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All Journal International Journal of Public Health Science (IJPHS) JURNAL HUTAN LESTARI Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Jurnal Educhild : Pendidikan dan Sosial Jurnal Kreatif Tadulako Online Elkom: Jurnal Elektronika dan Komputer KONSELI: Jurnal Bimbingan dan Konseling (E-Journal) Indonesian Journal of Urology Jurnal Promosi Pendidikan Ekonomi Indonesian Journal of Tropical and Infectious Disease Jurnal Counsellia Indigenous: Jurnal Ilmiah Psikologi EDUKASI PAUDIA: JURNAL PENELITIAN DALAM BIDANG PENDIDIKAN ANAK USIA DINI Jurnal Ilmiah Ekonomi Islam Jurnal AKP Language Literacy: Journal of Linguistics, Literature, and Language Teaching Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya JURNAL KONSELING GUSJIGANG BIOEDUKASI JPM (Jurnal Pemberdayaan Masyarakat) JURNAL WACANA KESEHATAN Edukasi : Jurnal Penelitian dan Artikel Pendidikan Journal of Honai Math Pendas : Jurnah Ilmiah Pendidikan Dasar JTAM (Jurnal Teori dan Aplikasi Matematika) JURNAL EKSAKTA PENDIDIKAN (JEP) Jurnal Pendidikan Kebutuhan Khusus Jurnal SEGAR Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL ILMIAH CAHAYA PAUD JURNAL PENDIDIKAN TAMBUSAI Jurnal Basicedu Jurnal Golden Age Jurnal Ilmiah Keperawatan Sai Betik Islamic Guidance and Counseling Journal JURKAM: Jurnal Konseling Andi Matappa Jurnal Kesehatan CERIA (Cerdas Energik Responsif Inovatif Adaptif) Jurnal Litbang Sukowati : Media Penelitian dan Pengembangan JURNAL PENDIDIKAN MIPA Jurnal PGSD Jurnal Ilmiah Kesehatan Journal of Stem Cell Research and Tissue Engineering Jurnal Vokasi Jurnal Pengabdian Dharma Bakti Jurnal Kajian Gender dan Anak Jurnal Metaedukasi : Jurnal Ilmiah Pendidikan Indonesia Natural Research Pharmaceutical Journal (INRPJ) Jurnal Aisyah : Jurnal Ilmu Kesehatan Bulletin of Counseling and Psychotherapy Abdimasku : Jurnal Pengabdian Masyarakat Jurnal Peduli Masyarakat AS-SABIQUN: Jurnal Pendidikan Islam Anak Usia Dini Journal Of Millennial Community Jurnal Abdidas Indonesian Journal of Global Health research Jurnal Pengabdian UNDIKMA Ideguru: Jurnal Karya Ilmiah Guru Civitas Ministerium: Jurnal Pengabdian Kepada Masyarakat Jurnal Terapan Ilmu Ekonomi Manajemen dan Bisnis (JTIEMB) MURHUM : JURNAL PENDIDIKAN ANAK USIA DINI Community Empowerment Otonomi IGKOJEI: Jurnal Pengabdian Masyarakat Teaching and Learning Journal of Mandalika (Teacher) Ceria: Jurnal Program Studi Pendidikan Anak Usia Dini Nakhoda: Jurnal Ilmu Pemerintahan CENDEKIA : Jurnal Ilmu Pengetahuan MAHESA : Malahayati Health Student Journal Archives Pharmacia JSAP : Journal Syariah and Accounting Public Dharmas Education Journal (DE_Journal) Riset, Ekonomi, Akuntansi dan Perpajakan (REKAN) Journal of Research in Instructional Journal of Early Childhood Education Jurnal Sistem Informasi, Teknik Informatika dan Teknologi Pendidikan (JUSTIKPEN) Jurnal Hukum Sehasen Journal of Office Administration : Education and Practice Pharmacy Action Journal Jurnal Basicedu Prosiding University Research Colloquium JURNAL AKUNTANSI DAN BISNIS Borobudur Counseling Review DEVOTE: Jurnal Pengabdian Masyarakat Global Jurnal Pendidikan Guru Matematika EJOIN : Jurnal Pengabdian Masyarakat Innovative: Journal Of Social Science Research Prosiding Seminar Informasi Kesehatan Nasional KAMI MENGABDI RENATA Jurnal Pengabdian Masyarakat Kita Semua Jurnal An-Najat: Jurnal Ilmu Farmasi dan Kesehatan Sosio Religi: Jurnal Kajian Pendidikan Umum Molekul: Jurnal Ilmiah Kimia Matrix: Jurnal Pendidikan Matematika Scientica: Jurnal Ilmiah Sains dan Teknologi JIM: Jurnal Ilmiah Mahasiswa Pendidikan Sejarah Tematik Indonesian Journal of Pharmaceutical Research Mopolayio : Jurnal Pengabdian Ekonomi Jurnal Pengabdian Kepada Masyarakat JECIE (Journal of Early Childhood and Inclusive Education) Jurnal Warna Management Studies and Business Journal Jurnal Riset Ilmiah Tuladha : Jurnal Pendidikan Dasar Indonesian Journal of Community Empowerment PANDAWA : Jurnal Pengabdian kepada Masyarakat Journal of Professional Elementary Education Jurnal Elementaria Edukasia Jurnal Terapan Pendidikan Dasar dan Menengah Journal of Community Services and Engagement: Voice of Community (VOC) Jurnal Adiguna Pengabdian Masyarakat JBK (Jurnal Bimbingan dan Konseling) Bhamada: Jurnal Ilmu dan Teknologi Kesehatan (E-Journal) Jurnal Cendekia Ilmiah JURNAL INOVASI PENDIDIKAN DASAR DAN MENENGAH JURNAL INSAN CENDEKIA JMAEKA: Jurnal Manajemen Ekonomi Akuntansi Jurnal DIMASTIK EDUKASI : Jurnal Penelitian dan Artikel Pendidikan Journal of Social Entrepreneurship and Creative Technology Folia Medica Indonesiana
Purwati Purwati
Institute Tropical Desease, Faculty Of Medicine/Airlangga University, Surabaya.
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Pengaruh Kesadaran Perpajakan Terhadap Ketidakpatuhan Pajak usaha Mikro Kecil Menengah dengan Pendekatan Psikologi Purwati, Purwati
Riset, Ekonomi, Akuntansi dan Perpajakan (Rekan) Vol. 6 No. 1 (2025): Riset, Ekonomi, Akuntansi dan Perpajakan (Rekan)
Publisher : Universitas Bumigora

