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ANALISIS BIOINFORMATIKA BERBASIS WEB PADA SEKUEN GENOM PARSIAL SAGU (Metroxylon sagu Rottb.) Purwoko, Devit; Cartealy, Imam Civi; Tajuddin, Teuku; Dinarti, Diny; Sudarsono, Sudarsono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1161.678 KB) | DOI: 10.29122/jbbi.v5i1.2878

Abstract

WEB-based bioinformatic analysis on partial genome sequence of Sago (Metroxylon sagu Rottb.)ABSTRACTSago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequences were analyzed using Blast2Go to determine the genetic function annotation, putative gene identification was performed on the Arabidopsis database using the BLASTx program with a 10-3 e-value limit on The Arabidopsis Information Resource (TAIR) (http://www.arabidopsis.org/index.jsp). Gene interactions were analyzed using DAVID and GeneMania programs. Based on sequence analysis with Blast2Go, 33 sequences with Blastx hit consisting of: 29 sequences had a high homology. The sago sequences with a similarity of ≥ 90% are glutamate decarboxylase and HT1-like serine threonine kinase with hit number 10. The distribution of interactions between genes from GeneMania analysis is known to be mostly interconnected in the 65.13% protein domain, predicted 19.83%, genes with 14.47% shared expression and the remaining 0.57% had localization together.Keywords: bioinformatics, gene annotation, gene ontology, genome sequence, Metroxylon sagu ABSTRAKKajian analisis sekuen genom sagu hingga saat ini masih amat terbatas. Penelitian ini merupakan riset pendahuluan analisis sekuen sagu yang diperoleh dari teknologi NGS untuk mengetahui dan mengidentifikasi sekuen gen baru yang memiliki homologi dengan gen pada database NCBI. Sekuen dianalisis menggunakan perangkat Blast2Go untuk mengetahui anotasi fungsional gen, identifikasi gen putatif dilakukan terhadap database Arabidopsis menggunakan program BLASTx dengan batas e-value 10-3 pada The Arabidopsis Information Resource (TAIR). Interaksi gen dianalisis menggunakan program DAVID dan GeneMania. Berdasarkan analisis sekuen dengan Blast2Go, diperoleh 33 sekuen dengan Blastx hit yang terdiri atas: 29 sekuen memiliki homologi yang tinggi. Gen dengan rataan kemiripan ≥ 90% adalah glutamate decarboxylase dan serine threonine-kinase HT1-like dengan jumlah hit 10. Persebaran interaksi antar gen hasil analisis GeneMania diketahui sebagian besar saling terkait pada domain protein 65,13%, koneksi yang berhasil diprediksi 19,83%, gen dengan ekspresi bersama 14,47% dan sisanya 0,57% memiliki peranan bersama. Kata Kunci: anotasi gen, bioinformatika, Metroxylon sagu, ontologi gen, sekuen genome 
PENGARUH PERLAKUAN BENIH DENGAN AGENS HAYATI TERHADAP PERTUMBUHAN, HASIL PADI, DAN PENGENDALIAN PENYAKIT HAWAR DAUN BAKTERI DI RUMAH KACA Agustiansyah, Agustiansyah; Ilyas, Satriyas; Sudarsono, Sudarsono; Machmud, Muhammad
JURNAL AGROTROPIKA Vol 16, No 2 (2011): Agrotropika Vol.16 No.2 2011
Publisher : JURNAL AGROTROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.896 KB)

