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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Buletin Hama dan Penyakit Tumbuhan INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Masalah-Masalah Hukum LAW REFORM Zuriat Jurnal Natur Indonesia Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Microbiology Indonesia Journal of Tropical Soils DE JURE Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati ELT Worldwide: Journal of English Language Teaching Jurnal Tematik Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar Pelita Perkebunan Wacana, Jurnal Sosial dan Humaniora Jurnal RISET Geologi dan Pertambangan AGRIVITA, Journal of Agricultural Science Brawijaya Law Journal : Journal of Legal Studies Jurnal NERS Jurnal Agroekoteknologi International Journal of Sustainable Tropical Agricultural Sciences Indonesian Journal of Biotechnology JFA (Jurnal Fisika dan Aplikasinya) Techno LPPM Jurnal Media Hukum Planta Tropika Enthalpy : Jurnal Ilmiah Mahasiswa Teknik Mesin UNEJ e-Proceeding Jurnal Penelitian Karet Jurnal Ilmiah Pendidikan Pancasila dan Kewarganegaraan JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Ilmu Nutrisi dan Teknologi Pakan ANNALES BOGORIENSES Buletin Sumber Daya Geologi Constitutional Review Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA ICoTE Proceedings Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Bioma : Jurnal Biologi Indonesia JCES (Journal of Character Education Society) Mimbar Yustitia : Jurnal Hukum dan Hak Asasi Manusia JSKK (Jurnal Sains Keolahragaan dan Kesehatan) Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science Gorontalo Management Research JOURNAL OF SCIENCE AND SOCIAL RESEARCH EDUPEDIA SIGMA Jurnal Ilmiah Mandala Education (JIME) JISIP: Jurnal Ilmu Sosial dan Pendidikan JUPE : Jurnal Pendidikan Mandala Menara Perkebunan Jurnal Teknologi Terapan Widya Balina :Jurnal Ilmu Pendidikan dan Ekonomi Berkala Penelitian Agronomi Jurnal Review Pendidikan dan Pengajaran (JRPP) JPP Jurnal Kesehatan Poltekkes Palembang JURNAL PENDIDIKAN MIPA Jurnal Pengabdian Kepada Masyarakat (Mediteg) RADIAL: JuRnal PerADaban SaIns RekAyasan dan TeknoLogi Journal of English Language Teaching Innovations and Materials (Jeltim) Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Jurnal Ilmiah Edunomika (JIE) SIGn Jurnal Hukum Jurnal Prima Medika Sains Proceedings of Annual Conference for Muslim Scholars Jurnal Abdi Insani JIIP (Jurnal Ilmiah Ilmu Pendidikan) Jurnal Tanah dan Iklim Jurnal Agrotropika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Indonesia Berdaya SUPERMAT (JURNAL PENDIDIKAN MATEMATIKA) Jurnal Promotif Preventif Yumary: Jurnal Pengabdian kepada Masyarakat Jurnal Cahaya Mandalika Nusantara Journal of Islamic Studies Bima Abdi: Jurnal Pengabdian Masyarakat JagoMIPA: Jurnal Pendidikan Matematika dan IPA Jurnal Penkomi : Kajian Pendidikan dan Ekonomi Jurnal Inovasi Pembelajaran di Sekolah Journal of Education Research Jurnal Pengabdian Masyarakat Ilmu Terapan (JPMIT) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Pengabdian Ilung (Inovasi Lahan Basah Unggul) International Journal of Learning and Instruction (IJLI) Jurnal Ilmu Hukum, Humaniora dan Politik (JIHHP) Zona Kedokteran: Program Studi Pendidikan Dokter Universitas Batam Bacarita Law Journal Trunojoyo Law Review Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia Journal of Scientific Research, Education, and Technology IDARAH : Jurnal Pendidikan dan Kependidikan Jurnal Inovasi Pengabdian dan Pemberdayaan Masyarakat Jurnal Keperawatan dan Kesehatan (JKK) TELL - US JOURNAL Sewagati Perbal: Jurnal Pertanian Berkelanjutan West Science Journal Economic and Entrepreneurship Jurnal Pelita Manajemen Journal of General Procedural Dermatology and Venereology Indonesia Jurnal Edukasi Biologi Kingdom (The Journal of Biological Studies) Journal of Islamic Education and Ethics JOEEL (Journal of English Education and Literature) Jurnal Pengabdian Kepada Masyarakat Darma Saskara Jurnal Pendekar Nusantara Jurnal Penelitian Pendidikan Indonesia Journal of Law and Legal Reform Jurnal Ap-Kain Jurnal Ekonomi, Bisnis dan Sosial Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) Lire Journal (Journal of Linguistics and Literature) Jurnal Faidatuna Jurnal Kajian Akuntansi, Auditing dan Perpajakan Jurnal Natur Indonesia Jurnal Legislasi Indonesia National Journal of Law English Education: English Journal for Teaching and Learning Jurnal Penginderaan Jauh dan Pengolahan Data Citra Digital RADIAL: Jurnal Peradaban Sains, Rekayasa dan Teknologi Annales Bogorienses

