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All Journal Ulul Albab: Jurnal Studi dan Penelitian Hukum Islam Ahkam: Jurnal Ilmu Syariah Al-Manahij: Jurnal Kajian Hukum Islam Media Komunikasi Geografi El-HARAKAH : Jurnal Budaya Islam IJTIHAD Jurnal Wacana Hukum Islam dan Kemanusiaan Fibusi (Jurnal Online Fisika) Arena Hukum Nurani: Jurnal Kajian Syariah dan Masyarakat IJTIHAD Jurnal Kesehatan Gigi Al-Ihkam: Jurnal Hukum dan Pranata Sosial JPW (Jurnal Politik Walisongo) Sawwa: Jurnal Studi Gender Dentino: Jurnal Kedokteran Gigi JURISDICTIE Jurnal Hukum dan Syariah Jaqfi : Jurnal Aqidah dan Filsafat Islam JP3 (Jurnal Pendidikan dan Profesi Pendidik) KOMUNIKA AL QUDS : Jurnal Studi Alquran dan Hadis Al-Fikri: Jurnal Studi dan Penelitian Pendidikan Islam Journal of Islamic Education Policy Tadbir : Jurnal Studi Manajemen Pendidikan Jurnal Penelitian Pendidikan IPA (JPPIPA) JPK (Jurnal Pancasila dan Kewarganegaraan) Indonesian Journal of Islamic Educational Management Jurnal SOLMA Al-Hayat: Journal of Islamic Education Misykat al-Anwar Jurnal Kajian Islam dan Masyarakat NUR EL-ISLAM : Jurnal Pendidikan dan Sosial Keagamaan JMKSP (Jurnal Manajemen, Kepemimpinan, dan Supervisi Pendidikan) Bunda Edu-Midwifery Journal (BEMJ) JURNAL PENDIDIKAN TAMBUSAI Jurnal Basicedu Journal of Educational Research and Evaluation JURNAL AGRIBISAINS AL-TANZIM : JURNAL MANAJEMEN PENDIDIKAN ISLAM Gorontalo Development Review JASa (Jurnal Akuntansi, Audit dan Sistem Informasi Akuntansi) MUWAZAH: Jurnal Kajian Gender HIKMAH : JURNAL PENDIDIKAN AGAMA ISLAM Jurnal Abdimas Mahakam CERIA (Cerdas Energik Responsif Inovatif Adaptif) PrimaryEdu - Journal of Primary Education Jurnal Bidang Ilmu Kesehatan Forum Tarbiyah Jurnal Mantik Borneo Nursing Journal (BNJ) JAIM (Jurnal Abdi Masyarakat) Jurnal GeoEkonomi Ulumuna: Jurnal Studi Keislaman Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Best Journal (Biology Education, Sains and Technology) Islamic Insights Journal Dakwatuna: Jurnal Dakwah dan Komunikasi Islam Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Sakina: Journal of Family Studies NEGARA DAN KEADILAN Sriwijaya Journal of Ophthalmology IQ (Ilmu Al-qur'an): Jurnal Pendidikan Islam Emanasi Jurnal Ilmu Keislaman dan Sosial As Sibyan : Jurnal Kajian Kritis Pendidikan Islam dan Manajemen Pendidikan Dasar Rausyan Fikr : Jurnal Pemikiran dan Pencerahan INVEST : Jurnal Inovasi Bisnis dan Akuntansi Texere Jurnal Res Justitia : Jurnal Ilmu Hukum Indonesian Journal of Counseling and Development Journal of Midwifery and Public Health Education Achievement: Journal of Science and Research Jurnal Abdimas Kartika Wijayakusuma JURNAL PAI: Jurnal Kajian Pendidikan Agama Islam JIPMukjt:Jurnal Ilmu Pendidikan Muhammadiyah Kramat Djati Jurnal Sistem Informasi Bisnis (JUNSIBI) Edu Sociata : Jurnal Pendidikan Sosiologi Indonesian