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Efek Pemberian Ekstrak Etanol Akar Rumput Belulang (Eleusine indica l. Gaertn) Terhadap Penurunan Jumlah Bakteri pada Mencit (Mus musculus) yang Diinokulasi Salmonella typhi Idhul Ade Rikit Fitra; Sahidin Sahidin; Abdul Karim
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 1, No 1 (2013)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.5 KB) | DOI: 10.46496/medula.v1i1.185

Abstract

Penelitian ini bertujuan untuk membuktikan efek dari pemberian ekstrak etanol akar rumput belulang (E. indica L. Gaertn) terhadap penurunan jumlah bakteri pada hepar mencit (Mus musculus) yang diinokulasi S. typhi.Penelitian ini adalah penelitian eksperimental laboratorium dengan rancangan the post-test only control group design. Dua puluh ekor mencit dibagi menjadi 4 kelompok, satu kelompok kontrol dan tiga kelompok uji. Kelompok I tidak diberi ekstrak etanol akar rumput belulang sebagai K. Kelompok II diberi ekstrak etanol akar rumput belulang dengan dosis 10 mg/ekor sebagai Uji I. Kelompok III diberi ekstrak etanol akar rumput belulang dengan dosis 20 mg/ekor sebagai Uji II. Kelompok IV diberi ekstrak etanol akar rumput belulang dengan dosis 40 mg/ekor sebagai Uji III. Semua kelompok diberi pakan standar pada hari ke-1 sampai hari ke-7. Pada hari ke-8 mencit diinokulasi S. typhi 1 x 10 6, setelah 2 jam mencit diberi ekstrak etanol akar rumput belulang pada kelompok uji dan Aquadest pada kelompok kontrol. Setelah 24 jam mencit didekapitasi dan diambil heparnya untuk menghitung jumlah koloni bakteri pada jaringan hepar. Rerata jumlah bakteri pada pengenceran ke-2 didapatkan kelompok K = 26.78, kelompok Uji I = 28.28, kelompok Uji II = 24.74, dan kelompok Uji III = 7.98. Uji Mann-Whitney U didapatkan perbedaan signifikan antara kelompok K dengan Uji III p=0.008, Uji I dengan Uji III p=0.007, dan Uji II dengan Uji III p=0.009(p=<0,05).Ekstrak etanol akar rumput belulang dapat menurunkan jumlah koloni bakteri pada mencit yang diinokulasi S. typhi pada tiap-tiap dosis.Kata Kunci : Akar rumput belulang, Koloni bakteri, Hepar, Salmonella typhi
Antioxidant and Cytotoxic Properties of Soft Coral Nepthea sp. Baru Sadarun; Nur Syifa Rahmatika; Agung Wibawa Mahatva Yodha; Adryan Fristiohady; Andini Sundowo; Syarul Nataqain Baharum; Idin Sahidin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 27, No 1 (2022): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.27.1.29-36

Abstract

Soft coral Nepthea sp. grows in the seas of South-East Sulawesi, Indonesia. However, information on the chemical and pharmaceutical aspects of this genus is still limited. Therefore, this research aims to explore the chemical contents and biological activities of Nepthea sp. The sample was collected from the waters of Saponda Island by SCUBA diving. It was extracted by ethyl acetate and fractionated using vacuum liquid chromatography. The chemical content was analyzed by phytochemical screening, LC-MS/MS analysis, Total Phenolics Content and Total Flavonoids Contents. Antioxidant potency was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and ABTS (2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid). Cytotoxicity property was analyzed by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. The result showed that the fractionation of Nepthea ethylacetate extracts produced six fractions (A-F). Fractions A and B contain non-polar compounds. Based on LC-MS/MS data, the non-polar compounds in Fraction A and B include achillin, atractylenolide II, buthyl isobuthyl phthalate, rengyolester, 2a-acetoxycostic acid, ocotillol acetate, petasitolone and some unidentified compounds that are C33H58O4, C15H21NO, C21H33NO, and C16H20O4. In general, the antioxidant and cytotoxic properties of all samples are in the weak category, however, when examined for each sample, the antioxidant properties of fraction B is slightly better than fraction A based on the IC50 value of DPPH and ABTS. Cytotoxicity of Fraction A is better than Fraction B against Breast Cancer cell lines MCF-7. The non-polar fraction of Nepthea sp. can be developed as raw material for the discovery of new compounds, antioxidant and anticancer agents, especially breast cancer.
Potensi Antibakteri Ekstrak Metanol dan Senyawa Aromatik dari Buah Wualae (Etlingera elatior) Sahidin Sahidin; Syefira Salsabila; Wahyuni Wahyuni; Fristiohady Adryan; Imran Imran
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 1, May 2019
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.334 KB) | DOI: 10.15408/jkv.v5i1.8658

