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Journal : Indonesian Journal of Pharmacology and Therapy

Antiplasmodial activity and cytotoxicity of kapur (Harmsiopanax aculeatus) leaf extracts traditionally used for the treatment of malaria in Maluku Turalely, Rachel; Mustofa; Mahardika Agus Wijayanti; Triana Hertiani
Indonesian Journal of Pharmacology and Therapy Vol 1 No 1 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.512

Abstract

Harmsiopanax aculeatus leaf, locally name kapur, has been use traditionally to treat malaria in Maluku, Indonesia. However, the scientific evidences that support its use are still limited. This study aimed to investigate antiplasmodial activity of H. aculeatus leaf extract and its cytotoxicity on cancer cells line. Three extracts i.e. methanolic, n-hexanic and ethyl acetate extracts were evaluated for their in vitro activity against Plasmodium falciparum FCR3 strain using microscopic method. Cytotoxicity of the extracts on T47D, HeLa and Vero cells lines were determined using MTT assay method. The inhibitory concentration 50% (IC50) against P. falciparum or the cells lines growth was determined using probit analysis. Furthermore, their selectivity index (SI) were determined. The results showed that the methanolic extract was the most active extract with an IC50value of 13.82 μg/mL and the most selective with a SI value of 172.84. The three extracts tested exhibited weak or no cytotoxicity against the cells lines used with IC50 values ranged 101-2388.69 μg/mL. Further study will be conducted to isolate active antiplasmodial compounds from the methanol extract.
Cytotoxicity of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) on HeLa cell line Adisty Ridha Damasuri; Eti Nurwening Sholikhah; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 1 No 2 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.606

Abstract

In our previous study, some amino chalcone derivatives have been synthesized and evaluated their cytotoxicity against breast cancer cell line T47D. Among 11 amino chalcone derivatives, ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) exhibited the most active compound.This study aimed to investigate cytotoxic activity of the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) against cervical cell line (HeLa). The cytotoxic activitywas determined using the MTT colorimetric assay. Cisplatin was used as positive control. From this MTT method, inhibitory concentration 50% (IC50) values were determined by probit analysis based on the relationship between log concentrations versus the percentage of cells growth inhibition.The results showed that the IC50 of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) and cisplatinwere 22.75 ± 19.13 μg/mL and 14.96±1.08 μg/mL, respectively. In conclusion, the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) has moderate cytotoxic activity against HeLa cell line based on National Cancer Institute (NCI) criteria.
Synthesis of 3-carbethoxy-4(3’-chloro-4’-hydroxy) phenyl-but-3-en-2-one and its cytotoxicity evaluation against cancer cell carrying mutant p53 Julianus, Jeffry; Mustofa; Jumina
Indonesian Journal of Pharmacology and Therapy Vol 2 No 1 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.1211

Abstract

Overexpression of mutant p53 in cancer cell inactivates the p53 pathways to execute apoptosis and cell cycle arrest. This study aimed to synthesize new kardiena derivative compound, 3-carbethoxy-4(3’-chloro-4’-hydroxy)phenyl-but-3-en-2-one, to reactivate the p53 pathways to execute apoptosis and cell cycle arrest. Characterization of the synthetic compound employing melting point, IR, EI-MS, 1H-NMR, and 13C-NMR spectra revealed 3-carbethoxy-4(3’-chloro-4’-hydroxy)phenyl-but-3-en-2-one was successfully synthesized from 3-chloro-4-hydroxybenzaldehyde and ethyl acetoacetate using dimethylamine as a catalyst. This compound had antiproliferative activity against the WiDr cells which carried mutant p53. Its antiproliferative activity was better than 5’-FU as a reference standard to treat colon cancer. Increasing WiDr cell accumulation in the G2-M phase, the active form of caspase-3, and inducing apoptosis demonstrated the ability of 3-carbethoxy-4(3’-chloro-4’-hydroxy)phenyl-but-3-en-2-one to reactivate p53 pathways to execute apoptosis and cell cycle arrest in cancer cells carrying mutant p53.
Antiplasmodial activity and genome mining study of marine-derived Streptomyces sp. GMY01 Damayanti, Ema; Widada, Jaka; Dinoto, Achmad; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 2 No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.1335