Journal of Business Analytics (IJBA) Kolaborasi Jurnal Pengabdian Masyarakat Journal of Early Childhood and Character Education Jurnal Ekonomika: Manajemen, Akuntansi & Perbankan Syari'ah Jurnal PkM MIFTEK Obor: Oikonomia Borneo Jurnal Alasma : Media Informasi dan Komunikasi Ilmiah Indonesia Law Review (ILREV) Legal Protection for the Partnership Agreement Parties International Journal of Education and Teaching Zone Jurnal Basicedu An-Nashiha: Journal of Broadcasting and Islamic Comunication Studies Research and Development in Education (RaDEn) Jurnal Salingka Abdimas Journal of Education Technology Information Social Sciences and Health Peradaban Journal of Religion and Society Peradaban Journal of Law and Society eProceedings of Engineering Gunung Djati Conference Series Jurnal Inovasi Pengabdian dan Pemberdayaan Masyarakat Jurnal Pengabdian kepada Masyarakat Innovative: Journal Of Social Science Research Madani: Multidisciplinary Scientific Journal Jurnal Sahid Mengabdi Jurnal Psikologi dan Konseling West Science PharmaCine : Journal of Pharmacy, Medical and Health Science JOURNAL EDUCATIONAL MANAGEMENT REVIEWS AND RESEARCH Journal of Education Method and Learning Strategy Interdisciplinary International Journal of Conservation and Culture Jurnal REP (Riset Ekonomi Pembangunan) Jurnal Pendidikan Islam Anak Usia Dini (Anaking) Sanaamul Quran : Jurnal Wawasan Keislaman Cendikia Pendidikan AN-NAS: Jurnal Pengabdian Masyarakat Wisesa: Jurnal Pengabdian Masyarakat Al Itimad: Jurnal Dakwah dan Pengembangan Masyarakat Islam Menara Tebuireng : Jurnal Ilmu-Ilmu Keislaman West Science Islamic Studies Journal of Regional Public Administration Insya Al-Muhith : Jurnal Ilmu Qur'an dan Hadits JGEN : Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmu Al-Qur'an dan Tafsir Attadrib: Jurnal Pendidikan Guru Madrasah Ibtidaiyah JER (Jurnal Ekonomi Regional) El-Waroqoh : Jurnal Ushuluddin Dan Filsafat Jurnal Pengabdian Kepada Masyarakat Kesehatan Galuh Jurnal Bidan Cerdas Jurnal Sains dan Kesehatan Mandalika Journal of Medical and Health Studies Jurnal Abdimas Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pengabdian Masyarakat Kesehatan Terkini Digital Theory, Culture & Society MANAGIERE: Journal of Islamic Educational Management Nusantara Innovation Journal Munazzama Journal of Islamic Management and Pilgrimage Al-I'timad: Jurnal Dakwah dan Pengembangan Masyarakat Islam Jurnal Bioteknologi & Biosains Indonesia
Siti Rohmah
Universitas Muhammadiyah Jakarta
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Sosialisasi Peningkatan Pengetahuan Pus Terhadap Penggunaan Kontrasepsi IUD Di Wilayah Kerja Puskesmas Gardujaya Rohmah, Siti; Fatimah, Siti; Heryani, Sri
JPKU: Jurnal Pengabdian Kesehatan Unigal Vol 1, No 1 (2024): April
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/jpkmu.v1i1.14017

