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Pengaruh Metode Ekstraksi Terhadap Kandungan Senyawa Polifenol dan Aktivitas Antioksidan pada Rubus fraxinifolius Yesi Desmiaty; Berna Elya; Fadlina Chany Saputri; Iis Irawatty Dewi; Muhammad Hanafi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 17 No 2 (2019): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.654 KB) | DOI: 10.35814/jifi.v17i2.755

Abstract

Rubus fraxinifolius can be founded in the mountainous regions of West Java. Previous studies have shown that this plant has potent antioxidant activity. The content of polyphenol compounds in this species contributes substantially to antioxidant activity, and the extraction method will affect the content of the compound. In this study an evaluation of the extraction method effect on leaves, young fruits and stems of R. fraxinifolius on the content of polyphenols and their antioxidant activity, have been done. Method: The leaves, fruits, and stems of R. fraxinifolius were extracted by maceration, reflux, and soxhlet using methanol as a solvent. Each extract was determined the total polyphenol content using Folin-Ciocalteu reagent and antioxidant activity test using DPPH reduction method. Result: The highest extract yield was R. fraxinifolius leaf reflux extract (30.20%). The highest total polyphenol content was given by the Soxhlet extract of leaf R. fraxinifolius (48.79 mg GAE / g extract). The highest antioxidant activity was leaf Soxhlet extract of R. fraxinifolius (98.29% at 100 ppm). Conclusion: From this study it was shown that the methanol extract of R. fraxinifolius leaves extracted by Soxhlet gave the highest polyphenol content and antioxidant activity.
ELUSIDASI STRUKTUR MOLEKUL UK-3, UK-4 DAN UK-5, SENYAWA BIOAKTIF DARI Streptomyces sp. 517-02. Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 8, No 1-2 (1998)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v8i1-2.204

Abstract

New potential antifungal antibiotics UK-2A, B, C and D were elucidated as nine membered dilactone derivatives, isolated from mycelial cake of an actinomycete strain 517-02. In the continuation of a screening program in order to discover other useful bioactive metabolites from the same microbial sources, a novel UK-3 and two known compounds of UK-4 and UK-5 have been isolated.The molecular structure of UK-3 was very similar to UK-2A, except thai UK-3 did not have any methoxy group (-OCH3) on pyridine at ('-4'. UK-4 and UK-5 were identified as kown compounds of isocoumarine. These structures were elucidated based on their spectral and chemical evidence.Biological activity assay, demonstrated that UK-3 was active as against to Saccharomyces cerevisiae, Aspergillus niger dan Neurosporo sitophila and inhibited growth of cancer cell, P-388, B-16, KB and COLO-201.
The Synthesis of Quinidine Salicylate Ester Compound Intan Nurjaya; Muhammad Hanafi; Puspa D.N Lotulung; Teni Ernawati; Sri Mursiti
Jurnal Kimia Terapan Indonesia Vol 20, No 2 (2018)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.016 KB) | DOI: 10.14203/jkti.v20i2.403

Abstract

Quinidine a compound isolated from quinine plants, one of the species of quinine plants is (Chincona ledgereriana) From PT SIL Lembang. The purpose of this study was obtain quinidine salicylate ester through esterification reaction. In this study, the synthesis of quinidine ester compound by esterification reaction was conducted. Esterification reaction was conducted by using DCC activator and DMAP catalyst with one carboxylic acid namely salicylate acid producing new compound namely quinidine salicylate, Subsequent Quinidine salicylate was obtained in the form of oil with 97% yield. The compound obtained from the synthesis was then identified using Thin Layer Chromatography continue analyzed using with Spectrophotometer, LC-ESI-MS spectroscopy. Results show that the target compound has been successfully synthesized.
ISOLASI DAN PENENTUAN STRUKTUR MOLEKUL DARI TANA MAN OBAT TRADISIONAL SMILAX CORDIFORIA Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6559.429 KB) | DOI: 10.14203/jkti.v6i1-2.228