Sudarsono

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jalan Meranti Kampus IPB Dramaga, Bogor 16680

Author-ID : 387783

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Pengaruh Komposisi Media terhadap Pembentukan Kalus Embriogenesis Somatik Kopi Arabika ( Coffea arabica ) Ibrahim, Meynarti Sari dewi; Sudarsono, Sudarsono; Rubiyo, Rubiyo; Syafaruddin, Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 3, No 1 (2012): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Induksi embrio somatik pada kopi arabika (Coffea arabica) dengan menggunakan beberapa zat pengatur tumbuh (ZPT) telah berhasil dilakukan. Pengaruh komposisi media terutama kombinasi antara jenis ZPT yang berbeda dan tanggap genotipe tanaman dilaporkan sangat bervariasi. Tujuan penelitian untuk mengkaji pengaruh pemberian 2,4-D dan kinetin dalam proses pembentukan dan pertumbuhan kalus embriogenik asal daun. Penelitian dilakukan di Laboratorium Pusat Penelitian dan Pengembangan Perkebunan (Puslitbangbun) Agustus 2011 sampai Januari 2012. Bahan tanaman yang digunakan adalah daun dari kopi arabika varietas Sigarar Utang yang merupakan tanaman koleksi Balai Penelitian Tanaman Industri dan Penyegar (Balittri). Penelitian menggunakan rancangan acak lengkap dengan 5 ulangan, masing-masing ulangan terdiri dari 5 eksplan. Peubah yang diamati, meliputi persentasi kalus yang terbentuk, morfologi kalus, berat basah kalus, dan jumlah globular. Hasil menunjukkan semua perlakuan dapat membentuk kalus, pertambahan berat eksplan tertinggi diperoleh pada media kombinasi 2,4-D 1 mg/l atau 2 mg/l dan kinetin 1 sampai 4 mg/l. Embrio somatik terbanyak diperoleh pada media yang diberi 2,4-D 0,5 mg/l dan kinetin 1 mg/l. Selain kalus, massa proembrio dan embrio, juga terbentuk akar adventif yang jumlahnya tidak nyata antar perlakuan.Â The Effect of Composition Media to Callus Formation of Somaticembryogenesis of Arabica Coffee (Coffea arabica)ABSTRACT Induction of somatic embryos with plant growth regulators (PGR) has successfully performed in arabica coffee. However, the influence of media composition combined with different PGR, explants and genotype of plants is widely various in response yields. The objective of this study was to examine the effects of 2,4-D and kinetine in process of formation and growth of embryogenic callus developed from leaves of arabica coffee. The studiy was carried out at a laboratory of Indonesian Research Center for Estate Crops (Puslitbangbun) from August 2011 to January 2012. Plant materials used are coffee leaves var. Sigarar Utang taken from a germplasm collection of the crop grown at Pakuwon Research Station, Indonesian Research Institute for Industry Crops (Balittri) located at Sukabumi, West Java. A completely randomized design with 5 replications and plot size of five explants was used. Parameters observed are percentage of callus formation, morphology of the callus, fresh weight of callus, and number of globular. Results show that all treatments examined are able to form callus. The highest increase in weight of explants was obtained from the media treated with 2,4-D (conc. of 1mg/l or 2 mg/l) and kinetin (conc. of 1 to 4 mg/l). While, the most number of somatic embryo formed was obtained from those of treated with 2,4-D 0.5 mg/l and kinetin 1 mg/l. In addition to callus formation, proembryo mass, embryo and adventive roots were also formed in spite of not significant between different the treatments.

Isolation and Analysis of DNA Fragment of Genes Related to Kopyor Trait in Coconut Plant S, Sukendah; Volkaert, Hugo; S, Sudarsono
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