Abstract

High resistance to chloroquine in most malaria-endemic area in the world leads to the need for new antimalaria drugs. Marine bacterium Streptomyces is the source for potential new antimalarial molecules. This research aimed to investigate antiplasmodial activity of marine-derived of Streptomyces sp. GMY01 and to identify potential active compounds using genome mining study. In vitro antiplasmodial activity assays using flow cytometry method showed that the ethyl acetate extract of this bacterium had high antiplasmodial activity (IC50 value of 1.183 µg/mL) on Plasmodium falciparum FCR3. Genome mining analysis of whole-genome sequences using antiSMASH 6.0 beta version revealed that Streptomyces sp. GMY01 had 28 biosynthetic gene clusters (BGCs), including the genes encoding polyketide synthase, non-ribosomal peptide synthetase, terpene, lanthipeptide, bacteriocin, butyrolactone, ectoin, siderophore, and others. The known BGCs were predicted to be involved in the production of known compounds from gene clusters ranged from 5 to 100% similarity. Ongoing purification and elucidation of the structures will allow identification of the active compounds produced by marine-derived Streptomyces sp. GMY01.
Antimicrobial activity of Pycnarrhena cauliflora (Miers.) Diels. methanol extract Eti Nurwening Sholikhah; Maulina Diah; Mustofa; Masriani; Susi Iravati; Samekto Wibowo
Indonesian Journal of Pharmacology and Therapy Vol 2 No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.1656

Abstract

Pycnarrhena cauliflora (Miers.) Diels., local name sengkubak, is one of indigenous plants from West Kalimantan that has been used as natural flavor. Pycnorrhena cauliflora is one of species of Menispermaceae family which is rich in bisbenzylisoquinoline alkaloids. This alkaloids are known to have various biological activities including antiprotozoal, antiplasmodial, antifungal and antibacterial activities. This study aimed to investigate antimicrobial activity of the P. cauliflora (Miers.) Diels. methanolic extracts against gram-positive and gram-negative bacteria. The methanolic extract of P. cauliflora (Miers.) Diels., root, leaf and stem were prepared by maceration. The disk-diffusion method was then used to determine the antimicrobial activity of the extracts against Streptococcus pyogenes, S. mutants, Staphylococcus aureus, S. epidermidis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Escherichia coli after 18-24 h incubation at 37 oC. Amoxicillin was used as positive control for gram-positive bacteria and ciprofloxacin was used as gram-negative bacteria. The inhibition zones were then measured in mm. Analysis were conducted in duplicates. The results showed in general the methanolic extracts of P. cauliflora (Miers.) Diels. root (inhibition zone diameter= 10-23 mm) were more active than that leaf (0-15 mm) and stem (0-17 mm) extracts against gram-positive bacteria. The zone inhibition diameter of amoxicillin as positive control was 8-42 mm. In addition, the methanolic extracts of P. cauliflora (Miers.) Diels. root (12-17 mm) were also more active than that leaf (0-12 mm) and stem (0-12 mm) extracts against gram-negative bacteria. The zone inhibition diameter of ciprofloxacin as positive control was 33-36 mm. In conclusion, the methanolic extract of P. caulifloria (Miers.) Diels. root is the most extract active against both gram-positive and gram-negative bacteria. Further study will be focused to isolate active compounds in the methanolic extract of the root.
The effect of Centella asiatica L. Urban. and Curcuma longa L. extracts combination in improving memory performance in stroke model rats and its acute toxicity Gofir, Abdul; Ar Rochmah, Mawaddah; Wibowo, Samekto; Hakimi, Mohammad; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 2 No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.1765