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30812/rekan.v6i1.4839

Abstract

Pajak merupakan kontribusi wajib kepada negara yang bersifat mengikat dan harusdibayarkan oleh wajib pajak sesuai dengan peraturan yang berlaku, tanpa adanya imbalan langsung. Penelitian ini bertujuan untuk menguji serta menganalisis pengaruhkesadaran perpajakan terhadap ketidakpatuhan pajak UMKM di KPP Pratama Jepara.Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kuantitatif. Sampel penelitian terdiri dari sebagian wajib pajak, khususnya pelaku UMKMyang terdaftar di KPP Pratama Jepara, dengan jumlah sampel sebanyak 50 responden.Teknik pengumpulan data dilakukan melalui metode angket, dengan sumber dataprimer yang diperoleh langsung dari para responden. Data yang terkumpul dianalisismenggunakan regresi linier sederhana dengan SPSS 23. Hasil penelitian menunjukkan adanya hubungan antara kesadaran perpajakan dengan ketidakpatuhan pajakdi KPP Pratama Jepara, yang ditunjukkan oleh nilai t-hitung lebih besar dari t-tabel.Dengan demikian, penelitian ini memberikan hasil positif dan mendukung hipotesisbahwa terdapat pengaruh antara kesadaran perpajakan terhadap ketidakpatuhan pajakUMKM di KPP Pratama Jepara. Berdasarkan hasil penelitian, implikasi penelitianini memberikan masukan penting bagi berbagai pihak. Bagi pemerintah, khususnyaDirektorat Jenderal Pajak, hasil penelitian ini menyoroti perlunya peningkatan upayasosialisasi dan edukasi perpajakan yang lebih efektif, terutama bagi pelaku UMKM.
LEMAK BUKAN MUSUH: EDUKASI GIZI ANAK DAN REMAJA TENTANG LEMAK SEHAT FAT IS NOT THE ENEMY: NUTRITIONAL EDUCATION FOR CHILDREN AND ADOLESCENTS ABOUT HEALTHY FATS Purwati, Purwati
Pharmacy Action Journal Vol 4, No 2 (2025): 2025
Publisher : UNIVERSITAS 17 AGUSTUS 1945 JAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52447/paj.v4i2.8371

