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All Journal Jurnal Hukum Khaira Ummah Jurnal Civics: Media Kajian Kewarganegaraan Jurnal Pendidikan Geografi Undiksha Sains Olahraga : Jurnal Ilmiah Ilmu Keolahragaan Tunas Geografi Animal Agricultural Journal Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Biogenesis: Jurnal Ilmiah Biologi Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Jurnal Ternak Tropika Majalah Obat Tradisional EL-IDARE: JURNAL MANAJEMEN PENDIDIKAN ISLAM BANGUN REKAPRIMA JPG (Jurnal Pendidikan Geografi) Monex: Journal of Accounting Research HIGEIA (Journal of Public Health Research and Development) Journal of Health Education JSSF : Journal of Sport Science and Fitness The Journal of Educational Development JURNAL KEPEMIMPINAN DAN PENGURUSAN SEKOLAH Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal Visipena Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Jurnal Perikanan Universitas Gadjah Mada Biosaintifika: Journal of Biology & Biology Education JURNAL ILMIAH PETERNAKAN TERPADU Edukasi Islami: Jurnal Pendidikan Islam Al-Madrasah: Jurnal Ilmiah Pendidikan Madrasah Ibtidaiyah Anterior Jurnal Jurnal Medik Veteriner Fair Value: Jurnal Ilmiah Akuntansi dan Keuangan Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) Jurnal Basicedu Jurnal SPORTIF : Jurnal Penelitian Pembelajaran EQIEN - JURNAL EKONOMI DAN BISNIS JOURNAL OF SCIENCE AND SOCIAL RESEARCH Indonesian Journal for Health Sciences (IJHS) KOMPUTIKA - Jurnal Sistem Komputer PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Bimbingan Konseling Journal of Physical Education and Sports Jurnal Gentala Pendidikan Dasar Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Social, Humanities, and Educational Studies (SHEs): Conference Series Jurnal Pengembangan Penyuluhan Pertanian EDUTEC : Journal of Education And Technology GEOGRAPHY : Jurnal Kajian, Penelitian dan Pengembangan Pendidikan JOSSAE (Journal of Sport Science and Education) Jurnal Abdi Insani Jurnal Maenpo : Jurnal Pendidikan Jasmani Kesehatan dan Rekreasi jurnal ilmu keolahragaan Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Dehasen Educational Review Indonesian Journal of Global Health research BioEksakta : Jurnal Ilmiah Biologi Unsoed Bantara Journal of Animal Science Asimetris: Pendidikan Matematika dan Sains Jurnal Pendidikan dan Teknologi Indonesia Jurnal Pendidikan Narra J Journal of Digital Learning and Education Jurnal Ekonomi AbdiTechno (Jurnal Pengabdian Masyarakat) Jurnal Pengabdian kepada Masyarakat Bina Darma International Journal of Economic, Business, Accounting, Agriculture Management and Sharia Administration (IJEBAS) El-Jughrafiyah : Jurnal Geografi dan Terapannya Journal of Education Research Educenter: Jurnal Ilmiah Pendidikan Jurnal Akuntansi, Ekonomi dan Manajemen Bisnis Jurnal Manajemen Dakwah International Journal of Social Science, Education, Communication and Economics Journal of Lex Philosophy (JLP) Jurnal Basicedu Prosiding University Research Colloquium Yuris: Journal of Court and Justice Acitya Wisesa: Journal of Multidisciplinary Research Journal of Public Health Concerns Jurnal Pengabdian Kolaborasi dan Inovasi IPTEKS Sewagati Indonesian Journal of Community Empowerment (IJCE) SOROT: Jurnal Pengabdian Kepada Masyarakat Batik-Mu : Jurnal Pengabdian Masyarakat Journal of Animal Research and Applied Science Riwayat: Educational Journal of History and Humanities Jurnal Akuntansi, Keuangan, Perpajakan dan Tata Kelola Perusahaan Majority Science Journal EDUCENTER JURNAL PENDIDIKAN Journal of Humanities Education Management Accounting and Transportation Jurnal Agripet Animal Production Holistik Jurnal Kesehatan Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) BACA: Jurnal Dokumentasi dan Informasi Jurnal Keselamatan Transportasi Jalan Journal of Strength and Conditioning TABELLIUS Ascendia: Journal of Economic and Business Advancement Folia Medica Indonesiana
S. Sugiharto
Department Of Animal Science, Faculty Of Animal And Agricultural Sciences, Diponegoro University
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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IMPROVING THE COMPETENCY OF MOLECULAR GENETICS FOR MGMP BIOLOGY TEACHERS IN SENIOR HIGH SCHOOL IN SURABAYA Sugiharto, Sugiharto; Hayati, Alfiah; Amin, M. Hilman F.
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.010-018