Abstract

Di Indonesia penggunaan kontrasepsi IUD dan lain-lain 1,11%, Peserta Aktif (PA) atau angka prevalensi kontrasepsi (CPR) di Jawa Barat mencapai 73,74%. Kabupaten Ciamis didapatkan hasil Puskesmas Gardujaya diperingkat ke dua terakhir yang memiliki capaian kontrasepsi IUD terendah dengan capai kumulatif hanya 5 orang akseptor (0,3%) sedangkan data tahun 2020 adalah sasaran jumlah 1128 orang dengan penggunaan KB IUD sebanyak 11 orang (0,97%) penurunan penggunaan IUD sebesar (0,4%).Tujuan penelitian ini adalah untuk mendeskripsikan faktor predisposisi rendahnya penggunaan alat kontrasepsi Intra Uterine Device (IUD) di Wilayah Kerja Puskesmas Gardujaya Tahun 2022. Metode penelitian ini menggunakan jenis penelitian deskriptif Sempel dalam penelitian ini sebanyak 100 orang, Teknik pengambilan sampel dilakukan secara acak terhadap wanita usia subur yang memenuhi kriteria inklusi dan eksklusi. Penelitian ini mendapatkan hasil sebagian besar responden memiliki pengetahuan cukup sebanyak 50 orang (50%). Sebagian besar dari responden tidak mendapatkan dukungan suami sebanyak 58 orang (58%). Sebagian besar dari responden memiliki pendapatan tidak sesuai UMR sebanyak 62 orang (62%). Kesimpulan: Sebagian besar responden memiliki pengetahuan cukup dan Sebagian besar dari responden tidak mendapatkan dukungan suami, Sebagian besar dari responden memiliki pendapatan kurang. Saran: Diharapkan masyarakat dapat meningkatkan pengetahuan atau mengikuti penyuluhan tentang tentang KB untuk mengsukseskan program pemerintah melalui program KB.
Analisis Peningkatan Kemandirian Belajar Melalui Model Pembelajaran Visual Auditory Kinesthetic (VAK) dengan Teknik Whole Brain Teaching (WBT) Rohmah, Siti; Widhyahrini, Khusna; Maslikhah, Maslikhah
Attadrib: Jurnal Pendidikan Guru Madrasah Ibtidaiyah Vol. 6 No. 1 (2023): Pendidikan Dasar Anak
Publisher : Institut Agama Islam Daruttaqwa Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54069/attadrib.v6i1.376

Abstract

Tujuan penelitian yaitu mengetahui perencanaan penggunaan model VAK dengan Teknik WBT; melihat bagaimana keterlaksanaan penggunaan model pembelajaran; serta bagaimana evaluasi kemandirian belajar melalui model pembelajaran VAK dengan teknik WBT dapat meningkat. Metode yang digunanakan yaitu desain deskriptif kualitatif melalui studi kasus. Data yang diteliti didapat melalui wawancara, observasi, tes/angket, dan dokumentasi. Penelitian menunjukkan hasil yaitu: Pertama, rencana dalam rancangan kegiatan belajar-mengajar berjalan dengan tahap-tahap yang sistematis sesaui diharapkan melalui model VAK dengan teknik WBT, tahapan berupa perencanaan, pelaksanaan, dan evaluasi. Kedua, Pelaksanaan dan penerapan dalam penyampaian materi ditunjukkan melalui video yang kemudian diliat, dibaca dan dipahami oleh siswa melalui power point, sehingga proses pembelajaran dapat berjalan dengan lancar. Ketiga, Evaluasi dan peningkatan kemandirian dalam belajar dapat dilakukan dengan diskusi kelompok, pemberian tugas, praktik secara langsung, dan pemberian soal latihan. Hasil evaluasi pada kelas VA menunjukkan bahwa hasil belajar siswa memeroleh peningkatan yaitu dari yang semula 60% menjadi 85% setelah menggunakan model pembelajaran VAK dengan teknik WBT, hal ini dibuktikan dengan adanya peningkatan hasil evaluasi belajar yang sangat baik karena terlaksanaya kemandirian dalam belajar. Sedangkan hasil evaluasi selama proses pembelajaran pada kelas VB setelah menggunakan model VAK dengan teknik WBT terjadi penurunan dari yang semula 50% menjadi 45%, hal ini menjadi bukti bahwa adanya penggunaan model VAK dengan teknik WBT pada pembelajaran IPA tidak dapat meningkatkan kemandirian belajar siswa. Hasil perolehan belajar juga dilihat pada peningkatan rata-rata nilai hasil pre test 70,26 dengan persentase 60% dan 68,78 dengan persentase 50% siswa tuntas belajar dan hasil rata-rata nilai post test yaitu 93,25 persentase 85% dan 77,4 persentase 45% siswa selesai belajar.  
The Influence of Professionalism, Locus of Control, Emotional Intelligence and Professional Ethics on BPR Audit Quality with Ambiguity as a Moderator Dina Safitri; Rohmah, Siti; Salta, Salta
Indonesian Journal of Business Analytics Vol. 4 No. 2 (2024): April 2024
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/ijba.v4i2.8730