Abstract

AbstrakPersepsi yang keliru tentang lemak sebagai komponen nutrisi yang harus dihindari masih banyak ditemukan pada anak dan remaja. Tujuan dari kegiatan ini adalah memberikan edukasi gizi kepada anak dan remaja tentang pentingnya lemak sehat dalam pola makan sehari-hari. Kegiatan edukasi dilaksanakan di SMPN 95 Jakarta Utara dengan sasaran siswa kelas VII. Metode yang digunakan adalah edukasi interaktif melalui pemaparan materi, diskusi, dan evaluasi menggunakan pre-test dan post-test. Materi yang disampaikan meliputi pengertian lemak, jenis-jenis lemak, peran lemak dalam tumbuh kembang, serta sumber makanan yang mengandung lemak sehat. Hasil pre-test menunjukkan bahwa mayoritas peserta memiliki persepsi negatif tentang lemak dan kurang memahami peran penting lemak sehat bagi tubuh. Setelah edukasi, terjadi peningkatan signifikan pada hasil post-test, dengan peserta mampu membedakan jenis lemak sehat dan tidak sehat serta memahami fungsi vital lemak dalam pertumbuhan dan perkembangan. Kegiatan ini berhasil meningkatkan literasi gizi peserta dan mengubah persepsi negatif terhadap lemak menjadi pemahaman yang lebih seimbang. Direkomendasikan agar edukasi gizi sejenis dilakukan secara berkelanjutan dengan metode yang lebih beragam untuk memperluas dampak positifnya.Kata Kunci: edukasi gizi, lemak sehat, literasi gizi, remaja, anak-anak Abstract Misconceptions about fat as a nutritional component that should be avoided are still commonly found among children and adolescents. The purpose of this activity was to provide nutritional education to children and adolescents about the importance of healthy fats in their daily diet. The educational activity was conducted at SMPN 95 North Jakarta with seventh-grade students as the target audience. The method used was interactive education through material presentation, discussion, and evaluation using pre-test and post-test. The material covered included the definition of fat, types of fat, the role of fat in growth and development, and food sources containing healthy fats. Pre-test results showed that the majority of participants had negative perceptions about fat and lacked understanding of the important role of healthy fats for the body. After the education, there was a significant improvement in the post-test results, with participants able to distinguish between healthy and unhealthy types of fat and understand the vital function of fat in growth and development. This activity successfully increased the participants' nutritional literacy and changed negative perceptions of fat into more balanced understanding. It is recommended that similar nutrition education be conducted continuously with more diverse methods to expand its positive impact.Keywords: nutrition education, healthy fats, nutrition literacy, adolescents, children
Uji Aktivitas Repellent Sediaan Gel Ekstrak Etanol Daun Belalai Gajah (Clinacanthus nutans) Terhadap Nyamuk Aedes aegypti Jayadwipa, Muhammad Waru Jayadwipa; Purwati, Purwati
An-Najat Vol. 3 No. 1 (2025): An-Najat: Jurnal Ilmu Farmasi dan Kesehatan
Publisher : STIKes Ibnu Sina Ajibarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59841/an-najat.v3i1.1752

Abstract

Demam berdarah merupakan menyakit yang disebabkan oleh virus dengue yang ditularkan oleh nyamuk Aedes aegypti melalui gigitan. Berdasarkan data dari kemenkopmk pada periode januari sampai juli 2023, terdapat sebanyak 42.920 orang yang terinfeksi DBD dan 317 orang meninggal. Di indonesia, kebanyakan masyarakat masih menggunakan repellent nyamuk berbahan kimia, yaitu DEET. Untuk mengurangi penggunaan bahan kimia, disarankan untuk menggunakan repellent nyamuk yang menggunakan bahan utama alami seperti ekstrak etanol daun belalai gajah. Tujuan dari penelitian ini untuk mengetahui apakah gel yang mengandung ekstrak etanol daun belalai gajah dapat menjadi repellent yang baik atau tidak untuk nyamuk Aedes aegpyti. Evaluasi fisik sediaan gel terdiri dari uji organoleptis, uji homogenitas, uji pH, uji viskositas, uji daya sebar yang disimpan selama 8 minggu dan uji iritasi selama 24 jam. Pengujian aktivitas repellent terhadap nyamuk Aedes aegpyti dilakukan dengan cara pengolesan gel ekstrak etanol terhadap 5 probandus dan dimasukan ke dalam kandang berisi 50 nyamuk betina secara bertahap. Banyaknya nyamuk yang hinggap pada probandus dihitung mulai dari jam ke-0 hingga jam ke-4 sebanyak 5x setiap jamnya, dan setiap pengulangan dilakukan selama 5 menit. Konsentrasi ekstrak etanol daun belalai gajah yang dipakai 0.3%;0.6%;1% memiliki nilai persen daya proteksi berturut-turut sebesar 91.2%;93.14%;94.63% serta memberikan efek yang signifikan (p<0,05). Hasil penelitian menunjukan bahwa formulasi III dengan konsentrasi 1% ekstrak etanol daun belalai gajah memberikan daya perlindungan paling baik dari ketiga formulasi.
INTERAKSI RESIPROKAL OTAK DAN PERILAKU PADA PERKEMBANGAN ANAK Santana, Selly Aprilia; Kusumah, Tyara Intana Putri; Purwati, Purwati
CENDEKIA: Jurnal Ilmu Pengetahuan Vol. 5 No. 2 (2025)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/cendekia.v5i2.4851