Abstract

Post-stroke cognitive impairment involves memory, visuoconstructional, and spatial dysfunctions. Centella asiatica L. Urban. and Curcuma longa L. are both well-known herbs in South and South-East Asia countries that are believed to possess neuroprotective properties as memory enhancers. This study aimed to investigate the effects of C. asiatica L. Urban. and C. longa L. extracts combination in improving memory performance in stroke model rats and its acute toxicity. Twenty-five Wistar rats underwent transient bilateral common carotid artery occlusion. Y-maze pre-test was performed 24 h after the occlusion. The rats were then divided into five groups i.e. Group 1 received NaCMC dilution, Group 2 received donepezil 0. 7 mg/kg BW/day, and Groups 3, 4, and 5 received the extracts combination with dose of 59; 118; and 236 mg/kg BW/day, respectively. Y-maze post-test was performed 24 h after the last dose had been given. Spontaneous alternation behavior was used as the indicator of working memory measurement. The fixed-dose method according to OECD Guideline was used to estimate the LD50 in acute toxicity testing on Wistar rats. There was a significant difference in the delta spontaneous alternation percentage among groups tested (p<0. 05). Group 1 had significant difference with any other group (compared to Groups 2 to 5; p<0.05). No significant difference could be found between groups of the extracts combination and donepezil group suggesting that the effect is not more inferior nor dose-dependent in improving memory performance. The extracts combination at a single dose of 2000 mg/kg BW did not show related signs of toxicity or mortality in any of the rats during the 14-day observation period. In conclusion, the C. asiatica L. Urban. and C. longa L. extracts combination can improve performance of memory on stroke model animal. According to Globally Harmonized Classification System, the extracts combination can be classified as Category 5/unclassified.
Liposome as carrier therapy for Alzheimer’s disease Anggraeni, Andi Sri Dewi; Arsita, Emilia Vivi; Suwarman, Adika; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 3 No 2 (2022)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.2315

Abstract

Alzheimer's disease (AD) is the most common type of dementia. Approximately 50 million people suffer from Alzheimer's and it is expected to increase more than 100 million by 2050. According to the Diagnostic and Statistical Manual of Mental Disorders V (DSM-V), the diagnosis of AD has an onset of memory impairment and cognitive decline. Effective treatment of AD is a major challenge in the field of pharmacy and science due to the restriction from blood brain barrier (BBB). This causes poor access to pills or oral administration. Liposome as a part of nanoparticle showed to be a potential AD treatment because of its biocompatibility, flexibility, and capability to carry various therapeutic molecules across the BBB and into brain cells. Several types of liposomes provide varied therapy effect. Some factors which determine liposomes effectiveness are its structure, mechanism of action; formed; and delivery, and its preparation method. This article reviewed liposomes characteristics which has potency as carrier therapy for AD.
Molecular docking study of Indonesian phytochemicals as inhibitor of 6-HB formation by binding to HR1 subunit of SARSCOV-2 S2 protein Julianus, Jeffry; Phebe Hendra; Maywan Hariono; Nico Frederick; Satrio Ardyantoro; Mustofa; Jumina
Indonesian Journal of Pharmacology and Therapy Vol 2 No 3 (2021): Special Issue: COVID-19
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.2392

Abstract

Coronavirus disease 2019 (COVID-19) is still as global pandemic. No officially drugs to treat COVID-19 are available until now. Increasing number of patients and deaths trigger new antiCOVID-19 discovery efforts. In this study, we have conducted in silico screening employing molecular docking for Indonesian phytochemicals. Docking process was performed by employing AutoDock4 software on crystal structure 6VSB. Based on molecular docking results, several compounds had potential as antiCOVID-19 drugs, such as β-carotene, veramiline, ecliptalbine, betulinic acid, and lupeol. β-carotene was the most potential compound to treat COVID-19.
Cytotoxic activity of hantap (Sterculia oblongata Mast) leaves extract against breast cancer cells line (MCF7/HER2): the effect on the expression of HER2 mRNA and the apoptosis Sitti Ayu Suhartina Yahya; Mustofa; Woro Rukmi Pratiwi; Adika Suwarman
Indonesian Journal of Pharmacology and Therapy Vol 3 No 1 (2022)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.3204