Abstract

Molecular Genetics is a compulsory subject in grade 12 of senior high school, covering discussions on Genetic Substances (DNA and RNA), Heredity, Protein Synthesis, Mutations and Biotechnology. Many seniors high school students have difficulty understanding this course, so their report book scores are below the KKM. One of the problems is no practicum to apply this subject because it is constrained by tools, materials, and the skills of the teaching staff at each school. Teachers are a very strategic intermediate target because they will explain directly to their students. The purpose of this community service activity is to improve the competence of teachers who are members of the MGMP Biology in Surabaya, especially in the molecular field. This activity was carried out on Saturday, June 30, 2024, attended by 24 participants coordinated by the Head of the MGMP Biology Surabaya, located at the Molecular Genetics Laboratory, Department of Biology, Faculty of Science and Technology, Airlangga University. During the training, participants were given courses and practicums such as the use of micropipettes, the DNA isolation process, the PCR method and the DNA electrophoresis process. The results of the training showed an increase in teachers' understanding as seen from the comparison of achievement scores between the pre-test (highest score = 50, range of score ​​10 – 20 = 45.83%) and post-test (highest score = 80, range of score ​​10 – 20 = 29.17%); the enthusiasm of participants during the delivery of the courses, practicums and discussions; and the satisfaction index value (above 95%) given by participants. It can be concluded that the Molecular Genetics technique training activities using the PCR and Electrophoresis methods can improve the competence and skills of MGMP Biology Surabaya teachers as an effort to support the learning process in senior high schools. From these results, it is hoped that teachers can be clearer when explaining Molecular Genetics material to their students so that students can understand the material more easily.
Development of Interactive Learning Media with Wordwall to Enhance Student Interest and Understanding in Class X Geography Harahap, Robby Risky; Rahmadi, Muhammad Taufik; Sugiharto, Sugiharto; Damanik, Muhammad Ridha Syafii
JPG (Jurnal Pendidikan Geografi) Vol 12, No 1 (2025)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpg.v12i1.21022

Abstract

This research aims to develop wordwall-based learning media and determine students' learning interests and determine students' understanding of remote sensing material. The research subjects were 33 class X 3 students. The research method used is Research and Development (R&D) with reference to the simplified Borg & Gall steps. The research instrument was validated through material experts and media experts to ensure content validity. The research results show that Wordwall-based learning media makes a positive contribution to students' interest in learning. This is shown by an increase in students' comprehension scores after using the media. Apart from that, students' interest in learning about remote sensing material is in the good category, namely high interest. The research results show that Wordwall-based learning media is suitable for use as an alternative innovation in the geography learning process. The conclusion of this research is that Wordwall-based learning media is effective in increasing students' interest and understanding of remote sensing material. This media can be a reference for teachers to create more interactive and interesting learning. This research also recommends further development to integrate other digital learning media in the teaching and learning process.
Performance and digestive organ profile of Wandering Whistling Duck fed different protein levels Jamilah, J.; Mahfudz, L. D.; Suprijatna, E.; Wahyuni, H. I.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 2 (2025): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.2.92-102

Abstract

This study investigated the effects of different dietary protein levels on the performance and diges-tive tract profile of Wandering Whistling Ducks. A total of 108 5-month-old Wandering Whistling Ducks were reared intensively for three months (one-month rearing and feed adaptation period fol-lowed by two months of dietary intervention). The dietary treatments comprised of three protein levels: T1 12%, T2 15%, and T3 18%. Each treatment consisted of nine replications, with each experimental unit consisted of four Wandering Whistling Ducks. The parameters measured in this study were feed consumption, water consumption, feed-to-water ratio, body weight gain, relative weight and length of digestive organ, villus height, crypt depth, villus-to-crypt ratio, histopathological features, digestive tract pH, and ileal bacteria counts. The data were analyzed using analysis of variance followed by Dun-can’s multiple range test. The results indicated that dietary protein levels significantly affected feed consumption, body weight gain, ileal crypt depth, cecal lactic acid bacteria, and gizzard pH. Other pa-rameters showed no significant response to different protein levels. Based on these findings, it was concluded that a dietary protein level of 18% yielded the most favourable outcomes in terms of growth performance and the digestive tract profile of Wandering Whistling Ducks.
Improving broiler growth and immunity with encapsulated Cosmos caudatus and Andrographis paniculata extracts at high stocking density Agusetyaningsih, I.; Krismiyanto, L.; Yudiarti, T.; Widiastuti, E.; Sari, D. A. P.; Pawesti, S. D.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 2 (2025): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.2.111-121

