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Tri Handayani
Universitas Padjadjaran
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Journal : JURNAL BIOLOGI INDONESIA

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Induksi dan Karakterisasi Pisang Mas Lumut Tetraploid Poerba, Yuyu s; ,, Witjaksono; Ahmad, F.; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.051 KB) | DOI: 10.14203/jbi.v10i2.2082

Abstract

Triploid banana breeding could be achieved by crossing tetraploid to diploid banana cultivars. The availaibility of tetraploidbananas is naturally very limited. One of methods to produce tetraploid bananas is inducing tetraploid plants usingchemicals that inhibit spindle fiber formation during mitosis such as oryzalin or colchicine. The present research is aimed toproduce and characterize tetraploid Pisang Mas Lumut (Musa acuminata, AAAA genome) induced by in-vitro oryzalintreatment from diploid Pisang Mas Lumut. Fifteen in vitro-cultured shoots were treated wih oryzalin at of 60μM for 7days in a liquid MS basal medium with addition of 2 mg/l BA. After 6 sub-cultured, 54 shoots and 104 plantlets wereproduced. Forty seedlings were then survived and grew well and healthy. Ploidy identification of induced Pisang MasLumut was conducted using Flowcytometer at seedling stage and repeated at reproductive phases for 2 cycles. The resultsshowed that oryzalin treatments produced tetraploids at a frequency of 30%, diploid at 65% and mixoploid at 5%. Thetetraploid plants have longer and thicker pseudostems, produce fewer suckers, drooping and larger leaves, and broader fruitscompared to its diploid control at harvest time. The tetraploid plants have similar genetic properties with their diploidcontrols as shown by genetic identity of 0.90-0.97.Key words: Pisang Mas Lumut, Musa acuminata, AA, oryzalin, tetraploid
Uji Toleransi Tanaman Kentang Hitam (Plectranthus rotundifolius (Poir.) Spreng.) Hasil Radiasi Sinar Gamma terhadap Cekaman Kekeringan Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2310

Abstract

Plectranthus rotundifolius (Poir.) Spreng. is one of the potential root crops to become an alternative food. The aim of thisstudy was to determine drought resistant status of Plectranthus rotundifolius irradiated by gamma irradiation. Thisexperiment was conducted in the Green House using a Completely Randomized Design with 2 factors and 5 replications.The first factor was plant number consisted of 7 accessions (D116, D69, M343, D40, M95, D3, and Klefa Imut/KI). Thesecond factor was the level of field capacity (FC) consisted of 100% FC, 60% FC, and 20% FC. The observed parameterwere vegetative and generative growth stage, stomatal conductance, leaf water potential, and chlorophyll content. The resultshowed that the resistance level of the 7 accessions can be devided into 3 categories: 1) The plants that were susceptible todrought i.e. D116, D69, M343, and KI; 2) The plants that were semi-tolerant to drought i.e. D40 and M95; and 3) The plantthat was tolerant to drought i.e. D3.Keywords: Plectranthus rotundifolius, gamma irradiation, drought stress
Induksi Tetraploid Pada Tanaman Jambu Biji Merah (Psidium guajava L.) secara In Vitro Handayani, Tri; Witjaksono, Witjaksono; Nugraheni, K. Utami
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (909.481 KB) | DOI: 10.14203/jbi.v13i2.3401

Abstract

ABSTRACTSome commercial varieties of Guava seedless are triploid and rarely found naturally. A triploid variety with less seeds and better yield potential can be achieved by crossing tetraploid to diploid variety. Guava tetraploid plants can be synthetically induced by using oryzalin or colchicine to double the chromosome from its diploid. This research was aimed to obtain tetraploid lines by studying the effects of oryzalin in germination and in vitro growth of Guava. Seed from Red Guava were cultured in liquid medium MS + 2 mgL-1 BA with adding oryzalin according to the treatment, then seed planted in MS solid medium. Seed explants were exposed to Oryzalin 0 (controls), 15, 30 dan 60 ?M with exposure time are 23, 36 and 49 days or 3, 5, and 7 weeks. Results from polyploid induction were 8 tetraploid (5.48%) and 9 (6.16%) mixoploid shoots in vitro. High est tetraploid shoots were obtained from treatments by exposing the seed explant to 15 – 30 ?M oryzalin for 23 – 36 days. Oryzalin treatments inhibit germination and in vitro growth of Guava until 1st subculture. After second subculture, tetraploid or mixoploid shoots quantitatively showed no difference respons on in vitro growth with its diploid.Keywords: guava, tetraploid, seed explant, in vitro germination, polyploid
Pembentukan dan Penampilan Pisang Rejang Hibrid Triploid Hasil Persilangan Pisang Rejang Mixoploid Dengan Pisang Rejang Diploid Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Abstract