Abstract

Hantap leaves (Sterculia oblongata Mast) has been used traditionally to treat breast cancer in Palu, Central Sulawesi. However, its use is just based on empirical evidences rather than scientific evidences. The study aimed to investigate the cytotoxic activity of hantap leaves extracts against breast cancer cells line. The effect of this extract on the HER2 expression and the apoptosis was also evaluated. The hantap (S. oblongata Mast) leaves extracts were prepared by consecutive maceration method using n-hexane, methanol and water, respectively. The cytotoxic activity against MCF7/HER2 breast cancer cells line was evaluated using the MTT assay with doxorubicin as a positive control. The HER2 mRNA expression was examined using RT-PCR and the apoptosis after 24 h incubation was examined using a fluorescence microscope after AO-PI (acridine orange-propidium iodide) staining. Among three extracts tested, the methanolic extract exhibited the most cytotoxic against MCF7/HER2 cells with an IC50 of 91.25 μg/mL. Therefore, the methanolic extract was subjected to further study. The methanolic extract at concentration of 1/2IC50; 1IC50 and 2IC50 μM induced 6.8; 26.3 and 25.3% apoptosis of the MFC7/HER2 cell lines, respectively. The methanolic extract at concentration of 1/2IC50; 1IC50 and 2IC50 μM inhibited HER2 mRNA expression to be 0.6; 0.25 and 0.33 compared to control cells. In conclusion, the methanolic extract of hantap leaves (S. oblongata Mast) has cytotoxic activity against MCF7/HER2 breast cancer cell lines by induce cells apoptosis and inhibit HER2 mRNA expression. Further study, will be conducted to isolate active constituents as anticancer.
Potential active compounds of Streptomyces sennicomposti GMY01 for antiplasmodial and antiSARS-CoV-2 revealed by targeted metabolomic and molecular docking Damayanti, Ema; Nisa, Khoirun; Febriansah , Rifki; Hadi, Ismanurrahman; Dinoto, Achmad; Widada, Jaka; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 5 No 3 (2024)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.9825