Abstract

Buah tanaman wualae (bahasa Tolaki) atau Etlingera elatior digunakan oleh masyarakat Tolaki (Sulawesi Tenggara) sebagai bumbu masak dan obat tradisional. Untuk mengungkap potensi tersebut, khususnya buah wualae, dilakukan kajian fitokimia dan farmakologi buah wualae. Kajian fitokimia meliputi isolasi dan penentuan struktur dikerjakan dengan metode kromatografi (kromatografi vakum cair dan kromatografi radial) dan spektroskopi (1H dan 13C NMR). Kajian farmakologi meliputi uji aktivitas biologi terhadap enam jenis bakteri (Bacillus subtilis FNCC 0060, Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica ATCC 14028, Staphylococcus aureus ATCC 25923, dan Streptococcus mutans ATCC 25175). Hasilnya menunjukkan tiga senyawa fenolik telah berhasil diisolasi dan diidentifikasi dari buah wualae yaitu asam vanilat (1), asam p-hidroksibenzoat (2), dan asam kumarat (3). Ketiga senyawa tersebut pertama kali dilaporkan berhasil diisolasi dari buah E. elatior. Aktivitas antibakteri ekstrak metanol buah wualae dan tiga senyawa hasil isolasi menunjukkan bahwa sampel tersebut paling aktif terhadap bakteri S. mutans. Dengan demikian aktivitas senyawa-senyawa hasil isolasi dari buah E. elatior  berkorelasi dengan aktivitas ekstrak metanol buah E. elatior  terhadap S. mutans, dimana senyawa paling aktif adalah asam vanilat. Ekstrak yang dominan mengandung asam vanilat dari tanaman ini dapat dikembangkan sebagai herbal antibakteri S. mutans seperti herbal karies gigi dan obat kumur. Kata kunci: Etlingera elatior, wualae, buah, senyawa aromatik, antibakteri. The fruits of wualae (Tolakinese) or Etlingera elatior is utilized by Tolakinese (Sulawesi Tenggara) as spices and traditional medicine. To reveal the potencies, a study on phytochemical and pharmacological aspects have been carried out. The phytochemical study includes isolation and structure determination used chromatography methods (vacuum liquid chromatography and radial chromatography) and spectroscopy methods (1H NMR and 13C NMR). Biological activity was evaluated against some bacteria that are Bacillus subtilis FNCC 0060, Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica ATCC 14028, Staphylococcus aureus ATCC 25923, and Streptococcus mutans ATCC 25175). The results showed that three aromatic compounds had been isolated and identified that are vanillic acid (1), p-hydroxybenzoic acid (2) and coumaric acid (3). All compounds are firstly reported that successfully isolated from fruits of Wualae. Antibacterial potency indicated that the methanol extracts and the isolated compounds are the most active towards S. mutans and have good activity correlation between the extracts and the compounds. Vanillic acid is the most active compound from E. elatior fruits. Thus, the extracts contain dominantly vanillic acid from          E. elatior fruits can be developed to be antibacterial herbals caused by S. mutans. Keywords: Etlingera elatior, wualae, fruits, aromatic compounds, antibacterial activity.
RESVERATROL 10C-GLUKOPIRANOSIDA DAN HEIMIOLA, KARAKTER Hopea gregaria SECARAKEMOTAKSONOMI Sahidin Sahidin; EH Hakim; YM Syah; LD Juliawaty; SA Achmad; LB Din; J Latip
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.785