Abstract

As an independent party, auditors have the opportunity to improve the quality and reliability of company financial reports that are used by stakeholders in making decisions. Against this background, incidents relating to the accounting profession from an audit quality perspective are increasing. Incidents often occur with test objects due to the inspector's incompetence. The loss of public trust in the accounting profession is an indication of the loss of AI's strict internal audit concept and a weak governance system. This research aims to determine how professionalism, locus of control, emotional intelligence, and professional ethics influence audit quality. auditors who participated in this research working in BPR Region III in Cirebon, West Java, were used as respondents. The resulting sample consisted of 49 of the 83 research topics selected to represent BPR researchers. The results show problems with normality, multicollinearity, and autocorrelation in the classical assumption test, which consists of normality, multicollinearity, and heteroscedasticity tests using a one-stage multivariate model. The remainder is influenced by other invisible variables, with a total influence of 0.598 or 59.8%.  
Aplikasi Kitosan dari Litopenaeus vannamei dan Limbah Baglog dari Pleurotus ostreatus untuk Mendekolorisasi Limbah Cair Batik Oktavia, Swastika; Rohmah, Siti; Novi, Cory
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.5859

Abstract

The batik sector generates wastewater containing pigment residue, posing a significant environmental threat if not managed effectively. L. vannamei shells yield chitin, convertible to chitosan for water treatment. Baglogs, comprising mycelium and cellulose, serve as a viable adsorbent. The purpose of this study was to learn about the use shell of L. vannamei and P. ostreatus baglog waste with different lengths of time to decolorization batik industry waste water. The method for testing involves deproteinization with NaOH 1M, demineralization with HCl 2M and deasetylation with NaOH 60%. Chitosan L. vannamei and baglog P. ostreatus (2:25 g/g) waste were put into a container containing 500 mL of batik wastewater with colour content with variations of 0, 12, 24, 48, 72, 96, and 120 hours. Further, the capacity of adsorption chitosan and baglog waste are determined using a UV-Vis spectrophotometer during the decolorization process in batik industry waste water using shell of L. vannamei and P. ostreatus baglog waste are 96 hours with a decolorization rate of 90.05%.
The Effectiveness of Chain Pumpkin on Improving Breast Milk Production and Increasing Baby's Weight Umairo, Gusrida; Kartini, Titin; Hastuti, Yessi; Rohmah, Siti; Jamilah, Siti Nurlaela
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8877

Abstract

A Breast milk is the best nutrition for babies because it is easy to digest and contains the nutrients needed for growth and immunity. This research aims to determine the effectiveness of chayote in smoothing breast milk production and increasing baby weight. This research uses a two group pretest-posttest design method without a control group, with measurements taken before and after treatment. The independent variable is the method of consuming chayote, while the dependent variable is the smooth production of breast milk and the increase in baby weight. This research produced information that the P-Value value obtained for increasing the smoothness of breast milk production (0.000 for boiled chayote and 0.001 for fried chayote) showed a significant difference. The P-Value value obtained for increasing baby weight (0.000 for boiled chayote and 0.003 for fried chayote) shows a significant difference. Based on these data findings, breastfeeding mothers are expected to consume chayote, both boiled and fried, as part of their diet to support smooth breast milk production and baby growth.
Theological Meaning of The Munggahan Ramadhan Ritual in Bandung, West Java Komarudin, Didin; Rohmah, Siti
el Harakah: Jurnal Budaya Islam Vol 24, No 1 (2022): EL HARAKAH
Publisher : UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/eh.v24i1.16373

