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Hanny Hafiar

Program Studi Hubungan Masyarakat, Fakultas Ilmu Komunikasi, Universitas Padjadjaran

Author-ID : 272621

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Aktivitas ligninolitik Omphalina sp. hasil isolasi dari TKKS dan aplikasinya untuk dekolorisasi limbah kosmetik Ligninolytic activity of Omphalina sp. isolated from EFB and its application for decolorization of cosmetic waste . SUHARYANTO; Irma KRESNAWATY; Haryo Tejo PRAKOSO; Deden Dewantara ERIS
E-Journal Menara Perkebunan Vol 80, No 2: Desember 2012
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Abstract White-rot fungi (WRF) are belong to Basidiomycetes group that capable to degrade lignin, because they produce extracelullar ligninolytic enzymes such as lignin peroxsidase (Li-P), mangan peroxidase (Mn-P) and laccase. The ligninolytic activity can be used in bioprocess oxidation system such as biopulping, biobleaching and bioremediation. The purposes of this research were to determine the optimum conditions of growth and ligni-nolytic activity of Omphalina and to observe its potential to decolorize cosmetics wastewater. Omphalina sp. was grown on media of PDA-Remazol Brilliant Blue R (RBBR) and PDA-Guaiacol (GU) at various pH and temperature conditions. The decolorization of cosmetic effluent was conducted by applying Omphalina sp. at various dose of inoculum. Decolorization rate and change of COD were observed for eight days. The results showed that Ompha-lina sp. could grow and produce peroxidase enzyme both on RBBR and GU media at pH 4.5-8.5 and temperature 23-350C. Optimum dose of inoculum was as much as 5% w/v at which the fungus was able to decolorize cosmetic factory effluent up to 92.79% and to decrease COD value up to 48.57 % after eight days of incubation.Abstrak Jamur pelapuk putih (JPP) merupakan jamur kelompok Basidiomycetes yang mampu mendegradasi lignin karena memproduksi enzim-enzim ligninolitik ekstraseluler seperti lignin peroksidase (Li-P), mangan peroksidase (Mn-P) dan lakase. Kemampuan ligninolitik JPP dapat dimanfaatkan dalam sistem oksidasi bioproses seperti biopulping, biobleaching dan bioremediasi. Pene-litian bertujuan menetapkan kondisi optimum pertumbuhan Omphalina sp. dan aktivitas ligninolitik yang dihasilkan-nya serta mempelajari potensinya dalam mendekolorisasi limbah cair kosmetik. Omphalina sp. ditumbuhkan dalam media PDA-Remazol Brilliant Blue R (RBBR) dan PDA-Guaiakol (GU) pada berbagai variasi pH dan suhu. Percobaan dekolorisasi limbah cair kosmetik dilakukan dengan aplikasi inokulum dalam berbagai dosis. Laju dekolorisasi dan perubahan COD diamati selama delapan hari. Hasil penelitian menunjukkan Omphalina sp. tumbuh dan menghasilkan enzim peroksidase, baik pada media RBBR maupun GU pada pH 4,5-8,5 dan suhu 25-350C. Dosis optimum aplikasi Omphalina sp. adalah 5% (b/v) yang mampu mendekolorisasi limbah cair pabrik kosmetik hingga 92,79% dan menurunkan COD 48,57% setelah delapan hari.

Sintesis reagen imunokimia untuk deteksi okratoksin dengan metode imunokromatografik nanopartikel emas Synthesis of immunochemical reagent for ochratoxin detection using gold nanoparticle immunochromatographic method Irma KRESNAWATY; Romsyah MARYAM; . SUHARYANTO; Sumi HUDIYONO
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Abstract The quality of Indonesiam coffee andcocoa products has been declined due to the contamination of fungi producing ochratoxin, a serious human mycotoxin. Therefore, develop-ment of fast, accurate and simple method for early detection of ochratoxin contamination is required. As a part of research attempted to develop early detection technique of ochratoxin in severall commodities, especially agricultural products, the objective of this study was to produce immunochemical reagent for ochratoxin detectionusing immunoglobulin Y (IgY) based on immunochromatographic method. Results showed that OTA-OVA could be synthesized using active ester method with addition of N-hydroxy-succiimide and dicyclocarboimide. The inter-mediate compound produced showed C=O stretching vibrational band at 1600 cm-1 and C-O stretching vibrational band at 1300-1000 cm-1. Antibody-gold nanoparticle conjugate was optimally produced at pH 9 and with antibody dilution of 1:7.5 (v/v). There was 50 nm absorb-tion shift in visible absorbtion after the antibody was conjugated with gold nanoparticle. Even though the test strip did not show clear visual-ization, the cut off of ochratoxin concentrationis obviously determined at 10 ppb. This results suggest thatthe technique could be used to detect ochratoxin contamination. Abstrak Komoditas kopi dan kakao Indonesia ter-kendala masalah mutu produk yang rendah akibat kontaminasi jamur yang menghasilkan okra-toksin. Okratoksin merupakan mikotoksin yang membahayakan kesehatan manusia. Oleh karena itu, cara deteksi dini kontaminasi okratoksin yang cepat, akurat dan mudah perlu dikembangkan. Sebagai bagian dari usaha untuk mengembangkan teknik deteksi dini okratoksin pada pada berbagai komoditas, khususnya produk pertanian, pene-litian ini bertujuan menghasilkan reagen imuno-kimia yang menggunakan antibodi immune-globulin Y (IgY) berdasarkan metode imunokro-matografik untuk deteksi okratoksin. Hasil penelitian menunjukkan bahwa OTA-OVA dapat disintesis dengan metode ester aktif dengan menambahkan N-hidroksisuksiimida dan disiklo-karboimida. Senyawa antara yang dihasilkan memiliki absorpsi pada frekuensi 1600 cm-1 yang menunjukkan adanya vibrasi ulur ikatan C=O dan adanya banyak absorpsi pada 1300-1000 cm-1 yang mengindikasikan adanya serapan ulur yang kuat ikatan C-O. Konjugat antibodi-nanopartikel emas dihasilkan optimum pada pH 9 dan dengan pengenceran antibodi 1:7,5 (v/v). Hasil pengujian spektrofotometer visible menunjukkan adanya pergeseran serapan sebesar 50 nm setelah anti-bodi dikonjugasikan pada nanopartikel emas. Meskipun secara visual tidak begitu jelas, tetapi hasil pengujian pada teststrip menunjukkan bahwa nilai cut off konsentrasi okratoksin yang terdeteksi adalah10 ppb. Hasil ini menunjukkan bahwa teknik yang dikembangkan dapat diguna-kan untuk deteksi kontaminasi okratoksin.