Abstract

The golden period in early childhood is a critical phase in the development of the child's brain, where the neural foundation is intensively formed through experience, social interaction, and parenting patterns. This study aims to examine the reciprocal relationship between brain development and behavior in early childhood and how parenting patterns affect these dynamics. The method used is a literature study with a descriptive qualitative approach, carried out through data collection and secondary data analysis from relevant scientific journal articles. The results of the study indicate that brain function structures such as the prefrontal cortex, amygdala, and hippocampus have an important role in the development of children's behavior and emotional regulation. The development of the prefrontal cortex contributes to the ability to manage emotions, focus attention, and demonstrate children's behavior that is in accordance with social norms. Maladaptive behavior in early childhood that arises due to negative parenting patterns can interfere with and damage the development of brain tissue, especially in the memory and self-control centers, thereby inhibiting healthy brain circuits in children. In conclusion, there is a reciprocal relationship between the brain and children's behavior, where positive, responsive, and consistent parenting plays a major role in shaping healthy neural development in early childhood. This study emphasizes the importance of parenting intervention approaches that support optimal child brain development. ABSTRAKPeriode keemasan pada anak usia dini merupakan fase kritis dalam perkembangan otak anak, di mana fondasi neural dibentuk secara intensif melalui pengalaman, interaksi sosial, dan pola pengasuhan. Penelitian ini bertujuan untuk mengkaji hubungan resiprokal antara perkembangan otak dan perilaku anak usia dini dan bagaimana pola pengasuhan memengaruhi dinamika tersebut. Metode yang digunakan adalah studi literatur dengan pendekatan kualitatif deskriptif, dilakukan melalui pengumpulan data dan análisis data sekunder dari artikel jurnal ilmiah yang relevan. Hasil kajian menunjukkan bahwa struktur fungsi otak seperti prefontal cortex, amigdala, dan hippocampus memiliki peran penting dalam perkembangan perilaku dan regulasi emosi anak. Perkembangan prefontal cortex berkontribusi terhadap kemampuan mengelola emosi, fokus perhatian, serta menunjukkan perilaku anak yang sesuai dengan norma sosial. Perilaku maladaptif anak usia dini yang muncul akibat pola asuh negatif dapat mengganggu dan merusak perkembangan jaringan otak terutama pada pusat memori dan pengendalian diri, sehingga menghambat sirkuit otak anak yang sehat. Kesimpulannya, terdapat hubungan timbal balik antara otak dan perilaku anak, di mana pengasuhan yang positif, responsif, dan konsisten sangat berperan dalam membentuk perkembangan neural yang sehat pada anak usia dini. Penelitian ini menekankan pentingnya intervensi pendekatan pengasuhan yang mendukung perkembangan otak anak secara optimal.  
Systematic literature review: Dampak bentakan terhadap kerusakan otak dan perilaku anak Silvi Pebrianti Senjawiani, Silvi; Rifda Sa'adatul Laila, Rifda; Purwati, Purwati
CERIA (Cerdas Energik Responsif Inovatif Adaptif) Vol. 8 No. 3 (2025): Volume 8 Number 3, May 2025
Publisher : IKIP Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22460/ceria.v8i3.27092

Abstract

Shouting is often used as a disciplinary method for children, but it can negatively affect their brain development and behavior. In response, this study aims to identify the impact of shouting and explore preventive strategies to mitigate brain damage and behavioral issues in children. A systematic literature review (SLR) method was employed to answer the research questions in a structured manner, following the PRISMA 2020 guidelines. A total of 100 articles were reviewed using the keywords (1) shouting, (2) shouting at children, and (3) the impact of shouting on the brain and behavior. The literature search was conducted using EndNote X9 and assisted by Harzing’s Publish or Perish (PoP), resulting in 10 relevant articles sourced from the Google Scholar database. Data collection involved literature screening and document analysis, followed by content analysis using a thematic qualitative descriptive approach. The results revealed that shouting may impair children's cognitive development (such as memory and concentration), emotional well-being (increased anxiety, stress, and sadness), and behavior (including aggression, withdrawal, and low self-esteem). As preventive measures, the study highlights the importance of loving parenting, gentle communication, positive learning environments, and early intervention. These findings underscore the necessity of adopting positive parenting and educational approaches to support optimal mental health and brain development in early childhood
Personality Competence of Counselors in Guidance and Counseling Services at Junior High Schools in Magelang Lianasari, Dewi; Purwati, Purwati
KONSELI: Jurnal Bimbingan dan Konseling (E-Journal) Vol 9 No 1 (2022): KONSELI : Jurnal Bimbingan dan Konseling (E-journal)
Publisher : Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/kons.v9i1.9662