Abstract

The objective of this research was to study the influence of biological seed treatment on plant growth, yield of rice, and controlling of bacterial leaf blight. The research was conducted at green house of Indonesian Center for Agricultural Biotechnology and Genetic Resources Development, Bogor and Seed Science and Technology Laboratory, Department of Agronomy IPB from August 2009 to Febru-ary 2010. The result showed that biological seed treatment of Pseudomonas spp. and Bacillus spp. (with and without matriconditioning) could increase plant growth of rice in terms of on plant height, number of seedling, root length, root fresh weight, root dry weight, shoot fresh weight, and shoot dry weight. Seed treatments of matriconditioning + P.aeruginosa, matriconditioning + B.subtillis5/B, and Bacillus subtillis 11/C were the best seed treatments in increasing yield of rice as indicated by the highest number of filled grains/panicle, percentage of filled grain/panicle, percentage of filled grain/plant, and the lowest number of unfilled grain/panicle, percentage of unfilled grain/panicle, and percentage unfilled grain/plant. Seed treatments of Pseudomonas diminuta, matriconditioning + Pseudomonas diminuta and matriconditioning + Bacillus subtilis 11/C were significantly resulted in lower percentage of pathogen diseased leaf area (%DLA) than others seed treatment. Percentages of pathogen diseased leaf area were 15.45%, 15.94% and 19.55%. Key words: Bacillus spp Biological seed treatment, matriconditioning, Pseudomonas spp.
Potensi Hasil dan Keragaman Fenotipik Aren (Arenga pinnata (Wurmb) Merr.) Sulawesi Tenggara Fatsan, Ahmad; Sudarsono, Sudarsono; Dinarti, Diny; Maskromo, Ismail
Berkala Penelitian Agronomi Vol 8, No 2 (2020)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/bpa.v8i2.14544

Abstract

Aren adalah salah satu jenis palma yang penyebarannya sangat luas di Indonesia, termasuk di provinsi SulawesiTenggara. Pohon aren memiliki banyak manfaat mulai dari akar sampai daun. Bunga aren berbentuk mayang denganuntaian yang berlapis-lapis. Pengamatan fenotipik dan potensi nira aren dilakukan di tiga Kabupaten yaitu Muna, KolakaTimur dan Bombana.Pengamatan potensi aren Sulawesi Tenggara dilakukan dengan cara mengambil sampel 5 pohonaren di Kabupaten Muna, Kolaka Timur dan Bombana. Parameter karakter fenotipik meliputi lingkar batang dankomponen buah serta potensi poduksi. Hasil uji t-test pengamatan fenotipik aren Sulawesi Tenggara menunjukkanperbedaan yang signifikan pada karakter-karakter fase generatif, terutama aren Muna. Aren Bombana memiliki %brix20.6% lebih baik bila dibandingkan dengan aren Muna dan Kolaka Timur. Produksi rata-rata nira Muna lebih tinggidibandingkan kabupaten lainnya dengan rata-rata produksi23.64 l/mayang/hari sedangkan produksi rata-rata arenSulawesi Tenggara 20.65-23.64 l/mayang/hari.Kata kunci : Aren, Brix, Fenotipik, Generatif,Mayang, Nira
Tolerance of Several Peanut Cultivars against Drought Stress AHMAD RIDUAN; HAJRIAL ASWIDINNOOR; JAJAH KOSWARA; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.019 KB) | DOI: 10.4308/hjb.12.1.28

Abstract

The objectives of this experiments were to determine the effects of drought stress initiated at vegetative growth stage on yield of peanut, to evaluate the tolerance of six peanut cultivars against drought stress, and to determine their leaf prolin and total sugar content. One group of peanut plants were grown in plastic pots and subjected to stress condition during the period of 15-45 days (Experiment I) or 15-80 days (Experiment II) by watering them only in every 4-7 days and were harvested at days 75 (Experiment I) or at maturity (Experiment II). The other group was grown optimally in plastic pot up to harvest period. Leaf prolin content was determined at 12 (before stress), 30 (after three periods of stress), 47 (after six periods of stress), and 64 days in Experiment I and only after six periods of drought stress in Experiment II. Results indicated that drought stress that was initiated at vegetative growth stage reduced plant height, branches number, shoot and root dry weight, and peanut yield. Sensitivity index calculated based on peanut yield showed that peanut cv. Jerapah and Singa were a medium tolerance while Gajah, Kelinci, Macan, and Simpai were sensitive against drought stress. Higher increased in leaf proline content after drought stress was observed in peanut cv. Jerapah and Singa, while less increased was observed in peanut cv. Gajah, Kelinci, Macan and Simpai. Total leaf sugar content of peanut cv. Jerapah, Gajah, Macan and Simpai decreased under drought stress while that of Singa and Kelinci were the same as that under optimal condition. The ability to increase leaf proline content and maintain total leaf sugar content under drought stress could be used as indicators of drought tolerance in peanut cultivars.
In Vitro Selection of Peanut Somatic Embryos on Medium Containing Culture Filtrate of Sclerotium rolfsii and Plantlet Regeneration YUSNITA YUSNITA; WIDODO WIDODO; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.487 KB) | DOI: 10.4308/hjb.12.2.50