Abstract

A research was carried out to isolate the constituents of Smilax cordiforia trunk bark. It is a famous medicinal plant in Mexico, used as weight control. The isolation methods were carried out by maceration in ether, and remaceration in methanol, and then both of crude extracts were purified by using combined chromatography technique. In the purification and identitification of the ether extract two compounds as steroid saponins, 3-B-O -B-D-glucopyranosyl sitosterol (1) and a mixture of 6-O-palmitoyl-glucopiranosil sitosterot, 6-0stearylglucopyranosyl sitosterol and 6-0arachidoyl-glucopyranosyl sitosterol (2) were obtained, which also have been isolated from other medicinal plants. Isolation from methanol extract resulted in four known substance of phenolic derivatives, identified as afilbin (3) isoastilbin (4), engelitin (5). and chlorogenic acid (C). engelitin demonstrated antifungal activity, astilbin and chlorogenic acid showed antifungal, antibacterial, and antiviral activities. Chlorogenic acid also exhibited antimutagenic, antitumour and antioxcidant activities. Their molecular structures were detemined on the basis of combied spectroscopic methods including two dimensional NMR (2D NMR).
ISOLATION AND STRUCTURE ELUCIDATION OF A NEW OLEANOLIC ACID AND ITS SAPONINS FROM Thylacospermum caespitosum Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3392.134 KB) | DOI: 10.14203/jkti.v7i1-2.224

Abstract

A new oleanollc acid and its saponins have been isolated from methanol and ether extract of the whole plant of cushion plant T. caespltosum. The compounds were established as olean-12-en-3b-16a-diol-23,28-dioic acid (1), 3jJ-O-jJ-D-glucopyranosylolean12-en-16a-diol-23-doic acid (2), and 28-O-B-D-glucopyranosylolean--12-en3b, 16a-diol-23-dioc acid (3), which are trivial named as thylacospermic acid (1), thylacospermicoside A (2) and thylacospermicoside B (3), respectively. Three known steroids namely 22,23-dihydrospinasterol (4), a-spinasterol (5), and 3jJ-OjJ-D-glucopyranosylspinasterol(6) [lJ and the mixture of phytoceramides (7) [2, 8] were also isolated from the ether extract. The compounds were identified based on chemical and spectroscopic methods. This is the first report on the isolation of the compounds from genus Thylacospermum.
SKRINING IN SILICO POTENSI SENYAWA ALLICIN DARI ALLIUM SATIVUM SEBAGAI ANTIPLASMODIUM Fatmawaty Fatmawaty; Muhammad Hanafi; Rosmalena Rosmalena; Vivitri Dewi Prasasty
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.064 KB) | DOI: 10.14203/jkti.v17i2.33

Abstract

Allicin compound (2-propene-1- sulphinothioat acid S-2-propenyl ester) is known to have potential as antiplasmodium in vitro. However, the inhibitory activity mechanism of Allicin to plasmodium is still unknown. In this research, we determined the inhibitory activity of Allicin in silico. Identification of physicochemical properties of Allicin compound and two Allicin derivatives, Alc1, Alc2 and Ac2Alc3 were also conducted.. Furthermore, analysis of drug-likeness and adsorption-distribution-metabolism-excretion (ADME) were carried out on the Allicin compound and its derivatives to find the potential of these compounds as drug candidates. In determining the specific interaction, we utilized molecular docking analysis between Allicin and its derivatives against a protein target Cysteine Protease (SP). Molecular  docking results showed that Allicin derivative, Alc2 (S-prop-2-en-1-yl 3-methylbut-2-ene-1-sulfinothioate, C10H18OS2) has better potential as inhibitors than Allicin, based on the lower bond energies and the inhibition constants, thus Alc2 can be used as an antiplasmodium agent candidate.Keywords: Allicin, Allicin derivatives, drug likeness, ADME, molecular docking
Identification and Characterization of Bacterial Lipase from Plateu Soil in West Java Vivitri Dewi Prasasty; Vinella Winata; Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 18, No 02 (2016)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.891 KB) | DOI: 10.14203/jkti.v18i02.85

Abstract

Lipases are known as glycerol ester hydrolases that catalyze the hydrolysis of triglycerides into free fatty acids and glycerol. Lipases are found in human, animal, plant, and microorganisms. The aim of this research is to identify lipase producers and characterize bacterial lipase from West Java plateau soil. Plateau soil bacteria samples were isolated on lipase screening medium containing Rhodamine B. Olive oil was used as a substrate in screening and production medium bacterial lipases. From 16 bacterial isolate of lipase producers, 14 were identified as Bacillus sp. and the others were identified as Pseudomonas alcaligenes. All isolates were taken into production step to determine their lipase activities. Moreover, top 3 lipase activities out of 16 lipase activities were chosen to find the optimum pH and temperature. Both characterizations showed pH optimum and temperature optimum from each lipase. These optimum condition were used in heat stability characterization for each lipase samples. The result showed that lipase from isolate COK 2 in optimum pH 4 and temperature 50oC was the most stable lipase due to this sample has good and stable activity for 1 to 5 hours incubation time. Lipase sample from isolate COK 2 has good efficiency for lipase productivity in acid condition and high temperature. Results of this investigation could encourage utilization of these activity enhancers for various industrial applications.
ISOLATION AND STRUCTURE ELUCIDATION OF THE CUSHION PLANT POTENTILLA ARTICULATA Muhammad Hanafi; Shibata Kozo; Mamoru Kanzaki
Jurnal Kimia Terapan Indonesia Vol 6, No 1-2 (1996)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4543.362 KB) | DOI: 10.14203/jkti.v6i1-2.230