Abstract

Rituals are sacred religious teachings based on beliefs that will give birth to peace and blessings in the life of the believers. One of them is the Munggahan ritual which is still carried out by the people of Bandung, West Java. This study aimed to determine how the ritual process was carried out and to understand the theological meaning of the Munggahan ritual carried out by the people of Bandung. The design used in this research is descriptive method that systematically explains the facts in the field through the anthropological approach of religion. The data collection process employed observation and interview. The informants were the chairman and several members of the Bandung community. The result shows that the theological meaning of the Munggahan ritual in the month of Ramadan is a form of cleansing oneself from bad things during the past year as well as a form of expression of gratitude for what Allah has given. It also conveys the hope to be given the convenience, safety, health and to avoid bad deeds during worship during Ramadan. Ritual merupakan ajaran agama yang sakral berdasarkan kepercayaan yang akan melahirkan kedamaian dan keberkahan dalam hidup penganutnya. Salah satunya adalah ritual Munggahan yang masih dilakukan oleh masyarakat Bandung, Jawa Barat. Tujuan dari penelitian ini adalah untuk mengetahui bagaimana proses ritual itu dilakukan dan untuk memahami makna teologis dari ritual Munggahan yang dilakukan oleh masyarakat Bandung. Metode yang digunakan dalam penelitian ini adalah metode deskriptif yang menjelaskan secara sistematis fakta-fakta di lapangan melalui pendekatan antropologi agama. Proses pengumpulan data menggunakan observasi dan wawancara. Informan adalah ketua dan beberapa anggota masyarakat Bandung. Hasil penelitian menunjukkan bahwa makna teologis dari ritual Munggahan di bulan Ramadhan adalah sebagai bentuk pembersihan diri dari hal-hal buruk selama satu tahun terakhir sekaligus sebagai bentuk ungkapan rasa syukur atas apa yang telah diberikan oleh Allah SWT. Juga menyampaikan harapan agar diberikan kemudahan, keselamatan, kesehatan dan terhindar dari perbuatan buruk selama beribadah di bulan Ramadhan.
Implementation of Religious Teacher Leadership in Cultivating Islamic Ethics in Madrasah Solong, Najamuddin Petta; Mokodenseho, Sabil; Rohmah, Siti
JURNAL AL-TANZIM Vol 8, No 2 (2024)
Publisher : Nurul Jadid University, Probolinggo, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33650/al-tanzim.v8i2.6730

Abstract

This research analyzes the implementation of religious teacher leadership in cultivating Islamic ethics at Madrasah Aliyah Negeri 1 Gorontalo City. This study employs a qualitative descriptive approach with a phenomenological framework. Data collection involves observation, interviews, and documentation. Data analysis follows four stages: condensation, coding, categorization, and theorization. The findings reveal that religious teachers can design, organize, guide, and supervise the inculcation of religious teachings, particularly in Islamic jurisprudence, thus emphasizing the manifestation of Islamic ethics, such as dress code, religious traditions, and Islamic interactions. However, the teachers' skills in critical thinking, collaboration, communication, and creativity have yet to integrate the practice of religion within them, both among teachers and students. This research underscores the importance of further studies to unveil comprehensive strategies for realizing Islamic ethics across various dimensions in the context of religious teacher leadership. These strategies should align with the student profile project of "rahmatan lil alamin" and position the practice of religious teachings as a critical leadership competency within the madrasah context.
Perancangan Strategi Komunikasi Pemasaran Dengan Menggunakan Metode Qspm (Studi Kasus: Usaha Mikro Kecil Menengah (Umkm) Ar-Riaz) Rohmah, Siti; Dellarosawati , Maria; Yastica, Tiara Verita
eProceedings of Engineering Vol. 11 No. 3 (2024): Juni 2024
Publisher : eProceedings of Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Usaha Mikro Kecil dan Menengah memiliki peran yang signifikan dalam pembangunan ekonominasional. Saat ini, UMKM diharapkankan dapat meningkatkandaya saing melalui inovasi. UMKM Ar-Riaz merupakan usahayang bergerak di bidang konveksi yang menjual baju kokomuslim laki-laki, jubah gamis dan mukena. Jangkauan pasaryang dapat dicapai dengan WOM sangat terbatas. Tujuan daripenelitian ini adalah UMKM Ar-Riaz ini sangat tergantungpada pembeli yang akan kembali membeli produk mereka danpromosi yang dilakukan oleh pelanggan dengan menggunakanstrategi komunikasi pemasaran dengan menggunakan metodeQSPM. Dalam perancangan strategi ini dilakukan dikarenakantarget penjualan tidak terpenuhi untuk setiap bulannya,terdapat produk musiman yang akan dibeli untuk hari raya idulfitri. Terdapat beberapa langkah yang melibatkan beberapamatriks analisis seperti factor internal dilakukan denganmenganalisis Marketing Mix (5P) dan melakukan wawancarakepada owner serta karyawan. Dan untuk factor eksternaldilakukan dengan mengguanakan analisis PEST untukmembantu UMKM Ar-Riaz menemukan strategi yang efektifdan efisien untuk melakukan komunikasi pemasaran.Bedasarkan penelitian yang telah dilakukan didapatkanperhitungan nilai Total Attractiveness Score (TAS) denganstrategi alternatif yang telah disetujui yaitu melakukanpenjualan dengan menggunakan online selling dengan livestreaming pada media e-commerce seperti Shopee atau TikTodengan niali TAS tertinggi sebesar (6,8037), Menambahkanstok ketersediaan produk di Gudang (6,3829), Penambahanwarna pada setiap produk untuk menarik pangsa pasar(6,0693). Kata kunci — Strategi Pemasaran, UMKM, Analisis SWOT, QSPM
Kendala-kendala Anak Usia Dini dalam Mengamalkan Al-Qur’an di Era Digital Rohmah, Siti; Sutrisno, Sutrisno
Journal of Early Childhood and Character Education Vol. 4 No. 1 (2024)
Publisher : FITK UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/joecce.v4i1.19451