Lipase spesifik 1,3-gliserida dari fungi lokal untuk biokonversi CPO menjadi diasilgliserol Specific lipase of 1,3-glyceride from indigenous fungi for bioconversion of CPO to produce diacylglycerol . TRI-PANJI; . SUHARYANTO; Nining ARINI
E-Journal Menara Perkebunan Vol 76, No 1: Juni 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

SummaryDownstream industry of palm oil producing specialty oil with higher economic value compared to that of CPO in Indonesia is less developed due to technical obstacle and the availability of supporting materials. Specific lipase 1,3-glyceride for example which is used for oleochemical processing of healthy oil production is still imported with relatively high price. Healthy oil can be made from CPO bioconversion using the enzyme that produces oil rich in diacylglycerol (DAG). Although research on the production and the use of lipase has been well studied, production of specific lipase from microbes of local source is still very limited. This article reports one part of the series of the research activities on bioprocess and genetic engineering approaches to produce specific lipase for bioconversion of CPO i.e optimization of 1,3-glyceride-spesific lipase production from fungi selected from local sources. Based on the fluorescence zone on the screening media, of the twenty isolates collection, it was found that P6 isolate, thereafter indentified as Neurospora sitophila, has the highest activity of 1,3-glyceride-specific lipase. The lipase of N. sitophila was able to catalyze glycerolysis of triacylglycerol (TAG) in CPO to produce DAG. The bioconversion products of lipase yielding ratio of DAG/TAG was higher than ratio of free fatty acids (FFA)/TAG (0.12 > 0.08). The optimum condition of the enzymatic bioconversion was at 40 oC, pH 6, and 10-day incubation. The primary fatty acids on the DAG were oleic (56.2%), palmitic (40.0%), and myristic (2.7%) acids. The decrease of palmitic acid on DAG compared to on TAG, indicated that the lipase of N. sitophila worked relatively specific at C1 or C3 of the TAG.Kurang berkembangnya industri hilir yang menghasilkan minyak khusus yang nilainya berlipat dibandingkan CPO antara lain karena hambatan teknis dan ketersediaan bahan pendukungnya. Lipase spesifik 1,3-gliserida misalnya, yang digunakan untuk produksi minyak kesehatan, masih diimpor dengan harga relatif tinggi. Minyak kesehatan dapat diproduksi dari biokonversi CPO dengan lipase spesifik 1,3-gliserida hingga diperoleh minyak yang kaya kandungan diasilgliserol (DAG). Tulisan ini melaporkan optimasi aktivitas lipase spesifik 1,3-gliserida dari fungi isolat lokal terpilih. Berdasarkan zona fluoresens pada medium penapis lipase, dari 20 isolat fungi yang diuji isolat P6 yang kemudian diidentifikasi sebagai Neurospora sitophila memiliki aktivitas tertinggi dan bersifat spesifik 1,3-gliserida. Lipase N. sitophilamampu mengkatalisis gliserolisis triasilgliserol (TAG) dalam CPO untuk menghasilkan DAG. Lipase tersebut menghasilkan nilai perban-dingan DAG/TAG lebih besar dari nilai perbandingan asam lemak bebas (ALB)/TAG (0,12 > 0,08). Kondisi optimum biokonversi enzimatis ini terjadi pada suhu 40 oC, pH 6, dan waktu inkubasi selama 10 hari. Asam lemak utama penyusun DAG adalah asam oleat (56,2%), palmitat (40,0%), dan miristat (2,7%). Berkurangnya asam palmitat pada DAG dibanding pada TAG menunjukkan bahwa lipase N. sitophila bekerja secara relatif spesifik pada C1 atau C3 dari gliserida.

Biokonversi minyak sawit kasar menggunakan desaturase amobil sistem curah pada skala semipilot Bioconversion of crude palm oil using immobilized desaturase in batch system at semi pilot scale . TRI-PANJI; . SUHARYANTO; . GUNAWAN; Khaswar SYAMSU
E-Journal Menara Perkebunan Vol 73, No 2: Desember 2005
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

SummaryIncreasing unsaturation level of crude oilpalm (CPO) could be carried out by usingdesaturase enzyme of Absidia corymbifera. Thisbiocatalyst could also produce polyunsaturatedfatty acids (PUFA) such as gamma linolenic acidthat beneficial for healthy oil. The objective ofthis research was to determine the optimumcontact time and ratio of immobilized desaturaseenzyme-substrate in batch system at semi pilotscale (5,000-15,000 mL). Desaturase wasextracted from A. corymbifera biomass andimmobilized on activated zeolite (3-6 mm).Immobilized enzymes were then used forbioconversion process in batch system by mixingthe enzyme with CPO in a bottle placedhorizontally then rotated using a rotator machineat room temperature (25-30 o C). The resultshowed that optimum contact time with ratioimmobilized enzyme-substrate 1:1; 1:2; and 1:3were 30, 40, and 50 min resulted in increasingiodine number 2.84; 3.94; and 4.46 g I 2 /100 gCPO, respectively. An optimum enzyme-subtrateratio was achieved at 1:2, resulted in increasingof iodine number 9-11 g I 2 /100 g CPO, productrecovery of 17,000 mL (21 batches) up to 18 hours. It was detected that active desaturasesduring CPO bioconversion were  6 ,  9 , and  12 desaturases as shown by the increase of oleic(4.5%), linoleic (0,85%) and linolenic acids(60.7%).RingkasanPeningkatan ketidakjenuhan minyak sawitkasar (crude palm oil, CPO) dapat dilakukandengan enzim desaturase Absidia corymbifera.Biokatalis ini juga mampu menghasilkan asamlemak tidak jenuh majemuk (polyunsaturatedfatty acids, PUFA) yang bermanfaat untukkesehatan seperti asam gamma linolenat (GLA).Tujuan penelitian adalah menetapkan waktukontak dan nisbah enzim desaturase amobil-substrat optimum dalam sistem curah pada skalasemipilot (5.000-15.000 mL). Desaturase di-ekstraksi dari biomassa A. corymbifera dandiamobilisasi pada zeolite (3-6 mm) yang telahdiaktivasi. Enzim amobil kemudian digunakanuntuk proses biokonversi dalam sistem curahdengan cara mencampurkan dengan CPO dalambotol yang diletakkan secara horizontal kemudiandiputar dengan mesin rotator pada suhu ruang(25-30 o C). Hasil penelitian menunjukkan bahwawaktu kontak optimum enzim desaturase-substratdengan nisbah 1:1; 1:2; dan 1:3 adalah 30, 40,dan 50 menit dan menghasilkan peningkatanbilangan iod berturut-turut sebesar 2,84; 3,94;dan 4,46 g I 2 /100 g CPO. Nisbah enzim-substratoptimum dalam proses biokonversi CPO adalah1:2 yang menghasilkan peningkatan bilangan iod9-11 g I 2 /100 g CPO dan perolehan produk17.000 mL (21 kali curah) selama 18 jampemakaian. Penelitian juga dapat mendeteksibahwa desaturase yang aktif selama prosesbiokonversi CPO adalah  6 ,  9 , dan  12desaturase yang ditunjukkan oleh peningkatanasam oleat (4,5%), linoleat (0,85%) dan linolenat(60,7%).