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids
Karakterisasi Pisang Hibrid Tetraploid ‘GRNK’ Hasil Persilangan Pisang Triploid ‘Goroho’ dengan Pisang Iiar Diploid Musa acuminata Colla var. nakaii Nasution Poerba, Yuyu S.; Martanti, Diyah; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2794.062 KB) | DOI: 10.14203/jbi.v13i2.3393

Abstract

ABSTRACTThe use of tetraploid banana in triploid banana breeding has been conducted through 4x x 2x crossing and/or its reciprocals, 2x x 4x crossing. However, the availability of tetraploid banana is naturally very limited, therefore artificially induced tetraploid banana is needed. The present research is aimed to generate tetraploid banana plants by crossing triploid banana ‘Goroho’ as female parent with wild diploid banana, Musa acuminata var. nakaii Nasution as male parent, and to characterize the tetraploid hybrid. 94 crossings were made by pollinating stigma of Pisang Goroho with polen of M. acuminata var. nakaii. Embrios resulted from hybridization were in vitro rescued and propagated. Hybrid plantlets were then acclimatized in the green house and seedlings were planted in the field for two cycles of banana reproduction. Ploidy identification, morphology and molecular characteristics were also conducted for two cycles of banana reproduction. Results of the experiment showed that 9 hybrid seeds were produced from 94 pollinations. However, only 2 hybrid embryos grew and developed into shoots and from two shoots only one shoot was survived. The one shoot were then in-vitro propagated to produce 21 hybrids planted in the field. After being evaluated for two plant reproductive cycles, all hybrid plants were tetraploids (2n=4x=44). The hybrid plants had drooping leaves, bigger fruit diameter compared to both parents, and blunt fruit tip. DNA (RAPD) profiles of tetraploid hybrid showed that 37.5% of the bands were from female parent, 37.5% from male parents, and 25% from both parents. The tetraploid hybrid ‘GRNK’ is expected to be useful in triploid banana breeding in the future.Key Words: Hybrid banana, tetraploid, Goroho, Musa acuminata Colla var. nakaii Nasution
Respon Tanaman Jewawut [Setaria italica (L.) P. Beauv.] terhadap Kondisi Cahaya Rendah Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.759 KB) | DOI: 10.14203/jbi.v14i1.3656

Abstract

ABSTRACTMillet [Setaria italica (L.) P. Beauv.] is one of the potential cereal crops to be developed in supporting food diversification program. The purpose of this study was to investigate the response of millet to low light (shade) condition. The experiment was conducted in the field with nested design that consisted of two factors that are, 1) Accession from Buru Island which are yellow seed and red seed; and 2) Shading nets that consisted of 0%, 40%, and 70% shade. Shade factor was designed as main plot, while accession factor was designed as sub plot. Growth (growing percentage, plant height, leaf number, leaf area, and plant dry weight), yield (panicle length and panicle weight), photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid), and stomata density of the millet were observed. The result showed that the growth and yield of both accession were decreased by the increase of shading. However, yellow seed millet tended to be more tolerant than red seed millet with optimizing photosynthetic capacity through enhancing leaf area, leaf longevity, and enhancing antenna pigments such as chlorophyll b and carotenoid.Keywords: Growth, millet, photosynthetic pigments, shading, yield
INDUKSI DAN KARAKTERISASI PISANG MAS LUMUT TETRAPLOID Poerba, Yuyu s; ,, Witjaksono; Ahmad, F.; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2082