Abstract

Attempts to identify somaclonal variants of peanut with resistance to Sclerotium stem rot disease due to infection of S. rolfsii were conducted. The objectives of this study were to develop in vitro selection method using culture filtrates of S. rolfsii, identify culture filtrate-insensitive somatic embryo (SE) of peanut after in vitro selection and regenerate peanut R0 lines originated from culture filtrate-insensitive SE. To achieve these objectives, peanut embryogenic tissues were cultured on selective medium containing various concentrations of S. rolfsii culture filtrates and sublethal concentration of the filtrates. Medium containing sublethal level of S. rolfsii culture filtrates was used to identify culture filtrate-insensitive SE of peanut. Subsequently, the selected SEs were germinated, plantlets were regenerated and preliminary tested against S. rolfsii. Results of the experiments showed that addition of S. rolfsii culture filtrates into medium for inducing peanut somatic embryos drastically reduced their growth and proliferation. S. rolfsii culture filtrates at 10% concentration has significantly reduced the number of proliferated SE per explant. However, sublethal level was achieved at 30% of culture filtrates concentration. Responses of five peanut cultivars against 30% of culture filtrates were similar, indicating they were similar in their susceptibility against S. rolfsii. A number of culture filtrate-insensitive SE were identified after culturing 1500 clumps of embryogenic tissue of peanut cv. Kelinci for three consecutive passages on medium containing 30% of culture filtrates. Germination of selected SE and regeneration of plantlet from culture filtrate-insensitive SE resulted in 50 peanut R0 lines. These lines have been grown in the plastic house and produced normal seeds for further evaluation. Results of S. rolfsii inoculation indicated the existence of chimera for insensitivity against S. rolfsii.
Yield Potential of Ten Peanut Introgression Lines derived from Crosses between Arachis cardenassii and A. hypogaea AHMAD RIDUAN; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 12 No. 3 (2005): September 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (32.526 KB) | DOI: 10.4308/hjb.12.3.116

Abstract

Diploid species of peanut (Arachis cardenasii) showed no symptoms of PStV infection when mechanically inoculated with PStV. Some introgression lines derived from A. cardenasii and A. hypogaea hybridization have been introduced to Indonesia. Evaluation of their adaptability and yield potential were necessary before pursuing further utilization of these introgression lines. The objectives of this research were to determine yield potential of the introgression lines of peanut in green house and field conditions and to evaluate incidence of PStV infection in the field. Peanut plants were grown in the green house and in the field according to standard procedures for raising peanut. Results of the experiments showed that growth and developmental characters of the tested lines were similar between field and green house grown plants. The introgression lines generally exhibited higher secondary branches and longer to flower and harvest as compared to peanut cv. Gajah and Kelinci. The NC-CS30 line was identfied as having higher yield and bigger seed size as compared to standard peanut cultivars (Gajah and Kelinci). Therefore, NC-CS30 germplasm may be further developed as commercial peanut cultivar or be used as donor for peanut breeding in Indonesia.
Regeneration Capacity of Indica Rice cv. Bengawan Solo in Two Types of Regeneration Media through Microprojectile Bombardment TRI JOKO SANTOSO; SUDARSONO SUDARSONO; HAJRIAL ASWIDINNOOR; IDA HANARIDA SOMANTRI
HAYATI Journal of Biosciences Vol. 12 No. 4 (2005): December 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.957 KB) | DOI: 10.4308/hjb.12.4.157

Abstract

Factors affecting the success of gene delivery into cells/tissues of rice mediated by particle bombardment have been investigated. However, their effects on regeneration capacity of rice explants have not been investigated. Objectives of this experiments were to evaluate the effects of particle bombardment on regeneration capacity of indica rice cv. Bengawan Solo calli and the effects of four regeneration media on the development of bombardment calli. Calli of indica rice were initiated from mature seeds on callus-inducing medium. They were transferred on osmoticum containing medium prior to bombardment and maintained on the medium up to two days. Particle bombardment was conducted at 16 cm bombardment distance, 27 cm Hg helium pressure and using standard procedures for preparation of microprojectile particles. The bombarded calli were cultured on RNB or RMS regeneration medium, with or without spermidine addition. Results of the experiment indicated particle bombardment affected the survival of explants of indica rice but did not affect the development of the explants. The development of explants was rather dependent on the regeneration media than on the particle bombardment treatment. RMS medium was better than RNB medium for shoots inducing from bombarded indica rice explants.
Chitinase Activity in Healthy and Sclerotium rolfsii Infected Peanut ENDANG PUDJIHARTATI; SISWANTO SISWANTO; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 2 (2006): June 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.34 KB) | DOI: 10.4308/hjb.13.2.73