Abstract

Ten resveratrol oligomers have been isolated from the stem bark of H. gregaria i.e. resveratrol lOC-glucopyranoside (1), heimiol A (2), balanocarpol (3), ampelopsin A (4), hopeaphuran (5), E-viniferin (6), parviflorol (7), a-viniferin (8), hopeaphenol (9), and vaticanoi B (10). The structures of these compounds were determined base on spectroscopic methods including UV, IR, 1-D NMR, 2-D NMR and comparison with the reported data. Implication of the compounds invention is not only strengthen conclusion that Hopea tends to produce resveratrol dimmers but also showed that resveratrol lOC-glucopyranoside (1) and heimiol A (2) are phytochemical markers of. H. gregaria.
AKTIVITAS IMUNOSTIMULATOR EKSTRAK ETANOL BUAH Etlingera rubroloba A.D. POULSEN TERHADAP KADAR CD8 MODEL IN VIVO: IMMUNOSTIMULATORY ACTIVITY OF Etlingera rubroloba A.D. POULSEN FRUIT ETHANOL EXTRACT AGAINST CD8 LEVELS IN VIVO MODEL Muhammad Ilyas Y.; Ajeng Diantini; Mohammad Ghozali; I Sahidin; Wa Ode Nurfinti
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 6 No 2 (2021)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.745 KB) | DOI: 10.37874/ms.v6i2.284

Abstract

Pendekatan kemotaksonomi genus Etlingera memungkinkan Etlingera rubroloba A.D. Poulsen memiliki kandungan metabolit sekunder sebagai agen imunomodulator. Penelitian ini bertujuan mengetahui efek imunostimulator ekstrak etanol buah E. rubroloba A.D. Poulsen dengan parameter kadar CD8 (Cluster of differentiation) pada tikus jantan galur wistar. Hewan uji tikus sebanyak dua puluh empat ekor dibagi dalam enam kelompok perlakuan yaitu kontrol normal, kontrol pelarut (Na.CMC 0,5%), kontrol positif (ekstrak meniran komersil®), perlakuan ekstrak dosis 200, 300, 400 (mg/kgBB). Perlakuan diberikan selama tujuh hari berturut-turut secara per oral  dan pada hari kedelapan masing-masing kelompok diinfeksi bakteri Staphylococcus aureus 0,5 mL secara intraperitonial. Kadar CD8 diukur dengan metode ELISA dan data dianalisis dengan one way ANOVA. Hasil penelitian menunjukan perlakuan dosis ekstrak etanol buah E. rubroloba A.D. Poulsen memiliki efek imunostimulator berdasarkan kadar CD8 yang berbeda signifikan dengan kontrol pelarut (p<0,05), sehingga berpotensi dikembangkan sebagai imunomodulator alamiah.
Characteristic Test and Antihyperuricemia Activity of Ethanol Extract Stem of Etlingera rubroloba A.D Poulsen In vitro Asriullah Jabbar; Idin Sahidin; Khansa Rafida Triaqilah; Mubarak Mubarak; Muhammad Ilyas Yusuf
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 9, No 2 (2022)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46496/medula.v9i2.25290