Abstract

The success of the implementation of guidance and counseling services in schools is determined by one of the personality competencies possessed by the counselor. Personal competency standards are one part of the counselor competency standards. Counselor competency standards are minimum standards that must be known, achieved and performed proficiently by counselors. The purpose of this study was to produce an instrument in the form of a counselor personality competency scale. This study uses a Research and Development design up to the product trial stage on a limited basis. The data collection instrument used a personality competency scale. The results showed that using the personality competency scale that had been tested, it was seen that 97% were in the medium category and 3% were in the high category.
The effect of reality therapy group counseling with the WDEP technique on increasing student academic resilience Afifah, Hasna Nur; Purwati, Purwati; Putro, Hijrah Eko
KONSELI: Jurnal Bimbingan dan Konseling (E-Journal) Vol 9 No 2 (2022): KONSELI : Jurnal Bimbingan dan Konseling (E-journal)
Publisher : Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/kons.v9i2.12984

Abstract

As a student, many demands are imposed, starting from the needs of academic and non-academic values, participating in various intra and extracurricular activities to add value and expertise, fibre and much more. From the many demands that exist, students often experience academic stress. For this reason, high academic resilience is needed to cope with academic stress. The purpose of this study was to examine the effect of group counselling with the WDEP technique to improve students' academic resilience. This WDEP technique is one of the techniques in reality therapy which is an acronym for Want, Doing and Direction, Evaluation and Planning. The research was conducted on grade VII G students at SMP Negeri 3 Magelang. Pre-experiment, using one group pre-test post-test design. Where the design uses one group that will be used as an experimental group to be given treatment in the form of WDEP technique group counselling services. By using a purposive sampling technique, from 32 students in the class, 8 students were taken as members of the experimental group to participate in group counselling activities. Meanwhile, the method for analyzing the data used parametric statistics paired sample t-test with SPSS 25 software. The results of the study show that the WDEP technique of group counselling affects increasing students' academic resilience.
Pengaruh Persepsi Harga terhadap Kepuasan Pelanggan di Mie Gacoan Way Halim Bandar Lampung Purwati, Purwati; Putra, Tedy Ramanda; Kalistania, Kalistania; Rosalino, Aradia; Rahmawati, Rahmawati
Jurnal Pendidikan Tambusai Vol. 9 No. 2 (2025): Agustus
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai, Riau, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jptam.v9i2.30462

Abstract

Perkembangan sektor kuliner menghadapi tantangan berupa kurangnya inovasi dalam pengembangan produk dan persepsi harga untuk menarik minat pelanggan. Perkembangan sektor kuliner yang pesat di tengah persaingan harga dan kualitas membuat perusahaan seperti Mie Gacoan perlu memahami sejauh mana persepsi harga berpengaruh terhadap kepuasan pelanggan. Penelitian ini bertujuan untuk mengetahui pengaruh persepsi harga terhadap kepuasan pelanggan di Mie Gacoan Way Halim Bandar Lampung. Penelitian ini menggunakan jenis penelitian kuantitatif dengan menggunakan metode deskriptif verifikatif, pendekatan ex post facto dan survey. Sampel yang digunakan sebanyak 105 responden, diperoleh melalui rumus incidental sampling dari populasi sebanyak 210 orang pelanggan Mie Gacoan. Tahapan analisis data yang dilakukan pada penelitian ini adalah dengan uji persyaratan instrumen meliputi uji validitas (pearson product moment correlation), uji reliabilitas (Alfa Croncbach); uji persyaratan analisis data meliputi, uji normalitas (kolmogorov-smirnov), uji homogenitas(Levene statistic); uji asumsi klasik meliputi uji linearitas (Ramsey test), uji multikolinearitas (TOL-VIF), uji heteroskedastisitas (Rank spearman), uji autokorelasi (Durbin-Watson); uji hipotesis meliputi uji regresi dan korelasi berganda. Hasil penelitian menunjukkan bahwa persepsi harga berpengaruh signifikan terhadap kepuasan pelanggan, dengan nilai t-hitung sebesar 13,904 dan signifikansi sebesar 0,000, serta nilai R² sebesar 0,652 (65,2%). Dengan demikian, persepsi harga berpengaruh positif terhadap kepuasan pelanggan.
Optimization and Characterization of Urease Immobilization from Red Lentil Seeds (Lens culinaris) Using Chitosan zusfahair, zusfahair; Ningsih, Dian Riana; Bilalodin, Bilalodin; Fatoni, Amin; Luthfia, Adilla; Purwati, Purwati; Muslihah, Niken Istikhari; Apriliadina, Inessa Putri
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13134