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Kopyor coconut is a natural mutant that has abnormal endosperm development. For the first time several genes that were suspected to be related to kopyor trait were identified based on the chemical compounds of the endosperm that different from that of normal coconut. Sucrose synthase (SUS), Stearoyl acyl carrier protein desaturase (SACPD), and Absicid acid insensitive (ABI) genes were isolated and analyzed. Four DNA fragments with length of 746, 738, 780, and 687 bp (CnSus1A, CnSus1B, CnSus2A, and CnSus2B) were obtained from SUS gene. Sequence analysis at DNA and amino acid level showed that CnSus1A, CnSus1B, CnSus2A, and CnSus2B were classified into monocot SUS group with nongrass SUS type. Isolation of SACPD gene resulted in one DNA fragment with DNA length of 716 bp. CnSacpd shared a high homology with SACPD gene of oil palm and soybean. Isolation of ABI gene resulted in two DNA fragments, CnAbi3A and CnAbi3B, with DNA length of 760 and 728 bp, respectively. CnAbi3A and CnAbi3B showed a high homology with ABI3 gene of several plants. All DNA fragment obtained from SUS, SACPD, ABI genes were used as templates to design spesific markers for each corresponding gene. There were 7 specific primer sets designed, i.e., CnSUS1A, CnSUS1B, CnSUS2A, CnSUS2B, CnSACPD, CnABI3A, and CnABI3B.<

The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment Avivi, Sholeh; Dietzgen, Ralf G.; Higgins, Colleen M.; ., Sudarsono
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The aims of this research were to (1) compare the effectiveness of established Agro bacterium mediated transformation and bombardment mediated transformation on peanut (2) evaluate the integration of green fluorescent protein (GFP) and hygromycin resistance (hygr) gene on the peanut embryosomatics. To achieve those objectives we shoot 1200 explants using bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both of Agrobacterium mediated transformation and particle bombardment could give positive embryos transformed.Â Particle bombardment gave 4.5% transformation efficiency while Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that particle bombardment more efficient and gave more transgenic explants than Agrobacterium mediated transformation. Keywords: agrobacterium, bombardment, GFP.

IDENTIFIKASI KETAHANAN PLASMA NUTFAH KARET IRRDB 1981 TERPILIH TERHADAP PENYAKIT GUGUR DAUN CORYNESPORA BERDASARKAN AKTIVITAS TOKSIN CASSICOLIN Oktavia, Fetrina; Sudarsono, Sudarsono; Kuswanhadi, Kuswanhadi; Dinarty, Dini; Widodo, Widodo
Jurnal Penelitian Karet JPK : Volume 34, Nomor 1, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i1.225

Salah satu penyakit pada tanaman karet adalah penyakit gugur daun Corynespora (PGDC) yang disebabkan oleh jamur Corynespora cassiicola. Patogen tersebut dapat menyerang semua tahap pertumbuhan tanaman karet yang menyebabkan terjadinya penurunan hasil lateks yang cukup signifikan dan bahkan dapat menyebabkan kematian tanaman. Penggunaan klon-klon tahan sebagai bahan tanam merupakan cara yang paling efektif dan ekonomis untuk mencegah terjadinya serangan PGDC. Karena itu identifikasi klon-klon resisten merupakan strategi utama dalam manajemen PGDC. Penelitian ini bertujuan untuk mengidentifikasi tingkat ketahanan 50 genotipe terpilih plasma nutfah IRRDB 1981 (PNâ81) terhadap PGDC. Enam klon karet yang berasal dari populasi Wickham digunakan sebagai pembanding (BPM 24, BPM 1, GT 1, RRIC 600, PB 260 dan RRIM 600). Empat isolat C. cassiicola (CC-01, CC-20, CC-22, dan CC-23) diinokulasikan masing-masing pada daun muda tahap pertumbuhan B2C, dan intensitas kelayuan daun dihitung berdasarkan estimasi kehilangan air akibat aktifitas patogenisitas toksin. Hasil penelitian menunjukkan bahwa 12 genotipe tergolong sangat tahan, 13 genotipe tergolong tahan, 23 genotipe tergolong rentan dan 8 genotipe tergolong sangat rentan. Genotipe PN 451, PN 494 dan PN 604 menunjukkan tingkat ketahanan yang lebih baik terhadap PGDC dibandingkan dengan populasi Wickham sehingga ketiga genotipe tersebut berpotensi digunakan sebagai sumber gen ketahanan dalam program pemuliaan tanaman karet.Â Diterima : 13 Maret 2016 / Direvisi : 29 Juli 2016 / Disetujui : 2 Agustus 2016Â How to Cite : Oktavia, F., Sudarsono, S., Kuswanhadi, K., Dinarty, D., & Widodo, W. (2016). Identifikasi ketahanan plasma nutfah karet IRRDB 1981 terpilih terhadap penyakit gugur daun Corynespora berdasarkan aktivitas toksin Cassicolin. Jurnal Penelitian Karet, 34(1), 35-48. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/225