Abstract

Triploid banana breeding could be achieved by crossing tetraploid to diploid banana cultivars. The availaibility of tetraploidbananas is naturally very limited. One of methods to produce tetraploid bananas is inducing tetraploid plants usingchemicals that inhibit spindle fiber formation during mitosis such as oryzalin or colchicine. The present research is aimed toproduce and characterize tetraploid Pisang Mas Lumut (Musa acuminata, AAAA genome) induced by in-vitro oryzalintreatment from diploid Pisang Mas Lumut. Fifteen in vitro-cultured shoots were treated wih oryzalin at of 60μM for 7days in a liquid MS basal medium with addition of 2 mg/l BA. After 6 sub-cultured, 54 shoots and 104 plantlets wereproduced. Forty seedlings were then survived and grew well and healthy. Ploidy identification of induced Pisang MasLumut was conducted using Flowcytometer at seedling stage and repeated at reproductive phases for 2 cycles. The resultsshowed that oryzalin treatments produced tetraploids at a frequency of 30%, diploid at 65% and mixoploid at 5%. Thetetraploid plants have longer and thicker pseudostems, produce fewer suckers, drooping and larger leaves, and broader fruitscompared to its diploid control at harvest time. The tetraploid plants have similar genetic properties with their diploidcontrols as shown by genetic identity of 0.90-0.97.Key words: Pisang Mas Lumut, Musa acuminata, AA, oryzalin, tetraploid
PEMBENTUKAN DAN PENAMPILAN PISANG REJANG HIBRID TRIPLOID HASIL PERSILANGAN PISANG REJANG MIXOPLOID DENGAN PISANG REJANG DIPLOID Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Abstract

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids
RESPON TANAMAN JEWAWUT [SETARIA ITALICA (L.) P. BEAUV.] TERHADAP KONDISI CAHAYA RENDAH Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3656

Abstract

ABSTRACTMillet [Setaria italica (L.) P. Beauv.] is one of the potential cereal crops to be developed in supporting food diversification program. The purpose of this study was to investigate the response of millet to low light (shade) condition. The experiment was conducted in the field with nested design that consisted of two factors that are, 1) Accession from Buru Island which are yellow seed and red seed; and 2) Shading nets that consisted of 0%, 40%, and 70% shade. Shade factor was designed as main plot, while accession factor was designed as sub plot. Growth (growing percentage, plant height, leaf number, leaf area, and plant dry weight), yield (panicle length and panicle weight), photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid), and stomata density of the millet were observed. The result showed that the growth and yield of both accession were decreased by the increase of shading. However, yellow seed millet tended to be more tolerant than red seed millet with optimizing photosynthetic capacity through enhancing leaf area, leaf longevity, and enhancing antenna pigments such as chlorophyll b and carotenoid.Keywords: Growth, millet, photosynthetic pigments, shading, yield
UJI TOLERANSI TANAMAN KENTANG HITAM (PLECTRANTHUS ROTUNDIFOLIUS (POIR.) SPRENG.) HASIL RADIASI SINAR GAMMA TERHADAP CEKAMAN KEKERINGAN Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2310