Abstract

All Muslims believe that the Koran is the main source of Islamic teachings. The above are mandatory religious teachings, and a person cannot be called a good Muslim if he violates these beliefs. Thus, every Islamic educational institution must truly make the Koran the main material that must be studied. Read or recite the Koran, write, memorize, and be able to practice the religious values contained in the Al-Qur'an. Using the Koran as a guide to life is a competency that Muslim children must have. So what are the obstacles for young children in practicing the Koran? The above is a big problem in this research, the answer to which must be sought through a qualitative approach. The research subjects were 3 teachers and students at Nw Sajang Kindergarten. In order to obtain accurate data, researchers used interview and observation techniques. Based on the results of research and discussions that have been carried out, there are two obstacle factors, namely internal and external factors experienced by Nw Sajang Kindergarten children in learning to practice the Al-Qur'an. Also internal factors are: (1). Lack of concentration. (2). Difficulty reading Arabic letters. (3). Limited understanding of Arabic. (4). Pressure or lack of time for studying religion, especially the Koran. (5). Lack of motivation and strong will from the child. And the external factors are: (1). Environmental disturbances (2). Children play more often. (3). Lack of appropriate learning materials. (4). Children use electronic devices more often when they are at home. And the solution to overcome this obstacle is; increasing concentration, taking an educational game approach, integrated Arabic language teaching, effective time management, providing ongoing motivation, and finally creating a calm learning environment.
DEVELOPMENT OF PLASMID-BASED FOR EXTERNAL CONTROL MATERIALS OF CYP2D6*10 (rs1065852) GENE PCR-BASED DETECTION Malau, Jekmal; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Rahmasari, Ratika; Raekiansyah, Muhareva; Rohmah, Siti; Meilani, Nanda Diva; Septi, Annisa Frastica; Zahro, Aurora Fatimatuz; Annajla, Fathina; Hermosaningtyas, Anastasia Aliesa; Hilmi, Indah Laily
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2557