Abstract

The community's growing demand for chicken meat as an animal protein source has presented farmers with the challenge to alter the care of broiler chickens by using high cage density. The aim of this study is to investigate the effect of diet supplementation with encapsulated extract of kenikir (Cosmos caudatus) and bitter (Andrographis paniculata) extracts on broiler diet to growth perfor-mance, immunity status, and carcass proportion. A total of 370 day-old Cobb broiler chicks, weighing 45.25 ± 0.89 g, were divided into five groups: T0: control, basal diet + density 10 chicks/m2, T1: basal diet + density 16 chicks/m2, T2: basal diet + density 16 chicks/m2 + Cosmos caudatus 1 g/kg feed, T3: basal feed + density 16 chicks/m2 + Andrographis paniculata 1 g/kg feed, T4: basal feed + density 16 chicks/m2 + Cosmos caudatus 0.5 g/kg feed + Andrographis paniculata 0.5 g/kg feed). At the end of the study, the blood sample and internal organ were collected to further analysis. The final weights of groups T2, T3, and T4 were better than T0 and T1, while T1 has the lowest weight among the other groups. Chicks in T0, T2, T3 and T4 had lower PDW levels compared to T1. Chicks in T4 have a high-er jejunum villi height compared to T0, T1, T2 and T3 groups. The treatments did not affect the small intestine and giblets relative organ wights (P>0.05). The group of T0, T2, T3, and T4 have a fewer duo-denum lesion compared to T1. The group of T2, T3, and T4 have a better structure in jejunum cells compared to T0 and T1. Lesion score of Bursa Fabricius and spleen were greater in T3 and T4 com-pared to T0, T1 and T2 groups. And T1 have the lowest ND antibody compared to other groups. In con-clusion, giving encapsulation of kenikir (Cosmos caudatus) and bitter (Andrographis paniculata) ex-tracts on broiler diet can improve production performance and immune status of broiler chickens.
Produksi Cabai Melalui Teknik Hidroponik Irigasi Tetes untuk Meningkatkan Pendapatan Komunitas Pemuda Tentena, Kabupaten Poso, Sulawesi Tengah Manuhara, Yosephine Sri Wulan; Wibowo, Anjar Tri; Sugiharto, Sugiharto; Sugiarso, Djarot
Sewagati Vol 9 No 3 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i3.2322

Abstract

Kota Tentena adalah salah satu pusat wisata dengan adanya danau Poso yang sangat terkenal. Namun masih terdapat  masyarakat yang hidup miskin. Kelompok pemuda Pukat Tentena di desa Tentena, Kabupaten Poso merupakan kelompok yang mengarah pada ekonomi produktif yang dalam beberapa tahun ini memiliki usaha pertanian sayur dan buah secara hidroponik untuk menampung pemuda dari daerah pelosok yang ingin melanjutkan sekolah namun tidak memiliki biaya. Namun masih banyak pemuda yang belum dapat dibantu untuk membiayai sekolah mereka. Sementara itu Komunitas Pemuda masih memiliki lahan yang tidak produktif. Oleh karena itu melalui program Kemitraan Masyarakat ini dilakukan kegiatan untuk memanfaatkan lahan tidak produktif tersebut sebagai lahan produksi cabai melalui teknik hidroponik irigasi tetes. Metode pelaksanaan dilakukan dalam beberapa tahap yaitu pembuatan rumah kaca (green house), pelatihan budidaya cabai menggunakan teknik hidroponik sistem irigasi tetes, penanaman benih cabai, pembuatan hidroponik sistem irigasi tetes, pemindahan dan pemeliharaan bibit cabai dalam sistem irigasi tetes.Setelah dipelihara selama lebih kurang 60 hari tanaman mulai berbunga dan berbuah. Untuk pemanenan cabai bisa dilakukan beberapa kali. Pada panen perdana ini diperoleh lebih kurang 250 g per tanaman. Hasil penjualan cabai dapat menambah pendapatan pemuda Tentena sebagai sasaran Mitra Pengabdian.
Transformasi Pembinaan Olahraga Prestasi Di Sekolah: Implementasi Metode Sport Search Dalam Mengidentifikasi Dan Mengembangkan Bakat Olahraga Peserta Didik Angga, Prayogi Dwina; Sugiharto, Sugiharto; Hariyanto, Eko; Merawati, Desiana; Pranoto, Adi; Kardiyanto, Deddy Whinata
Jurnal Pengabdian kepada Masyarakat Bina Darma Vol. 5 No. 1 (2025): Jurnal Pengabdian kepada Masyarakat Universitas Bina Darma
Publisher : DRPM-UBD