Abstract

Plectranthus rotundifolius (Poir.) Spreng. is one of the potential root crops to become an alternative food. The aim of thisstudy was to determine drought resistant status of Plectranthus rotundifolius irradiated by gamma irradiation. Thisexperiment was conducted in the Green House using a Completely Randomized Design with 2 factors and 5 replications.The first factor was plant number consisted of 7 accessions (D116, D69, M343, D40, M95, D3, and Klefa Imut/KI). Thesecond factor was the level of field capacity (FC) consisted of 100% FC, 60% FC, and 20% FC. The observed parameterwere vegetative and generative growth stage, stomatal conductance, leaf water potential, and chlorophyll content. The resultshowed that the resistance level of the 7 accessions can be devided into 3 categories: 1) The plants that were susceptible todrought i.e. D116, D69, M343, and KI; 2) The plants that were semi-tolerant to drought i.e. D40 and M95; and 3) The plantthat was tolerant to drought i.e. D3.Keywords: Plectranthus rotundifolius, gamma irradiation, drought stress
Co-Authors ,, Witjaksono ,, Witjaksono A. Fitriani A. Saiful Aziz AA Agustia Sinta Dewi AA Sudharmawan, AA Abdul Ghoni, Aris Adekamisti, Revi Adian Fatchur Rochim Afis, Afis Agussetiandari, Indah Agustina, Indah ahmad yani Airlangga, Tri Juda Aisyah, Sabila Aji, Sherly Ratih Frichesyarius Santi Akhmad Nurasikin Aleda Mawene Alfizi, Alfizi Ali Imron Alighiri, Dante Amalia, Sinta Amin Suhadi, Amin Amin Taufiq Amin Taufiq Kurniawan Amin Taufiq Kurniawan Andi Parenrengi Ani Retno Prijanti Anisa Rahmawati Anisa, Winda R Apriani, Suci Saftari Aria Mulyapradana, Aria Arief Kurniawan Arif Rahman Arum, Anggias Ni Pandan Asri Wulan Aulia Oktaviana, Ersa authoria, Nila Awang Maharijaya Ayuningtyas, Roro Aji Azid, Saffanah Binti Mohd Ab Azizah Khakim, Ardha Azizah, Zulfina Nurarinda Azka Adriliya, Putri Azulfa Audira, Regina Bakar, Lastuti Abu Bakhtiyar, Annisa Carolina Batubara, Mutiara Azhar Belangi, Siska Putri Bella Sari, Fianka Bintang Hanggoro Putra Budi Kartiwa Bustamil, Bustamil Chairil Zaman Choiriyah, Nur Fadlilatul Chotimatul Azmi, Chotimatul Corneyla, Novia D Andayani, Retno Dalia Sukmawati Daradjati, Keke Pahlevi Darmawan Saptadi, Darmawan Darmawan, Kevin Deddy Ilyas, Deddy Deden Tarmidi Desyanti Desyanti Desyanti Deviana Yuanitasari Dewi Kania Sugiharti Dewi, Yanuar Kusuma Dian Novitasari Diana Vivanti Sigit Dianita Ekawati Diyah Martanti, Diyah Dodi Sukmayadi Dodiet Aditya Setyawan Dwi Astuti Dwi Retna Sulistyawati Dwiria Wahyuni Edy Cahyono Eka Fatmawati Tihurua Ellysa Ellysa ELVI MAILANI Emma Suryati Endah Sri Hartatik Erfandi, Eli Eri Sofiari ERNI WIDAJANTI Erpurini, Wala Etty Riani Everhard, Everhard Markiano Solissa F. AHMAD Faarist, Rifqi Naufal Fahrur Rozi Fakhruddin Fathur Rohmah, Hajar Nur Fatmawati, Siti Selviyah Fauziah, Chusnul Fauziah, Salma febriyanti, eka Febriyanti, Shintia Fierrelanda, Donna Firdaus, Usamah Putra FITRIYAH, NUR Gellysa Urva Gimin Gimin Handayani, Zidni Ilma Harjono Harjono Harmi, Hendra Hasanah, Isnaeni Helmi Kurniawan Heri Suprapto Hia, Jessica Stephanie Hidayah, Salsabila Nur Hidayat, Moh Taufik Hidayati, Andi Hutadjulu, Ryan Dwitama I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. Ida Nurlinda Ika Nurul Fitriani Ika Roostika Ilhami, Maulidya Indah Yuni Astuti, Indah Indradewa, Rhian Ineu Sulastrini Insih Wilujeng Intan Sari Rufiana Irawadi, Yudi Irawadi, Yudi Irawati, Titik Irsan Irsan Istiqomah, Roshydatul Iteu Margaret Hidayat Jayanti, Esti Jumili Arianto Juniarso Juniarso Karaman, Jamilah Kartika Ning Tyas Kasliono Kasliono Kurniawati, Ade Kusumanegara, Kusumawaty Laksono, Alya Tasya Putri Lastuti Abubakar Leksonowati, Aryani Leksonowati, Aryani Lestiyono, Sidik Liza Azizah M. Hidayat, Iteu M. Syamsul Maarif M.H, Andi Asnur Pranata Maemaunah, Maemaunah Mahfudoh, Siti Mandasari, Febry