Isolation and Characterization of OsNAC6 cDNA from Rice (Oryza sativa L.) cv. Nipponbare, Batutegi, and Rojolele Rachmat, Agus; Nugroho, Satya; Nurdiani, Dini; Swastika, Maria; Sukma, Dewi; Aswidinnoor, Hajrial; Sudarsono, Sudarsono
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Transcription factors have an important function in regulating gene expression and plant responses to stresses. The ERF, bZIP, WRKY, MYB, and NAC are stress inducible transcription factors. The OsNAC6 is a member of the NAC transcription factor family in rice and its expression is induced by abiotic stresses, wounding and blast disease. Characterization of OsNAC6 gene sequences would give a better undersanding on how OsNAC gene functions biologically. The objectives of this research are to isolate the OsNAC6 cDNA from Nipponbare, Batutegi, and Rojolele cultivars, to characterize their DNA sequences, and to compare their sequences to other NAC genes from other plants available in GenBank DNA databases. Isolated cDNA and sequencing of the fragments resulted in a 912 bp DNA sequences. Translation of the sequences yielded a protein consisted of 303 amino acid residue. Blast analysis of amino acid sequences indicated identity of isolated cDNA from three Indonesian rice cultivars are the OsNAC6 gene. Deduced amino acid residues from amplified cDNAs of Nipponbare, Batutegi, and Rojolele cultivars shared 100% sequence identities to rice OsNAC6 (Acc. # BAA89800), 71-100% sequence identity to a number of OsNAC protein from Oryza sativa and 63-83% sequence identity to NAC protein from other plants.

ANALISIS BIOINFORMATIKA BERBASIS WEB PADA SEKUEN GENOM PARSIAL SAGU (Metroxylon sagu Rottb.) Purwoko, Devit; Cartealy, Imam Civi; Tajuddin, Teuku; Dinarti, Diny; Sudarsono, Sudarsono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

WEB-based bioinformatic analysis on partial genome sequence of Sago (Metroxylon sagu Rottb.)ABSTRACTSago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequences were analyzed using Blast2Go to determine the genetic function annotation, putative gene identification was performed on the Arabidopsis database using the BLASTx program with a 10-3 e-value limit on The Arabidopsis Information Resource (TAIR) (http://www.arabidopsis.org/index.jsp). Gene interactions were analyzed using DAVID and GeneMania programs. Based on sequence analysis with Blast2Go, 33 sequences with Blastx hit consisting of: 29 sequences had a high homology. The sago sequences with a similarity of ≥ 90% are glutamate decarboxylase and HT1-like serine threonine kinase with hit number 10. The distribution of interactions between genes from GeneMania analysis is known to be mostly interconnected in the 65.13% protein domain, predicted 19.83%, genes with 14.47% shared expression and the remaining 0.57% had localization together.Keywords: bioinformatics, gene annotation, gene ontology, genome sequence, Metroxylon sagu ABSTRAKKajian analisis sekuen genom sagu hingga saat ini masih amat terbatas. Penelitian ini merupakan riset pendahuluan analisis sekuen sagu yang diperoleh dari teknologi NGS untuk mengetahui dan mengidentifikasi sekuen gen baru yang memiliki homologi dengan gen pada database NCBI. Sekuen dianalisis menggunakan perangkat Blast2Go untuk mengetahui anotasi fungsional gen, identifikasi gen putatif dilakukan terhadap database Arabidopsis menggunakan program BLASTx dengan batas e-value 10-3 pada The Arabidopsis Information Resource (TAIR). Interaksi gen dianalisis menggunakan program DAVID dan GeneMania. Berdasarkan analisis sekuen dengan Blast2Go, diperoleh 33 sekuen dengan Blastx hit yang terdiri atas: 29 sekuen memiliki homologi yang tinggi. Gen dengan rataan kemiripan ≥ 90% adalah glutamate decarboxylase dan serine threonine-kinase HT1-like dengan jumlah hit 10. Persebaran interaksi antar gen hasil analisis GeneMania diketahui sebagian besar saling terkait pada domain protein 65,13%, koneksi yang berhasil diprediksi 19,83%, gen dengan ekspresi bersama 14,47% dan sisanya 0,57% memiliki peranan bersama. Kata Kunci: anotasi gen, bioinformatika, Metroxylon sagu, ontologi gen, sekuen genome

The Induction of Primary and Secondary Somatic Embryo to Support Arabica Coffee Propagation Ibrahim, Meynarti Sari Dewi; Hartati, Raden Roro Sri; Rubiyo, Rubiyo; Purwito, Agus; Sudarsono, Sudarsono
Journal of Tropical Crop Science Vol 2 No 3 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