Produksi imunoglobulin Y (IgY) untuk pengembangan metode deteksi dini kontaminasi okratoksin (Immunoglobulin Y (IgY) production to develop an early detection method for ochratoxin contamination) Irma KRESNAWATY; . SUHARYANTO; . SISWANTO; Sumi HUDIYONO
E-Journal Menara Perkebunan Vol 86, No 1 (2018): April, 2018
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v1i1.279

Indonesian coffee and cocoa commodities are constrained by low product quality problem due to contamination of fungal metabolites which producing ochratoxin A (OTA). Ochratoxin is neprotoxic, immunogenic, carcinogenic and teratogenic to the human health. Early detection method on site detection should be developed because of those negative effects. The aim of this study was to produce antibody to develop a method for OTA detection. Antibody was produced by immunization of egg laying hen. Antibody-produced was sepatared and analyzed using ELISA (Enzyme-Linked Immunosorbent Assay) and DBIA (dot blot immunoassay),and tested its composition using HPLC and SDS PAGE. The results showed that anti-OTA polyclonal antibodies had been obtained already from chicken eggs in the 4th period (7 weeks after initial immunization). These antibodies showed anti-OTA reactivity by DBIA method and still showed anti-OTA reactivity up to 9th period (12 weeks after initial immunization). The anti-BSA antibodies produced should be removed to increase the sensitivity of antibodies againts ochratoxin A. The separation of BSA antibodies can be conducted by the absorption of the protein. [Keywords: ochratoxin A; early detection; antibody IgY]. AbstrakKomoditas kopi dan kakao Indonesia terkendala masalah mutu produk yang rendah akibat kontaminasi cendawan penghasil okratoksin A. Okratoksin A (OTA) bersifat neprotoksik, imunogenik, karsinogenik dan teratogenik yang membahayakan kesehatan manusia. Karena efek negatif yang diakibatkan oleh mikotoksin ini, maka perlu dikembangkan deteksi dini kontaminasi okratoksin langsung di lokasi. Penelitian ini bertujuan menghasilkan antibodi imunoglobulin Y (IgY) untuk mengembangkan metode perakitan perangkat deteksi cepat berbasis imunologi untuk deteksi OTA. Antibodi dihasilkan menggunakan uji ayam petelur. Antibodi yang dihasilkan dipisahkan dan dianalisis aktivitasnya dengan ELISA (Enzyme-Linked Immunosorbent Assay) dan DBIA (dot blot immunoassay), serta diuji komposisinya dengan HPLC dan SDS PAGE. Hasil penelitian menunjukkan bahwa antibodi poliklonal anti-OTA sudah diperoleh dari telur ayam pada periode ke-4 (7 minggu setelah imunisasi awal). Antibodi ini menunjukkan reaktivitas anti-OTA dengan metode DBIA dan masih menunjukkan reaktivitas anti-OTA sampai periode 9 (12 minggu setelah imunisasi awal). Komposisi asam amino antibodi anti-OTA menunjukkan perbedaan dengan komposisi asam amino IgY di database. Antibodi anti BSA yang dihasilkan harus dihilangkan terlebih dahulu untuk meningkatkan sensitivitas antibodi terhadap okratoksin A dan pemisahan dapat dilakukan dengan penyerapan antibodi BSA.[Kata Kunci: okratoksin A; deteksi dini; antibodi IgY].

Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase . SISWANTO; . SUHARYANTO; Rossy FITRIA
E-Journal Menara Perkebunan Vol 75, No 2: Desember 2007
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

ANALISIS PENGARUH PERTUMBUHAN EKONOMI, PENDUDUK MISKIN DAN UPAH MINIMUM TERHADAP INDEKS PEMBANGUNAN MANUSIA DI KABUPATEN BUNGO 2014-2021 Lina Marliana
Eqien - Jurnal Ekonomi dan Bisnis Vol 10 No 2 (2022): EQIEN - JURNAL EKONOMI DAN BISNIS
Publisher : Sekolah Tinggi Ilmu Ekonomi Dr Kh Ez Mutaqien

Indicators of socio-economic development have long been a matter of debate. Economists and non-economic groups have challenged the question of per capita income as an indicator of development because of the inaccuracy of these indicators, so several new indicators have emerged. The new indicators generally focus on human development. This study aims to describe the influence of independent variables such as the number of poor people, economic growth and the minimum wage on the Human Development Index in Bungo. The research method used is multiple linear regression analysis and processed using the SPSS application. Based on the t-test, have been found that economic growth and workers' minimum wage had a significant effect on the Human Development Index. In contrast, the number of poor people had no significant effect on the Human Development Index. The regression model in this study is the Best Linear Unbiased Estimator (BLUE). This research is expected to be used as input for human development planning in Bungo Regency.