Abstract

ABSTRACT. Urease is an enzyme that plays a vital role in catalyzing the hydrolysis of urea into ammonia (NH3) and carbon dioxide (CO2). This study focuses on the isolation of urease from red lentil seeds, followed by its immobilization. The objective of this research is to optimize and characterize urease that has been immobilized using chitosan and activated with glutaraldehyde. Red lentil seeds were processed with a mortar and pestle at low temperatures (4 °C) to obtain a crude enzyme extract, which was then concentrated using 50% acetone (P50) prior to immobilization. The optimization process for P50 urease immobilization involved assessing various factors, including chitosan concentration, glutaraldehyde concentration, temperature, and the immersion duration in glutaraldehyde. The findings revealed that the optimal conditions for immobilizing P50 urease were achieved at a chitosan concentration of 0.75%, with a 2% glutaraldehyde soak at 25 °C for 2 hours, resulting in an enzyme activity of 7.042 U/g. The immobilized P50 urease demonstrated the ability to be reused up to 7 times while maintaining 51% of its initial activity. Scanning Electron Microscopy (SEM) analysis indicated morphological changes in the beads after the addition of glutaraldehyde and the enzyme, shifting from a rounded to an irregular shape. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) analysis identified C-N and C=N peaks, confirming the successful incorporation of glutaraldehyde. Keywords: immobilization, red lentil seeds, glutaraldehyde, chitosan, urease
PROTOTYPE DESIGN OF LYMPHOCYTE TCD4+ RESISTANT AGAINST HIV INFECTION GENERATED FROM PERIPHERAL BLOOD HAEMATOPOIETIC STEM CELL (PBMCs) By DELETION OF 32 bp CCR5 ENCODING GENE Purwati, Purwati
Journal of Stem Cell Research and Tissue Engineering Vol. 1 No. 1 (2017): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.708 KB) | DOI: 10.20473/jscrte.v1i1.7566