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33557/t8mtp686

Abstract

Tujuan kegiatan pengabdian kepada masyarakat ini adalah mengoptimalkan pembinaan prestasi olahraga pada peserta didik di sekolah melalui pendekatan pemanduan bakat dengan menggunakan metode sport search. kegiatan ini dilaksanakan bekerja sama dengan Kelompok Kerja Guru (KKG) Pendidikan Jasmani, Olahraga dan Kesehatan (PJOK) Kabupaten Malang. Metode bimbingan teknis digunakan dalam kegiatan ini yang meliputi beberapa tahapan, yakni identofikasi masalah dan potensi mitra, pemaparan materi pemanduan bakat dan metode sport search, praktik tes pemanduan bakat, analisis hasil menggunakan aplikasi sport search, serta monitoring dan evaluasi. Kegiatan ini telah memberikan peningkatan pemahaman dan keterampilan guru dalam mengidentifkasi bakat olahraga siswa secara lebih objektif dan ilmiah. Pendekatan metode sport serach yang digunakan dalam pemanduan bakat sangat membantu guru dalam mengenali potensi atau bakat olahraga peserta didik dengan lebih akurat. Evaluasi kepuasan yang disampaikan oleh peserta dalam kegiatan ini juga mengindikasikan bahwa kegiatan ini memberikan kontribusi secara nyata dalam pembinaan olahraga yang berbasis ilmiah di sekolah. Kegiatan ini dapat menjadi solusi inovatif dalam upaya mengoptimalkan potensi peserta didik dan pembinaan prestasi olahraga di sekolah. Keberlanjutan kegiatan ini perlu diperkuat dengan melakukan pendampingan yang berkesinambungan serta melaksanakan kolaborasi dengan berbagai pihak agar implementasinya dapat diperluas di sekolah lainnya.
Sosialisasi Penerapan Materi Pengantar Akuntansi di Politeknik METrO Betong Sarawak Sugiharto, Sugiharto; Primasiwi, Asri; Ibrahim, Rosida; Rahayu, Nika Esti
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 8, No 2 (2025): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v8i2.8256

Abstract

Kegiatan pengabdian masyarakat ini dilaksanakan di Politeknik METrO Betong Sarawak (PMBS) untuk mengatasi masalah kurangnya pemahaman mahasiswa terhadap prinsip-prinsip dasar akuntansi, yang berdampak pada kemampuan mereka dalam mengelola laporan keuangan dan analisis data akuntansi. Pemilihan topik ini penting karena akuntansi merupakan keterampilan esensial yang diperlukan dalam dunia kerja, terutama di sektor keuangan yang terus berkembang. Tujuan dari pengabdian ini adalah untuk meningkatkan pemahaman mahasiswa mengenai konsep dasar akuntansi dan relevansinya dalam kehidupan sehari-hari serta dunia usaha. Metode yang digunakan dalam kegiatan ini meliputi ceramah, diskusi interaktif, dan simulasi kasus. Kegiatan ini melibatkan 47 mahasiswa dari jurusan Kewangan di PMBS, di mana mereka dibagi ke dalam kelompok kecil untuk mendorong diskusi dan analisis kasus. Hasil dari kegiatan sosialisasi menunjukkan peningkatan pemahaman peserta, dengan 63,82% mahasiswa memiliki pemahaman akuntansi yang sangat baik. Kesimpulannya, kegiatan ini tidak hanya berhasil meningkatkan pemahaman akuntansi mahasiswa, tetapi juga memperkuat hubungan kerja sama antara Politeknik Negeri Madiun dan PMBS, yang dapat berlanjut ke program-program pengembangan lainnya di masa mendatang.
Improving Learning Outcomes in Whole Numbers Operations Through Realistic Mathematics Education Assisted by Dienes Blocks Putrinanda, Marcia; Karo, Putri Vanessya P. Br; Mairing, Jackson Pasini; Sugiharto, Sugiharto
JURNAL PENDIDIKAN Vol 26 No 1 (2025): Jurnal Pendidikan
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/jpn.v26i1.19152