Abstract

Streptomyces sennicomposti GMY01 is a bacterium with huge biotechnological potential that revealed by genome mining analysis. This research aimed to investigate the potential compounds as antiplasmodial and the antiSARS-CoV-2 from the S. sennicomposti GMY01 using targeted metabolomic and in silico molecular docking. The crude extract was obtained by extraction of supernatant from fermentation product of the S. sennicomposti GMY01. The secondary metabolite profiling was obtained by using ultra-high-performance liquid chromatography (UHPLC) coupled to targeted high-performance mass spectrometry (HRMS) based on genome mining data of whole genome sequence (WGS). In silico molecular docking was performed on important target protein of P. falciparum i.e. glutathione reductase (PfGR), lactate dehydrogenase (PfLDH), phosphoethanolamine methyltransferase (Pfpmt), erythrocyte membrane protein 1 (PfEMP1) and glutathione-S-transferase (PfGST); and of SARS-CoV-2 proteins i.e. protease domain, spike glycoprotein, receptor-binding domain angiotensin-converting enzyme 2 (RBD-ACE2), 3-chymotrypsin-like protease (3CLpro), and RNA-dependent RNA polymerase (RdRp). One compound from S. sennicomposti GMY01 extract, albaflavenone was confirmed by targeted LC-HRMS. On molecular docking analysis, albaflavenone showed higher affinity than chloroquine as antiplasmodial drug and exhibited same affinity to remdesivir as antiSARS-CoV-2. Stertomyces sennicomposti GMY01 has promising biotechnological potential for drug development as antiplasmodial and anti-SARS-CoV-2 agent. Further study is needed, especially regarding in vitro testing of albaflavenone as antiplasmodial and antiSARS-CoV2.
Co-Authors -, Nasiri A Halim A. Fitriani Abdilah, Ulil Absor Faiq Abdul Gofir Abdul Hakim, Al Hasan Abdul Kholik Abdul Manaf Ali, Abdul Manaf Abdul Rokhim Abdul Wahid Arohman Abdullah, Rizky Achmad Dinoto Achmad Sehan Ade Nur Rohim Ade Setiawan Adi Putranto, Hanafi Adika Suwarman Adisty Ridha Damasuri Afib Rulyansah Agus Faisal Asyha Agus Pahrudin agus prasetyo aji Agus Sugiarto Agussalim Agussalim, Agussalim Agustin HP, Agustin Agustina, Feny Rizki Eka Ahmad Asrof Fitri Ahmad Hamim Sadewa Ahmad Khoirul Fata Ahmad Nouvel - AMIK BSI Purwokerto Ahmad Nouvel - AMIK BSI Purwokerto Ahmad Syaifuddin Romli Akhmad Akbar Susamto Akhwani Akhwani Akhwani, Akhwani Akrom, Akrom Akuba, Syamsu Alatas, Haidar Alexis Valentin, Alexis Alfi Yasmina Alfinatus Zayyinah Alfiyah, Viny Ali Djamhuri Ali Ghufron Mukti Ali Muhson alimin alimin Almubarokah, Nurul Hidayah Almurni, Muhammad Furqon Amri, Aini Nurul Andayani, Tri M. Andi Hairil Alimuddin Andi Namirah Muh. Syuaib AS, Andi Andini Hardiningrum Andrian Saputra Ange Desire Yapi, Ange Desire Anggraeni, Andi Sri Dewi Ani Widayati Ansori, Mukhamad Yusuf Apriani, Hikma ARDIANSYAH, JEFRI Arfiani Nur Arief Agoestanto Arief Budiyanto Arif Budi Setianto Arif Budiman Arina Selawati Arini Arini Aristia, Bella Fevi ariyanti, firda aulia Arsita, Emilia Vivi Arum Darmawati Arviyah, Vira Asep Kurniawan Asri Widowati Astuti, Febriana Astuti, Rachma Darma Astuti, Rachmawati Darma Atikah, Ika Audita Nuvriasari Aufa, Mohammad Zidan Aula Ahmad Hafidh Saiful Fikri Awal Syahrani Sirajuddin AYU WULANDARI Azizah, Nuryana Elia Badrudin Baijuri Baso Intang Sappaile, Baso Intang Basri Basri Bawazier, Lucky Aziza Bayu Prio Septiantoro Bayu Supriyadi Brilian, Dion Nicky Budi Sasomo, Budi Budi Tresnayadi Caesar Ananta Putra, Ihrom Catur Sugiyanto Chairil Anwar Chintyasari, Shaula Curatman, Aang Dadi Mahardi Dafitri, Rizkika Daniel Parenden, Daniel Danny Syamsu W Danto Danto, Danto Darmawan, Rio Surya Daru Wahyuni Dede Nurwahidah Dedy Wijaya Kusuma Desi Suci Handayani Dessy Susanti, Inggit