Abstract

AIDS epidemic has spread to all parts of Indonesia and currently more than 150 countries reported the existence of HIV/AIDS from around the world. Additionally, HIV/AIDS treatment using ARV drugs also find obstacles that must be faced in terms of host, environment and the agent. The objective of this study was to generate lymphocytes TCD4+ that are resistant to HIV infection generate from PBMCs through by deletion of 32 bp CCR5 encoding gene. In principle, this study was done in three steps. First, isolation, culture and purification of lymphocyte TCD4+ from PBMC (Mather, 2008; Rantam, et.al., 2009). Second, lymphocyte TCD4+ characterization by PCR with primer F 5'CAAGTCGAGCGCCCCGCAAGGGG-3, R 5'GTCCGAGTGTGGCTGATCATCC-3 (Thomsen, et.al., 2002; Yuwono, 2006; Hall and Ziedonis 2007; Purwati, et.al., 2009). Third, designing of lymphocyte TCD4+ prototype which was resistant to HIV infection by deletion of 32 bp CCR5 full gene. Results: Twenty-four hours after culture, there were abundant cell growths. TCD4+ lymphocytes from isolated and cultured 10 ml PBMC were found to be 2 x 107. Phenotype characterization of TCD4+ lymphocyte provided positive results, while the genotype showed similarities to that in corresponding gene bank of CCR5 variant A and variant B. Prototype of HIV resistant TCD4+ lymphocytes was made by nucleotide deletions in conserved areas, at position 554-576 bp, using restriction enzymes EcoRI checked using PCR and sequencing. In conclusion, prototype design of HIV resitent TCD4+ lymphocytes is obtained through the deletion of 32 bp CCR5 encoding full gene at GTCAGTATCAATTCTGGAA GAATTT CCAGACA using EcoRI enzyme.Keywords: HIV/AIDS, resistant TCD4+ lymphocytes, mutant 32 bp CCR5, PBMCs, deletion
Co-Authors A. Syachruroji, A. A.A. Ketut Agung Cahyawan W Abdul Hakam Ace Suryadi Adi Saputra Adrianto Prasetyo Perbowo Aerin, Wafa Affandi, Ahmad I Afiani, Ardita Afifah, Hasna Nur Afikah, Hana Tri Afriyanni Afriyanni, Afriyanni Agusrina, Yohana Agustanti, Dwi Agustin, Hanum Widyanti Agustina Pujiwati, Lina Agusyani Agusyani, Agusyani Ahmad Hamidi Ahmad Rifai Airlangga, Primadenny Ariesa Al Hafidz, Muhammad Alelo, Richardo Reynaldi Sakka Alfi Noviyana, Alfi Alfitriani, Alfitriani AMALIA, MEGA AMIN FATONI Amirullah, Fajar Amperaningsih, Yuliati Ana Fitrotun Nisa Andi Fajeriani Wyrasti Andriani, Dwi Nila Andriani, Meri Andriyani , Titi Andriyani, Titi Andriyanti, Siti Dwi Angely Septihany, Elisabeth Anita Anita Annamayra, Ajeng Antoni, Febry Yanto Aprih Santoso Apriliadina, Inessa Putri Apriliani, Happy Dwi Aprina, Aprina Ardani, Annas Yudi Arhesa, Sandra Arif Sanjaya, Muhammad Arif Zulkarnain Arif Zulkarnain Aris Dwi Cahyono, Aris Dwi Arliandari, Anggita Asih, Susmawati Surya Astagini, Virda Anggi Astriyanto, Febri Kalingga Azzahra, Arwa Bakhri, Rafdlal Saeful Benidiktus Tanujaya Bilalodin Bilalodin Budiarni, Kasandra Budiarti, Dhiani Cahyo, Ferdiawan Diki Camalia, Rafika Sita Chaeroni, Ahmad Chrisna Sujatmiko Clara, Clara Damayanti, Debi Cahya Darisman, Dede Darmawan, Agit Dwi Darwati, Lestari Eko Darwis, Rahmiati Dermawan, Irwan DEWI SARTIKA Dewi, Amelia Dewi, Sawitri Dhini Putri Aprilia Dian Riana Ningsih Dian Sandri Djumrianti, Desloehal Dwi Nila Andriani Dwi Nilla Andriani Dwikora Novembri Utomo Ebeneser Wacner Simamora Eka Syafitri, Siska Eko Slamet, Eko Elan Elan, Elan Elawati, Nur Elidar, Yetti Elza Reihana Emaya Kurniawati, Emaya Encep Andriana Encep Syarief Nurdin, Encep Syarief Endi Prilansa Mahadi Esya Alhadi Fadila, Adelia Nur Fadilla, Reza Noor Fadli, Nur Annisa Faiz, Aiman Faizah, Rayinda Fajarwati, Evi Fatubun, Rainita Fatubun, Rainitha Fedik Abdul Rantam Ferdiansyah Mahyudin Filda Khoirun Nikmah Firda Amalia, Nurul Firmansyah Firmansyah Fobia, Maranatha Lisatyaningrum Hainekam Galih Istiningsih, Galih Gandana, Gilar Gevisioner Gevisioner Gevisioner, Gevisioner Ghassani, Fatiha Azka Gistiani, Elsa Gita Febria Friskawati Guanse, Yovi Guntara, Rangga Gelar Gusni, Pilda Hak, Mohammad Hafid Halimah, Cita Hermitha Noor Hamka Hamka, Hamka Handini, Mayang Ayudya Hani Irhamdessetya Hanim, Abra Uzri Haryanto Haryanto Hasbullah, Zahra Rahmawati Hayati, Kemala Hendra Sastrawinata Hendra Wijaya Hendri Busman Hermawan, Rirsdianto Hidayanti, Agil Hijrah Eko Putro I Wayan Yudiana Ikhtiarawati, Irin Febry Ilmaa, Miftahul Imas Kurniawaty Ina, Ina Indah Dwi Wahyuni, Indah Dwi Indah Novita Sari Indah Novita Sari Indiati Indiati, Indiati