The primary and secondary somatic embryogenesis can be used to propagate Coffea arabica L clonally. However, the success of this propagation was depended on plant growth regulator and varieties. This study aimed to examine the possibility of 2,4-D and thidiazuron application to form primary and secondary somatic embryo to support Arabica coffee clonal propagation. The study consisted of two activities (1) 2,4-D and thidiazuron Application to Induce Primary Somatic Embryogenesis of Arabica Coffee and (2) The Application of thidiazuron in Solid and Semi-Solid Media to Induce Secondary Somatic Embryos. The results indicated significant effect of varieties and plant growth regulator on fresh weight, number of torpedo and germinated embryo. However, it showed no significant effect on callus formation percentage. The best medium to induce primary somatic embryogenesis depending on variety, on the treatment of 4.52 μM 2,4 -D +18.16 μM thidiazuron was the best for AS2K and Sigarar Utang varieties, S 795 at 4.52 μM 2,4-D + 9.08 μM thidiazuron, whereas Kartika at 4.52 μM 2.4-D + 13.62 μM thidiazuron. The morphology of coffee somatic embryo was normal. Primary somatic embryo was developed indirectly, whereas the secondary somatic embryo was directly. The application of 9.08 μM thidiazuron increased the percentage and number of secondary somatic embryos, hence enhancing number of Arabica coffee planlet. Keywords : Coffea arabica L, 2,4-D, thidiazuron, semi-solid media, Indirect somatic embryogenesis

Resistensi Kacang Tanah Transgenik yang Membawa Gen cp PStV Stabil Sampai Tujuh Generasi Silang-Dalam terhadap Peanut Stripe Virus Hapsoro, Dwi; Aswidinnoor, Hajrial; Jumanto, Jumanto; Suseno, Rusmilah; Sudarsono, Sudarsono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Penyakit bilur kacang tanah yang disebabkan oleh peanut stripe virus (PStV)merupakan salah satu penyakit utama pada pertanaman kacang tanah (Arachishypogaea L.). Penggunaan varietas tahan merupakan alternatif paling efektif untukmengatasi penyakit tersebut. Rekayasa genetika merupakan metode efektif untukmendapatkan varietas kacang tanah yang resisten PStV. Tujuan penelitian ini adalahuntuk (1) mengetahui respons tanaman kacang tanah transgenik yang membawa gen cpPStV terhadap infeksi PStV dan (2) menguji stabilitas transgen sampai tujuh generasisilang-dalam. Tanaman kacang tanah transgenik cv. Gajah generasi T0, T1, T2, T3, T5,T6, dan T7 diinokulasi secara mekanik dengan PStV. Terdapat tiga jenis respons kacangtanah transgenik terhadap infeksi PStV, yaitu resisten, recovery, dan rentan. Padatanaman resisten gejala tidak muncul. Pada tanaman recovery, gejala chlorotic ringmottle muncul pada satu daun atau lebih, selanjutnya gejala tidak tampak pada daundaunyang tumbuh kemudian. Pada tanaman rentan, gejala severe blotch muncul padasuatu daun, selanjutnya gejala tersebut tetap muncul pada seluruh daun yang tumbuhkemudian. Transgen cp PStV tetap stabil setelah mengalami tujuh generasi silangdalam.Sejumlah galur murni kacang tanah transgenik yang resisten PStV telahdiidentifikasi.

Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci Raynalta, Erick; Elina, Juanita; Sudarsono, Sudarsono; Sukma, Dewi
AGRIVITA, Journal of Agricultural Science Vol 40, No 3 (2018): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i3.1493

Phalaenopsis amabilis is an Indonesia native orchid species having large, white flowers with yellow labellum coloration. This studies aimed to develop Phal. amabilis micropropagation methods and evaluate the regenerated plantlet fidelity. Media supplemented with Thidiazuron (TDZ) and Polyvinylpyrrolidone (PVP) and medium pH adjustment effects to induce protocorm-like bodies (PLBs) from leaf explants and proliferate secondary PLBs were investigated. Clonal fidelity among regenerated plantlets was evaluated using eighteen SNAP marker loci. The results showed that the Â½ MS medium supplemented with 3 mg L-1 TDZ and 0.5 g L-1 PVP was the best for PLB induction while the Â½ MS medium supplemented with 0.5 mg L-1 TDZ was the best for PLB proliferation. For PLB induction, the media pH was adjusted into pH=7 for efficient PLB regeneration. Based on the assessment using 18 SNAP marker loci, four variant alleles in three loci (11.8%) out of a total 34 plantlets were detected. The mutation frequency at the evaluated SNAP marker loci was 2.5 x 103 (0.25%). Changes in SNP alleles may not always result in phenotype changes and allele variant occurrences may not affect phenotype fidelity of micro-propagated Phal. amabilis plantlets. Therefore, further studies about the phenotype fidelity among plantlets are necessary.