Abstract

Background: Etlingera rubroloba (E. rubroloba) A.D Poulsen is one of the endemic plants of Southeast Sulawesi, which has the largest species in the world. Local people widely use this plant as traditional medicine. Many of these plant species have been previously reported as antioxidants, antibacterials, and others. However, for E. rubroloba, there are still very few reports from a pharmacological aspect, especially anthiperuricemia. Objective: This study was conducted to determine the characteristics and activity of the ethanol extract of the stem of E. rubroloba A.D Poulsen in vitro. Methods: The stems of E. rubroloba A.D Poulsen were extracted with ethanol solvent by maceration, then the filtrate obtained was evaporated using an evaporator to get an ethanol extract. Furthermore, the extract characterization test was carried out, including water content, ash content, water-soluble extract content, and ethanol-soluble extract content. Then the antihyperuricemia activity test in vitro using the Xanthine Oxidase (XO) kit. Results: The results of the characteristic test showed that the ethanolic extract of E. rubroloba contained 5.15% water content, 5.485% ash content, 55.2% water-soluble extract content, and 56.52% ethanol-soluble extract content. Furthermore, the antihyperuricemic activity of the ethanol extract of E. rubroloba was IC50 48.59 ± 0.03 g/mL and Allopurinol 42.21 ± 0.02 g/mL as positive controls. Conclusion: E. rubroloba stem ethanol extract has characterization results according to the literature, has antihyperuricemia activity, and is a reference for the development of traditional drugs
AKTIVITAS IMUNOMODULATOR EKSTRAK ETANOL SPONS Callyspongia sp. TERHADAP FAGOSITOSIS MAKROFAG PADA MENCIT JANTAN BALB/C. Muhammad Ilyas Y.; Asriullah Jabbar; Mentarri Bafadal; Muhammad Hajrul Malaka; Firdayanti Firdayanti; I. Sahidin
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 1 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.441 KB) | DOI: 10.36387/jiis.v5i1.377

Abstract

Sponge Callyspongia sp. has compounds that act as immunomodulatory agents, one of which is flavonoids.Flavonoids have the potential to be immunostimulants by increasing the phagocytic activity of macrophages. This study was experimental with a post test only control group design method. This study used 24 micemalestrain Balb /c with a body weight of 20-30 grams divided into 6 groups. The first group get extracts of sponge Callyspongia sp. 100 mg/kgBW, second group get the extract of sponge Callyspongia sp. 200 mg/kgBW, third group get extracts of sponge Callyspongia sp. 300 mg/kgBW, fourth group get the extract of sponge Callyspongia sp. 400 mg/kgBW, positive control group was given Stimuno® 0,13 mg/kgBW and the negative control group get Na-CMC 0.5%. A test given in orally preparations on the first day until the seven day. On the eighth day of each of the mice are injected bacteria Staphylococcus aureus (SA) by intraperitoneal. The activity of macrophage cells from murine peritoneal fluids sweep by calculating the percentage of macrophages that does phagocytosis of 100 cells are observed. The data obtained were then analyzed by one way Anova statistics and test post hoc Tukey's. As result, the are that ethanol extracts of sponge Callyspongia sp. at doses of 300 mg/kgBW and 400 mg/kgBW have potential as immunomodulator with based on results of statistical tests post hoc Tukey (P > 0.05) showed no meaningful difference against the positive control (Stimuno ®) wich improving the activity of the cells of the macrophage phagocytosis.
Penguatan peran warga masyarakat dalam mitigasi dan adaptasi menghadapi wabah Covid-19 di Kabupaten Kolaka Sudarman Rahman; Sahidin Sahidin
INDRA: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 1 (2021): April
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.771 KB) | DOI: 10.29303/indra.v2i1.51