Produksi Spirulina platensis dalam fotobioreaktor kontinyu menggunakan media limbah cair pabrik kelapa sawit Production of Spirulina platensis in continous photobioreactor using palm oil mill effluent media . SUHARYANTO; . TRI-PANJI; Shinta PERMATASARI; Khaswar SYAMSU
E-Journal Menara Perkebunan Vol 82, No 1: Juni 2014
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractCultivation of Spirulina platensis in an abundant available and inexpensive medium such as palm oil mill effluent (POME) will produce biomass and valuable active materials at competitive price. Utilization of POME will also reduce pollution level and support cleaned production. The objectives of this research were to determine the dilution rate of S. platensis and the reduction rate of pollution level of POME on continuous photobioreactor. Preliminary research was conducted by growing S. platensis on POME medium with various concentration, namely 25%, 50%, 75%, and 90% POME on batch system. The experiment was conducted in 1.2 L capacity continous photobioreactor using medium containing a mixture of POME and synthetic medium. Feeding rate was set up at 0.05 mL/5 sec. (dilution rate of 0.03 hr -1), 0,05 mL/10 sec. (dilution rate of 0.015 hr -1), and 0.05 mL/15 sec. (dilution rate of 0.01 hr -1). For optimum dilution rate, the experiment was scaled up eight times using 10 L capacity continous photobioreactor. The results showed that optimum growth rate of S. platensis (µmax) = 0.233, was achieved using medium consisting of 90% POME and 10% synthetic medium after two weeks. Dilution rate of 0.015 hr -1 on photobioreactor was the optimum dilution rate for growth of S. platensis as well as for decreasing polution level of POME. The result of the eight-times scale up photobioreactor using flow rate of 0.4 mL/10 sec and dilution rate of 0.015 hr -1 showed that the growth of S. platensis was relatively constant as reflected by the OD value of the suspension culture and the concentration of cellular biomass. At the optimum condition, production of S. platensis biomass was 0.267g/L and pollution level was decrease 24%. The rate of outflow also resulted the constant decrease of polution level based on total carbon (TC), total dissolve solid (TDS), dissolve oxygen (DO), BOD, and COD parameters indicating that continuous photobioreactor was running at steady state.Abstrak Kultivasi S. platensis dalam media yang tersedia me-limpah dan murah seperti limbah cair pabrik kelapa sawit (LC-PKS) akan menghasilkan biomassa dan bahan aktif bernilai ekonomi tinggi dengan harga kompetitif. Pemanfaatan LC-PKS juga akan mengurangi dampak pen-cemaran lingkungan dan membantu menciptakan sistem produksi bersih. Penelitian ini bertujuan menetapkan laju dilusi optimum per-tumbuhan S. platensis dan laju penurunan tingkat cemaran LC-PKS pada fotobioreaktor sistem kontinyu. Untuk mengukur laju alir sistem kontinyu, pertama S. platensis ditumbuhkan pada media LC-PKS 25%, 50%, 75%, dan 90% dengan sistem batch. Pertum-buhan S. platensis pada fotobioreaktor sistem kontinyu kapasitas 1,2 L dirancang dengan variasi laju alir umpan berupa LC-PKS yang dicampur media sintetik pada konsentrasi optimum. Variasi laju alir pengumpanan diatur pada variasi 0.05 mL/5 detik (laju dilusi 0,03 jam-1), 0,05 mL /10 detik (laju dilusi 0,015 jam -1), dan 0,05 mL/15 detik (laju dilusi 0,01 jam-1). Pada laju alir optimum, skala percobaan diperbesar delapan kali menggunakan foto-bioreaktor berkapasitas 10 L. Hasil penelitian menunjukkan bahwa laju pertumbuhan maksimum (µ maks) adalah 0,233 jam-1 yang diperoleh dengan campuran media LC-PKS 90% dan media sintetik 10% selama dua minggu. Fotobioreaktor dengan laju dilusi 0,015 jam-1 merupakan laju alir umpan yang optimum untuk pertumbuhan S. platensis serta menghasilkan penurunan tingkat cemaran LC-PKS yang optimum. Hasil penelitian dengan perbesaran skala delapan kali menggunakan laju alir pengumpan 0,4 mL/10 detik (laju dilusi 0,015 jam-1) menunjukkan bahwa pertumbuhan S. platensis relatif konstan. Produksi biomassa sel rata-rata sebesar 0,267g/L dan kadar cemaran limbah rata-rata menurun sebesar 24%. Laju alir keluar (outflow) juga menghasilkan kadar cemaran limbah yang konstan ber-dasarkan parameter total karbon (TC), total dissolve solid (TDS), dissolve oxygen (DO), BOD, dan COD yang menunjukkan bahwa sistem fotobioreaktor kontinyu ini berjalan dengan baik.

Biosorpsi ion tembaga dalam limbah tailing menggunakan jamur pelapuk putih amobil Biosorption of copper ion in tailing mining effluent using immobilizede white-rot fungi Firda DIMAWARNITA; . SUHARYANTO; . TRI-PANJI1; Nur RICHANA; Achmad ZAINUDIN
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Abstract Copper and gold mining industry produce large amount of tailings effluent containing heavy metals such as Cu and Hg that will polute environment and agricultural land, if it is not properly treated. Effort in reducing pollution level and recovery of metal in tailing could be conducted with biosorption process using microbial cell as an absorbent agent. This study aims to determine the ability of selected white rot fungi (WRF) immobilized on empty fruit bunches of oil palm (EFB) to absorb Cu (II) metals in tailing. Based on growth rate, ligninolytic enzymes activity, and the absorption capacity of heavy metals, the superior WRF candidate was Omphalina sp. The ability of biomass Omphalina sp. to decrease the con-centrateion of Cu (II) with an initial concentration of 150 ppm, up to 63%. Omphalina sp. that immobilized on TKKS was able to reduce Cu (II) as much as 66-77% at pH 4.0 for 60 minutes. Abstrak Eksploitasi tambang tembaga dan emas banyak menghasilkan limbah tailing yang masih me-ngandung logam berat seperti Cu (II) dan Hg (II). Limbah tersebut berpotensi mencemari perairan dan lahan pertanian bila tidak ditangani dengan baik. Usaha untuk mengatasi limbah tailing dan sekaligus memekatkan (recovery) logam di dalam-nya dapat dilakukan dengan proses biosorpsi menggunakan sel mikroba. Penelitian ini bertujuan menetapkan kemampuan biomassa jamur pelapuk putih (JPP) yang diimobilisasi pada tandan kosong kelapa sawit (TKKS) dalam mengabsorpsi ion logam Cu (II). Metode biosorpsi logam yang digunakan dengan sistem batch dengan kadar logam 300 ppm, 200 ppm,dan 150 ppm. Hasil seleksi JPP berdasarkan laju pertumbuhan, aktivitas enzim ligninolitik, dan penyerapan logam berat telah diperoleh kandidat JPP unggul yaitu Omphalina sp. Dalam media PDB, Omphalina sp. toleran terhadap Cu (II) hingga konsentrasi 150 ppm. Kemampuan biomassa Omphalina sp. untuk penurunan konsentrasi Cu (II) dengan konsentrasi awal 150 ppm yaitu mencapai 63%. Omphalina sp. yang diimobilisasi dengan TKKS mampu menurun- kan kandungan Cu (II) sebesar 66-77% pada pH 4.0 selama 60 menit.

