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Antibacterial and antioxidant potentials of methanol extract and secondary metabolites from Wualae rhizome (Etlingera elatior) Wahyuni; Sri Hutami Lety Grashella; Wa Ode Ida Fitriah; Muhammad Hajrul Malaka; Adryan Fristiohady; Imran; Sahidin
Current Research on Bioscences and Biotechnology Vol. 1 No. 1 (2019)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2019.1.1/IHHZ547

Abstract

The rhizome of Etlingera elatior or Wualae (Tolakinese) has many advantages on traditional remedies and cooking in Sulawesi Tenggara. To support those advantages, two secondary metabolites derived from steroid and phenylpropanoic acid classes, stigmast-4-en-6β-ol-3-one (1) and p-coumaric acid (2), respectively, have been firstly isolated and identified from the E. elatior rhizome. Isolation of these two compounds was performed using several chromatography techniques, including thin layer chromatography (TLC), vacuum liquid chromatography (VLC) and radial chromatography (RC). Identification of isolates was carried out using 1 H and 13C NMR spectroscopy and comparing the spectroscopy data with the library. The potency of antibacterial of the methanol extract of Wualae rhizome and the isolates were evaluated against Pseudomonas aeruginosa ATCC 27853, Bacillus subtilis FNCC 0060, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Salmonella enterica ATCC 14028, and Streptococcus mutans ATCC 25175 using agar diffusion method. Antioxidant activity was evaluated against DPPH radicals (2,2-diphenyl-1-picrylhydrazyl). The results show that the antibacterial potential of Wualae methanol extract is better than compound 1 and 2. Furthermore, the antioxidant properties of Compound 2 (IC50 159.47 μg/mL) was stronger than the antioxidant properties of Compound 1 (IC50 219.95 μg/mL) and the methanol extract (IC50 586.38 μg/mL).
Acute Toxicity of Ethanol Extract of Polygonum pulchrum Blume using Brine Shrimp Lethality Test Method Asman Sadino; Idin Sahidin; Wahyuni Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 2, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.058 KB) | DOI: 10.15416/pcpr.v2i2.15210

Abstract

The potential toxicity effect presents in the medicinal plants is important to be identified forthe safety assurance. Accute toxicity study is an initial step in the drug safety assurance test.Thus, the aim of this study is to identify the potential acute toxicity of roots, stems, leaves,and flowers ethanol extracts of bamboo bamboo plants (Polygonum pulchrum Blume). Thisstudy used brine shrimp lethality test (BSLT) method. The total number of larvae used ineach concentrations in three times replications was 330 larvae. Each group was given consecutivelyroots, stems, leaves and flowers ethanol extracts of P. pulchrum Blume plants withvariation concentrations 12.5 μg/ml, 25 μg/ml, 50 μg/ml, 100 μg/ml, 125 μg/ml, 250 μg/ml, 500 μg/ml, 1000 μg/ml, 2000 μg/ml, and 4000 μg/ml. The number of dead larvae after24 hours treatment were calculated to obtain the mortality percentages and to determine theLC50 value, which were determined by probit analysis using MiniTab application version17.1.0. Ethanol extract of P. pulchrum roots and stems resulted LC50 values of 933.08 μg/mland 919.58 μg/ml, respectively. While the LC50 values of leaves and flowers extracts were2207.06 μg/ml and 1081.90 μg/ml, respectively. In conclusion, P. pulchrum Blume roots andstems were classified as toxic according to BLST method, while leaves and flowers were not.Keywords: acute toxicity, brine shrimp lethality test, Polygonum pulchrum Blume
Anti-Inflammatory Activity of Marine Sponge Aaptos sp. to the Plasma Interleukin-1β Level in Wistar Male Rats Adryan Fristiyohadi; Wahyuni Wahyuni; Wa OIL. Kalimin; La OMJ. Permana; Saripuddin Saripuddin; Idin Sahidin
Pharmacology and Clinical Pharmacy Research Vol 4, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.584 KB) | DOI: 10.15416/pcpr.v4i2.24269