Abstract

Reliable clinical diagnosis of Single Nucleotide Polymorphisms (SNPs) is necessary for personalizing tamoxifen medication according to CYP2D6*10 genetic variations. Our research aimed to create a recombinant plasmid for external control material with a molecular size of 3812 bp. The recombinant plasmid was achieved by cloning an 838 bp gene insert of CYP2D6*10 rs1065852 into a 2974 bp pJET1.2 plasmid into Escherichia coli DH10B and selection on ampicillin agar medium. Isolated E. coli recombinants provided the plasmid molecules for analysis. Bi-directional sequencing and Real-Time PCR confirmed the presence of wild-type and mutant rs1065852 DNA fragments in the plasmid, namely homozygote CC and TT. The conclusion is that we have successfully introduced a novel recombinant plasmid developed by cloning the SNP rs1065852, which carries the 100C>T mutation, using pJET 1.2/blunt system, which could significantly enhance the accuracy of clinical SNP diagnostics for personalized medicine in breast cancer treatment.
Co-Authors A Halim A'la, Hadika A'zul A. Suhandi AA Sudharmawan, AA Abdul Azis Abdul Salam Noh Se Ik Abdul Wahid Abdurrahman, Haris Abrori, Abrori Adi Soeprapto Aditama, Risma Aditya Rahman afriansyah, Sahrul Afriyanti Afriyanti Afriyanti, Afriyanti Agista, Devi Ega AGUS RIYANTO Agus Riyanto Agus Suprapto Ahmad Barizi Ahsanal Kasasiah Aini, Nur Fadilah Ainol Ainol, Ainol Akmal Akmal Al-Azwi, Nadhifah Mizana Al-Husein, Fajar Alam, Syah Khalif Alexander, Dievon Firdaus Ali Muhdi, Ali Alifa Nur Fitri aliyya, Lisana shidqin Alvan Firdaus Amalia, Firda Dini ANANTA, ASTI Andi Rosa Anggraeni, Andini Anceu Anggraeni, Dinnie Anjani, Ria Annajla, Fathina Ansori, Ibnu Hajar Anugrah, Dena Sri Apriani, Zenita Arib, Juhrah M. Arif, Zaenal Ariyanto, Elvira Julia Ary, Maxsy As-Suvi, Ahmad Qiram Ashifa, Riswati Auliah, Nur Patul Aviva Febianti, Yola Ayu, Aulia Suminar Azhari, Padli Aziz, Adit Mohammad Bakhiet, Bakri Muhammad Bharata, Risma Wira Bilung, Noviana Busahdiar, Busahdiar Cahyadi, Yoga Ari Cahyani, Winda Dwi Cahyono, Hadi Catarina Wahyu Dyah Purbaningrum, Catarina Wahyu Dyah Cecep Hilman Chairuddin, Sugeng Cory Novi Dachlan, Rudy Syafariansyah Damayanti, Mutia Siti Dede Margo Irianto, Dede Margo Dede Trie Kurniawan Dellarosawati , Maria Desi Efrianti Dety Mulyanti Diah Mutiara Diani Aliansy Diansyah Permana Dina Safitri Dini Nurbaeti Zen Dwi Saputri, Rena Nugraini Edi Kurniawan Farid Efendi, Didik Ekasari, Rosdiana Elriska, Ripelian Elsa Agustina Elvania, Nindy Callista Endang Saeful Anwar Erfaniah Zuhriah Erlinda, Elsa Erna Budiarti Evllyshin Pakadang Fachry Abda El Rahman Fadil SJ Fahrurazi Fahrurazi Fakhrurazi Fakhrurazi Farhani, Siti Aulia Faris Rahmat Hidayat Fatah, Muhamad Abdu Fathimah Nadia Qurrota A'yun Fattah, M. Yusuf Faujiah, Syifa Ramdani Fenny Roshayanti Firawati, Andi Fani Firdaus, Firna Nakhwa Firmansyah Firmansyah Fitriyani, Fera Maya Franola, Frida Azmil Galib, Andi Muhammad Ganindha, Ranitya Gian Nova Sudrajat Nur Hadi Sasana Hadiyanto hana lestari Hanum, Nissa Hapsari , Nadya Ayu Hardaniyati, Hardaniyati Hari Susanti Haryani, Iis Hasanah, Tita Hasbullah, M. Farrij Hastuti, Yessi Hayati, Nur Rohmah Hendra, Tomi Herawati Herawati Heriyanti, Silvia Widyani Hermosaningtyas, Anastasia Aliesa Heryani, Sri Hikmatul Luthfi Himmatul Miftah Huda, Ali Anhar Syi’bul Humairah, Rika Husain Husain Hutauruk, Martinus Robert I, Ikrima Ibrahim, Galih Ichrom Nahzi, Muhammad Yanuar Ilahi, Restu