Optimasi produksi diasilgliserol dari crude palm oil menggunakan lipase spesifik 1,3-gliserida dari Rhizopus oryzae TP-2 Optimation of diacylglycerol production from crude palm oil using specific lipase of 1,3-glyceride from Rhizopus oryzae TP-2 . SUHARYANTO; . TRI-PANJI; Urip PERWITASARI
E-Journal Menara Perkebunan Vol 79, No 1: Juni 2011
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

AbstractModern lifestyle caused the increasing prevalence ofobesity that precedes degenerative disease such as coronarycardiovascular disease, hypercholesterolemia, stroke, anddiabetes mellitus. Use of healthy oil in the daily food diet couldreduce the risk of the disease. One of healthy oils that proved tobe useful for human health is diacylglycerol (DAG).Unfortunately, production of DAG in Indonesia is hampered bythe relatively high price of the lipase enzyme. To overcome theprovision of costly imported lipase in producing DAG, aresearch was conducted by employing crude extract of lipaseenzyme from an indigenous mold namely Rhizopus oryzaeTP-2. Crude extract of lipase enzyme from mycelium culturefiltrate was freeze dryed and used for crude palm oil (CPO)bioconversion through glycerolysis at various processcondition. The objective of this research was to determineoptimum variable of temperature, incubation time, amount ofsubstrate and pH in producing DAG from CPO using lipase ofR. oryzae TP-2. The reseach result showed that lipase fromR. oryzae TP-2 was proved to be specific at position of 1,3-glyceride as indicated by glycerolysis products i.e DAG/TAGratio 0.48 higher than that of FFA/TAG ratio 0.06. Optimumconditions for glycerolysis were at temperature 37 o C, pH 7,3 g of CPO substrate, and 18 hours of incubation time. DAGyield by this optimum condition reach as much as 20.76 % w/w.The lipase derived from this experiment produced DAG betterthan that of using imported commercially lipase enzyme ofRhizomucor meihei.AbstrakGaya hidup modern telah menyebabkan meningkatnyakasus kegemukan yang berdampak timbulnya berbagaipenyakit degeneratif seperti jantung koroner, hiper-kolesterolemia, stroke dan diabetes mellitus. Penggunaanminyak sehat (healthy oil) sebagai menu diet sehari-hari dapatmengurangi faktor risiko penyakit tersebut. Salah satu jenisminyak sehat yang terbukti berdampak positif pada kesehatanmanusia adalah diasilgliserol (DAG). Sayangnya, produksiDAG di Indonesia terkendala oleh mahalnya enzim lipasespesifik 1,3-gliserida yang masih harus diimpor. Untukmengatasi mahalnya enzim lipase impor dalam produksi DAGdari CPO, penelitian penggunaan ekstrak kasar lipase darikapang lokal Rhizopus oryzae TP-2 telah dilakukan. Ekstrakkasar lipase dari filtrat kultur miselium R. oryzae TP-2dikeringbekukan dan digunakan untuk biokonversi CPOmelalui proses gliserolisis pada berbagai kondisi reaksi.Penelitian bertujuan menetapkan peubah suhu, waktu, jumlahsubstrat dan pH optimum dalam produksi DAG menggunakanlipase dari R. oryzae TP-2. Hasil penelitian menunjukkanbahwa enzim lipase R. oryzae TP-2 bersifat spesifik1,3-gliserida yang ditunjukkan oleh nisbah DAG/TAG, yaitu0,48 lebih besar dari nisbah ALB/TAG yaitu sebesar 0,06.Kondisi optimum untuk gliserolisis CPO adalah waktu inkubasiselama 18 jam, suhu reaksi 37°C, jumlah substrat CPO 3 g, danpH reaksi 7. Hasil DAG pada kondisi optimum gliserolisisadalah 20,76 %. (b/b). Lipase R. oryzae TP-2 yang digunakandalam penelitian ini menghasilkan DAG lebih tinggi dari padalipase impor asal Rhizomucor meihei.