Abstract

Inflammation is the response of the body to injury and infection characterized by swelling, heat, pain, and redness. This study aimed to investigate the anti-inflammatory effect of Aaptos sp. ethanolic extract to plasma interleukin (IL)-1β level of Wistar male rats. Aaptos sp. was macerated with 96% ethanol for 3 x 24 hours. Inflammation was induced with administration of 1% carrageenan intraplantarly. Animals were divided into 5 treatment groups, i.e., positive control  (diclofenac sodium 3598 ppm); Aaptos sp extract 50 ppm; Aaptos sp extract 100 ppm Aaptos sp extract 200 ppm; and negative control (0.5% Na CMC). After 1 hour, blood was collected and assayed using enzyme-linked immunosorbent assay (ELISA) kit. The results showed that plasma IL-1β levels of animals were decreased by Aaptos sp ethanolic extract. The administration of 50 ppm of extract showed no significant difference (p>0.05)  in IL-1β level in first and second hour measurement, but indicated a statistically significant decrease after three hour (p<0.05). The administration of 100 ppm of extract showed no significant difference (p>0.05) in every hour. Significant reduction was observed in the administration of 200 ppm of extract, but the elevation of IL-1β levels was also observed at third hour measurement. In conclusion, ethanolic extract of Aaptos sp. had anti-inflammatory activity and its effective dose was 50 ppm.   
Antibacterial Activity of Polygonum pulchrum Blume Ethanol Extract on Staphylococcus aureus and Escherichia coli Asman Sadino; Idin Sahidin; Wahyuni Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 3, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.919 KB) | DOI: 10.15416/pcpr.v3i2.18097

Abstract

The emergence of resistant bacteria strain has become a global health concern. It encourages the exploration of potential antibacterial agents, particularly from natural sources. The aim of this study was to investigate the antibacterial activity of ethanol extract of root, stems, leaves, and flowers of Polygonum pulchrum Blume against Staphylococcus aureus and Escherichia coli, through disc diffusion method using cup-plate method. Inhibition zone against S. aureus from roots, stems, leaves, and flowers ethanol extract were 3.5 mm, 2.5 mm, 2.25 mm, and 2.62 mm, respectively, while the inhibition zone against E. coli were 2.25 mm, 2.12 mm, 1.62 mm, and 1.75 mm, respectively. In conclusion, ethanol extract of root, stem, leaves, and flower of P. pulchrum Bl possessed weak antibacterial activity against S. aureus and E. coli.Keywords: P. pulchrum Bl, antibacterial, E. coli, S. aureus, cup-plate technique
Antioxidant Activity of Ethanol Extract of Polygonum pulchrum Blume Asman Sadino; Idin Sahidin; Wahyuni Wahyuni
Pharmacology and Clinical Pharmacy Research Vol 1, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.767 KB) | DOI: 10.15416/pcpr.v1i2.15201

Abstract

The use of antioxidants from natural resources has attracted increasing interest. One of theplant that was empirically used as an antioxidant dietary supplement was Polygonum pulchrumBlume (P. pulchrum Blume). This study aimed to investigate antioxidant activityof roots, stems, leaves and flowers ethanol extract of P. pulchrum Blume. The extract wasobtained by maceration method using ethanol solvent. Antioxidant activity was determinedwith 1,1-diphenyl-picrylhydrazyl (DPPH) method. We found that ethanol extracts of P. pulchrumBlume roots and stems had strong antioxidant activity with IC50 values of 25.2 mg/land 43.26 mg/l, respectively. Ethanol extract of flowers had the weakest antioxidant activitywith IC50 value of 202.96 mg/l. Vitamin C had very strong antioxidant activity with IC50value of 3.97 mg/l. In conclusion, our study revealed that ethanol extract of Polygonumpulchrum Blume roots and stems had strong antioxidant activity. Therefore, P. pulchrumBlume might be potential as an excellent source for natural antioxidant agents for medicalapplication.Keywords: Polygonum pulchrum Blume, DPPH, antioxidant, maceration, ethanol
Ethanolic Extract of Xestospongia Sp. Induces CD4+ and CD14 Cells Levels on Wistar Male Rat Infected with Staphylococcus aureus Adryan Fristiohady; Wahyuni Wahyuni; Muhammad H Malaka; Dewiyanti Madu; Dayatriana Muthalib; Dian Munasari; La Ode MJ Purnama; Baru Sadarun; Muhammad Ilyas; Idin Sahidin
Pharmacology and Clinical Pharmacy Research Vol 5, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15416/pcpr.v5i2.26986

