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Siti Maimunah
Universitas Muhammadiyah Malang
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Journal : Jurnal Pembelajaran dan Biologi Nukleus

 1 
Aktivitas Antibakteri Ekstrak Daun Jeruk Purut (Citrus hystrix DC) Terhadap Bakteri Staphylococcus aureus || Antibacterial Activity Extract of Leaves of Kaffir Lime (Citrus hystrix DC) Againts of Staphylococcus aureus Bacteria Maimunah, Siti; Rayhana, Rayhana; Silalahi, Yosy Cinthya Eriwaty
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 2: Jurnal Pembelajaran Dan Biologi Nukleus September 2020
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i2.1767

Abstract

Jeruk purut (Citrus hystrix DC) adalah jenis tanaman dari suku Rutaceae yang digunakan sebagai antibakteri. Tanaman merupakan tumbuhan asli Indonesia, yang mengandung flavonoid, tanin, getah, alkaloid, dan minyak esensial. Tujuan penelitian ini untuk menentukan kemampuan ekstrak daun Jeruk purut (C. hystrix DC) sebagai antibakteri terhadap Staphylococcus aureus dan untuk mengetahui konsentrasi yang paling efektif dalam menghambat bakteri Staphylococcus. Penelitian menggunakan daun jeruk purut yang dikeringkan dengan oven pada suhu 45˚C, pulverizing oleh ukuran 40, dan ekstraksi selama 5 hari dengan maserasi ethanol 96%. Penelitian ini menggunakan desain rancangan acak lengkap (RAL) dengan variasi konsentrasi 5%, 10%, 15%, dan 20%, kemudian digunakan untuk menguji aktivitas antibakteri dengan perbedaan disc diffiusion. Zona hambat terbentuk dari ekstrak dengan konsentrasi 5% (6,7 mm), 10% (6,8 mm), 15% (7,3 mm), dan 20% (8,3mm). Hasil analisis data menunjukkan bahwa daun jeruk purut mempengaruhi pertumbuhan Staphylococcus aureus, dimana berdasarkan penelitian nilai sig <0,05 memperoleh nilai dari sig. 0,000, dan konsentrasi 20% merupakan yang paling efektif dalam menghambat pertumbuhan dari S. aureus sebesar 8,3 mm. Ekstrak daun jeruk purut memiliki aktivitas antibakteri. Ekstrak daun jeruk purut dengan konsentrasi 20% merupakan konsentrasi efektif untuk menghambat bakteri.Kaffir lime (Citrus hystrix DC) is citrus plant of Rutaceae's family that was used as an antibacterial. This plant is native to Indonesia, which contains flavonoids, tannins, saponins, alkaloids, and essential oil. This study aimed to determine the ability of antibacterial extract lime leaves against S. aureus and to find out the concentration that is most effective in inhibiting bacteria S. aureus. The research used lime leaves. By drying the leaves at the oven at temperature 45˚C, pulverizing by mesh 40, and extraction by maceration for 5 days with ethanol 96%. This study used a completely randomized design with treatments in various concentrations of 5%, 10%, 15%, and 20% concentration was then used to test the antibacterial activity with disc diffusion. Inhibition zone formed from extracts with concentration of  5% (6,7 mm) 10% (6,8 mm) 15% (7,3 mm) and 20% (8,3 mm). The results of the data analysis showed that the administration of lime leaves extract affected the growth of S. aureus, where the value of sig <0,05 obtained a value of sig 0,000. Based on the research, among the others, the concentration of 20% was that the most effective in inhibiting the growth of S. aureus that was 8,3 mm. Kaffir lime (Citrus hystrix DC.) leaf extract has antibacterial activity against S. aureus. Concentration extract 20% is an effective concentration to inhibit bacterial
Formulation of Red Beet (Beta vulgaris. L) and Aloe Vera (Aloe vera) Gel Extracts as Anti-Aging Lestari, Mutiara Dwi; Maimunah, Siti; Prayoga, Andre
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4478

Abstract

Anti-Aging can prevent or slow down the effects of skin aging. This study aims to formulate extracts of beetroot (Beta vulgaris. L) and Aloe Vera (Aloe Vera) in gel preparations and test their anti-aging effectiveness. This research was conducted at the Laboratory of Pharmaceutical Chemistry, University of Sari Mutiara Indonesia. The tools used are skin analyzer and moisture checker. B. vulgaris Samples from Brastagi, Karo Regency and A.vera taken from Rantauprapat, Labuhan Batu Regency. Methods of implementation Beet extraction was extracted with 96% ethanol solvent, and A.vera extract from the juicer. The irritation test on 12 volunteers used nettle extract cream with the highest concentration of 0.5%. Anti-aging activity testing used 12 female volunteers aged 19-22 years, who were divided into 4 groups, namely 3 volunteers (A; Blank), administration of beetroot extract gel (B. vulgaris. L) and A.vera (B) : 3%), (C: 6%), and (D: 9%). 3. Parameters measured include moisture content, number of pores and wrinkles. Data distribution was analyzed using the One sample Kolmonogrov-Smirnov Test method. Then proceed with the analysis of the One-Way Anova method. The results of research on beet (B. vulgaris. L) and Aloe Vera (A.vera) gel preparations can be formulated in a gel dosage form. pH 6.9, W/O emulsion type, viscosity 2500-3500 cps, non-irritating, and stable on storage for 28 days. Beet fruit extract (B. vulgaris) and aloe vera viscous extract (A.vera) can be formulated in the form of an anti-aging gel that can provide an anti-aging effect at the best concentration, namely at a concentration of 9% by increasing skin moisture to 40.24%; smaller pores 0.53%; and reduce wrinkles by 0.33%
Utilization of Controlled Oven Drying Method to Produce Export Quality of Coffee Peel and Beans (Coffea arabica L.) Maimunah, Siti; Prayoga, Andre; Aruan, Dyna Grace Romatua; Purba, Roberto Roy
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i3.6327