Destita Shari Desy Agustin Dewi Sadiah, Dewi Dewi Sulistiyarini Dewi, Wulan Rahma Dhina Fitriastuti Diana Diana Diana Dwi Astuti Djoko Wahyono Donant Alananto Iskandar, Donant Alananto Dwi Puryanti Dwi Siswanta Dwi Widiastuti, Dwi Dyah Aryani Perwitasari Edy Turjono Eka Ary Wibawa Eko Budiono Ema Damayanti Emmy Hamidah Encep Taufik Rahman Endah Puji Septisetyani, Endah Puji Endang Darmawan Ending Solehudin Erna Kristin Eti Nurwening Sholikhah Eva Rahmawati Evi Mulyani Evi Sunarti Antu Fadhilah, Putry Fahrezi, Fajar Fahrur Razi Faisal, Agus Faiz, Afiyatul Mahfudloh Fandi Dwi Imaniawan, Fabriyan Faqih, Muhammad Izzatul Fathur Rozi Fatmawati, Risdiana Andika Febriansah , Rifki Fernanda Al Welery Fitranto Arjadi Fredie Irijanto Gigih Hilmiawan Gilang Pratama Gunawan Gunawan Gunawan H. Soro , Suharyanto Hadi, Aminulloh Hadi, Ismanurrahman Hafizah, Noor Haifah, Haifah Haitham Hanifah Yusuf Haqiqi, Adif Farhian Harini, Ika Murti Hariyadi, Ari HARIYANTO HARIYANTO Haryadi Hasan, Zainol Hayatul Maspufah Heny Ekawati Haryono Heriyanto Heriyanto Heru Agus Cahyanto, Heru Agus Heru Purwanto Hidetaka Kawakita Hisam Ahyani Holil Houtman I Dewa Putu Pramantara Setiabudi I Nurol Aen Idris, Irma Surya Kumala Idris, M. Manshur IGP Suryadarma Iis Nur Asyiah Ika Puspita Sari Ilmiyah, Izzatul Imam Haryono Indah Purwantini Indah Rahmawati Indwiani Astuti Iqmal Tahir Irawan Irawan Irfansyah, Putra Isa, Abdullah Iskandar Iskandar Iskandar Ritonga Isnaini Maulidatu Nisa izza, Aqidatul JAKA WIDADA Jamaludin, Jujun Jastria Pusmarani Jauharotur Rihlah Jayawarsa, A.A. Ketut Jeffry Julianus Jefry Tarantang Jenny Hidayat Joko Waluyo Jordy Lasmana Putra Jubaedah, Dedah Jumina Jumina Kamid Kamid Kasduni Keisuke Ohto Kennedy, Kennedy Khasanah, Uswatul Khoirul Fata, Khoirul Khoirun Nisa Khotib Khotib Khotib, Khotib Khusniyah, Siti Alfitrotul Kistiananingsih, Isti Kristian Selleng Kuncoro, Prasetyo Tri Kurani Mega Asteroid Kurnaesih, Uun Kurnia, Heri Kushariyadi Kushariyadi Kusuma, Nurul Rahmah Kusumasari, Dyah Kusumawati, Nyimas Inda Lai, Kenny Latif, Lita Asyriati Leo Soemardji Lilia Pasca Riani Listini Listini, Listini Lorettha Wijaya Losina Purnastuti Luluk Faridah Lutfi Fahrul Rizal, Lutfi Fahrul M Rusdi M, Nurdiana M. Satrio Setia Budi Madani, Arya Indra Mafrudlo, Ahmad Mahfudzi Maftuh, Abdullah Maghfiroh, Annisatul Mahardika Agus Wijayanti Mahardika Agus Wijayanti Maimun Sholeh Makki, Hali Malasari, Putri Nur Mansur, Zuhratul Aini Marhadi Saputro Mariani, Scholastika Marlinda, Dwi Marstyawan Marstyawan Masriani . Masrid Pikoli Maulina Diah Mawaddah Ar-Rochmah Maywan Hariono Medi, Gery Meirina Suci Ridha Melia, Anissa Dwi Melia, Annisa Dwi Meti Fatimah Mintarsih, Betty Mintarsih, Betty Misba Huddin Mislan Moh. As’ad Mohamad, Roni Mohammad Hakimi Mubarika Mubarika Muchsin Muchsin Muflikhah, Khusnul Mufrida, Farah Fergi Muhammad Idham Darussalam Mardjan Muhammad Nur, Hidayat Muharir, Muharir Muharromah, Gabriele Lailatul Mujahidawati, Mujahidawati Mukammilah, Mukammilah Mukhlas, Oyo Sunaryo Mulyadi Mulyadi Mulyani Surendra Mulyono Mulyono Mumtaza, Alif Nafiatul Munawar, Agus Muqoddaroh, Fadillatul mursid, fadillah Musaiyadi Musaiyadi Musawir, Musawir Musriatun Napiah Musri’ah, Musri’ah Mustofa, Ismail Muthi Ikawati Muvita Rina Wati Nafi'ah, Nafi'ah Nahadia, Risna Nasiruddin, Atsir Faris Nasrudin Nazudin, Nazudin Nenden Susilowati NETTI KARIANI MENDOFA Nico Frederick Nisa, Shinta Khoirun Nisak, Sayida Khoiratun Nita Kusumawardani Nita Suleman Nofia Sari, Okta Noval Cahyo Triyanto Noventi, Iis Novita Carolia Nugraha, Raffy Ave Nur Rochmat Nur Signa Aini Gumilas Nuraini El Fajri Nurbaiti Nurbaiti Nurhadi, Hikari Qurrata'ain Nurhayati Nurhayati Nurhayati, Is Nuriman Nuriman, Agus Nurkolis