Abstract

Immunomodulator is a substance that increases or suppresses the immune response through the certain mechanism. The marine sponge Xestospongia sp. has immunomodulatory activity by increasing phagocytic activity. In addition, the phagocytic activity is affected by CD4+ and CD14 cells levels. Thus, this study aims to investigate the effect of Xestopongia sp. extract toward CD4+ and CD14 cells level in model rat. Animals were divided into 4 groups (n=5) and treated for 7 days, as follow: Group I (Ethanolic extract of Xestospongia sp. dose of 300 mg/KgBW); Group II (Ethanolic extract of Xestospongia sp. dose of 400 mg/KgBW); Group III (Phylantus niruri extract); and Group IV (0.5% Na CMC). On day 8, animals were infected with Staphylococcus aureus intraperitoneally. And the blood was collected by cardiac puncture and assayed with ELISA kit CD4 (elabscience®) and ELISA kit CD14 (elabscience®). Ethanolic extract of Xestospongia sp. provided high levels of both CD4+ and CD14 cells (Group II) compared to baseline (Group IV)  (p<0.05). Group I provided similar activity to group III (p>0.05) and Group II provided significant activity with higher levels of CD4+ and CD14 cells compared to group III (p<0.05). In conclusion, both doses of Xestospongia sp. extract provide activity as immunomodulator by increasing CD4+ and CD14 cells levels, yet dose of 400 mg/KgBw provides the higher immunomodulatory activity.
Efek Imunomodulator Ekstrak Etanol Spons Xestospongia Sp. Terhadap Aktivitas Fagositosis Makrofag Pada Mencit Jantan Galur Balb/C Adryan Fristiohady; Wahyuni Wahyuni; Fadhliyah Malik; Mesi Leorita; Muhammad Ilyas Yusuf; Hendra Febriansyah; Sahidin Sahidin
Jurnal Mandala Pharmacon Indonesia Vol. 5 No. 01 (2019): Jurnal Mandala Pharmacon Indonesia
Publisher : Program Studi Farmasi STIKES Mandala Waluya Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v5i01.38

