Edi Sukmawinata Edi Sukmawinata Edward, Syafri Eka Maryam Eka Murtiasri EKO BUDIYANTO Eko Budiyanto Eko Sugeng Pribadi Ekowati Handharyani Elok Budi Retnani Eprilianto, Deby Febrian Etih Sudarnika Fadirubun, Fahmi Fahrudin Fadjar Satrija Fadjar Satrija Fahmi Fahrudi Fadirubun Fahrudin Fadirubun, Fahmi Fathin, Khusnun Fitri Norhabiba, Fitri Fitria Susanti Fitria Susanti Fitriani, Hervina Gayatrie, Christina Retno Hasanudin, Mohammad Heirina, Anna HELMI -, HELMI Hendra Candra Hendriana, Okta Hilmy, Anggit Athoillah I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Ikhlasul, Galih Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Izdihar Aisy Jihan Rahmawati Joko Yunianto Prihatin Joned C. Saksana Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Ketut Prasetyo koekoeh santoso Kusaeri Kusaeri La Ode Wahidin Latifa, Rima Leli Anggreini Leni Maylina Lesmana, Asep Surya Lestari Sitohang, Lidya Lidya Lestari Sitohang Lismawati, Rina Listari, Tria Loso, Sugito Luci Cyrilla M.B.M Malole Made Agus Nurjana Makhmudiyah, Nurul Malonda Maksud Mansur, Ahmad Wafa Mardinawati, Mardinawati Marlefzena Marlefzena Marliyati Marliyati, Marliyati Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Asrori Muhammad Fikri Al Habib Muhammad Noor Ardiansah Muharifah, Isnaini Mujiatun, Mujiatun Murtono Murtono Nanis Nurhidayah Natih, Ketut Karuni Nyanakumari Neksidin Neksidin, Neksidin Nensy Tri Putri Niken Ulupi Norma, Getrina Putri Noviriliensi Hartika Novita, Yeni Noviyanti Noviyanti Nugraha, Arifin Budiman Nugraha, Kenda Adhitya Nugrahaeni, Mutiara Nugraheni, Dyah Hapsari Eka Nugroho Hari Purnomo Nurhayati Nurhayati Nurly Faridah Oktandini Sugesti Putri Okti Nadia Poetri Opyn Mananta Pambudi, Slamet Pamungkas, Joko Perdan Prasetya, Sukma Permana, Rangga Pradika, Jaka Prastiyono, Hendri Prima Ayundyayasti Purwaningsih Purwaningsih Putera, Alifi Restu Putra, Idrisi Raliya Putra, Raditya Pradana R Murwarni R. Susanti Raditya, Mochammad Nova Rafiq, Faizal Raharjani, Rani Rahmah, Ririn Ra'ifatur Ramadani Pardian Ramlah Ramlah Rani Raharjanti Ratnawati, Diana Ravinesh Rohit Prasad Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Setyaningsih Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Riki Siswandi Rindawati, Rindawati Risqika Akla Velayati Rita Mutia Ronald Tarigan Rosyadi, Humam Rotinsulu, Dordia Anindita Rudi Afnan Rudiansyah Rudiansyah, Rudiansyah RULLY ARDIANSYAH Ruvi, Muhammad Sabitah, Sabrina Azharia Sadida, Afiat Safitria Wulandari Salima, Rianti Samarang Samarang Santa Maria Lumbantoruan, Santa Maria Sarana Sarana Satrija, Esdinawan Carakantara Saukhan, Ghiandra Naufal Syazily Sekar Ayu Chairunnisa Setiawan, Noor Rohman Silistyorini, Cahyo Ismawati Siregar, Fuad Siskamto Siti Gusti Ningrum, Siti Gusti Sitti Chadijah Sri Darwati Sri Estuningsih Sri Nuryati Sri Utaminingsih Sriviyantiningrum, Nurul Sukma Perdana Prasetya Sulistinah Supriyanto Supriyanto Surachmi Setiyaningsih Suriadi Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Indri Nur Krisna TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tohiroh, Tohiroh Tria Lestari Triyono Triyono Ulfah Hidayati Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Vianisa, Putri Virgiawan, Dhimas Bagus Wahono Esthi Prasetyaningtyas Wahyuni, Maria Eni Wirabumi, Putu Wiwien Mukti Andriyani Wiwien Mukti Andriyani WIWIK SRI UTAMI Wiwik Sri Utami Yani Sofiani YANUAR, ADY FITRA Yudhaningsih, Resi Yunitri, Ninik Yus Rusila Noor Yus Rusila Noor Yusuf Ridwan Zubair Arza