Mangara Sihaloho Manjani, Nur Hudayah Manurung, Elsa Debora Mardiyanti, Yanti Maria Ulfah Martinus Budiantara, Martinus Marzuki, Muhammad Ervan Masdeniati, Masdeniati Masruri, Ali Maulana Maulana, Maulana Maulana, Haris Maulana, Sincan Merina Pratiwi Moersilah, Moersilah Mohammad Alauhdin, Mohammad Mokay, Meggy Merlin Muhamad Amirulloh, Muhamad Muhammad Ihsan Ramadhan Muhammad Soleh Susanto Muhammad Zilal Hamzah, Muhammad Zilal Mulyana, Denio Artanipa Murdianingsih, Dian Musa Hefer Smas Mustamin, Kaso Nafi’, Zaky Luthfirana Roihan Nani Heryani Nanik Wijayati Nazori Djazuli Netty Ino Ischak Niam, H. Muhammad Alfa Nihayah, Anini Ningsih, Diah Wuriah Ningsih, Sera Noerdiana, Yusep Noorma, Nilam Noornisa, Essa Aurora Novianto, Muhammad Rifqi Novianty, Elva Novita, Indah Nugrahaeni, Dena Ritmi Nugrahaningsih WH, Nugrahaningsih Nugraheni, K. Utami Nugraheni, K. Utami Nuni Widiarti Nur Hanifah Nur Rachmat Nuraisyah, Devi Nurasikin , Akhmad Nurfitria Tsani, Nia Nurhasanah, Tiara Nurhayati Nurhayati Nurliani Bermawie Nurlis, Nurlis Nurul Khumaida Nusmiyati, Nusmiyati Nuur, Muhammad Alfiyan Pahrul, Yolanda Panjaitan, Priscilla Iranata Perangin-angin, Laurensia M. Permana, Dany Permatasari, Bella Poerbantoro, Heri Pramudyani, Lelya Pranata , Andi Asnur Pranata MH, Andi Asnur Pranata, Andi Asnur Prasetya, Victor Prasetyo, Arif Hadi Pratama, Muhammad Ath-Thaariq Pratama, Sisi Hernanda Pratama, Yoga Putra Prawestri, Apriliana Dyah Prayogi, Agung Priyo Wahyudi Puspitasari, Sherly Indah Putra, Gilang Kharisma Putra, Sony Adiya Putri Haryanti, Ari Putri, Nazwa Adiesta Kurnia Putut Suharso Rahayu, Annisa Rahayu, Resa Sri Rahayu, Siti Intan Rahmah, Dian Maris Rahmatika, Widyana Rambe, Najwa Syifa Aina Ratna Komala, Ratna Ravie, Muhammad REFLINUR REFLINUR Relly Andayani REZA FAHLEVI Riastiwi, Indira Riastiwi, Indira Ridwan Ridwan, Ridwan Rika Lestari Rima Aprilia Rini Riastuti, Rini Rini Yuliani Riri Syafitri Lubis Riska Nurtantyo Sarbini RISMA SITOHANG RITA ANDINI Rizhal Hendi Ristanto Rizkiah, Miftahur Rizky Aulia Romadona, Mia Rahma Ronny Suwento, Ronny Rosanti, Aulia Dewi Rosmaini Rosmaini, Rosmaini Rosyadi, Noor Rukiyanto Rukiyanto, Rukiyanto Rusmiati, Elis Rustam Aji, Rustam Rustam, K. Yoswita S. Poerba, Yuyu S.kom, Ardison Sadam, Azriel Viero Safira, Syahnas Safitri, Eka Dwi Sahati, Sahati Saiful Aziz, A Salamiah . Samhudi, Moh. Sapulette, Viona Saputra, Juni Saragih, Bagus Saraswati, Kunti Dewi SARI, MAYA PUSPITA Sari, Rini Arvika Sarwoedi Satriya Wibawa, Satriya Wibawa Sa’adiyah, Halimah Sellyana, Ari Setiyo Wibowo, widodo Setyo Utomo, Pamuji Sholihin, Achmad Singgih Tri Sulistiyono Siti Hasanah Sobir Sobir Sobiyanto, Ahmad Sopamena, Ronald Fadly Sormin, Feber Sri Mawarni, Sri SRI RAHAYU Sri Tutur Martaningsih Sri Wulandari Suci Rahayu Suciningtyas, Santi Sugiati, Melyi Suhaeti, Eti Sukamto Sukamto Sulardi . SULISTIYANI SULISTIYANI Supartono - Supiyani, Atin Supriatin, Dyah Susi Herawati Sutarto Sutarto Syamsul Bahri Tanudiharja, Gabriella Fransisca Tarwa Mustopa Taufiqurrohman, Ofik Teguh Tresna Puja Asmara Titik Susilowati Tri Yuliati Tugino Ubbadul Adzkiya’ ulummudin, ihya Ulumuddin, Imam Khoirul UTUT WIDYASTUTI W, Supraba Sekarwati Wahyu S, Riswan Eko Wajnah Waluyo, Nurmalita Wasito Wasito Wawan Trisnadi Putra, Wawan Trisnadi Welly Desriyati Wenti Ayu Sunarjo Widayanti, Firda Mutia Widianingsih, Euis Widodo, Putranto Hari Witjaksono Witjaksono Witjaksono Wowo Afif Fathurohman, Wowo Afif Wulan Sari, Nunung Y. Djoko Suseno Yety Rochwulaningsih Yohanes Purwanto Yon Sugiarto Yudiwanti Wahyu E. Kusumo Yuli Christyono Yuli Rohmiati Yulia Pratiwi, Riska Yuliati, Tri Yunita, Linda Desma Yunita, Linda Desma Yunitaningrum, Utari Yusma Yennie, Yusma Yusrina, Yusrina Ruzana Yuyu S Poerba Yuyu S. Poerba, Yuyu S. Yuyu Suryasari Poerba Yuzammi Yuzammi Zaky, Adrian Maulana Zizlavsky, Semiramis