Indra Lesmana Irma Dewi Isda Irnandi, Irfan Iskandar AM, . Iskandar, Budi Izzatunnisa, Nazwa Jayadwipa, Muhammad Waru Jayadwipa Kalistania, Kalistania Karman Lanani Karomah, Siti Karomah, Siti Kartikasari, Annisa Yuli Khoerunnisa, Anis Kurnia, Amanatul Aulya Kurniati, Astiwi Kusdinar, Yopi Kusumah, Tyara Intana Putri Kusumastuti, Anggit Dyah la nani, karman Lailatusaadah, Nuke Laksono Trisnantoro Langit, Vinsensia Asahi Sulan Latifatunnisa, Ayu Lenoera, Agnes Lianasari, Dewi Lianasari, Lianasari Loita, Aini Lukas Widhiyanto Lusi, Lusi Luthfia, Adilla M. Syahirman Yusi Maghfiroh, Rizki Malik, Arief Abdul Malik, Ririn Purwati Marchelia, Laili Zahrina Mardiyah Kurniasih Marieska Lupikawaty Mariskha Z Markoni Badri Marwia Tamrin Bakar Maulana Rizqi, Anggi Melati, Nadhira Tasya Milat, Karima Miskiyah, Neneng Mohamad, Fajar Muslim Moniqa Siagawati, Moniqa Muchamad Arif Al Ardha Muhammad Hidayat Muhammad Jafar, Muhammad Muhammad Japar Muhammad Japar Mulyadi, Sima Sima Mulyana, Edi Hendri Mumu, Jeinne Munthe , Ibnu Rasyid Murhan, Al Murtiningsih Murtiningsih Musa’adah, Silpi Muslihah, Niken Istikhari Mz, Fannia Khairani Naimah, Hidayatun Nanang Ari Utomo, Nanang Ari Nasronudin Nasronudin Naufal Haidar Ahmada Ngurah Ayu Nyoman M Nilan, Fariha Ningsih Ismi, Dian Ayu Nizar Saputra Nofi Nur Yuhenita Novi Trisnawati Noviana, Tiara Nugraha, Muhammad Rizki Nugroho, Agung Yuliyanto Nulhakim, Lukman Nur Alim Natsir Nur Hikmah Nur, Lutfi Nuraini, Paramita Nurcahyo, Satria A Nurkolis Nurkolis, Nurkolis Nurul Fauziah Nurwasila, Nurwasila Nurwijayanti Ode, Abdul Syukur Oktarina, Lia Oktaviana, Sovia Omry Tri Asmara Adi Opur, Hamka George Marly Hairil Paidah, Egia Juliatul Pangadongan, Sri Putri Panjaitan, Riong seulina Pebrianti, Yulia Prabowo, Suluh Agung Prasetio, Iradah Lia Pratama, Peby Ardiani Pratama, Rohmawan Adi Pratito, Dwi Widi Pratiwi, Mutia Diah Prawitmo, Albertus Purbandini, Revi Aulia Purnamasari, Mita Purwati, Alfi Noviyana Puspita Rachma, Ulfa Putra, Tedy Ramanda Qodri, Laila QONITA Rahmadi, Antun Rahman, Alisyah Rahmasari, Ikrima Rahmawati, Ocha Tria Rahmawati, Rahmawati Rakhmat, Cece Ramadhani, Dini Ratna Dewi Mulyaningtiyas Ratna Stia Dewi Rauzika Altasa, Fiza Resi, Resi Wulansari Restu Kusumaningrum, Nugraheni Rianti, Yuli Ridhoni, Muhammad Rifda Sa'adatul Laila, Rifda Rifki, Zulfa Zuyyina Rifqi Ilhamul Fauzi, Muhamad Rikana, Heppy Rinawati, Tri Rizal, Ahmad Symsu Rizqi, Anggi Maulana Rofiqoch, Isnaeni rohayati rohayati Rohimah, Nala Siti Rohmawan Adi Pratama Rohmayanti Rohmayanti Ronaldo, Geri Roni Prasetyo, Roni Rosalino, Aradia Sabilal Alif Sagala, Zuraida Salsabila, Siti Rahma Anissa Salsabilla, Zuli Astuti Sandha Soemantri, Sandha Santana, Selly Aprilia Saputra, Pebriansyah Sayuti, Jalaludin Septi Wardani, Septi Septiyati Purwandari Setiyo Prihatmoko Setyawan, I Gede Yudia Setyawati, Rossy Seulina Panjaitan, Riong Shofia, Dina Sholikah, Mar'atus Sianturi, Risbon Sihombing, Volvo Sila, Intan SILVANA OKTANISA, SILVANA Silvi Pebrianti Senjawiani, Silvi Sima Mulyadi, Sima Simatupang, Angel Maria Siregar, Nahrun Najib Siswahyudianto Siti Aisyah Soleh, Bukhori Solekha, Siti Soleman Saidi, Soleman Sonata, Alfiana Sonia Ningsih, Siska Sri Wulandari Sucipto, Muhammad Hardwin Adi Sudiyono Sudiyono Sugiyadi, Sugiyadi Sugiyadi, Sugiyadi Suhana, Ratna Azshari Sukoco, Hendro Sulastri Sulastri Sulistryaningtyas, Reza Edwin Sumaryanto Sumaryanto, Sumaryanto Sunanto Sunanto Sunaryo Hardjowijoto Sundawan Salya Supit, Tesalonica Maria Suratmi, Lily Surya Asih, Susmawati Suryo, Nabila Dwi Sutriningsih, Sutriningsih Syafitri, Siska Eka Syafri, Herman Syahbeni, Anwar Taha, Ikhsan Tahira, Anisa Taopik Rahman Teguh Ariefiantoro, Teguh Teten Hidayat, Teten Theodorus Rexa Handoyo Titi Purbo Sari Tri Candra Wulandari Tri Harningsih, Tri Tri Lestari Tri Wahyuningsih Ulfah, Sofia Ummasyroh Ummasyroh Utomo, Endrat Kartiko Veronica Kinanthi Sihutami Virdiana, Almaida Isnainita Wahdina, Wahdina Wahyuni, Anissa Wardani, Fitri Esti Wardani, Fitri Esti Wardani, Fitria Esti Wardani, Fitria Esti Wardani, Nila Ayu Kusuma Wigestari, Rona Willy Sandhika Wiratmoko, Wien Wironegoro, Rio Wiwin Dinar Prastiti, Wiwin Dinar Wulansari, Siska Devi Yanih, Satya Cangra Indra Yosepin, Elpina Yuliana, Efi Eka Yuniarti, Rima Yuniastini, Yuniastini Yusleli Herawati Yusuf, Mohammad Andi Yuwani, Hani Utin Zahidah, Izmi Khalda Zahro, Santika Fatimah Zakiyah*, Syifa Siti Zulida, Evi Zusfahair Zusfahair