Pewarisan Sifat Densitas Stomata dan Laju Kehilangan Air Daun (rate leaf water loss RWL) pada Kacang Tanah (Arachis hypogaea L.) Adisyahputra, Adisyahputra; Sudarsono, Sudarsono; Setiawan, Kukuh
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

The aim of this research is to analyze and examine the inheritance of stomatal density trait and RWL as a variable in drought tolerance ofpeanut. The experiment was conducted by using cv. Kelinci that is sensitive genotype as female parent and US 605 which is tolerantgenotype as male parent, including population off spring from hybrid cv. Kelinci (P1) with US 605 (P2). Stomatal density was determinedby making leaf imprint and by observing leaf imprint under microscope. Relative water loss was determined by dipping peanut leaf in PEG40% for 48 hours. Result of the analysis showed that stomatal density and RWL were not only controlled by qualitative characters of majorgene, but also controlled by quantitative character of minor gene by polygenic with the complex gene action. Both characters seem toinfluence more as genetic factor and have high level fixation additive varians which can give the opportunity to obtain the tolerant offspring.

Co-Authors , Agustiansyah , Rustikawati , Sukendah , Triadiati , Witjaksono . DARMAWAN . Giyanto . Iskandar . Kurniati . Suwardi A. Farid .Hemon Abad, Hulud Abd. Kadir Abdul Madjid ABDUL RACHMAD BUDIONO Achwan, Muhammad Ade Mariyam Oklima Ade Mariyam Oklima Ade Wachjar Ade Wachjar Adisyahputra Adisyahputra, Adisyahputra Afandi, Muhammad Yusron Afifulloh, Afifulloh Agung Endro Nugroho Agung Wahyu Susilo Agus Purwito Agus Rachmat Agustini, Rika Yayu Ahmad Fathoni Ahmad Musaddad, Ahmad AHMAD RIDUAN Ahmad Surkati Ahmad, Evi Aksar, Prinob Alfian, Hamzah Ali Husni Amalia, Fadila Amarullah, Muhamad Roffi Ambarwati, Sinta Devi Amelia, Septiyan Nur Aminur Aminur Aminuyati Ananta, Puja Asti Andeas, Andeas Andrie Al Kausar Anita Yuliyanti Aniversari Apriana Anjarsari, Nurul Aeni Anneke Pesik Ansyori, Ansyori Anugrah, Ridho Anwar, Aswaldi Apriliani, Elisa Aprizal Zainal Arfan Nazhri Simamora Arief Hidayat Arif, Mochamad Syaiful arifin tahir Aris Munandar Ariswan, Ariswam Arrosyadi, Muhammad Ilham ASMONO, DWI Aspiana, Endah Asri, Hendri Rahmayani Atang Sutandi Atmitri Sisharmini Ayu Amelia Azhar Nuh, Fuad Bachtera Indarto, Bachtera Bachtiar, Iyas Muzalif Badrut Tamam Bahar Bahar, Bahar Bambang Heliyanto Bambang S. Purwoko BAMBANG SUGIRI Barahima Abbas Bernadetta Octavia Bonadi, Ahmad Budi Santoso, Slamet Budiman Sudia, Budiman Budiwati, Budiwati - C J Soegihardjo Cahyaningrum, Lulik Tri Cahyono, Andy Agus Cartealy, Imam Civi Cartealy, Imam Civi Cecep Kusmana Chairul, Muhammad Christina, Yani Christoph Leuschner Chusni Ansori Clarry Sada Colleen M. Higgins, Colleen M. Darmawan Saptadi Darmawan, Rizqi Wahyu Deka Rotama Desi Pujiati, Desi Desta Wirnas DEWI SARTIKA Dewi Sukma Dhanu, Rusli Didiek Hadjar Goenadi Diky Anggoro Dina Lusianti Dinarti, D DINARTY, DINY Dini Dinarty, Dini DINI NURDIANI Diny Dinarti Djoko Santoso DWI ANDREAS SANTOSA Dwi Asmono DWI HAPSORO Dwi Hapsoro Dwi Riyanti Dwiputra, Adnan Wahyu Dyah Ochtorina Susanti Dyah Tjahyandari