Abstract

Methanol extract of the whole plant of P. articulata Franch was fractionated by using open column silica gel chromatography following identification, resulted in the isolation of known phytoceramides, N-(2'-hydroxy-acyl)-2-amino1,3,4trihydroxy-8-octadecene) (1), which also isolated from Urtioca dioca and Thylacospermum caespitosum, and two known steroids, identified as ~-sitosterol (2), 3B-O-B-D-glucopyranosylsitosterol (3), which have also been isolated from Prunella vulgaris L. var. Iilacina (Labiatae). Triterpenoic acids were elucidated as 2B,19a-dihydroxyursolic acid (4), a mixture of Zcc-monohydroxyursclic acid (5) and 2a-monohydroxyoleanolic acid (6), also found in Geurn japonicum Thunberg, 19a-monohydro-xyursolic acid (7), a mixture ursolic acid (8) and oleanolic acid (9), which were also obtained in Isodon japonicus Hara. Their structure identifi-cations were based on chemical and spectroscopic methods.
PEMANFAATAN KULIT UDANG UNTUK PEMBUATAN KITOSAN DAN GLUKOSAMIN Muhammad Hanafi; Syahrul Aiman; D Efrina; B. Suwandl
Jurnal Kimia Terapan Indonesia Vol 10, No 1-2 (2000)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3278.112 KB) | DOI: 10.14203/jkti.v10i1-2.180

Abstract

Shrimp shells as side product of frozen shrimp industry isnot yet used in pharmaceutical or chemical industries. Shrimpshells has a chemical constituent called chitin, calcium carbonateand protein as main compounds. By decalcination in dilute aqueosHCl solution (1-2 N) and deproteination ill dilute aqueos NaOHsolution (3-4 Nj gives a chitin. Deacetylation of chitin in theexcess of aqueous 50 % NaOH solution produces chitosan. Inthe preleminary experiment indicated that water (< 10%) andash (< 2%) content of chit os an same as standard. Based on theexperiment result the optimum condition was obtained as 1N HCIsolution (1: 10), 3N NaOH solution (1:6) and 50 % NaOH solution(1: 5) resulted 12,61 % chitosan with deacetylation degree valueabout 70 % with FT1R methode. Hydrolysis of chitin or chitosanin excess HCl gives glucosamine about 17 %, it's a mixture of aand B-glucosa11line. Glucoseamine is identified using 1H and13C NMR spectrum.
ISOLATION AND IDENTIFICATION OF 9-METHOXYCANTHIN-6-0NE COMPOUND FROM Eurycoma longifolia ROOTS Sofa Fajriah; Muhammad Hanafi; Atiek Sumiati; Ngadiman Ngadiman
Jurnal Kimia Terapan Indonesia Vol 12, No 2 (2010)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2282.305 KB) | DOI: 10.14203/jkti.v12i2.213