Nurkolis, Nurkolis Nurmaulida, Regita Okti Nurrohman Nurrohman Nursari, Nina Nursyahira, Kasandra NURUL FAIZAH Nuryani Nuryani Oktaviyah, Dayanti Paisal, M. Pakaya, Renaldi Pamudji, Rama Adistya Nurcahya Pamuraharjo, Hemi Pangestu, Herwindu Danu Parwanti, Sri Pasai, Muda Wali Samudra Pekerti UA, Pekerti Permatasari, Armita Phebe Hendra Piarah, Wahyu Haryadi Prabowo, Arif Agung Prasetya, Dimas Prastiyo, Rian Pratama, Dinar Rezky Prihatin Oktivasari Probosuseno Probosuseno, Probosuseno Purwandari, Elce Purwanti, Nunik Purwojatmiko, B. Handoko Puspa, Eka Septariana Putra, Adhitya Nugraha Duwan Putra, Eriek Aristya Pradana Putra, Haris Maiza Putri, Depi Putri, Nadia Tiara Qodariyah, Niyar Ana R, Agam Bahtiar Rachel Turalely Rachmawati, Silvia Raharjo, Mugi Rahayu, Dewi Widiana Rahim, Abd Rahmadanti, Putri Rahmani, Mawardi Rahmani, Mawardi Rahmat Dani Rahmatulloh, Asep Rahmawati, Indriyani Dwi Rahmawati, Lilik Ratna Asmah Susidarti Ratna Asmah Susidarti Ratna Dewi Kusumaningtyas Refson Refson, Refson Reni Nurjasmi, Reni Retikasari, Frysca Virnanda Retno Murwanti Retno Wahyuningrum Rian Byhaqi Rias Gesang Kinanti Ridho Hans Gurning Ridlo, Zainur Rasyid Ridwan, Ahmad Hasan Ridwan, Vicky Bahru Rifky, Rifky Riyansyah Rizfie, Muhammad Dala Rofiq Sunaryanto Rohma, Adelia Nur Roni Mohammad Royhan, Royhan Rukmana, Andi Rulyansyah, Afib Ruslin Hadanu Rusmana, Najwa Rokhan Rustan Hatib S Mursiti S Siswanto Sabirin Matsjeh Saepul Nugraha Safarudin, Muhamad Sigid Safitri, Erica Meilia Said, Andika Pratama Said, Moch Imron Salati, Dianasanti Salim, Helmi Agus Samekto Wibowo Samekto Wibowo Samsul Arifin Saptono Saputri, Adinda Rizky Eka Saputri, Ginandjar Zukhruf Sari, Elisa Mardita Sari, Nurita Sari, Rosyidah Hikmah Sariman, Farid sarwo edy Satria Wati Pade Satrio Ardyantoro Sejati, Hiro Selirowangi, Nisaul Barokati Seto, Bayu Setyawan A, Setyawan Setyo Rini, Asih Sherin Ramadhania Shinta Ayu Lestari Shodiq, Achmad Shukatin, Shukatin Sidiq, Sidiq Simajuntak, Felizia Gabriella Sinensis, Rizki Amelia Sinta Suleman Siradjuddin Haluti, Siradjuddin Sirotjuddin, M. Siti Hamidah Siti Maghfirotun Amin Siti Maimunah Siti Musyrifah, Siti Siti Rahimah Siti Rodhiyah, Siti Siti Romlah Sitti Ayu Suhartina Yahya Sja'bani, Mochammad Soedjono Aswin Solih, Edwin Sahrial Sonny Taufan Sri Handayani Sri Hartatik Sri Haryanti Sri Juari Santosa Staddal, Iqrima Subaidi Subyanto, Subyanto Suherli, Ian Rakhmawan Sujono, Imam Sukanta, Deris Samba Pordi Sukari, Mohd. Aspollah Sukari, Mohd. Aspollah Sukidjo . Sulaeman Sumarta Sumbodo, Mugiatno Sunanto Sunanto Sunarti Sunarti Sunaryati Supendi, Dede Supriatini Supriatini Supriatini, Supriatini Supriyanto Supriyanto Surismiati Surismiati Suriyana Suriyana Suryani Suryani Susanti , Yuli Susi Iravati Susiana Candrawati Susy Yunita Prabawati Sutirman Sutirman Suwarman, Adika Suwita, Jaka Suyanto Suyanto Syamsul Ghufron Syarief, Ali TAUFIK ROHMAN, TAUFIK Tresna Dewi Tri Cahayati, Ulinnuha Triana Hertiani Triana Kusumaningsih Tsumu, Dhatu A Udi, Moh. Usni Putri, Saifa Utami, Satiti Vadlya, Vadlya Maarif Vero Ferdinand Putra Suseno VIKTARIA, Venansi Wahyono Wahyono Wahyuni, Fina Tri Warang, Japri Yusrifal Widya Asmara Wijaya, Rahman Benta Wina Libyawati, Wina Wisesa, Sindhu Wiwiek Fatchurohmah, Wiwiek Wiwik Cahyaningrum Woro Rukmi Pratiwi Wulandari, Friska Tri Wulansari, Rahmawati Yadi Janwari Yamin , Muhammad Yanti Dwi Astuti, Yanti Dwi Yayi Suryo Prabandari Yayuk Dwi Rahayu, Yayuk Dwi Yoga Prihastomo Yohandri Bow Yuli Asmi Rahman Yuliansah Yuliansah Yuniarti, Angger Styo Yushardi Yushardi, Yushardi Yusman, Nurjannah Zahroh, Fatimatus Zakiyah, Makhshushi Zikri Marifatullah Zuhratul Aini Mansur Zulaela Zulaela, Zulaela Zulkaisni, Zulkaisni Zuryati Djafar