Suryaningtyas Edi Guhardja Edwinarta, Caesar Dimas Efendi, A'an Efendi, Aan Efendi, Darda Eka Fajar Rahmani Ekastuti, Zaidatun Elina, Juanita ELSJE T. TENDA, ELSJE T. Endang Pudjihartati Endang Susilawati Endrizal Eneng Fakhrunnisa Enni Suwarsi Rahayu Enung Sri Mulyaningsih Erick Raynalta, Erick Ernata, Yusvidha Eusabinus Bunau Fadli, La Ode Muammar fadoli, hendi imam Fahlefi, Reza Fahmi Astuti, Fahmi Fajar Laksono Suroso Fajri, Adz Dzaa Riaat Fakhrunnisa, Eneng Fatimah, Iim Fatmawati, Nurul Fatsan, Ahmad FETRINA OKTAVIA Fitri Yanti Fredyarini Soedjiwo, Novena Ade Gatot Sutapa Yuliana, Yohanes Gatut Yudoyono Germini Alam Ginting, Tarianna Giyanto . Gontjang Prajitno Grantaka, Akbar Maulana Gusti Ayu Kade Sutariati H Yulipriyanto, H H. M.H. Bintoro Djoefrie HAJRIAL ASWIDINNOOR Hari Pamungkas Hari Sutrisno Harsini ,, Harsini Hartanti, Nenik Diah Hartati, Raden Roro Sri Haryadi Permana Hasanat Uzzahroh, Umi HASIBUAN, HOIRUDDIN Hasriadi Mat Akin Heltty Heltty Heltty, Heltty Hendriyanto, Dennyca Hengky Novarianto Hengky Novarianto Herdhata Agusta Herison, Catur Hernandi, Iman Budi Herwantono, Herwantono Hidayat Pawitan Hiroshi Ehara Hugo Volkaert, Hugo I MADE ARTIKA I Nyoman Nurjaya IBNUL QAYIM IDA HANARIDA SOMANTRI Iflaha, Nurul Iim Fatimah Ika Mariska Ikhsanudin Ikhsanudin Ikhsanudin Ikhsanudin Ikhsanudin Imam Mashudi, Imam Imran, Al Ichlas Indriyati, Lilik Tri Insani, Andi Nur Ipaljri Saputra, Andi Ipaljri, Andi Irawan, Dedy Irfan, Aprian Irman Firmansyah Iskandar Iskandar Ismail Maskromo Ismail Maskromo Ita Nuryana Iwa Sutardjo, Iwa Iwan Permadi Iwan Sasli Iwan Setiawan Izroiel, Rabbenstain JAJAH KOSWARA Jaka Seru Dwi Saputra Jaswadi JASWADI, Jaswadi Juang Gema Kartika Juita, Nirmala Jumanto Jumanto Jumanto Jumanto, Jumanto Jumari Jumari, Jumari Jusman Jusman Kadir, Abd . Khaerunnisa, Aisyah Khoerul Anwar Khursatul Munibah Kukuh Murtilaksono Kukuh Setiawan Kurniasari, Praharisti Kurniawan R. Trijatmiko Kurniawan, Choirul Kusumo Nugroho Kuswanhadi Kuswanhadi, Kuswanhadi La Ode Ahmad Barata Laksmita Prima Santi Latifa, Annisa LATIFAH KOSIM DARUSMAN Lila Yuwana Lila Yuwana Lisa Virdinarti Putra Lollie Agustina P. Putri Lubis, Fatma Hani Luwandi Suhartono M. Herman Maera Zasari Mahrusun, Mahrusun Mamik Indaryani Manalu, Victor Manotar Pademan Marbelisa Briliani Mardikaningsih, Agusti Marenda Ishak S Maria Yosepin Endah Listyowati Mariam Mariam MASKROMO, ISMAIL Masluki, Masluki Mawaddah, Ida Maya Megayani Sri Rahayu MEITY A. TULALO, MEITY A. Memen Surahman Merry Sintia Dewi Meynarti Sari Dewi Ibrahim Miguna, Sarita Misar, Misar Moh. Zaini Muadin, Akhmad Muchammad Ali Safaat Muchlisa M, Dewi Ratna Muchtar, Asmaji Muhammad Arifin MUHAMMAD HASBI Muhammad Hasjim Bintoro Djoefrie Hasjim Bintoro Djoefrie Muhammad Hasyim Bintoro Muhammad Herman MUHAMMAD HERMAN Muhammad Ikhsan Muhammad Kamal Muhammad Machmud Muhammad Syukur Muhammad, Ikram Muhson, Akhmad Mulat, Astuti Musfira, Musfira Musthafa, Annas Rasid Mutia Dewi Yuniati N. Sri Hartati, N. Sri Nanang Endriatno Napitupulu, Tosanov T.P. Narindra, Hawin Neviana Wahyuni Nihayati, Hanik Endang Niniet Indah Arvitrida Nooryanto, Fista Herry NOVARIANTO, HENGKY Nugraheni, Titi Nuh, Fuad