Abstract

The objectives of this experiment were to analyze the endo- or exo-chitinase activities of healthy and Sclerotium rolfsii infected peanuts. The experiment analyzed 24 different peanut genotypes. Results of the experiment showed chromogenic dimer was the most suitable substrate for analysing chitinase activities. Both endo- and exo-chitinases activities were detected in leaf, stem, and crown tissues. Increased in chitinase activities were detected in S. rolfsii infected peanut tissues than in healthy plant. Regression analysis showed negative slope between disease intensity and chitinase activity in S. rolfsii infected peanut tissue (R2= 0.45). Key words: Disease response, stem rot disease, endo- and exo-chitinase
Oxidative Burst, Peroxidase Activity, and Lignin Content of Sclerotium rolfsii Infected Peanut Tissue ENDANG PUDJIHARTATI; SATRIYAS ILYAS; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 13 No. 4 (2006): December 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.142 KB) | DOI: 10.4308/hjb.13.4.166

Abstract

The objectives of this experiment were to analyse physiological responses, such as oxidative burst reaction, peroxidase activity, and lignin content of healthy and S. rolfsii-infected peanut tissues. Differences in physiological responses among 24 peanut genotypes were determined, the disease severity was calculated and used to group resistance of tested genotypes. The regressions among observed peroxidase activity, lignin content and disease severity were used to determine the possible mechanisms of S. rolfsii resistance in peanut. Peanut seeds were grown in polybag and the growing plants were inoculated at the crown, stem, and leaf tissues. Results of the experiment indicated that infection of S. rolfsii in peanut did not induce oxidative burst. However, infection of the pathogen resulted in increased peroxidase activity and lignin content in the infected tissues. Regression analysis between peroxidase activity and disease severity showed negative slopes, indicating the more resistance the genotype, the more peroxidase activity in the tissue. Regression analysis between lignin content and disease severity was not significant. Key words: hypersensitive response (HR), resistance mechanisms, Sclerotium stem rot, disease response, Arachis hypogaea
In Vitro Selection of Abaca for Resistance to Fusarium oxysporum f.sp. cubense RULLY DYAH PURWATI; SAID HARRAN; SUDARSONO SUDARSONO
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.252 KB) | DOI: 10.4308/hjb.14.2.65