Abstract

Penelitian ini dilatarbelakangi oleh rendahnya pemahaman siswa dalam konsep operasi hitung bilangan kecil. Tujuan penelitian ini adalah mendeskripsikan penerapan model Pembelajaran Matematika Realistik berbantuan Blok Dienes untuk meningkatkan hasil belajar siswa kelas IV SDN 1 Paring Lahung. Penelitian ini dilaksanakan selama dua siklus. Jenis penelitian yang digunakan adalah Penelitian Tindakan Kelas (PTK). Teknik pengumpulan data menggunakan observasi, tes, dan dokumentasi. Hasil penelitian menunjukkan bahwa pada siklus I jumlah siswa yang memperoleh nilai ketuntasan minimal 70 sebanyak 1 siswa (11,1%) dan yang tidak memenuhi nilai ketuntasan minimal 70 sebanyak 8 siswa (88,8%) dengan nilai rata-rata siswa sebesar 25, 8. Pada siklus 2 mengalami peningkatan yang signifikan, jumlah siswa yang memenuhi nilai ketuntasan minimal 70 sebanyak 10 siswa (90,9%) dan yang tidak memenuhi nilai ketuntasan minimal 70 sebanyak 1 siswa (9,1%) dengan nilai rata-rata siswa sebesar 84 ,8. Hasil belajar siswa mengalami peningkatan dari siklus I ke siklus II sebesar 79,8%. Dapat disimpulkan bantuan bahwa penerapan model Pembelajaran Matematika Realistik dengan Blok Dienes dapat meningkatkan hasil belajar siswa kelas IV SDN 1 Paring Lahung pada operasi hitung bilangan kecil.
Dynamics of Integrated Social Studies Learning Implementation in Junior High Schools: Case Study at SMPN 41 Medan Wardani, Aulia; Efranita Purba, Desi Marcelina; Batubara, Rina Mawardah; Sugiharto, Sugiharto; Lubis, Darwin Parlaungan; Novira, Nina; Permana, Sendi; Vanda, Vanda
Tunas Geografi Vol. 13 No. 2 (2024): JURNAL TUNAS GEOGRAFI
Publisher : Department of Geography Education, Faculty of Social Sciences, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/tgeo.v13i2.65527

Abstract

Integrated social studies learning is a form of integrative learning applied in junior high schools by integrating several disciplines into one subject, namely geography, economics, sociology, and history. This provides obstacles for teachers teaching integrated social studies learning. The study aimed to determine the teachers' constraints and students' understanding of integrated social studies learning at SMPN 41 Medan. The research method used is a descriptive qualitative method. The study results explain that the teacher's constraints in integrated social studies learning include difficulty understanding learning material outside the science teacher's field, limited time allocation, lack of learning facilities and infrastructure, and differences in each student's characteristics. Student understanding in terms of Integrated IPS learning is very high. This can be seen from students' interest during the learning process. It attracts students' attention with a percentage of 87.5% so that the majority of students in this situation feel the learning process is enjoyable and is considered a positive indication that teaching methods and strategies the learning used by the teacher has succeeded in attracting student interest so that they can understand the Integrated Social Sciences learning material. Students' understanding of social studies learning can be a benchmark for teacher success in integrating several disciplines into one subject.
Plant-based synthesis of gold and silver nanoparticles using Artocarpus heterophyllus aqueous leaf extract and its anticancer activities Dewi, Firli RP.; Rohmatika, Aulia U.; Jamil, Arniza KM.; Demircan, Turan; Idris, Muhammad F.; Litazkiyyah, Litazkiyyah; Fahmi, Muhammad; Rosyidah, A'liyatur; Hayati, Alfiah; Sugiharto, Sugiharto
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1770