Abstract

9-Methoxycanthin-6-one compound has been isolated from Pasak Bumi (E. Longifolia) roots. The isolation process using maseration, colum chromatography vacuum, and recrystallization techniques. E. Longifolia root maserated with n-hexane and methanol respectively. The methanol extract fractionated using column chromatography vacuum with gradient elution (hexane-etil acetate) to obtain 9 fractions. Fraction 5 further purification using recrystallization technique to obtain yellow crystal, identified by LC-MS and NMR spectroscopy as 9-Methoxycanthin-6-one.Keyword: E. Longifolia, isolation, spectroscopy, 9-Methoxycanthin-6-one.
Co-Authors . Hariyanti Abd Kahar Abdul Gani Abdul Mughni Ade Chandra Iwansyah Adipratiwi, Nadia Adnan, Andi Astina Afriza Media Ahmad Ramadani, Ahmad Ahmad Shohibul Wafa Akhmad Darmawan Alaiddin Koto, Alaiddin Ali Karmani Almasri Amalia Amalia Ameliah, Nurul Andi Karman, Andi Andi Sadapotto Andini Sundowo Andri Eko Putra Anggraini, Febi Ansumarwaty, Febiyanti Apriandini, Lisya Apriani, Ari Aprilia, Jesika Wanda Ardiansyah, Febry Aries Sukariawan Aris Doyan Arisanto, Ahmad Budi Ariyani, Titin Armanda Putra, Mochammad Ravi asma, Rasma Asmawati, Talitha Raissa Asrifan, Andi Aswadi Aswadi Aswadi Atiek Soemiati Atiek Sumiati Atmawinata, Maya R Aulia, Meilita Auliya, Isnie Azahra, Silena Azhari, M. Rozi Azim, Nur Santriani Utari Azizah, Arsyiah Salma B. Suwandl Bagaskara, Bagaskara Berna Elya Buhari Buhari Chatarina Umbul Wahyuni D Efrina Dadan Ridwanuloh Darwati Darwati Demastita, Ni Putu Fanty Desi Harneti Putri Huspa Desi Ramayanti Djamil, Ratna Dwi Astuti Ecca, Suleha Elidayani, Evi Ellyzena Wulandari Elviandri, Elviandri Endang Saepudin, Endang Erma Ermawan Ermawan ERWAHYUNI ENDANG PRABANDARI Erwin, Muh Evawani Elysa Lubis Fadlina Chany Saputri Farabi, Kindi Fatimah Azzahrah Fatimatushshalichah, Syayidati Fatmawati Fatmawati Fatmawaty Fatmawaty Fatonah, Astri Amaliah Fauzi Febrianto Fauzi, Kevin Azhar Fauziah Nur Ariza Fenny Bintarawati Firma Hafmi Fitri Rahayu Fitrianingtyas, Rizki Galuh Widiyarti Gatot Yudoko Ghapur, Muhammad Abdul Ghazali, Fikri Ghofardhan, Muhammad Ghozali, Arinaa Sabilah Gulo, Jelistin Gunawan Gunawan Hajijah, Humairah Hakiki, Dina Hany, Iga Permata Harsie, Sutag Hasan Hasan Hasan Hasan Hasanuddin Hasanuddin Hasibuan, Wildan Ansori Hasnaliah, Hasnaliah Hattu, Muhammad Ikhsan Hayu Dwimawanti, Ida Hendig Winarno Hendra Saputra Herry Cahyana Hidayat, Tegar Samudra Hizyam, Muhammad Huzaena, Mifta Idayanti Idayanti Idharul Haq Iis Irawatty Dewi Ika Mustika Ilhamuddin Ilhamuddin Ilhamuddin Ilhamuddin, Ilhamuddin Imam Fauji, Imam Intan Nurjaya Iranda Nadya Febianty Irmawadi, Zaibun Irwan Yon Hadi Islami, Tessa Jala, Jusrianto Jamaluddin Ahmad Jamilah Abbas Jemi, Renhart Jumatriadi Jumatriadi, Jumatriadi Jumiati Jumiati Jumiati Junedi, M Irfan Jusrianto K, Nuraini K, Rahmana Emran Kahar, Kurnia Putri Kamal, Kamal Kardono, Broto Sugeng Kasau, M Nurzin Kasman, Nuraini Kasmiati Khairunnisa, Shofiyah Khalik, Suhartini Khalik, Suhartini Kindy S, Mhd Al Kurnia Sofyan Kurniati Kurniati Kusmardi Kusmardi L, Syahrir Lababa Lababa Ladeska, Vera Lalu Aldi Lalu Muh. Fahri LALU RUDYAT TELLY SAVALAS Landong, Ahmad Lanta, Jumiati Lilik Kustiyah Linayanti Linda Maryani