Abstract

WHO China Country Office reported pneumonia cases in Wuhan, Hubei Province, China. The virus was lately identified as a new strain of corona virus called Severe Acute Respiratory Syndrome Coronavirus-2. The new cases grow massively and became pandemic. Regarding this rapid transmission, there is a need for socialization to strengthen people’s awareness and role in mitigation and adaptation against this  COVID-19 pandemic. The method used was counseling with socialization or lectures and supported interactions in the form of questions and answers by using power points and leaflets/brochures as information media. This community engagement included the activities of the Movement for Healthy Living Society (GERMAS), Clean and Healthy Living Behavior (PHBS), Use of Personal Protective Equipment such as using masks, Use of Antiseptics and Disinfectants, and Planting Family Medicines (TOGA). The implementation of this activity began with socialization to the local community at the village hall, then to the houses of residents to deliver the subject contained in the brochure/leaflet, and last by placing pamphlets in public places to provide information to the community related to the activities that had been done. This socialization is expected to increase public awareness to face against this corona virus disease outbreak.
Aktivitas Antibakteri Ekstrak Etanol Buah Wualae (Etlingera elatior (JACK) R.M. Smith) Wahyuni Wahyuni; Fadhliyah Malik; Andryani Ningsih; Wa Ode Sitti Zubaydah; Sahidin Sahidin
Jurnal Farmasi dan Ilmu Pengobatan Vol 3 No 1 (2018): JPMS
Publisher : STIFA Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.707 KB)

Abstract

Wualae (Etlingera elatior (Jack) R.M. Smith) belonging to Zingeberaceae that spread in South East Sulawesi, empirically used as staple and treatment. The aim of this research to determine the antimicrobial potential of ethanol extract fruit of E. elatior against gram positive (Staphylococcus aureus ATCC 25923 and Bacillus subtilis FNCC 0060), and gram negative (Pseudomonas aeruginosa ATCC 27853 and Salmonella enterica ATCC 14028) using cup-plate method. The antimicrobial activity based on the diameter of inhibition zone. The result shows that extract more potential to inhibit gram positive than gram negative while the highest inhibition illustrate by concentration of 20 mg/mL (inhibition zone= 5 mm). In conclusion, the extract of E. elatior no potent as antimicrobial candidate (category= weak).
Nephroprotective effect of extract Etlingera elatior (Jack) R.M. Smith on CCl4-induced nephrotoxicity in rats Adryan Fristiohady; Wahyuni Wahyuni; Muhammad Ilyas Y; Mentarry Bafadal; La Ode Muhammad Julian Purnama; Firasmi Sangadji; Muhammad Hajrul Malaka; Fadhliyah Malik; Junli Hong; Agung Wibawa Mahatva Yodha; I Sahidin
Current Research on Bioscences and Biotechnology Vol. 1 No. 2 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/10.5614/crbb.2019.1.2/DAPM6106