Co-Authors . Gunawan . Gunawan . SISWANTO A H SARAGIH A Halim A.A. Ketut Agung Cahyawan W Aat Ruchiat Nugraha Abdul Hamid Abdul Muis Abimanyu, Ade Anom Acep Purnama, Acep Achmad Jazuli Achmad Noor Fatirul Achmad Zainudin Adawiyah, Jihan Rizki Ade Kadarisman Adelina Hasyim Adi Prianto, Adi Aditya K. Karim Adzkia Kirana Dipa Adzkia Kirana Dipa Afra Mulya Alamsyah Agil Arif Nugraha, Agil Arif Agus Rahmat agus rahmat Agustina, Kristia Ahamed, Forbis Ahmad Fudholi Aini, Nurulitaâ€™ Aisyah, Novrisa Nu Akbar, Thoriq Nul Albina Tikupasang Alfian Romadhoni Alfiolita Widya Safitri Ali Djamhuri Ali Fahmi Allegra Gabriella Esther Almira, Sherica Rafa Alyssa Rasheedah Amalia Raharjo, Chiquitita Amaliah, St. Mukhlisahtul Amanda Putri, Tasha Suciati Amar Shiddiq Junaidi Amida Windari Fitria Amirul Fatihin, Amirul Amruddin Amruddin Andi W, Andi Andika Pakaradena Andreas Febrianto, Andreas Anggita Stovia Anisa L.K. Anisti, Anisti Anna Muawanah Annisa Rahmawati Annisa Yutha Anton Irawan Anuraga Jayanegara Anwar Sani Anwar, Adani Nurshadrina Aprilia, Adinda Putri Aprilliyani, Ega Aqila, Amyra Amalia Ari Agung Prastowo Ari Agung Prastowo, Ari Agung Ari Andriyani Arief Cholil Arief Syaichu Rohman, Arief Syaichu Arien Afriesta Aries Buana Arif Sofianto, Arif Arif, M. Ichwan Arifin Muslim Armilia Sari, Armilia Arsyafira, Nabilla Arya, Nyoman Ngurah Arya, Nyoman Ngurah Aryanti, Della Aryanti, Della Ashari, Muhammad Syahwal Asjari, Fachrudy Asmin Lubis Asmini Budiani Asmorowati, Rica Susy Astrid Eveline Athiutama, Ari Atwar Bajari Aulia, Karina Ayu Puspa Yurita Azlin Atika Putri Bachri, Aep Saeful Bahraeni Lajamani Bahrun Ali Murtopo Baiq Vira Safitri Bambang Riadi Bambang Suharno Barezki, Fisti Eliana Barlian, Tria Dara Bayu Arga Krisna Bella, arkita Vierza Benny Ranti Benny Ranti beny firman, beny Bertha Silvia Pratiwi, Bertha Silvia Bima Anjasmoro, Bima Binantoro, Patria Winahyu Brata, Muhammad Ibrahim Briliani Arfidhiya, Najmiya Budi Santosa Budi Santosa Budiana, Hery Bustanul Arifin C. Priyatna, Centurion Calista, Nadia Carolus Borromeus Rasrendra Centurion C. Priyatna Centurion C. Priyatna Centurion Chandratama Priyatna Centurion Chandratama Priyatna, Centurion Chandratama Centurion Chendy Priyatna Chairil Anwar Chandratama Priyatna, Centurion Chris D. Sembay, Chris D. Cindy Lidya Cipta Endyana Citra, Laras Wibawati Dadan Rohdiana Dadang H, Dadang Dadang Sugiana Dadiyono Amat Pawiro, Dadiyono Dais Iswanto Dally Rahman Damanhuri Daud Dandi Supriadi Darmawan Setiya Budi Darmawan, Jadmiko Darmawan, Jadmiko Darsono Darsono Dasrun Hidayat Dasrun Hidayat Davi Sofyan Deddy Mulyana Deden Dewantara ERIS Desi Febrianti Destina Kasriyati Desyta Ulfiana Detta Rahmawan Detta Rahmawan, Detta Detya Wiryany Devi Risma DEWI APRI ASTUTI Diah Fatma Sjoraida Diah Fatma Sjoraida Diah Sri Rejeki Dian Wardiana Sjuchro Diana Hardiny Diana Puspitasari Dianto, Ryan Dien Nur Chotimah Dimas Fahreza Purba DINI KURNIAWATI Dipa, Adzkia Kirana Diyono, Diyono Djabir Hamzah Djoko Legono Djoko Santoso Djufri - Doti Wahyuningsih Dwi Desilvani Dwi Masrina Dwi Ridho Aulianto Dwidjono Hadi Darwanto Edwin Rizal Edy Murdani Z Effendi, Mas'ud Efrida Efrida Efrin Umma Nassaluka Eka Abdi Eka Firmansyah Eka Widiyananto, Eka Eko Kurniawan Eko Kurniawan Eko Purnomo Eli Amaliyah Ely Triasih Rahayu Endang Uliyanti Engkus Kuswarno Erna Lestari Erna Umar Ernawati, Agustin Ertambang Nahartyo Erungan, Delano E. H. Ervina Indrayani Eryta Deka Wulan Agustin Ety Aryati, Ety Etyn Yunita Evendi Evendi Evi Adriani Evi Novianti Evi Novianti Evi Novianti Evi Tahapari, Evi Fadrul Fadrul Fajar Syuderajat Fajrianthi Fajrianthi Fakhri Zakaria Farhan Suherman Fasya Al Rahmah Fasya Maudia Fathia Iqbal, Najla Fathiya Nur Rahmi Fathullah . Fatimah Karim Fatma S, Diah Fauzi Febrianto Fauzia Aristalindra Fauzy Akmal Muslimin Febriansyah, Erwin Febrianti Asbaningsih, Febrianti Febrianti, Rita Febrianti, Tantri Fedi Faizar Pratama Feliza Zubair Feliza Zubair Filza Intan Mariezka Firda DIMAWARNITA Firda Firdaus Abdi Firsoni Firsoni, Firsoni Fisti Eliana Barezki Fithriyah Patriotika FX Ari Agung P FX. Ari Agung Prastowo Giani Levina Gilang Dwi Prasetiyo Gilang Gumelar Gita Dwi Karisma Guesa Maiwinda Gusdiansyah, Edo Hadiyana Sukandar, Hadiyana Hakim, Aji Zul Hakim, Rahayu Marini Hana Budi Prativi, Hana Budi Handoyo, Sri Hanifah, Karina Putri Hanna Herfina Hanna Herfina, Hanna HAPPY WIDIASTUTI Harahap, Abdul Anas Hardani, Shintia Nugrahini Wahyu Hardiansyah Hardianysah Hartati Hartati Hartati Hartati Hartati