Azhar Nur Khayati Nur Wahyudi Nur, Rahmat Nurhajati A. Mattjik Nurmalitasari, Melinda Nurmaya, Alya Nursalam, Nursalam Nurul Khumaida Oktafia, Yeni Omaidi, Omaidi Paat, Johny Christofel Pakpahan, Benhur Pane, Putri Yunita Paulus B.K. Santoso Prihatin, Lilik Prija Djatmika, Prija Prita Sari Dewi Purwaningsih - Purwoko, Agus purwoko, devit Purwoko, Devit Puspitasari, Nurrisma Putra, Adhitya Pratama Putri, Halida Adistya Qodarsih, Inna Anzalina Qosim, Achmad R B Gobel Rachman, Azis Rahmadhani, Tasya Rahman, Wahbi Rahmawati, Rika Sri Rajamanickam, Ramalinggam Ralf G. Dietzgen, Ralf G. Ratnawati Ratnawati Razak, Askari Regina Regina Rehulina Rehulina, Rehulina Renaldi, Muhammad Rizal Reni Indrayanti Rina Wati Rita Lefrida Roedhy Poerwanto Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rubbi Kurniawan Rubiyo Rubiyo RULLY DYAH PURWATI Rusmayani, Rusmayani RUSMILAH SUSENO Rustan Rykson Situmorang S. Arsyad S. Ilyas Saefudin Saefudin Sahrul, Sahrul Said Harran Saiful Akhyar Lubis Samhuddin Samhuddin, Samhuddin Samidun, Samidun Sandra, Yulia Sari, Nunuk Tujung Sarif, La Sarudin, Sarudin Satriyas Ilyas SATRIYAS ILYAS Satya Nugroho Septanti, Annisya Setiawan, Asep S Setiawan, Rizki Nur Setiowati, Retno Diah Setyaningrum, Diyah Setyarini, Ekaning Setyawan, Anton Afries Setyawan, Fadjar Ramdhani Setyawan, Trinovandhi Shaleha, Niswatun Shandra Amarillis Shinta Hadiyantina Sholeh Avivi Sidiki, Mey Wulansari Silaen, Jonni Haonangan Sintho Wahyuning Ardie Siregar, Vicentius P. Sisworo, Widjang Herry Siti Mufidah siti nurlela Siti Sunarintyas SOEKISMAN TJITROSEMITO Sri H. Hidayat Sri H. Hidayat Sri H. Hidayat Sri Hastutiningrum Sri Indarto Sri S. Harjadi Sri Soenarsih DAS Sri Wening Stephanie Wilamarta Sudari, Sudari Sudibyo Martono Sudirman Yahya Sudirman Yahya SUDJINDRO SUDJINDRO Sudradjat , Sughiandi, Rachmad Sugianto, Pingkan Sugiri, Bambang Sugiyanta Suhandoyo Suhartini Suhartini Suhermanto, Totok Suhirman, Suhirman Sukendah S, Sukendah SUKENDAH SUKENDAH, SUKENDAH Sumali Wiryowidagdo Sumarni Sumarni Surmiyati Surmiyati Suryo Wiyono Susilo Indrawati Susilo Indrawati Sutji Rochaminah Suwargana , Nana Suwarto, Visia Assyafira Suweni Muntini, Melania Suyatno Suyatno Swastika, Maria Swastika, Maria Syafa'at, Rachmad Syafaruddin Syafaruddin Syafi`ullah, Alfian Syarif Husin Tamaulina Br Sembiring Tambunan, Brio Gideon Tarigan, Sabarita Taufik, Beatrice Ellen TENDA, ELSJE T. Tengku Riza Zarzani N Teuku Tajuddin TINCHE, nFn Tri J. Santoso Tri J. Santoso Tri Joko Santoso Tri Joko Santoso Tri Rini Nuringtyas Tyaningrum, Annisa Mayang U.S WIRADISASTRA Ucu Sumirat UJANG KHAIRUL Ulfa, Umi Tahniah urai salam Utomo, Abet Yudho Uzzahroh, Hasanat vyta andri setyo utami Wahdaniyah, Gusti Wahyu Wahyu Wajdi, Muhammad Warsiyah Warsiyah, Warsiyah Wening Enggarini Widiatmaka Widodo Widodo Wipsar Sunu Brams Dwandaru Wisilayasa, I Gede Darma Wiwik Kusmawati, Wiwik Wiwin Ambarwulan Yadi Setiadi Yanti Sri Rejeki Yobel, Christian Yudiwanti Wahyu E. Kusumo Yuliati - Yulis Setiya Dewi Yundari, Yundari Yuni Wibowo Yusnita Yusnita Yustinus Yustinus, Yustinus Yusuf, Rinto Zahalim Zahalim Zahalim, Zahalim

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