Abstract

Reactive oxidative stress (ROS) can lead to cell damage, and one of them is the kidney's cell. Etlingera elatior (Jack) R.M. Smith can be utilized as an agent that can protect the cell from ROS. This study aimed to investigate the protective effect of E. elatior fruit on the kidney's cell. We used experimental animals which were treated with Na-CMC (Group I), Na-CMC (Group II), the extracts of E. elatior fruit 200, 300, 400 mg/kg BW for Group III, IV, and V, respectively, for seven days. The blood was collected after treatment. At day 8, group I, III, IV, and V were induced by CCl4. At day 9, blood was collected and the kidneys were harvested for histopathology analysis. Blood collected were measured for albumin, total protein, urea and creatinine levels. After treatment, albumin and total protein showed no increased levels; urea decreased at doses of 200, 300, and 400 mg/kg BW, respectively, and creatinine levels only decreased at the dose of 400 mg/kg BW (p<0.05). The dose of 200 and 300 mg/kg BW showed protecting effects in the tubular cells of renal. Therefore, the ethanol extract of E. elatior showed a nephroprotective effect by normalizing the urea and creatinine levels of rats and protecting tubular cells of renal.
Co-Authors ,, Suryani A.A. Ketut Agung Cahyawan W Abdi Nipangeran Abdul Fajar La Ode Abdul Karim Abdul Khoir HS Abdulkadir Kamaluddin Achmad, SA Ade Dedi Rohayana Adriatman Rasak ADRYAN FRISTIOHADY Adryan Fristiohadi Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiyohadi Agung Wibawa Mahatva Yodha Agustina Agustina Ahmad Nursodiq Ahmad Zaeni Ajeng Diantini, Ajeng Alyza Zaulya Amalia Alfitrianingrum Amalia, Riezki Amaliah Amaliah Amaliah, Wa Ode Fitri Amir Tajrid Ananda, M Aflahmuna Anas Subarnas Andi Septiana Andini Sundowo Andini Sundowo Andini Sundowo Andini Sundowo Andini Sundowo Andriani, Rina Andryani Ningsih Angriani Fusvita Annisa Fatinah Gani Arba, Muh. Arba, Muhammad ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Arfan Arfan Arfan Arfan Arfan ARFAN ARFAN Ari Sartinah Ari Sartinah, Ari Aries Saifudin Arif Rahman Hakim Asasutjarit, Rathapon Asman Sadino Asman Sadino Asman Sadino ASMAN SADINO, ASMAN Asmawati Asmawati ASNANI ASNANI Asniar Pascayantri Asniar Pascayantri Asnita Hafsani Aspadiah, Vica Asriullah Jabbar Asriullah Jabbar Asriullah Jabbar Asriullah Jabbar Asriullah Jabbar Asrul Sani Asrul Sani, Asrul Asrun Budiatma Astri Alawia Astrid Indalifiany Atasya, Fretiniar Ayu Wulandari Badia, Esti Bafadal, Mentarry Baru Sadarun Baru Sadarun Baru Sadarun Baru Sadarun Baso, La Bonni Rubak Budikafa, Muhammad Jefriyanto Cicilia, Retno Daud, Nur Saadah Dayatriana Muthalib Dewi Puspita Dewiyanti Madu Dian Munasari Diasty Nuraisya Din, LB Dzaky Aulia Rahman EH Hakim Elfahmi Elfahmi, Elfahmi Euis H. Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Julaeha Fadeli Ehsan Hanafi Fadhliyah Malik Fahria Nadiryati Sadimantara Fahria Nadiryati Sadimantara Fahriah Nadiryati Sadimantara Faisal Faisal Fajri Fitra Amalia FANNY RAHMADHANI .N Fathan Ramadhan Fatima, Nur Fauzia Azzahra, Fauzia Fauziah, Yulianti Febrianti, Sari Firasmi Sangadji Firdayanti Firdayanti Firdayanti, Firdayanti Firman Oktivendra Fitrawan, La Ode Muhammad Fitrawan, La Ode Muhammad Fredy Talebong Frely Cristiadzi Fawaz Fristiohady Adryan Fristiohady, Adryan Galih, Olga Grasiana Eka E.Y.G Gunawan, Syahrul Gusti Ray Sadimantara Gusti Ray Sadimantara Gusti Ray Sadimantara Hadad, Nur Diana Haera, Evi Hadrawati Hair, La Hajah Ningsih Inta Hakim, EH Hakini, EuisH Halik, Halik Hamsah, Hamsah Handayani, Lili Sri HANIFAH, SYIFA Hariana Haristan, Haristan Harnita, Wa Ode Hartina Hartina Haruna, Lidya A. Haruna, Lidya Agriningsih HASLIANTI HASLIANTI Hasnawati Hasnawati Hasnawati Hasriani H Japar Hastiar, Arfiani Helmia, Wa Ode Nia Hendra Febriansyah Hendrawan Tulungi, Wahyu Hikmayani I W. Sutapa Ibnu Nurrahman, Pandu Idhul Ade Rikit Fitra Idrus, Loly Subhiaty Ilhan Fauzan Ilma Sarimustaqiyma Rianse Ilyas Y, Muhammad Ilyas Y., Muhammad Imam Yahya Imran Imran Imran Indalifiany, Astrid Intan Permata Putri Iren Meylani Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irvan Anwar Izzati, Emy J Latip Jabbar, Asriullah Jalifah Latip Jalifah Latip Jamili Jamili Japar, Hasriani H. Juliawaty, LD Juliawaty, Lia D Jumadil Jumriani Jumriani Junli Hong Kadidae La Ode Kamaluddin Kamaluddin Kangkuso Analuddin Kangkuso Analuddin, Kangkuso Kardin, Laode Karimu, Laila Qodriyah Karmilah, Karmilah Khansa Rafida Triaqilah Kholilah, Aulia Kirana, Rezky Marwah Kuncoro, Shienny L. O. A. N. Ramadhan La Ode Ahmad La Ode MJ Purnama La Ode Muh. Fitrawan La Ode Muh. Julian Purnama La Ode Muhammad Andi Zulbayu La Ode Muhammad Julian Purnama La Ode Muhammad Julian Purnama La Ode Muhammad Julian Purnama La OMJ. Permana Laily Bin Din Laily Bin Din Laode Muhamad Mbuyang Nadia Latip, J LB Din LD Juliawaty Lia D. Juliawaty Lia Dewi Juliawaty Lidya Agriningsih Haruna Lidya Agriningsih Haruna Loly Subhiaty Idrus Loly Subhiaty Idrus Lubis, Adryan Fristiohady M. Hajrul Malaka Malaka, Muh. Hajrul Malaka, Muhammad Hajrul Malaka, Muhammad Hajrul Manggau Marianti Mangurana, Wa Ode Intiyani Mardikasari, Sandra Aulia Marianti A Manggau Marianti A. Manggau Marianti, Manggau Martiani Martiani Maryani Masrika, Nur Upik En Maulana Anwar Mawaddah, Nur Ainul Mega Fatimah Rosana Mentarri Bafadal Mesi Leorita Mesi Leorita Mesi Leorita, Mesi Mini Bekti Ningsih Mirawanti G, Wa Ode Mirda Mirda Mirdayani, Eli Mochammad Imron Awalludin Mohammad Ghozali Muamar Abdillah Mubarak Mubarak Mugiarno, Dita Oktavianti Muh. Arba Muh. Ilyas Yusuf Muhamad Handoyo Sahumena Muhamad Handoyo Sahumena Muhammad Alim Marhadi Muhammad Arba Muhammad Arba Muhammad Azhar Muhammad H Malaka MUHAMMAD HAJRUL MALAKA Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Ilyas Muhammad Ilyas Y Muhammad Ilyas Y Muhammad Ilyas Y Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Israwan Azis Muhammad Isrul Muhammad Ramadhan Muhammad Ramli Muhammad Rifky Wahyu Alfatih Muhammad Yasir Haya La Ode Muhammad, Taher Muktiarni, Muktiarni Mursanti, Wa Ode Indah Arum Musadar Mappasomba Musdalifa Musdalifa Musdalipah MUSDALIPAH MUSDALIPAH Mustakim Muzuni, Muzuni N. Nohong Nadaoka Kazuo Nadhifatul Aslikah Nasir Nasir Nasir Nasrudin NASRUDIN