Abstract

Imunomodulator merupakan bahan yang dapat mengembalikan ketidakseimbangan sistem imun. Spons Xestospongia Sp. diduga mengandung senyawa-senyawa aktif yang berperan sebagai agen imunomodulator. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian ekstrak etanol Spons Xestospongia Sp. terhadap aktivitas fagositosis makrofag. Sebanyak dua puluh empat ekor mencit jantan galur Balb/C umur 2-3 bulan dengan berat badan 20-30 gram dibagi ke dalam 6 kelompok. Kelompok pertama mendapat pemberian ekstrak etanol Spons Xestospongia Sp. 100 mg/kgBB, kelompok kedua mendapat pemberian ekstrak etanol Spons Xestospongia Sp. 200 mg/kgBB, kelompok ketiga mendapat pemberian ekstrak etanol Spons Xestospongia Sp. 300 mg/kgBB dan kelompok keempat mendapat pemberian ekstrak etanol Spons Xestospongia Sp. 400 mg/kgBB. Kelompok kontrol positif mendapat ekstrak Phyllanthus niruri Linn. (Stimuno®) 0,13 mg/gBB dan kelompok kontrol negatif mendapatkan Na-CMC 0,5%. Ekstrak diberikan secara peroral sejak hari pertama hingga ketujuh. Pada hari kedelapan masing-masing mencit diinjeksikan bakteri Staphylococcus aureus (SA) 0,5 mL secara intra peritoneal. Aktivitas sel makrofag dihitung dari apusan cairan peritoneum mencit. Peningkatan dosis ekstrak etanol Spons Xestospongia Sp. meningkatkan jumlah aktivitas fagositosis makrofag dari 24,25 % (Na-CMC), 34,25% (100 mg/kgBB), 47,00% (200 mg/kgBB), 59,50 % (300 mg/kgBB) dan 62,75% (400 mg/kgBB). Hasil penelitian menunjukan bahwa ekstrak etanol Spons Xestospongia Sp. memiliki potensi sebagai imunomodulator pada dosis 300 mg/kgBB dan 400 mg/kgBB dengan efektivitas yang tidak berbeda bermakna dengan kontrol positif (Stimuno®) dalam meningkatkan aktivitas fagositosis sel makrofag berdasarkan hasil uji statistik post hoc TUKEY (sig. > 0,05).
UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BATANG BAMBU-BAMBU (Polygonum pulchrum) PADA MENCIT (Mus musculus) GALUR BALB/C YANG DIINDUKSI STREPTOZOTOCIN (STZ) Muhammad Ilyas Y; Wahyuni Wahyuni; Sahidin Sahidin; Amaliah Amaliah; Parawansah
BORNEO JOURNAL OF PHARMASCIENTECH Vol 3 No 1 (2019): Borneo Journal of Pharmascientech
Publisher : Sekolah Tinggi Ilmu Kesehatan Borneo Lestari Banjarbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Batang bambu-bambu (Polygonum pulchrum) adalah salah satu tanaman yang berkhasiat sebagai antidiabetik dimana kandungan kimia batang bambu-bambu yang berpotensi sebagai antidiabetik antara lain flavonoid dan fitosterol. Fitosterol berkhasiat untuk meluruhkan kencing (diuretik) dan menurunkan kadar glukosa darah (hipoglikemik), karena peran senyawa aktif diantaranya β-sitosterol dan stigmasterol dan senyawa flavonoid berperan secara signifikan meningkatkan aktivitas enzim antioksidan dan mampu meregenerasi sel-sel β-pankreas yang rusak sehingga defisiensi insulin dapat diatasi juga serta dapat memperbaiki sensitifitas reseptor insulin. Penelitian ini bertujuan untuk mengetahui efek ekstrak batang bambu-bambu terhadap antidiabetik pada mencit (Mus musculus) Galur Balb/C yang diinduksi Streptozotocin. Pada penelitian ini merupakan penelitian eksperimen dengan menggunakan metode RAL (Rancagan Acak Lengkap) dimana digunakan 20 ekor mencit dibuat model diabetes dengan menggunakan streptozotocin 150 mg/kgBB secara intraperitonial. Kemudaian dibagi menjadi 5 kelompok perlakuan, yaitu: Kontrol negatif Na.CMC 0,5%, kontrol positif glibenklamide 5 mg, ekstrak dosis 250 mg/kgBB, 350 mg/kgBB, 450 mg/kgBB. Hasil analisis statistik menunjukkan aktivitas antidiabetes pada mencit Balb/C yang diinduksi STZ dengan Fhit>Ftabel0.05 = 12.531>3.06 berpengaruh secara signifikan pada taraf kepercayaan 0.05 dimana ketiga dosis ekstrak memiliki efektivitas sebagai antidiabetes jika dibandingkan dengan kelompok kontrol positif (glibenklamid). Kata Kunci : Ekstrak batang bambu-bambu, antidiabetik, streptozotocin, Mencit Balb/C, glukosa dara ABSTRACT Stems of bambu-bambu (Polygonum pulchrum) is one of the plants are useful as anti diabetic which the chemical content of the bambu-bambu stems potentially as anti diabetic among others Flavonoids and Phytosterols. Fitosterol using to Shed urine (diuretic) and lower the blood glucose (hypoglycemic, because the role of active compounds such as β-sitosterol and stigmasterol and flavonoid compounds play a significant role in increasing antioxidant enzyme activity and able to regenerate damaged β-pancreatic cells so that, its creates insulin deficiency which can be overcome as well as to improve insulin receptor sensitivity. This study aimed to able to know the effect of antidiabetic in bambu-bambu stems extract toward mice (Mus musculus) Babl/C who inducted by streptozotocin (STZ). This study is kind of experimental study and using complete random design which is using twenty of mice as a diabetic model and its using streptozotocin 150 mg/kgBB intraperitonially. Then, it will be divided in to five groups of treatment namely negative control Na.CMC 0.5%, positive control glibenclamide 5 mg, extract dose 250 mg/kgBB, 350 mg/kgBB, and 450 mg/kgBB. The result of analysis statistics showing the lowering aktivity of antidiabetic at Fhit > F table0. 05 = 12.531>3.06 influential as significant 0,05. Then all three doses of extract have antidiabetic effectiveness when compared to the positive control group (glibenclamide). Keywords: Polygonum pulchrum stems extract, antidiabetic, streptozotocin, mice Balb/C, blood glucose.
Compounds Profile of Polar Subfraction of Ethyl Acetate Fraction of Soft Coral Nepthea sp., Antioxidant and Cytotoxic Properties Baru Sadarun; Adryan Fristiohady; Nur Syifa Rahmatika; Agung Wibawa Mahatva Yodha; Muhammad Hajrul Malaka; Andini Sundowo; I. Sahidin
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2103