Abstract

Abaca (Musa textilis Nee) is an important industrial crop. However, the cultivation of this crop in Indonesia is hampered by Fusarium wilt (Panama disease) as a result of Fusarium oxysporum f.sp. cubense (Foc) infection. The objectives of this study were to (i) evaluate inhibitory effects of culture filtrates (CF) of three Foc isolates (Banyuwangi, Malang, and Bojonegoro isolates) on shoot growth of abaca cv. Tangongon and Sangihe-1, (ii) determine sublethal concentration of Foc CF, (iii) isolate variant cells/tissues which are insensitive against Foc CF and regenerate plantlets from the variants, and (iv) evaluate responses of the plantlets against Foc infection. The results of the experiment showed that even though CF of all Foc isolates inhibited abaca’s shoot growth, CF of Foc Banyuwangi isolate showed the most inhibitory effect. Sublethal concentration of CF of Foc Banyuwangi isolate was 40%. From abaca cv. Tangongon, 326 shoots were regenerated from CF insensitive embryogenic calli while from Sangihe-1 - 176 shoots were regenerated. Following acclimatization and Foc inoculation using detached-leaf dual culture test, a total of four immune, two resistant, and two moderately resistant plantlets were identified out of 45 tested variants of Tangongon. On the other hand, only two resistant and one moderately resistant plantlets were identified out of 10 tested variants of Sangihe-1. Key words: Fusarium wilt, in vitro selection, culture filtrate, ethylmethanesulphonate (EMS), Musa textilis Nee
Co-Authors , Agustiansyah , Rustikawati , Sukendah , Triadiati , Witjaksono . DARMAWAN . Giyanto . Iskandar . Kurniati . Suwardi A. Farid .Hemon Abad, Hulud Abd. Kadir Abdul Madjid ABDUL RACHMAD BUDIONO Achwan, Muhammad Ade Mariyam Oklima Ade Mariyam Oklima Ade Wachjar Ade Wachjar Adisyahputra Adisyahputra, Adisyahputra Afandi, Muhammad Yusron Afifulloh, Afifulloh Agung Endro Nugroho Agung Wahyu Susilo Agus Purwito Agus Rachmat Agustini, Rika Yayu Ahmad Fathoni Ahmad Musaddad, Ahmad AHMAD RIDUAN Ahmad Surkati Ahmad, Evi Aksar, Prinob Alfian, Hamzah Ali Husni Amalia, Fadila Amarullah, Muhamad Roffi Ambarwati, Sinta Devi Amelia, Septiyan Nur Aminur Aminur Aminuyati Ananta, Puja Asti Andeas, Andeas Andrie Al Kausar Anita Yuliyanti Aniversari Apriana Anjarsari, Nurul Aeni Anneke Pesik Ansyori, Ansyori Anugrah, Ridho Anwar, Aswaldi Apriliani, Elisa Aprizal Zainal Arfan Nazhri Simamora Arief Hidayat Arif, Mochamad Syaiful arifin tahir Aris Munandar Ariswan, Ariswam Arrosyadi, Muhammad Ilham ASMONO, DWI Aspiana, Endah Asri, Hendri Rahmayani Atang Sutandi Atmitri Sisharmini Ayu Amelia Azhar Nuh, Fuad Bachtera Indarto, Bachtera Bachtiar, Iyas Muzalif Badrut Tamam Bahar Bahar, Bahar Bambang Heliyanto Bambang S. Purwoko BAMBANG SUGIRI Barahima Abbas Bernadetta Octavia Bonadi, Ahmad Budi Santoso, Slamet Budiman Sudia, Budiman Budiwati, Budiwati - C J Soegihardjo Cahyaningrum, Lulik Tri Cahyono, Andy Agus Cartealy, Imam Civi Cartealy, Imam Civi Cecep Kusmana Chairul, Muhammad Christina, Yani Christoph Leuschner Chusni Ansori Clarry Sada Colleen M. Higgins, Colleen M. Darmawan Saptadi Darmawan, Rizqi Wahyu Deka Rotama Desi Pujiati, Desi Desta Wirnas DEWI SARTIKA Dewi Sukma Dhanu, Rusli Didiek Hadjar Goenadi Diky Anggoro