Nasrudin Nasrudin Nasution, Ali Napiah Nazira, Arni Neneng Andriani Joko Ni Kadek Ayu Berlian Nirwati Rusli Nofriani, Andi Nohong Nohong Nohong, Nohong Nordin Hj Lajis Nubzatussaniyyah, Nunu Nugroho, Ariel Dwiyanto Nunuk Hariani Soekamto Nur Aisyah Abdullah Nur Fadhillah Sahari Sahari Nur Illiyyin Akib Nur Indri Rahayu Nur Salma Nur Syifa Rahmatika Nur Syifa Rahmatika Nur Syifa Rahmatika Nur Syifa Rahmatika Nurcahyani, Fitria Nurhikma Nurhikma Nurhikma Nurkhalis Bintang Astiasal Nurpajriani, Nurpajriani Nurramadhani A. Sida Nurull Hikmah Ode, Muhammad Fajrin Parawansah Parawansah Parawansah Parrung, Arsyani Pascayantri, Asniar Paulina Taba Pauzan Haryono Poko, Feny Riskiana Poko, Feny Rizkia Purnama, La O. M. J. Purnama, La Ode Muh. Julian Purnama, La Ode Muhammad Julian Pusmarani, Jastria Puspita Sari Putri, Natalia Christiani Putri, Olivia Dicha Raden Arfan Rifqiawan Raden Maya Febrianti Rahangga, Dewa Gede Oka Rahman, Sudarman RAHMAT MULIADI, RAHMAT Rahmatika, Nur S. Ramadhan, LOAN Ramadhani, Rizky Barkah Rathapon Asasutjarit Rathapon Asasutjarit Reymon, Reymon RH. Fitri Faradilla Riezki Amalia Rilla Dwi Anjasari Mangidi Rina Andriani Rina Andriani Rina Andriani Rina Andryani Rina Maryanti RINI HAMSIDI Rini Hamsid Rini Hamsidi Roihan Ramadhan, Muhammad Romadhon Haris Alfian Ruslan Ruslin Ruslin Ruslin Ruslin Ruslin Ruslin, Ruslin Rustam HN, Muhammad S. J. Suprapto SA Achmad Saban Rahim Sabarwati, Hadijah Sadimantara, Gusti Ray SADIMANTARA, I GUSTI RAY Saharuddin Sahputra, Adi Salsabila, Syefira Saputri, Ulan Mulyani Saranani, Muhammad Aqsal Azriel Fajar Saripuddin Saripuddin Saripuddin Saripuddin Saripuddin Sariyana, S Sasmita, Wa Ode Indri Selfyana Austin Tee Septiana Andi Sernita Setiawan, La Ode Roni Setiawan, La Ode Wawan Setiawan, Muhammad Azdar Sewan Theeramunkong Shiddiq, Asep Mohamad I Siti Helmyati Siti Rahmawati Ondjo Siti Suriyani Kapa Siti Syafika Sitti Mardiana Sitti Rahmawati Sitti Raodah Nurul Jannah Sitti Raodah Nurul Jannah Sjamsul A. Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sofa Fajriah Sofa Fajriah Sofa Fajriah Solo, Dian Munasari Sri Hutami Lety Grashella Srimuliana Suaib, S Sulsiah Sulsiah Sunandar Ihsan Supangat Supangat Suryani Suryani Syah, YM Syahrir La Syarif, Aiman Syarul Nataqain Baharum Syarul Nataqain Baharum Syukur, Novita Sari Taher Muhammad Tanzil, Dwi Lestari Khaulani Tatik Handayani, Tatik Thamrin Azis Tian Amalia Halik Tien Tien Titin Syafiransyah Trinovitasari, Nita Trisnaputri, Dian Rahmaniar Ubaidillah W Wahyuni, W W. O. N. Haryanti W. Wahyuni WA KUASA Wa Ode Erna Fitriani Wa Ode Ida Fitriah Wa Ode Jumiati Nur Wa Ode Mutiara Wa Ode Norma Wa Ode Nurfinti Wa Ode Nurfinti Wa Ode Salma Wa Ode Siti Zubaydah Wa Ode Sitti Zubaydah Wa OIL. Kalimin WAHYUNI Wahyuni Wahyuni wahyuni wahyuni Wirasmanto, Bayu Wirhamsah Al-Ramadan Wiwik Rusdin wiwin Wiwin Y, Muhammad Ilyas Yamin Yamin Yana M Syah Yana M. Syah Yana Maolana Syah Yayan Sopyan Yerin Tri Agustina YM Syah Yuliansyah Yuliansyah Yulianti, Sri Samrina Yuliastri, Wa Ode Yunarzat, Ersyanda Yuni Elsa Hadisaputri Yusnaini Yusnaini Zubaydah, Wa Ode Sitti Zulbayu, La Ode Muhammad Andi