Abstract

Green synthesis of nanoparticles has garnered significant attention for its sustainable and environmentally friendly approach. Despite extensive research on Artocarpus heterophyllus-derived nanoparticles using seeds, fruits, and rind, the therapeutic potential of its leaf extract remains largely unexplored, particularly in MCF-7 breast cancer cells. The aim of this study was to investigate the potential of aqueous leaf extract from A. heterophyllus as a reducing and capping agent to synthesize silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs), as well as to evaluate their anticancer efficacy. The nanoparticles were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and particle size analysis to confirm the formation. To evaluate anticancer potential, key oncogenes associated with cancer proliferation and survival were analyzed, including c-Myc, cyclin D1, human epidermal growth factor receptor-2 (HER-2), microRNA-622 (miR-622), and cyclooxygenase-2 (COX-2). The present study demonstrated that AgNPs and AuNPs synthesized from A. heterophyllus extract had distinct sizes and shapes, with AgNPs averaging approximately 12.75 nm and exhibiting a spherical morphology, while AuNPs averaged 109.26 nm and had a pentagonal shape. Furthermore, AuNPs had no anticancer activity. In contrast, AgNPs showed potent anticancer effects, with inhibitory concentration (IC50) values of 124.626 and 54.981 µg/mL at 48 and 72 hours, respectively. The AgNPs treatment increased the proportion of cells in G2/M phase, indicating the induction of mitotic catastrophe leading to cell death. AgNPs downregulated the expression of several oncogenes associated with cancer cell proliferation and survival (cyclin D1, COX-2, HER-2, and miR622), but did not significantly reduce c-Myc expression. In conclusion, AgNPs derived from A. heterophyllus leaf extract have significant potential as a novel therapeutic agent in cancer treatment while preserving its biocompatibility, emphasizing the promise of sustainable and cost-effective synthesis of plant-based nanoparticles.
Co-Authors A. Bachtar, Mulni A. N. M. Ramli A. Pratama A. Pratama A. R. Pratama A. Setiaji A. Setiaji AA Sudharmawan, AA Abbas, Ilham Abda Abda Abdul Chalim, Abdul Abdul Hasan Saragih Abdulloh, Ido Nur Adi Pranoto, Adi Adi Pratama, Okky Rizki Aditaruna, Djadjang Adli, Danung Nur Agusetyaningsih, I. Ahmad Zain Sarnoto Akhrima, Nina Akmal, Yurizal Al Gifari, Habibulah Ahmad Al-Baarri, Ahmad Ni'matullah Alfani, Muhammad Aliffajaruddin Alfiah Hayati Alfinda Novi Kristanti Alfindo, Bernadito Wildan Aloysius Adya Pramudita Amalia, Sa'adatul Amin, M. Hilman F. Amirudin Amirudin Anak Agung Istri Sri Wiadnyani Angga, Prayogi Dwina Anies Setiowati, Anies Anik Setyo Wahyuningsih Anugrah Robby Pratama Anugrah Rossytasari Anugrah, Siti Dian Anwar Anwar Anwar, Mahfud Anwardi, Nandina Salsa Billa Aprianto, Boby Arbiansyah Arbiansyah, Arbiansyah Ardiansah, Wisnu Arief Firmansyah Aristiyanto, Aristiyanto Arrofi’, Abdullah Dzulfikri Arum Pratiwi Atang, Atang Atika Swandana Awalya Awalya, Awalya Awan Hariono Azis, T. Abdillah Aziz Syaifudin Mursyid Aziz, Mohammad Fahthul Azizah, Fatiya Nur Azzarah, Regita Asiah B. Sukamto Bachtiar Efendi, Bachtiar