Soetjipto Haryo Tejo PRAKOSO Haryono Haryono Haryono Haryono Haryono, T. Hasnudi, Hasnudi Hecka Athaya Shabrina Helman Helman, Helman Heri Abijono Heri Widodo Herke Regitadika Herlina Agustin Heru Riyanto Budiana Heru Riyanto Budiana Heru Riyanto Budiana Heru Ryanto Heru Ryanto Budiana Hijriyatun Hijriyatun Hikmatud - Daroini, Hikmatud - Hilmi, Indah L. Hirsanuddin Hirsanuddin Husnah Husnah I Gde Adnyana Sudibya I Gede Riana I Gusti Agung Gede Witarsana I Gusti Agung Sri Rwa Jayantini I Gusti Ayu Agung Dian Susanthi I Gusti Gede Anggareksa Putra Mulyawan I Gusti Ngurah Agung Suprastayasa I Ketut Artawa I Komang Gede Wiryawan I Made Sumarya I Nyoman Kardana I Putu Very Ferrari I Wayan Sukma Winarya Prabawa Ida Ayu Made Wedasuwari Ida Bagus Putra Yadnya Idah Hamidah Idah Hamidah Ilfa Khairina Ilham Gemiharto Ilhamsyah Ilhamsyah Ima Fitha Patasik, Ima Fitha Imas Maesaroh Indra Gunawan Iqbal Ghani Morotjono Ira Mirawati Irawan Sugoro Irawaty, Adissa Irfan Hidayat Irhami Irhami Iriana Bakti Irma Badarina Irma KRESNAWATY Irma Kresnawaty Irma Suryani Irwan Akib irwan meilano Is Mardianto, Is Ismail Suardi Wekke Ismail, Nurzali Isni Raihan Khalish Isri R. Mangangka, Isri R. Istiqamah Istiqamah Ita Fitriana Ivanna Kristianti Timotius Iwan Setiawan Izatturrahman, M. Hifzhan Izhary Siregar Jamhari Jamhari Jangkung Handoyo Mulyo Jasmine Putri Syadhana Jatmiko, Eko Jauhari Jauhari Jaya, Raymond Clement Jayawarsa, A.A. Ketut Jenny Ratna Suminar Jenny Rizkiana Jessica Wandita Putri Jimi Narotama Mahameruaji Joko Pramono Joyo Winoto, Joyo Juhaeri’ah Juhaeri’ah Junaidi Sagir Kamaluddin Kasim Kandi, Nabila Raihan Khairiyah, Diah Khairunnisa, Silka Khalida Azki Maudina Khaswar Syamsu Kholidil Amin Kholil Kholil Kokom Komariah Komang Astiari Komang Gde Adikresna D Krisma Widi Wardani Kristian Adi, Kristian Kuntayuni Kuntayuni Kuraesin, Penti Dewi Kuraesin, Penti Dewi Kurnia Dwi Sari Utami Kurnia Dwi Tilasanti Kurniawan, Agnikafitriya Kusuma Nata, Agus Wisnu Lahade, Ruth Anggraeni Putri Lailatin Nuriyah Laily Fitriani Laksono Trisnantoro Latsmira, Lila Leni Marliani Lestari, Dini Tri Lestari, Puji Levana, Azka Putri Lifia, Yoli Okta Lili Fajria Lilik Linawati Lilis Puspitasari Limilia, Putri Linawaty Linawaty Lisiard Dimara Liszulfah Roza Lukiati Komala Lukita Komala Erdinaya Lukmandono Lydia Ninan Lestario M Irfani ABDULLAH M, Sri Mega M. Fakhruriza Pradana, M. Fakhruriza M. Kamarea M. Sood M. Sood M.Pd S.T. S.Pd. I Gde Wawan Sudatha . M.Pd. Dra. Made Sulastri . Madania Fariha Shifa Made Darmiati Made Sri Satyawati Maemonah, Maemonah Maesaroh, Imas Magrifatul Zania Maharani MAHAMERUAJI, JIMI N. Mahaputra, I Ketut MAHYUMI RANTINA, MAHYUMI Maikel Tuturop Mailangkay, Adele B. L. Manuputty, Audrey MARATUS SHOLIHAH Marindra Firmansyah Mariyatni, Ni Putu Sri Marvian, Ananda Marwaningsih, Veronika Ririn Mastar Asran Maudy Rizkiana Poedjadi mawadi, mawardi Mazaya, Witiashila Sani MEGA NURRIZALIA, MEGA Megga Ratnasari Pikoli Mellia Agustiyani Miranda Shafira Alamsyah Mirsa Umiyati Miryanti C, Miryanti Mohammad Rizky Afrillah Monica Christiani Muammar Kadafi Muchammad Ichwan Arif Muhaimin Muhaimin Muhamad Yulio Horison, Muhamad Yulio Muhammad Agung Faisal muhammad Al fata ramadhan Muhammad Nur Hakim Muhibbuddin Muhibbuddin Mulyaningrum, Meilia Mulyanto Widodo Mustaji Mustaji Mustikawati, Sri Mega N. M. F. Amin Mubarok Nabilla, Syifa Nadia Uluhiyah Muhammad Najih, Muhammad Najmi, Arnesa Aynun Nani Mulyati Natashya, Merina Lydia Isabella Natasia, Sri R. Nengsih, Yanti Karmila Ni Ketut Ayu Juliasih Ni Ketut Wiwiek Agustina Ni Made Rai Juniariani Ni Made Sila Ulati Ni Putu Sri Mariyatni Nico Octavian, Nico Nindi Aristi Nindya Rahmayanty Nining ARINI Ninis Agustini Damayani Nofha Rina Noor, Zaky Zamani Nova Robekha, Nova Nugroho Ajie Hartono Nur Aji Wibowo Nur Ihsan, Mega Farras i Nur Richana Nur, Amri Muliawan Nurcahya, Hilma Rizky Nurdin Nurdin Nuril Hafidz Fatih Al Farisy, Nuril Hafidz Nurjannah, Isna Fitri Nurma Risa Nurul Asiah Nurulwati, Nurulwati Nurusyifa, Delia Nurwahyuni Kiki Nuryah Asri Sjafirah Nuryah Asri Sjafirah, Nuryah Asri Nurzali Ismail Octa, Yasmine Oji Kurniadi Oji Kurniadi Oki Muraza Olfa Mega Otang Kurniaman P. S, Glorida Pae, Mychel Gatriser Paraswati, Vellyana Patria Domas G. Patricia, Yolanda Perbawasari, Susie Poppy Novita Rini Pramono, Anton Pranata, I Putu Gede Surya Angga Pranoto Samto Atmodjo Prastowo , Ari Agung Pratama, Abisha Akmal Praweswari, Mentari Priyanto, Muhammad Adi Priyatna, Centurion C Priyatna, Centurion