Lotulung, Puspa Dewi N. Lutpiyah Lutpiyah M Nurzin K M, Usman M. Idris M. Nurzin R. Kasau Mahise Okten Ramadhani Mahmud, Nurlaelah Mailawati Mamoru Kanzaki Manda, Ibrahim maria ulpah Marshalina, Afifah Marzuki Marzuki Masnawati Mau’ud, Mohamad Megawati, Meliantari, Dian Mhd.Furqan Mierza, Vriezka Minarti Minarti Moch. Chasani Mohammad, Amrija Mudzakir Nur Hidayatussani Muh. Zahirul Haq Usman Muh.Herianto Muhamad Rizal Martua Damanik Muhamad Zaril Gapari Muhammad Ihsan Muhammad Iksan, Muhammad Muhammad Nawir Muhammad Ridwan Muhammad Rizki Muhammad Zul Fahmi Muhammad, Firdaus Yusri Muhlis Mujib Ridwan Muliani Mulyana Mulyana Mulyani, Ridha Munir Anshory Muntari Muntari Murdani, Elisa Musdalifa Basri Nada Saniyah Nadirah, Nadirah Naf’an Tarihoran Nahdah, Khalilah Nurun Narrij Lotulung, Puspa Dewi Nasrullah Nasrullah Nasrullah Ngadiman Ngadiman Ngadiman Ngadiman Ni Luh Putu Pebri Artayani . Nia Aprilia Nina Artanti Nina Artanti Nindiyana, Baiq Nurmaulida NL, Puspa Dewi Nofrianto, Noke Norcahyono Norcahyono Nova Yohana Nur Asmah NUR FADILAH Nur Luthfi Hanif Al-Baihaqi Nur, Andi Herlina Nuraini K Nuraini Kasma Nurlaelah Mahmud Nurlelasari Nurlelasari Nurlia Arsyad Nurmala Nurmala Nurmala Nurmala Nurul Fadhilah Suardi Nurwahida Nurwahida, Nurwahida Nurwulan, Zulhilda Pandapotan Simbolon Pandiangan, Martina Pohan, Selamat Pramono, Sekardini Cahyaning Pramudito, Adrian Prastiwi, Mentari Dwi Puji Budi Setia Asih, Puji Budi Puspa D.N Lotulung Rahayu, Karmila Dwi Rahmat Mulyana Dali Rahmayani, Mentari Tri Indah Rahutomo, Suroso Raja Joko Musridho Ramadhan, Dany Ramdhani, Eka Putri Cahya Rani Maharani Rasyid, Rustam Efendy Razali, Mariany Renhart Jemi Rifa’i Rika Oktavia Damayanti Rina Hidayati Pratiwi Riska Handayani Rita K Sari Rita Kartika Sari Rohmat, Siti Saudah Rony, Zahara Tussoleha Rosa Dewi Pratiwi Rosmalena Rosmalena Rosmini Kasman Rusmaliati, Rusmaliati Rusny Edy Rustam Efendi Ruswati, Ruswati Ryandi Safitri, Noraya Safni Marwa Safrina, Suci Safutra, Ricky Indrawan Saifullah Saifullah Saifullah Sailal Arimi Sainab Sakiah Sakiah Salahuddin Salahuddin Saleh, Firman Salsabila Fitriyani Sam Hermansyah Samsul Hadi Sanati, Sanati Santy Rahmawati Saprizal Hadisaputra Sari, Riski Monno Sarifuddin Sarifuddin Sedijani , Prapti Senny Widyaningsih Septiasa, Alfia Muawanah Serli Sanggang Sarasta Shibata Kozo Silvia Fadila Siti Annisa Wahdiniawati, Siti Annisa Siti Isnijah Siti Sunariyati Siti Zulfa Sofa Fajriah Soleh Kosela Sri Karyati Sri Mursiti Suardi Zain, Suardi Subaidi, Mohammad Subhan Nooriansyah Sukranudin Suleha Suleha Ecca Suleha, Suleha Sulistiyowati, Arrin Suminar A Achmadi Suminar S Achmadi Suminar S Achmadi Sutag Harsie Syafrudin, Din Syahrir L Syahrul Aiman Syamsu T Syamsul Arifin Syamsul Bahri Syamsur Rizal Syufina, Arina Shofia T, Syamsu Tati Herlina Teni Ernawati Thowimma, One Tri Mayanti Tri Yuniningsih Ulpah, Kamaria Unang Supratman Undri Rastuti Uniawati Usman M Usniati, Ita Vinella Winata Vivitri Dewi Prasasty Wahyu Khalik Efendi wahyuni wahyuni Waluyo, Danang Wasrin Syafii Yanotama T Laksana Yanti Fitria Yasir ", Yasir Yayuk Andayani Yesi Desmiaty, Yesi Yoselina, Prima Yulianingrum, Aullia Vivi Yunni, Yunni Yusmah Yusmah Yusmah Yusmah, Yusmah Yusuf Amrozi Zozy Aneloi Noli Zulfahmi Zulfahmi