Batubara, Efira Andiyani Batubara, Rina Mawardah Beti Kristinawati Bianca, Tanya C. Lauridsen Cahya Setya Utama Ciptaning Wahyu W, Imam Santosa D. A. Lestari Dalimunthe , Amirhud Dallion, Ryan Dalmeri Dalmeri Damanik, Muhammad Ridha Syafii Darlani, Darlani Darmawan, Kurnia Sobar Darwin P. Lubis, Darwin Demircan, Turan Desiana Desiana, Desiana Desiana Merawati Devanti, Dila Adelia Dewi, Firli Rahma Primula Dewi, Firli RP. Dewi, Rina Dian Farida Asfan Dian Kusumaningrum Dian Purnomo Dian Wahyu Harjanti Diana Zuhro Diana, Ridma Djarot Sugiarso Durubanua, Derita Ramai Dwi Cahyo Kurniawan, Dwi Cahyo E. Kurnianto E. Kusumanti E. Suprijatna, E. E. Widiastuti E. Widiastuti E. Widiastuti E. Widiastuti Efranita Purba, Desi Marcelina Eko Hariyanto Eko Setio Wibowo Endang Widiastuti Eni Yuniastuti Enny Istanti Enung Siti Nurhidayah Eram Tunggul Pawenang Erli Mutiara Erya, S. N. Esra Parmian Talenta Siburian Evi Widowati F. Wahyono Fahira, Rachyana Faizah Betty Rahayuningsih Farid Farid Agushybana Fatah, Bagus Ari Fathil, Nur Ezza Fazleen Mohd Fatimah Azzahra Fauzi, Ekan Fauzia, Alysya Fazera, Della Febriana, A. Fellix Rimba Fikri Sasongko Widyatama Firdaus, Alif Fitri Laila, Fitri Fortino, Edbert Friantini, Rizki Nurhana Fuadhy Wahyu Fahmi Gilbert Nathaniel Ginting, Hans Giovani GS , Achmad Daengs Gumelar, Rixco Gesang Gunawan Arsyad, Eriek Gunawan Gunawan H. I. Wahyuni H. I. Wahyuni H. I. Wahyuni H. I. Wahyuni H. Wahyuni H. Wahyuni H.I. Wahyuni Hadi, Tjokro Hadida, Vanya Shaquila Hadiningrum, Sri Hafidza, Hikmah Halim, Jennifer Halim, Marwa Halleina Rejeki Putri Hartono Hamzah Fansuri Hana Hana, Hana Handari, Miana Ciputra Hanny Indrat Wahyuni Hanny Indrat Wahyuni Harahap, Robby Risky Hari Soepriandono Harjanto, Eko haryani, Desti Haryanto Haryanto Hasna, N. A. Henckel, P Herry Koesyanto Heru Budianto, Bambang Hexa Apriliana Hidayah Hidayat, Dina Damiyanti Hidayat, Mohammad Fatqi Holanda, Stevanny I. Agusetyaningsih I. Agusetyaningsih I. Isroli I. Mangisah I. Mangisah I. Mareta Ibrahim, Rosida Idris, Muhammad F. Ifada Mufidatul Ula Ihsanuddin, Mokhammad Imam Suroso Indri Mareta Irada Sinta isfenti sadalia Ismadi, V. D. Y. B. Ismail Ismail Ivanna, Julia Jackson Pasini Mairing Jackson Pasini Mairing, Jackson Pasini Jamhari, Syafek Jamil, Arniza KM. Jamilah, J. Junaidi Junaidi Jupriadi, Jupriadi K, Abdul Hamid Kamariah Tambunan Kardiyanto, Deddy Whinata Karnain, Bambang Karo, Putri Vanessya P. Br Kartinah , Kartinah Khairani, Siti Khairul Amri Khoirul Hidayat Kirowati, Dewi Kudhori, Ahmad Kusman, Agus Kusuma, Dannis Yuda Kusuma, Donny Wira Yudha L. D. Mahfudz L. D. Mahfudz L. Lauridsen Lauridsen, C Lestari, D. A. Lestariningsih, Tri Lilik Krismiyanto Lim, Jeksen Lisdayana, Nurmalisa Litazkiyyah, Litazkiyyah LUBIS, AMINAH Lukas Lukas Luthfian, F. M. Irfan M. R. Yousaf Mahalul Azam Maharani Ritonga Manalu, Febbry Vebilola Manihuruk, Waldio Manikya Pramudya Mardhatillah, Mardhatillah Martono Martono Mateus Sakundarno Adi, Mateus Sakundarno Maulia, Tria Mbina Pinem Mubasyiroh, Nabila Ainaya Mudaliya, Eva Muhammad Azinar MUHAMMAD FAHMI Muhammad Hilman Fu'adil Amin Muhammad Irfan Sulfani Mu’tamar, M Fuad Fauzul N. S. Pandupuspitasari N.S. Prabowo Nanang Indardi, Nanang Nashruddin, Ahmad