C Priyo Subekti Priyo Subekti Priyo Subekti Priyo Subekti Priyo Subekti Priyo Subekti Priyo Subekti Priyo Subekti Prof. Dr. Gede Sedanayasa,M.Pd . PUJI LESTARI Purwaningsih, Ni Komang Purwanti Hadisiwi Puspita, Helen Putri Ani Putri Aprilia Putri Limilia Putri Limilia Putri, Risky Setiani Putu Nindhya Nirmala Qohar, Abd. R. Rijanta Radian Syauqii Rafiqoh Fitriani Rahma Triliana Rahma, Hana Haifa Rahmadhani, Callista Zharfa Rahmadhar, Yulia Rahmalya, Aryani Rahman Laba Rahmat Gaho Rahmawati, Riska Sri Rahmi Wellina Putri Raissa Az Zahra Ralampi, Dewi Agrianti Ramadhani Yanidar Ramdani, Adisti Citra Ramdhani, Bagoes Dwi Ramiah Ramiah Ramli Ahmad Rangga Saptya Mohamad Permana Ratmananda, Diasta Ratnasari, Eny Ratu Erlina Gentari Refnandes, Randy Renata Anisa Renyep Proborini, Renyep Resha, Firdhani Restianty, Ajani Reswita Reswita Retasari Dewi Retasari Dewi Retasari Dewi, Retasari Retna Astuti Kuswardani Ridho Berlianto Ridwan Setiawan Daradjat, dkk, Ridwan Setiawan Rinaldy, Jemmy Rinda Aunillah Sirait Rindu Twidi Bethary, Rindu Twidi Rinendaputri, Alyssa Azka Rio Wijianto, Rio Risa Desiana Syarif, Risa Desiana Rivy Valen Pabesak Riza A PUTRANTO Rizaldy Khair Rizka Humardewayanti Asdie Rizky Abdulah Rizky Pratama Hajati Rizqi Wahyu Pratama Rodianto, Rodianto Rohim, Mohammad Fatkur ROHMAN AL FALAHI, ABDUR Rohnulyanti, Hanim Roidisom, Shamit Romsyah Maryam Rorim Panday, Rorim Rosita, Eva Rossy FITRIA Rozalinda Rozalinda Ruchiat, Aat Rudi Masuswo Purwoko Rully Khairul Anwar Rupaka, Anggun Prima Gilang Rusmulyadi Rusmulyadi Rusydi Rusydi Ryanto Budiana, Heru Sa'diyah, Siti Rofik Atus Safi, Abdul Manan Saifur R, Saifur Saleha Radiah, Saleha Salsabila, Putriani Samosir, Deborah Sondang Napita Samsu Hadi, Samsu Santoso, Gatot Budi Saragih, Mega Fajri Aini Sarah Nurtyasrini Sarah Nurtyasrini, Sarah Sari, Nuraeni Nanda Satyadewi, Ajeng Jayanti Sayoga, I Putu Aldha Rasjman Sedarmayanti, Sedarmayanti Sediyono, Agung Seftia Rahmaning Tyas Septhian Marno Septian, Muhamad Fachry Septiani, Bernadeta Novita Septrian Adhi Saputro Setiarso Gunawan Setyastiawan, Ivan Setyowati, Setyowati Shabri Shabri, Shabri Shalhan Ridhan Warliana Sherly Amalia Shinta PERMATASARI Shintia NW Hardani Shofyan Tsauri Hermawan Sidauruk, Elsa Ernisa Valen Sihono Sihono Siti Karlinah Solly Aryza Sri Eko Wahyuni Sri Fauziyah Sri Harjanto Sri Murwanti Sri Sangkawati Sachro Sri Seti Indriani Sri Wayanti Sri Widodo Stovia, Anggita Subagyo, Razaqa Subaryono Subaryono Sudarno Sudarno Sudiyono Sudiyono Sudjianto Sudjianto, Sudjianto Suharno Suharno Suharyono Suharyono Suhri Hanafi Sukamto, Monique Sulaimiah, Sulaimiah Sularto Sularto Sultan Kemal Piagio Sumi Hudiyono Suryanti, Adetya Susanne Dida Susi Wahyuning Asih Susie Perbawasari Susmiati Susmiati Suwandi Sumartias Syaiful Bakhri Syamsudin Syamsudin Syauqy Lukman Syauqy Lukman Syauqy Lukman Syifa Fauziyah, Syifa Syifa Sharifa Rahmadani Syuhadak Syuhadak Syuhadak, Syuhadak T Haryono T W DARMONO Tahapari, Evi Tanditha, Tiara Kristiani Tanzil, Andini Qodriya Tarmizi Tarmizi Tasman Tasman Tasya Vicky Ryana Tatang Manggala Tedy Juliandhy Teguh Hadisukarno TIANA MILANDA Tiara Pascanoviera Robaeni Tiara Pascanoviera Robaeni Tiara Putri Effendy Tifanny Anggraini Tine Silvana Rachmawati Titi Kurniati Tiyas Tono Taufiq Tony Atyanto Dharoko, Tony Atyanto Tri Gunaedi Tri Panji Triadwinca, Brenda Trie Damayanti Umar Ma’ruf UP, Syefira Ichsan Uril Bahruddin, Uril Urip PERWITASARI Ute Lies Siti Khadijah Utomo, Sigit Prihanto Verena Agustini Viendra Salsabila Harahap Wada, Ibrahim Wahidin Teguh Sasongko Wahyu Caesarendra Wahyudi Wahyudi Wardana, Ruditya Yogi Wardani, Novita Eka Kusuma Wawan Setiawan Wendra Gandhatyasri Rohmah Weni Wahyuandari, Weni Widayanto, Widodo Widia Apriliani Widianto, Sunu Widodo Widayanto Widya Asmara Widyawati Hunta Wijaya, Viriyanata Wikut Deniarti, Wikut Wildana Wargadinata Wulan Purnama Sari Wuri Syaputri Yana Meliana Yani Lukmayani Yanti Setianti Yarza, Husnin Nahry Yasuhiro Mitani Yasuhiro Mitani, Yasuhiro Yazid Bindar Yogi W. Budhi Yonita, Lingga Yudhi Pratama Yudi Suryadi Yuliadi Yuliadi Yunia Dwie Nurchayanie Yusak Maryunianta Yusianto Yusianto, Yusianto Yusida Lusiana Yusniar Lubis Yusra, Wildan Yusrizal Yusrizal Yustikasari Yustikasari, Yustikasari Zahra Alifia Zahratul Millah Zahrial COTO Zahrotunnisa, Fathimah Zainal A. Wasaraka, Zainal A. Zifriyanthi Minanda Putri Zulfahmi Zulfahmi Zulfikar, M. Rizki Zulkarnain Zulkarnain Zulkifli N '

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