Abstract

Nepthea sp., a soft coral In Indonesia's South-East Sulawesi, it thrives. The chemical and pharmacological properties of this genus have not been studied in this location. As a result, the goal of this page is to offer a broad overview of both properties of Nepthea sp. The sample was taken from Hoga Island. Methanol was used to extract the material, which was then fractionated with hexane and ethyl acetate to produce n-hexane, ethyl acetate, and methanol fractions. Vacuum liquid chromatography was also used to fractionate the ethyl acetate fraction. Phytochemical screening, LC-MS/MS, Total Phenolics Content (TPC), and Total Flavonoids Content (TFC) were used to determine the chemical contents. DPPH radicals and ABTS were used to assess antioxidant activity and MTT assays for cytotoxicity. Six subfractions are produced by fractioning the ethyl acetate fraction (160 g), with the most polar subfraction (F) weighing 15.4 g. The phenolics and flavonoids constituents in Subfraction F have IC50 values of 68.77±4.8 mg GAE/g extract and 0.22±0.1 mgQE/g extract, respectively. The presence of phenolics, flavonoids, and alkaloids in the subfraction F is supported by the LC-MS/MS data, Subfraction F has 3-ter-butyl-4-methoxyphenol (terpenoids or phenolics compound), isosalsoline (alkaloids), valine (amin acid), and several unidentified chemicals with molecular formulas C15H23NO3, C33H47NO4, C29H49NO3 (alkaloids or amino acid) and C8H18O3 (phenolic compound).The IC50 values (mg/L) of 101.1 ± 8.8 (DPPH) and 89.76 ± 7.5 (ABTS) indicate that F has antioxidant capacity, and it is also cytotoxic against breast cancer cell lines (MCF-7) with an IC50 of 170.72±6.5mg/mL.
ANALISIS LC-MS/MS (Liquid Crhomatogaph Mass Spectrometry) DAN METABOLIT SEKUNDER SERTA POTENSI ANTIBAKTERI EKSTRAK n-HEKSANA SPONS Callyspongia aerizusa YANG DIAMBIL PADA KONDISI TUTUPAN TERUMBU KARANG YANG BERBEDA DI PERAIRAN TELUK STARING Wa Ode Intiyani Mangurana; Yusnaini Yusnaini; Sahidin Sahidin
Jurnal Biologi Tropis Vol. 19 No. 2 (2019): Juli - Desember
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.182 KB) | DOI: 10.29303/jbt.v19i2.1126