Dina Lusianti Dinarti, D DINARTY, DINY Dini Dinarty, Dini DINI NURDIANI Diny Dinarti Djoko Santoso DWI ANDREAS SANTOSA Dwi Asmono DWI HAPSORO Dwi Hapsoro Dwi Riyanti Dwiputra, Adnan Wahyu Dyah Ochtorina Susanti Dyah Tjahyandari Suryaningtyas Edi Guhardja Edwinarta, Caesar Dimas Efendi, A'an Efendi, Aan Efendi, Darda Eka Fajar Rahmani Ekastuti, Zaidatun Elina, Juanita ELSJE T. TENDA, ELSJE T. Endang Pudjihartati Endang Susilawati Endrizal Eneng Fakhrunnisa Enni Suwarsi Rahayu Enung Sri Mulyaningsih Erick Raynalta, Erick Ernata, Yusvidha Eusabinus Bunau Fadli, La Ode Muammar fadoli, hendi imam Fahlefi, Reza Fahmi Astuti, Fahmi Fajar Laksono Suroso Fajri, Adz Dzaa Riaat Fakhrunnisa, Eneng Fatimah, Iim Fatmawati, Nurul Fatsan, Ahmad FETRINA OKTAVIA Fitri Yanti Fredyarini Soedjiwo, Novena Ade Gatot Sutapa Yuliana, Yohanes Gatut Yudoyono Germini Alam Ginting, Tarianna Giyanto . Gontjang Prajitno Grantaka, Akbar Maulana Gusti Ayu Kade Sutariati H Yulipriyanto, H H. M.H. Bintoro Djoefrie HAJRIAL ASWIDINNOOR Hari Pamungkas Hari Sutrisno Harsini ,, Harsini Hartanti, Nenik Diah Hartati, Raden Roro Sri Haryadi Permana Hasanat Uzzahroh, Umi HASIBUAN, HOIRUDDIN Hasriadi Mat Akin Heltty Heltty Heltty, Heltty Hendriyanto, Dennyca Hengky Novarianto Hengky Novarianto Herdhata Agusta Herison, Catur Hernandi, Iman Budi Herwantono, Herwantono Hidayat Pawitan Hiroshi Ehara Hugo Volkaert, Hugo I MADE ARTIKA I Nyoman Nurjaya IBNUL QAYIM IDA HANARIDA SOMANTRI Iflaha, Nurul Iim Fatimah Ika Mariska Ikhsanudin Ikhsanudin Ikhsanudin Ikhsanudin Ikhsanudin Imam Mashudi, Imam Imran, Al Ichlas Indriyati, Lilik Tri Insani, Andi Nur Ipaljri Saputra, Andi Ipaljri, Andi Irawan, Dedy Irfan, Aprian Irman Firmansyah Iskandar Iskandar Ismail Maskromo Ismail Maskromo Ita Nuryana Iwa Sutardjo, Iwa Iwan Permadi Iwan Sasli Iwan Setiawan Izroiel, Rabbenstain JAJAH KOSWARA Jaka Seru Dwi Saputra Jaswadi JASWADI, Jaswadi Juang Gema Kartika Juita, Nirmala Jumanto Jumanto Jumanto Jumanto, Jumanto Jumari Jumari, Jumari Jusman Jusman Kadir, Abd . Khaerunnisa, Aisyah Khoerul Anwar Khursatul Munibah Kukuh Murtilaksono Kukuh Setiawan Kurniasari, Praharisti Kurniawan R. Trijatmiko Kurniawan, Choirul Kusumo Nugroho Kuswanhadi Kuswanhadi, Kuswanhadi La Ode Ahmad Barata Laksmita Prima Santi Latifa, Annisa LATIFAH KOSIM DARUSMAN Lila Yuwana Lila Yuwana Lisa Virdinarti Putra Lollie Agustina P. Putri Lubis, Fatma Hani Luwandi Suhartono M. Herman Maera Zasari Mahrusun, Mahrusun Mamik Indaryani Manalu, Victor Manotar Pademan Marbelisa Briliani Mardikaningsih, Agusti Marenda Ishak S Maria Yosepin Endah Listyowati Mariam Mariam MASKROMO, ISMAIL Masluki, Masluki Mawaddah, Ida Maya Megayani Sri Rahayu MEITY A. TULALO, MEITY A. Memen Surahman Merry Sintia Dewi Meynarti Sari Dewi Ibrahim Miguna, Sarita Misar, Misar Moh. Zaini Muadin, Akhmad Muchammad Ali Safaat Muchlisa M, Dewi Ratna Muchtar, Asmaji Muhammad Arifin MUHAMMAD HASBI Muhammad Hasjim Bintoro Djoefrie Hasjim Bintoro Djoefrie Muhammad Hasyim Bintoro Muhammad Herman MUHAMMAD HERMAN Muhammad Ikhsan Muhammad Kamal Muhammad Machmud Muhammad Syukur Muhammad, Ikram Muhson, Akhmad Mulat, Astuti Musfira, Musfira Musthafa, Annas Rasid Mutia Dewi Yuniati N. Sri Hartati, N. Sri Nanang Endriatno Napitupulu, Tosanov