Abstract

Karo regency as supplier of coffee, especially around Jaranguda village. Coffee is one of Indonesia's leading export commodities. A unique approach is required to enhance both the quantity and quality of coffee. Drying is a crucial step to ensure the peel and coffee beans maintain their quality after harvest. This study aims to determine whether drying using a drying oven has an effect on water content and nutritional information. Post-harvest processing methods included wet sorting, separation of peel and coffee beans, washing, drying, analysis of coffee peel characteristics, and analysis of nutrients in coffee beans. The analysis revealed that the water content of Arabica coffee beans was 1.11 %, while the analysis of nutrients and non-nutrients per 100 g of coffee beans revealed the following: total energy 417.34 kcal, fat energy 72.90 kcal, ash content 4.68 %, carbohydrates 72.17 %, total fat content 8.10 %, protein content 13.94 %, saturated fat 3.70 %, and sodium 6.53 mg. The analysis of coffee peel Simplicia revealed water content of 6.69 %, water-soluble extract content of 25.57 %, ethanol-soluble extract content of 13.79 %, ash content of 1.90 %, and acid-insoluble ash content of 1.43%. Analysis results of the characteristics of coffee peel meet the requirements of the Indonesian Herbal Pharmacopoeia Edition II in 2017. The food label includes nutritional value information, also known as nutrition facts, which details the nutritional content of the food ingredients. This information is crucial as it provides valuable insights and enhances the overall value of the product
Uji Aktivitas Antibakteri Ekstrak Daun Sintrong (Crassocephalum crepidiodies) Terhadap Bakteri Staphylococcus aureus || Antibacterial Activity Assay From Sintrong Leaf (Crassocephalum crepidiodies) Against Staphylococcus aureus Bacteria Maimunah, Siti; Pratama, Harji Anggia; Mayasari, Ulfayani
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 1: Jurnal Pembelajaran Dan Biologi Nukleus Maret 2020
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i1.1607

Abstract

Sintrong (Crassocephalum crepidioides) adalah sejenis tumbuhan suku Asteraceae. Kegunaannya sebagai bahan obat tradisional seperti mengatasi gangguan perut, sakit kepala, dan luka bekas bisul biasa dilakukan oleh masyarakat Medan. Penelitian ini bertujuan untuk mengetahui spesifikasi tentang karakteristik, golongan senyawa kimia dan aktivitas antibakteri ekstrak etanol daun Sintrong (C. crepidioides) terhadap bakteri Staphylococcus aureus. Sampel yang digunakan adalah daun sintrong (C. crepidioides) yang diambil dari desa Mupakat Jadi, Kecamatan Bukit, Kabupaten Bener Meriah. Metode penelitian ini dilakukan secara purposif dengan tahapan penelitian yaitu pengambilan bahan tumbuhan, identifikasi, pembuatan simplisia, karakterisasi simplisia, skrining fitokimia, pembuatan ekstrak dan pengujian aktivitas antibakteri. Pengujian aktivitas antibakteri dengan metode difusi menggunakan kertas cakram. Parameter yang diamati yaitu diameter zona hambat pertumbuhan bakteri Staphylococcus aureus di area kertas cakram. Hasil uji aktivitas antibakteri memperlihatkan tingkat efektivitas sedang, dengan konsentrasi 2,5 mg/ml memberikan diameter daerah hambat (9,5 mm) terhadap bakteri Staphylococcus aureus, konsentrasi 5 mg/ml memberikan diameter daerah hambat (10,7 mm) terhadap Staphylococcus aureus, konsentrasi 7,5 mg/ml memberikan diameter daerah hambat (10,9 mm) terhadap S. aureus, dan konsentrasi 10 mg/ml memberikan diameter daerah hambat (11,5 mm) terhadap S. aureus.Sintrong (Crassocephalum crepidioides) is Asteraceae's family plant. In Medan in addition used as a vegetable, sintrong leaves also be used as traditional medicine; among them to cope with stomach disorders, headache, and ulcer scars. The purpose of this research was spesification about characteristic, determination of chemical compounds group and antibacterial activity of ethanol extract, n-heksana fraction, ethylacetate fraction and water fraction of sintrong leaves against Staphylococcus aureus. The sample used was sintrong (Crassocephalum crepidioides) taken from Mupakat Jadi Village, Bukit Subdistrict, Bener Meriah districts. This research purposive stages, namely materials plant, identification, preparation and characterization simplisia, determination the class of chemical compounds of simplex, preparation extract and antibacterial activity tested. Test of antibacterial activity by agar diffusion method used paper disc. Parameters measured were inhibition zone of Staphylococcus aureus. Antibacterial activity test results shows are effectivity, with a concentration of 2,5 mg/ml provides inhibition area diameter (9,5 mm) against Staphylococcus aureus bacteria, a concentration of 5 mg/ml provides inhibition area diameter (10,7 mm) to Staphylococcus aureus, a concentration of 7,5 mg/ml provides inhibition area diameter (10,9 mm) to Staphylococcus aureus, and a concentration 10 mg/ml provides inhibition area diameter (11,5 mm) to Staphylococcus aureus.
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