Abstract

Abstrak : Spons merupakan bagian dari biota komponen penyusun ekosistem terumbu karang yang memiliki kandungan bioaktif. Salah satu jenis spons yang memiliki kandungan bioaktif adalah C. aerizusa. Tujuan dari penelitian ini adalah untuk melihat perbedaan kandungan bioaktif pada kondisi terumbu karang yang berbeda. Metode yang digunakan dalam survei kondisi terumbu karang dan penentuan stasiun pengamatan menggunakan metode Point Intercept Transect (PIT). Pemeriksaan metabolit sekunder menggunakkan metode Kromatografi lapis tipis menurut Harborne (1987), perbedaan kandungan senyawanya menggunakan analisis LC-MS/MS, pengujian aktivitas antibakteri menggunakkan metode sumuran. Hasil penelitian kondisi tutupan karang menunjukan bahwa kondisi tutupan karang hidup 19-65% dengan kategori buruk hingga baik. Jumlah senyawa pada stasiun I mencapai 15 dan jumlah senyawa pada stasiun II mencapai 13. Kandungan Metabolit Sekunder untuk C. aerizusa dari kedua stasiun sama, yaitu aktif terhadap alkoloid, steroid, flavonoid, terpenoid, dan saponin. Potensi Antibakteri Ekstrak n-heksana Spons C. aerizusa untuk stasiun I dan II tidak aktif terhadap E. coli namun Spons C. aerizusa stasiun I dan II aktif terhadap aktivitas S. mutans. Kata Kunci :  n-heksana, Terumbu karang, metabolit sekunder, antibakteri  Abstract : Sponges are part of the biota that make up the coral reef ecosystem that contains bioactive ingredients. One type of sponge that has a bioactive content is C. aerizusa. The purpose of this study was to see differences in bioactive content in different coral reef conditions. The method used in the coral reef condition survey and the determination of observation stations using the Point Intercept Transect (PIT) method. Examination of secondary metabolites using the thin layer chromatography method according to Harborne (1987), differences in the content of compounds using LC-MS / MS analysis, testing the antibacterial activity using the well method. The results of the research on coral cover conditions showed that the conditions of live coral cover were 19-65% with a bad to good category. The number of compounds at station I reached 15 and the number of compounds in station II reached 13. Secondary Metabolite content for C. aerizusa from both stations was equally active against alkoloid, steroid, flavonoids, terpenoids, and saponins. Antibacterial Potential of n-hexane Extract of C. aerizusa Sponge for stations I and II were not active against E. coli but sponge C. aerizusa station I and II were active against S. mutans activity.Keywords : n-hexane, coral reefs, secondary metabolites, antibacterial
Co-Authors ,, Suryani A.A. Ketut Agung Cahyawan W Abdi Nipangeran Abdul Fajar La Ode Abdul Karim Abdul Khoir HS Abdulkadir Kamaluddin Achmad, SA Ade Dedi Rohayana Adriatman Rasak ADRYAN FRISTIOHADY Adryan Fristiohadi Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiohady Adryan Fristiyohadi Agung Wibawa Mahatva Yodha Agustina Agustina Ahmad Nursodiq Ahmad Zaeni Ajeng Diantini, Ajeng Alyza Zaulya Amalia Alfitrianingrum Amalia, Riezki Amaliah Amaliah Amaliah, Wa Ode Fitri Amir Tajrid Ananda, M Aflahmuna Anas Subarnas Andi Septiana Andini Sundowo Andini Sundowo Andini Sundowo Andini Sundowo Andini Sundowo Andriani, Rina Andryani Ningsih Angriani Fusvita Annisa Fatinah Gani Arba, Muh. Arba, Muhammad Ardiansyah Ardiansyah ARDIANSYAH ARDIANSYAH Arfan Arfan Arfan Arfan Arfan ARFAN ARFAN Ari Sartinah Ari Sartinah, Ari Aries Saifudin Arif Rahman Hakim Asasutjarit, Rathapon Asman Sadino Asman Sadino Asman Sadino ASMAN SADINO, ASMAN Asmawati Asmawati ASNANI