T.P. Narindra, Hawin Neviana Wahyuni Nihayati, Hanik Endang Niniet Indah Arvitrida Nooryanto, Fista Herry NOVARIANTO, HENGKY Nugraheni, Titi Nuh, Fuad Azhar Nur Khayati Nur Wahyudi Nur, Rahmat Nurhajati A. Mattjik Nurmalitasari, Melinda Nurmaya, Alya Nursalam, Nursalam Nurul Khumaida Oktafia, Yeni Omaidi, Omaidi Paat, Johny Christofel Pakpahan, Benhur Pane, Putri Yunita Paulus B.K. Santoso Prihatin, Lilik Prija Djatmika, Prija Prita Sari Dewi Purwaningsih - Purwoko, Agus Purwoko, Devit purwoko, devit Puspitasari, Nurrisma Putra, Adhitya Pratama Putri, Halida Adistya Qodarsih, Inna Anzalina Qosim, Achmad R B Gobel Rachman, Azis Rahmadhani, Tasya Rahman, Wahbi Rahmawati, Rika Sri Rajamanickam, Ramalinggam Ralf G. Dietzgen, Ralf G. Ratnawati Ratnawati Razak, Askari Regina Regina Rehulina Rehulina, Rehulina Renaldi, Muhammad Rizal Reni Indrayanti Rina Wati Rita Lefrida Roedhy Poerwanto Rr Sri Hartati Rr Sri Hartati, Rr Sri Rr. Sri Hartati Rubbi Kurniawan Rubiyo Rubiyo RULLY DYAH PURWATI Rusmayani, Rusmayani RUSMILAH SUSENO Rustan Rykson Situmorang S. Arsyad S. Ilyas Saefudin Saefudin Sahrul, Sahrul Said Harran Saiful Akhyar Lubis Samhuddin Samhuddin, Samhuddin Samidun, Samidun Sandra, Yulia Sari, Nunuk Tujung Sarif, La Sarudin, Sarudin Satriyas Ilyas SATRIYAS ILYAS Satya Nugroho Septanti, Annisya Setiawan, Asep S Setiawan, Rizki Nur Setiowati, Retno Diah Setyaningrum, Diyah Setyarini, Ekaning Setyawan, Anton Afries Setyawan, Fadjar Ramdhani Setyawan, Trinovandhi Shaleha, Niswatun Shandra Amarillis Shinta Hadiyantina Sholeh Avivi Sidiki, Mey Wulansari Silaen, Jonni Haonangan Sintho Wahyuning Ardie Siregar, Vicentius P. Sisworo, Widjang Herry Siti Mufidah siti nurlela Siti Sunarintyas SOEKISMAN TJITROSEMITO Sri H. Hidayat Sri H. Hidayat Sri H. Hidayat Sri Hastutiningrum Sri Indarto Sri S. Harjadi Sri Soenarsih DAS Sri Wening Stephanie Wilamarta Sudari, Sudari Sudibyo Martono Sudirman Yahya Sudirman Yahya SUDJINDRO SUDJINDRO Sudradjat , Sughiandi, Rachmad Sugianto, Pingkan Sugiri, Bambang Sugiyanta Suhandoyo Suhartini Suhartini Suhermanto, Totok Suhirman, Suhirman Sukendah S, Sukendah SUKENDAH SUKENDAH, SUKENDAH Sumali Wiryowidagdo Sumarni Sumarni Surmiyati Surmiyati Suryo Wiyono Susilo Indrawati Susilo Indrawati Sutji Rochaminah Suwargana , Nana Suwarto, Visia Assyafira Suweni Muntini, Melania Suyatno Suyatno Swastika, Maria Swastika, Maria Syafa'at, Rachmad Syafaruddin Syafaruddin Syafi`ullah, Alfian Syarif Husin Tamaulina Br Sembiring Tambunan, Brio Gideon Tarigan, Sabarita Taufik, Beatrice Ellen TENDA, ELSJE T. Tengku Riza Zarzani N Teuku Tajuddin TINCHE, nFn Tri J. Santoso Tri J. Santoso Tri Joko Santoso Tri Joko Santoso Tri Rini Nuringtyas Tyaningrum, Annisa Mayang U.S WIRADISASTRA Ucu Sumirat UJANG KHAIRUL Ulfa, Umi Tahniah urai salam Utomo, Abet Yudho Uzzahroh, Hasanat vyta andri setyo utami Wahdaniyah, Gusti Wahyu Wahyu Wajdi, Muhammad Warsiyah Warsiyah, Warsiyah Wening Enggarini Widiatmaka Widodo Widodo Wipsar Sunu Brams Dwandaru Wisilayasa, I Gede Darma Wiwik Kusmawati, Wiwik Wiwin Ambarwulan Yadi Setiadi Yanti Sri Rejeki Yobel, Christian Yudiwanti Wahyu E. Kusumo Yuliati - Yulis Setiya Dewi Yundari, Yundari Yuni Wibowo Yusnita Yusnita Yustinus Yustinus, Yustinus Yusuf, Rinto Zahalim Zahalim Zahalim, Zahalim