Prana Imamudin Imamudin Imsak Rochmadi Indah Laily Hilmi IRMA YULIANTINA, IRMA Irsyad Irwansyah, Silvana Lestari Isnanto Isnanto Izzah, Ismatul Jamami, Yadziz Siddik Al Jamilah, Siti Nurlaela Jayawarsa, A.A. Ketut Jekmal Malau Joko Waluyo Khasnah Saidah Kholima, Fita Kholish, Moh. Anas Kristiawan, Diky Kurniawan, Dede Irwan Kuswinarti Kuswinarti Lestari, Rosalia Widhiastuti Luth, Thohir M. Astri Yulidar Abbas M. Hapis Ray Magdalena, Caroline Julyana Mala, Ilhami Shofiatul Mamonto, Merdi F. Mardiyyah, Sayyidatul Marzuki Mustamar Maslikhah Maslikhah, Maslikhah Maswiyah Maya Fitria Mayasari, Nanny Meilani, Nanda Diva Meishanda Putri Komara Mukti, Nikita Mokodenseho, Sabil Mowilos, Stevani Muh Idris, Muh Muhammad Alif Muhammad, Faiz Mahatir Muharam, Muhammad Faisal Muhareva Raekiansyah Mukti, Arifah Septiane Mulyani, Asifa Dewi Muthmainnah, Anti N. Oneng Nurul Bariyah Nabillah, Shafiyyah Salma Nadia Safira Nadiya Yunan Nafisa, Siti Najamuddin Petta Solong Nandang Nandang Negara, Julius Galih Prima Nengsih, Luluk Wahyu Nihan , Yumareta Anggun Ningrum, Mirza Cahya Ningrum, Widya Maya Ningsih, Rika Widia Ratna Nisita, Diyah Eni Noor Azura Zakaria Novebriansyah, Rizky Noviani, Dina Nur Chanifah Nur Fitri, Alifa Nur Lina Afifah Litti Nur Patul Auliah Nurdiana Tri Mulatsih Nurhuda, Abid Nurjanah Nurlatifah, Sarah NURUL HIDAYAH Oldiantho, Okky P. Pardede, Pantas Pamungkas Emnoor, Arief Ajie Pamungkas, R Wisnu Prio Pantas P Pardede Pardede, Pantas Pangondian Pitriani, Eliya Pranoto, Ahmad Hadi Praptawati, Destary Prihantini Prihantini Purbarningrum, Catarina Dyah Purnaman, Boby Yasman Purnamasari, Kurniati Devi Putri, Icha Aulia Qorinah Estiningtyas Sakilah Adnani Raharja, Reza Mauldy Rahayu, Lia Novi Nur Rahma, Dinda Rahmadini, Novia Eka Rahman Rahman Rahmasari, Ratika Rahmat Satoto Rahmatika, Aline Amanda Rajiv Nazry Faizullah Sina Gula Rakhman, Patra Aghtiar Ramadhan, M. Zilhan Salman Ramadhanty, Anisya Ramdhani, Tio Ratna Sari Dewi Rd. Heri Solehudin Retia, Iga Retna Yunita, Arifia Rhohmah, Afuin Neily Rifda Rini Fatma Kartika RIRIN LESTARI Riyani, Amelia Rizky, Muhammad Naufal Robert, Martinus Rohmah, Islamiatur Rohman, Asep Abdul roky_yonatan RR. Ella Evrita Hestiandari Rumi Suwardiyati Ruswati, Ruswati Saleh Hidayat Salis Irvan Fuadi Salta, Salta Samsudin, Abu Santoso, Blegoh Iwan Saraya, Sitta Sari, Shinta Puspita Sarib, Suprijati Sastradipraja, R Muhammad Arya Fajar Saumi, Ira Nurachma Sa’diyah, Sa’diyah Septi, Annisa Frastica Septiana, Eka Salsa Putri Septiono Eko Bawono setiawati, erni Siang, Diana Sintia, Sintia Sita, Nur Siti Aliyah Siti Fatimah Siti Hamidah Soetrisna, Denny Sri Hidayati Subhan Widiansyah Sudrajat Sudrajat Sugiarto S Suhara, Ade Suharsiwi Suharsiwi Suindartini Sukamto, Anton SULASTRI Sulwan Permana Sumiati, Eet Suminar, Ratna Suryadinata, M. Suryanli, Aurellia Leonny Sutarti Sutarti Sutrisno, Sutrisno Swastika Oktavia Syaidah, Khasnah Syaikhoni Jazuli Syaikhoni, Syaikhoni Syarifah, Maulida Syifa, Mar’atus Thomas Tiara Verita Yastica Tiara, Indah Leandra Tika Taruk Padang Titin Kartini Tohari, Ilham Tri Putri, Deby Kania Tria Desinta, Silvia Ayu Trimulyo, Joko Tuah Nur Tutik Hamidah Ulfah, Yeniati Umairo, Gusrida Umkabu, Talabudin Vip Paramarta Wahyudi Wahyudi Wahyuti, Sri Widhyahrini, Khusna Windarsih, Chandra Asri Yulianti Yulianti Yunan, Nadiya Yusdiarti, Arti Yusroh Zaenal Muttaqin Zahra, Aliya Azkia Zahro, Aurora Fatimatuz Zaini Muchlis Zakiyatul Badriyah Zohuri, Bahman