Nasrullah Nasrullah Nasuka Nasuka, Nasuka Nasution, Awal Kurnia Putra Nawi, Syahruddin Nazila, Nur Alvisyahri Niken Fatimah Nurhayati, Niken Fatimah Ningrum, Rahayu Setya Novan, Christian Novira, Nina Nowak, Agnieszka Magdalena Nugroho, Ardi Setyo Nugroho, Sasmito Widi Nuning Setyaningrum Nur Azizah Nurbulan, Eka Nurcahyani, Dwi Nurhana Friantini, Rizki Nurhayati, Defi Nurlaila, Lala Nurmuhammad, Riezky Zulfikar Nurwantoro . Oktaviana Bangun Oktia Woro Kasmini Handayani Oktriyani, Oktriyani Olivia Andiana P. Henckel Pambudi, Pria Bagus Pane, Aina Ristanti Parhadi Parhadi, Parhadi Pasaribu, Rosidah Minta Ito Pawesti, S. D. Pinardi, Janu Prastiwanti Nur Jannah Pratama, A. R. Pratama, Alvin Pratama, Anugrah Robby Pratiwi Sembiring, Yolanda Pratiwi, Kristiani Dina Prihadyatama, Ardila Prihatin Lumbanraja Primasiwi, Asri Pristiwanti, Pristiwanti,N. Priyo Susatyo Probowati, Banun Diyah punding, walter Puspitawati Puspitawati Putera, Shidqi Hamdi Pratama Putri, Nandila Maysa Putri, Nindy Asyifa Putrinanda, Marcia R. Halimatunnisroh R. Murwani Rahayu, Nika Esti Rahmadani, Adinda Ayu Rahmadi, M Taufik Rahmadi, M. Taufik Rahmadi, Muhammad Taufik Rahmat Winata Rahmawati, Nur Aini Faridah Rakhmawati Rakhmawati Ramadani, Yunda Fitri Ramdes, Muhammad Raza, M. A. Raza, Muhammad Asif Rejeki, Purwo Sri Rezky, Sri Ricki Ananda Riskiana, Maya Rizki Aprilia Putri Rohani Rohani Rohmatika, Aulia U. Rosyidah, A'liyatur Rosyita, Hafna S. Kismiati S. R. Rini S. Sumarsih S. Sutaryo Sahat Siagian Sahri Sahri Saleh, Munandi Samporno, Kholib Saragi, Emi Juwita Saragih, Rosvilani Sari S, Saurlin Febriana Sari, D. A. P. Sartono, T. A. Sartono, Tri Agus Sembiring, Rafika Sari Br Sembiring, Yolanda Pratiwi Sendi Permana Setiaji, Asep Setiowati, Tutut Setiyono, Eko Setyawan, Dimas Ari Setyono, Karnawan Joko Sholikhah, Anindya Mar'atus Sholikin, Muhamad Misbakhul Shunhaji, Akhmad Sianturi, Roy Sihombing, Evi Ariyanti Marlina Silalahi, Yulianda Ghrace Simagunsong, Tohap Sintong, Mahara Siregar, Iqbal Kamil Siregar, Nova Desima Handayani Siti Mayang Sari Sitinjak, Sella Oktavia Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sukmaningrum Sri Yunita Suciani, Ayu SUDARMONO SUDARMONO Sujiyanti, Sujiyanti Sukmawati, Betty Sulastri Sulastri Suparman Suparman Supriyadi Supriyadi Supriyanto Supriyanto Supriyo Supriyo Suroso Suroso Suryaningsih, Suhestri Susena, Satrio Jaya Sutarno Sutarno Sutopo Sutopo Suyana, Nana Syahbana, Fadil Ali Syarifah Syarifah, Syarifah T. A. Sartono T. A. Sartono T. Ayasan T. Ayaşan T. Sartono T. Sartono T. Yudiarti T. Yudiarti T. Yudiarti Tandiyo Rahayu Tanjung Puspa Maldini Taufiq Hidayah Tea , Rukman Tentrawinata, Z. I. Titi Chasanah Tri Anjar Tripure Maas, Linda Trisna Ayu Oktavia Tupan Tupan Turrini Yudiarti Utami, Fitra Isna V. D. Yunianto Vanda, Vanda Vinami Yulian W. Winarti Wardani, Aulia Wibawa, Koerniawan Dwi Wibowo, Sundaru Guntur Widiastuti, Endang Widodo, Nova Maulud Wijayanti, Ely Tri Wijayanti, Gratiana Ekaningsih Wijinindyah, Ayutha Wildana Latif Mahmudi, Wildana Latif William William Windiarini Lestari Wita Oktaviana Wiwiek Natalya Wulandari, E. C. Yogi Putranto Yosephine Sri Wulan Manuhara Yuliana, Afid Yupi Royani Yupito, Yupito Yusuf, R. Zam, Mahalul