ASNANI Asniar Pascayantri Asniar Pascayantri Asnita Hafsani Aspadiah, Vica Asriullah Jabbar Asriullah Jabbar Asriullah Jabbar Asriullah Jabbar Asriullah Jabbar Asrul Sani Asrul Sani, Asrul Asrun Budiatma Astri Alawia Astrid Indalifiany Atasya, Fretiniar Ayu Wulandari Badia, Esti Bafadal, Mentarry Baru Sadarun Baru Sadarun Baru Sadarun Baru Sadarun Baso, La Bonni Rubak Budikafa, Muhammad Jefriyanto Cicilia, Retno Daud, Nur Saadah Dayatriana Muthalib Dewi Puspita Dewiyanti Madu Dian Munasari Diasty Nuraisya Din, LB Dzaky Aulia Rahman EH Hakim Elfahmi Elfahmi, Elfahmi Euis H. Hakim Euis Holisotan Hakim Euis Holisotan Hakim Euis Julaeha Fadeli Ehsan Hanafi Fadhliyah Malik Fahria Nadiryati Sadimantara Fahria Nadiryati Sadimantara Fahriah Nadiryati Sadimantara Faisal Faisal Fajri Fitra Amalia FANNY RAHMADHANI .N Fathan Ramadhan Fatima, Nur Fauzia Azzahra, Fauzia Fauziah, Yulianti Febrianti, Sari Firasmi Sangadji Firdayanti Firdayanti Firdayanti, Firdayanti Firman Oktivendra Fitrawan, La Ode Muhammad Fitrawan, La Ode Muhammad Fredy Talebong Frely Cristiadzi Fawaz Fristiohady Adryan Fristiohady, Adryan Galih, Olga Grasiana Eka E.Y.G Gunawan, Syahrul Gusti Ray Sadimantara Gusti Ray Sadimantara Gusti Ray Sadimantara Hadad, Nur Diana Haera, Evi Hadrawati Hair, La Hajah Ningsih Inta Hakim, EH Hakini, EuisH Halik, Halik Hamsah, Hamsah Handayani, Lili Sri HANIFAH, SYIFA Hariana Haristan, Haristan Harnita, Wa Ode Hartina Hartina Haruna, Lidya A. Haruna, Lidya Agriningsih HASLIANTI HASLIANTI Hasnawati Hasnawati Hasnawati Hasriani H Japar Hastiar, Arfiani Helmia, Wa Ode Nia Hendra Febriansyah Hendrawan Tulungi, Wahyu Hikmayani I W. Sutapa Ibnu Nurrahman, Pandu Idhul Ade Rikit Fitra Idrus, Loly Subhiaty Ilhan Fauzan Ilma Sarimustaqiyma Rianse Ilyas Y, Muhammad Ilyas Y., Muhammad Imam Yahya Imran Imran Imran Indalifiany, Astrid Intan Permata Putri Iren Meylani Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irnawati Irvan Anwar Izzati, Emy J Latip Jabbar, Asriullah Jalifah Latip Jalifah Latip Jamili Jamili Japar, Hasriani H. Juliawaty, LD Juliawaty, Lia D Jumadil Jumriani Jumriani Junli Hong Kadidae La Ode Kamaluddin Kamaluddin Kangkuso Analuddin Kangkuso Analuddin, Kangkuso Kardin, Laode Karimu, Laila Qodriyah Karmilah, Karmilah Khansa Rafida Triaqilah Kholilah, Aulia Kirana, Rezky Marwah Kuncoro, Shienny L. O. A. N. Ramadhan La Ode Ahmad La Ode MJ Purnama La Ode Muh. Fitrawan La Ode Muh. Julian Purnama La Ode Muhammad Andi Zulbayu La Ode Muhammad Julian Purnama La Ode Muhammad Julian Purnama La Ode Muhammad Julian Purnama La OMJ. Permana Laily Bin Din Laily Bin Din Laode Muhamad Mbuyang Nadia Latip, J LB Din LD Juliawaty Lia D. Juliawaty Lia Dewi Juliawaty Lidya Agriningsih Haruna Lidya Agriningsih Haruna Loly Subhiaty Idrus Loly Subhiaty Idrus Lubis, Adryan Fristiohady M. Hajrul Malaka Malaka, Muh. Hajrul Malaka, Muhammad Hajrul Malaka, Muhammad Hajrul Manggau Marianti Mangurana, Wa Ode Intiyani Mardikasari, Sandra Aulia Marianti A Manggau Marianti A. Manggau Marianti, Manggau Martiani Martiani Maryani Masrika, Nur Upik En Maulana Anwar Mawaddah, Nur Ainul Mega Fatimah Rosana Mentarri Bafadal Mesi Leorita Mesi Leorita Mesi Leorita, Mesi Mini Bekti Ningsih Mirawanti G, Wa Ode Mirda Mirda Mirdayani, Eli Mochammad Imron Awalludin Mohammad Ghozali Muamar Abdillah Mubarak Mubarak Mugiarno, Dita Oktavianti Muh. Arba Muh. Ilyas Yusuf Muhamad Handoyo Sahumena Muhamad Handoyo Sahumena Muhammad Alim Marhadi Muhammad Arba Muhammad Arba Muhammad Azhar Muhammad H Malaka MUHAMMAD HAJRUL MALAKA Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Hajrul Malaka Muhammad Ilyas Muhammad Ilyas Y Muhammad Ilyas Y Muhammad Ilyas Y Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Ilyas Yusuf Muhammad Israwan Azis Muhammad Isrul Muhammad Ramadhan Muhammad Ramli Muhammad Rifky Wahyu Alfatih Muhammad Yasir Haya La Ode Muhammad, Taher Muktiarni, Muktiarni Mursanti, Wa Ode Indah Arum Musadar Mappasomba Musdalifa Musdalifa Musdalipah MUSDALIPAH MUSDALIPAH Mustakim Muzuni, Muzuni N. Nohong Nadaoka Kazuo Nadhifatul Aslikah Nasir Nasir Nasir NASRUDIN Nasrudin Nasrudin Nasrudin Nasution, Ali Napiah Nazira, Arni Neneng Andriani Joko Ni Kadek Ayu Berlian Nirwati Rusli Nofriani, Andi Nohong Nohong Nohong, Nohong Nordin Hj Lajis Nubzatussaniyyah, Nunu Nugroho, Ariel Dwiyanto Nunuk Hariani Soekamto Nur Aisyah Abdullah Nur Fadhillah Sahari Sahari Nur Illiyyin Akib Nur Indri Rahayu Nur Salma Nur Syifa Rahmatika Nur Syifa Rahmatika Nur Syifa Rahmatika Nur Syifa Rahmatika Nurcahyani, Fitria Nurhikma Nurhikma Nurhikma Nurkhalis Bintang Astiasal Nurpajriani, Nurpajriani Nurramadhani A. Sida Nurull Hikmah Ode, Muhammad Fajrin Parawansah Parawansah Parawansah Parrung, Arsyani Pascayantri, Asniar Paulina Taba Pauzan Haryono Poko, Feny Riskiana Poko, Feny Rizkia Purnama, La O. M. J. Purnama, La Ode Muh. Julian Purnama, La Ode Muhammad Julian Pusmarani, Jastria Puspita Sari Putri, Natalia Christiani Putri, Olivia Dicha Raden Arfan Rifqiawan Raden Maya Febrianti Rahangga, Dewa Gede Oka Rahman, Sudarman RAHMAT MULIADI, RAHMAT Rahmatika, Nur S. Ramadhan, LOAN Ramadhani, Rizky Barkah Rathapon Asasutjarit Rathapon Asasutjarit Reymon, Reymon RH. Fitri Faradilla Riezki Amalia Rilla Dwi Anjasari Mangidi Rina Andriani Rina Andriani Rina Andriani Rina Andryani Rina Maryanti RINI HAMSIDI Rini Hamsid Rini Hamsidi Roihan Ramadhan, Muhammad Romadhon Haris Alfian Ruslan Ruslin Ruslin Ruslin Ruslin Ruslin Ruslin, Ruslin Rustam HN, Muhammad S. J. Suprapto SA Achmad Saban Rahim Sabarwati, Hadijah Sadimantara, Gusti Ray SADIMANTARA, I GUSTI RAY Saharuddin Sahputra, Adi Salsabila, Syefira Saputri, Ulan Mulyani Saranani, Muhammad Aqsal Azriel Fajar Saripuddin Saripuddin Saripuddin Saripuddin Saripuddin Sariyana, S Sasmita, Wa Ode Indri Selfyana Austin Tee Septiana Andi Sernita Setiawan, La Ode Roni Setiawan, La Ode Wawan Setiawan, Muhammad Azdar Sewan Theeramunkong Shiddiq, Asep Mohamad I Siti Helmyati Siti Rahmawati Ondjo Siti Suriyani Kapa Siti Syafika Sitti Mardiana Sitti Rahmawati Sitti Raodah Nurul Jannah Sitti Raodah Nurul Jannah Sjamsul A. Achmad Sjamsul Arifin Achmad Sjamsul Arifin Achmad Sofa Fajriah Sofa Fajriah Sofa Fajriah Solo, Dian Munasari Sri Hutami Lety Grashella Srimuliana Suaib, S Sulsiah Sulsiah Sunandar Ihsan Supangat Supangat Suryani Suryani Syah, YM Syahrir La Syarif, Aiman Syarul Nataqain Baharum Syarul Nataqain Baharum Syukur, Novita Sari Taher Muhammad Tanzil, Dwi Lestari Khaulani Tatik Handayani, Tatik Thamrin Azis Tian Amalia Halik Tien Tien Titin Syafiransyah Trinovitasari, Nita Trisnaputri, Dian Rahmaniar Ubaidillah W Wahyuni, W W. O. N. Haryanti W. Wahyuni WA KUASA Wa Ode Erna Fitriani Wa Ode Ida Fitriah Wa Ode Jumiati Nur Wa Ode Mutiara Wa Ode Norma Wa Ode Nurfinti Wa Ode Nurfinti Wa Ode Salma Wa Ode Siti Zubaydah Wa Ode Sitti Zubaydah Wa OIL. Kalimin WAHYUNI Wahyuni Wahyuni wahyuni wahyuni Wirasmanto, Bayu Wirhamsah Al-Ramadan Wiwik Rusdin wiwin Wiwin Y, Muhammad Ilyas Yamin Yamin Yana M Syah Yana M. Syah Yana Maolana Syah Yayan Sopyan Yerin Tri Agustina YM Syah Yuliansyah Yuliansyah Yulianti, Sri Samrina Yuliastri, Wa Ode Yunarzat, Ersyanda Yuni Elsa Hadisaputri Yusnaini Yusnaini Zubaydah, Wa Ode Sitti Zulbayu, La Ode Muhammad Andi